Isolated polynucleotides and polypeptides and methods of using same for increasing plant utility

ABSTRACT

Provided are isolated polypeptides comprising the amino acid sequence at least 80% homologous to SEQ ID NO:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754, 764-771 or 772 with the proviso that the amino acid sequence is not as set forth by SEQ ID NO: 765 or 771, isolated polynucleotides comprising the nucleic acid sequence at least 80% identical to SEQ ID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706, 716-719, 724-741 and 755-763 with the proviso that the nucleic acid sequence is not as set forth by SEQ ID NO:756 or 762, and isolated polynucleotides selected from the group consisting of SEQ ID NOs:779-792 and methods of using same for increasing oil content, yield, growth rate, biomass, vigor, abiotic stress tolerance and/or nitrogen use efficiency of a plant.

RELATED APPLICATIONS

This Application is a National Phase of PCT Patent Application No.PCT/IL2009/000508 having International filing date of May 21, 2009,which claims the benefit of U.S. Provisional Patent Application Nos.61/071,885 filed on May 22, 2008 and 61/129,296 filed on Jun. 17, 2008.The contents of the above Applications are all incorporated herein byreference.

FIELD AND BACKGROUND OF THE INVENTION

The present invention, in some embodiments thereof, relates topolypeptides, polynucleotides encoding same, transgenic plantsexpressing same and methods of producing and using same, and, moreparticularly, but not exclusively, to methods of increasing plant yield,oil yield, seed yield, biomass, growth rate, vigor, oil content, abioticstress tolerance and/or nitrogen use efficiency.

Abiotic stress conditions such as salinity, drought, flood, suboptimaltemperature and toxic chemical pollution, cause substantial damage toagricultural plants. Most plants have evolved strategies to protectthemselves against these conditions. However, if the severity andduration of the stress conditions are too great, the effects on plantdevelopment, growth and yield of most crop plants are profound.Furthermore, most of the crop plants are highly susceptible to abioticstress (ABS) and thus necessitate optimal growth conditions forcommercial crop yields. Continuous exposure to stress causes majoralterations in the plant metabolism which ultimately leads to cell deathand consequently yield losses.

The global shortage of water supply is one of the most severeagricultural problems affecting plant growth and crop yield and effortsare made to mitigate the harmful effects of desertification andsalinization of the world's arable land. Thus, Agbiotech companiesattempt to create new crop varieties which are tolerant to differentabiotic stresses focusing mainly in developing new varieties that cantolerate water shortage for longer periods.

Suboptimal nutrient (macro and micro nutrient) affect plant growth anddevelopment through the whole plant life cycle. One of the essentialmacronutrients for the plant is Nitrogen. Nitrogen is responsible forbiosynthesis of amino acids and nucleic acids, prosthetic groups, planthormones, plant chemical defenses, and the like. Nitrogen is often therate-limiting element in plant growth and all field crops have afundamental dependence on inorganic nitrogenous fertilizer. Sincefertilizer is rapidly depleted from most soil types, it must be suppliedto growing crops two or three times during the growing season.Additional important macronutrients are Phosphorous (P) and Potassium(K), which have a direct correlation to yield and general planttolerance.

Vegetable or seed oils are the major source of energy and nutrition inhuman and animal diet. They are also used for the production ofindustrial products, such as paints, inks and lubricants. In addition,plant oils represent renewable sources of long-chain hydrocarbons whichcan be used as fuel. Since the currently used fossil fuels are finiteresources and are gradually being depleted, fast growing biomass cropsmay be used as alternative fuels or for energy feedstocks and may reducethe dependence on fossil energy supplies. However, the major bottleneckfor increasing consumption of plant oils as bio-fuel is the oil price,which is still higher than fossil fuel [eia (dot) doe (dot)gov/oiaf/analysispaper/biodiesel/; njbiz(dot)com/weekly_article.asp?aID=19755147 (dot) 6122555 (dot) 957931(dot) 7393254 (dot) 4337383 (dot) 561&aID2=73678]. In addition, theproduction rate of plant oil is limited by the availability ofagricultural land and water. Thus, increasing plant oil yields from thesame growing area can effectively overcome the shortage in productionspace and can decrease vegetable oil prices at the same time.

Studies aiming at increasing plant oil yields focus on theidentification of genes involved in oil metabolism as well as in genescapable of increasing plant and seed yields in transgenic plants.

Genes known to be involved in increasing plant oil yields include thoseparticipating in fatty acid synthesis or sequestering such as desaturase[e.g., DELTA6, DELTA12 or acyl-ACP (Ssi2; Arabidopsis InformationResource (TAIR; arabidopsis (dot) org/), TAIR No. AT2G43710)], OleosinA(TAIR No. AT3G01570) or FAD3 (TAIR No. AT2G29980), and varioustranscription factors and activators such as Lec1 [TAIR No. AT1G21970,Lotan et al. 1998. Cell. 26; 93(7):1195-205], Lec2 [TAIR No. AT1G28300,Santos Mendoza et al. 2005, FEBS Lett. 579(20:4666-70], Fus3 (TAIR No.AT3G26790), ABI3 [TAIR No. AT3G24650, Lara et al. 2003. J Biol Chem.278(23): 21003-11] and Wri1 [TAIR No. AT3G54320, Cernac and Benning,2004. Plant J. 40(4): 575-85].

Zabrouskov V., et al., 2002 (Physiol Plant. 116:172-185) describe anincrease in the total lipid fraction by upregulation of endoplasmicreticulum (FAD3) and plastidal (FAD7) fatty acid desaturases in potato.

Wang H W et al., 2007 (Plant J. 52:716-29. Epub 2007 Sep. 18) describean increase in the content of total fatty acids and lipids in plantseeds by over-expressing the GmDof4 and GmDof11 transcription factors.

Vigeolas H, et al. [Plant Biotechnol J. 2007, 5(3):431-41] and U.S. Pat.Appl. No. 20060168684 discloses an increase in seed oil content inoil-seed rape (Brassica napus L.) by over-expression of a yeastglycerol-3-phosphate dehydrogenase under the control of a seed-specificpromoter.

Katavic V, et al., 2000 (Biochem Soc Trans. 28:935-7) describe the useof the Arabidopsis FAE1 and yeast SLC1-1 genes for improvements inerucic acid and oil content in rapeseed.

U.S. Pat. Appl. No. 20080076179 discloses an isolated moss nucleic acidencoding a lipid metabolism protein (LMP) and transgenic plantsexpressing same with increased lipid levels.

U.S. Pat. Appl. No. 20060206961 discloses a method of increasing oilcontent in plants (e.g., in plant seeds), by expressing in the plant theYpr140w polypeptide.

U.S. Pat. Appl. No. 20060174373 discloses a method of increasing oilcontent in plants by expressing a nucleic acid encoding atriacylglycerols (TAG) synthesis enhancing protein (TEP) in the plant.

U.S. Pat. Appl. Nos. 20070169219, 20070006345, 20070006346 and20060195943, disclose transgenic plants with improved nitrogen useefficiency which can be used for the conversion into fuel or chemicalfeedstocks.

WO2004/104162 teaches polynucleotide sequences and methods of utilizingsame for increasing the tolerance of a plant to abiotic stresses and/orincreasing the biomass of a plant.

WO2007/020638 teaches polynucleotide sequences and methods of utilizingsame for increasing the tolerance of a plant to abiotic stresses and/orincreasing the biomass, vigor and/or yield of a plant.

WO2008/122890 teaches polynucleotide sequences and methods of utilizingsame for increasing oil content, growth rate, biomass, yield and/orvigor of a plant.

SUMMARY OF THE INVENTION

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing oil content, yield, growthrate, biomass, vigor, abiotic stress tolerance and/or nitrogen useefficiency of a plant, comprising expressing within the plant anexogenous polynucleotide comprising a nucleic acid sequence at least 80%identical to SEQ ID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706,716-719, 724-741 and 755-763 with the proviso that the nucleic acidsequence is not as set forth by SEQ ID NO:756 or 762, thereby increasingthe oil content, yield, growth rate, biomass, vigor, abiotic stresstolerance and/or nitrogen use efficiency of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing oil content, yield, growthrate, biomass, vigor, abiotic stress tolerance and/or nitrogen useefficiency of a plant, comprising expressing within the plant anexogenous polynucleotide comprising the nucleic acid sequence selectedfrom the group consisting of SEQ ID NOs:18, 1-16, 19-50, 101-378,657-672, 674-706, 716-719, 724-741, 755, 757-761 and 763, therebyincreasing the oil content, yield, growth rate, biomass, vigor, abioticstress tolerance and/or nitrogen use efficiency of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing oil content, yield, growthrate, biomass, vigor, abiotic stress tolerance and/or nitrogen useefficiency of a plant, comprising transforming the plant with anexogenous polynucleotide capable of downregulating the expression levelof a nucleic acid sequence at least 80% identical to SEQ ID NO:17 or673, thereby increasing the oil content, yield, growth rate, biomass,vigor, abiotic stress tolerance and/or nitrogen use efficiency of theplant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing oil content, yield, growthrate, biomass, vigor, abiotic stress tolerance and/or nitrogen useefficiency of a plant, comprising transforming the plant with anexogenous polynucleotide capable of downregulating the expression levelof the nucleic acid sequence set forth in SEQ ID NO:17 or 673, therebyincreasing the oil content, yield, growth rate, biomass, vigor, abioticstress tolerance and/or nitrogen use efficiency of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of producing oil, comprising: (a) providingthe plant according to the method of the invention; and (b) extractingthe oil from the plant; thereby producing the oil.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence at least 80% identical to SEQ ID NOs:18, 1-16, 19-50, 101-378,657-672, 674-706, 716-719, 724-741 and 755-763 with the proviso that thenucleic acid sequence is not as set forth by SEQ ID NO:756 or 762.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising the nucleic acidsequence selected from the group consisting of SEQ ID NOs:18, 1-16,19-50, 101-378, 657-672, 674-706, 716-719, 724-741, 755, 757-761 and763.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence capable of downregulating the expression level of a nucleicacid sequence at least 80% identical to SEQ ID NO:17 or 673.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence capable of downregulating the expression level of the nucleicacid sequence set forth in SEQ ID NO:17 or 673.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence encoding a polypeptide which comprises an amino acid sequenceat least 80% homologous to the amino acid sequence set forth in SEQ IDNOs:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754 and 764-772with the proviso that the amino acid sequence is not as set forth by SEQID NO: 765 or 771.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence encoding a polypeptide which comprises the amino acid sequenceselected from the group consisting of SEQ ID NOs: 68, 51-66, 69-100,379-656, 707-715, 720-723, 742-754, 764, 766-770 and 772.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence capable of downregulating the expression level or activity of apolypeptide at least 80% homologous to the polypeptide set forth by SEQID NO:67.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence capable of downregulating the expression level or activity ofthe polypeptide set forth by SEQ ID NO:67.

According to an aspect of some embodiments of the present inventionthere is provided a nucleic acid construct comprising the isolatedpolynucleotide of the invention, and a promoter for directingtranscription of the nucleic acid sequence in a host cell.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polypeptide comprising an amino acidsequence at least 80% homologous to SEQ ID NO:68, 51-66, 69-100,379-656, 707-715, 720-723, 742-754, 764-771 or 772 with the proviso thatthe amino acid sequence is not as set forth by SEQ ID NO:765 or 771.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polypeptide comprising the amino acidsequence selected from the group consisting of SEQ ID NOs: 68, 51-66,69-100, 379-656, 707-715, 720-723, 742-754, 764, 766-770 and 772.

According to an aspect of some embodiments of the present inventionthere is provided a nucleic acid construct comprising a nucleic acidsequence selected from the group consisting of SEQ ID NOs:779-792 and aheterologous polynucleotide sequence, wherein the nucleic acid sequenceis capable of regulating expression of the heterologous polynucleotidein a host cell.

According to some embodiments of the invention, the heterologouspolynucleotide is a reporter gene.

According to some embodiments of the invention, regulating theexpression of the heterologous polynucleotide is in a tissue specificmanner.

According to some embodiments of the invention, regulating theexpression of the heterologous polynucleotide is in a developmentalstage—specific manner.

According to some embodiments of the invention, the heterologouspolynucleotide comprises a nucleic acid sequence at least 80% identicalto the nucleic acid sequence selected from the group consisting of SEQID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706, 716-719, 724-741 and755-763 with the proviso that the nucleic acid sequence is not as setforth by SEQ ID NO:756 or 762.

According to some embodiments of the invention, the heterologouspolynucleotide encodes an amino acid sequence at least at least 80%homologous to SEQ ID NOs:68, 51-66, 69-100, 379-656, 707-715, 720-723,742-754 and 764-772 with the proviso that the amino acid sequence is notas set forth by SEQ ID NO: 765 or 771.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing oil content, yield, growthrate, biomass, vigor, abiotic stress tolerance and/or nitrogen useefficiency of a plant comprising expressing within the plant the nucleicacid construct of claim 17, wherein the heterologous polynucleotidecomprises a nucleic acid sequence at least 80% identical to SEQ IDNOs:18, 1-16, 19-50, 101-378, 657-672, 674-706, 716-719, 724-741 and755-763 with the proviso that the nucleic acid sequence is not as setforth by SEQ ID NO:756 or 762, thereby increasing the oil content,yield, growth rate, biomass, vigor, abiotic stress tolerance and/ornitrogen use efficiency of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing oil content, yield, growthrate, biomass, vigor, abiotic stress tolerance and/or nitrogen useefficiency of a plant comprising expressing within the plant the nucleicacid construct of claim 17, wherein the heterologous polynucleotideencodes an amino acid sequence at least at least 80% homologous to SEQID NOs:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754 and 764-772with the proviso that the amino acid sequence is not as set forth by SEQID NO: 765 or 771, thereby increasing the oil content, yield, growthrate, biomass, vigor, abiotic stress tolerance and/or nitrogen useefficiency of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a plant cell exogenously expressing the polynucleotideof the invention, or the nucleic acid construct of the invention.

According to an aspect of some embodiments of the present inventionthere is provided a plant cell exogenously expressing the polypeptide ofthe invention.

According to some embodiments of the invention, the nucleic acidsequence is as set forth in SEQ ID NO:18, 1-16, 19-50, 101-378, 657-672,674-706, 716-719, 724-741, 755, 757-761 or 763.

According to some embodiments of the invention, the polynucleotideconsists of the nucleic acid sequence selected from the group consistingof SEQ ID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706, 716-719,724-741, 755, 757-761 and 763.

According to some embodiments of the invention, the nucleic acidsequence encodes an amino acid sequence at least 80% homologous to SEQID NO:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754, 764-771 or772 with the proviso that the amino acid sequence is not as set forth bySEQ ID NO: 765 or 771.

According to some embodiments of the invention, the nucleic acidsequence encodes the amino acid sequence selected from the groupconsisting of SEQ ID NOs: 68, 51-66, 69-100, 379-656, 707-715, 720-723,742-754, 764, 766-770 and 772.

According to some embodiments of the invention, the plant cell formspart of a plant.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder the abiotic stress.

According to some embodiments of the invention, the abiotic stress isselected from the group consisting of salinity, drought, waterdeprivation, low temperature, high temperature, heavy metal toxicity,anaerobiosis, nutrient deficiency, nutrient excess, atmosphericpollution and UV irradiation.

According to some embodiments of the invention, the polynucleotide is aco-suppression polynucleotide, an antisense polynucleotide, anRNA-interference polynucleotide or a Ribozyme polynucleotide.

BRIEF DESCRIPTION OF THE DRAWINGS

Some embodiments of the invention are herein described, by way ofexample only, with reference to the accompanying drawings. With specificreference now to the drawings in detail, it is stressed that theparticulars shown are by way of example and for purposes of illustrativediscussion of embodiments of the invention. In this regard, thedescription taken with the drawings makes apparent to those skilled inthe art how embodiments of the invention may be practiced.

In the drawings:

FIGS. 1A-D are digital images of leaves depicting leaves parameters.FIG. 1A—leaf length (the leaf length is represented by the arrow); FIG.1B—laminar length (the laminar length is represented by the arrow); FIG.1C—laminar area (the laminar area is represented by the white ellipse);FIG. 1D—laminar width (the laminar width is represented by the arrow).Blade circularity was calculated as laminar width divided by laminarlength.

FIGS. 2A-B are images depicting visualization of root development ofplants grown in transparent agar plates. The different transgenes weregrown in transparent agar plates for 17 days and the plates werephotographed every 2 days starting at day 7. FIG. 2A—An image of aphotograph of plants taken following 12 days on agar plates. FIG. 2B—Animage of root analysis in which the length of the root measured isrepresented by a red arrow.

FIG. 3 is a schematic illustration of the pGI binary plasmid used forexpressing the isolated polynucleotide sequences of the invention.RB—T-DNA right border; LB—T-DNA left border; H—HindIII restrictionenzyme; X—XbaI restriction enzyme; B—BamHI restriction enzyme; S—Sailrestriction enzyme; Sm—SmaI restriction enzyme; R-I—EcoRI restrictionenzyme; Sc—SacI/SstI/Ecl136II; (numbers)—Length in base-pairs; NOSpro=nopaline synthase promoter; NPT-II=neomycin phosphotransferase gene;NOS ter=nopaline synthase terminator; Poly-A signal (polyadenylationsignal); GUSintron—the GUS reporter gene (coding sequence and intron).The isolated polynucleotide sequences of some embodiments of theinvention were cloned into the vector while replacing the GUSintronreporter gene.

DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION

The present invention, in some embodiments thereof, relates to isolatedpolypeptides and polynucleotides encoding same, and more particularly,but not exclusively, to methods of using same for increasing oilcontent, growth rate, yield, biomass, vigor, abiotic stress toleranceand/or nitrogen use efficiency of a plant.

Before explaining at least one embodiment of the invention in detail, itis to be understood that the invention is not necessarily limited in itsapplication to the details set forth in the following description orexemplified by the Examples. The invention is capable of otherembodiments or of being practiced or carried out in various ways.

While reducing the present invention to practice, the present inventorshave identified novel polypeptides and polynucleotides which can be usedto increase yield, growth rate, biomass, oil content, vigor, abioticstress tolerance and/or nitrogen use efficiency of a plant.

Thus, as shown in the Examples section which follows, the presentinventors have utilized bioinformatics tools to identify polynucleotideswhich enhance yield (e.g., seed yield, oil yield and oil content),growth rate, biomass, vigor, abiotic stress tolerance and/or nitrogenuse efficiency of a plant. Genes which affect the trait-of-interest wereidentified based on expression profiles of genes of several Arabidopsisecotypes and tissues, homology with genes known to affect thetrait-of-interest and using digital expression profile in specifictissues and conditions (Tables 1, 3, 4, 5, 6, 7, 8, 9, 10 and 11,Examples 1 and 3). Homologous polypeptides and polynucleotides havingthe same function were also identified (Table 2, Example 2). Transgenicplants over-expressing the identified polynucleotides exhibit increasedseed yield (e.g., weight of 1000 seeds), oil yield (e.g., oil percentagein seed), biomass (e.g., dry matter), harvest index, growth rate,rosette area, abiotic stress tolerance (e.g., to drought conditions) andnitrogen use efficiency (Tables 22, 23, 24, 25, 26 and 27; Examples 5and 7; Tables 28-30, Example 8). In addition, the present inventors haveuncovered that polynucleotides which reduce the expression level and/oractivity of certain gene products (e.g., the BDL127 gene; SEQ ID NO:17or 673) can increase yield (e.g., seed yield), biomass and/or growthrate in plants (Tables 31-36; Example 9). As is further shown in theExamples section which follows, the present inventors have uncoverednovel promoter sequences which can be used to express thegene-of-interest in a tissue specific and/or developmentalstage-specific manner (Tables 16, 17, 18, 19, 20 and 21, Example 6).Altogether, these results suggest the use of the polynucleotides orpolypeptides of the invention for increasing yield, growth rate,biomass, vigor, abiotic stress tolerance and/or nitrogen use efficiencyof a plant

Thus, according to an aspect of some embodiments of the invention, thereis provided method of increasing oil content, yield, growth rate,biomass, vigor, abiotic stress tolerance and/or nitrogen use efficiencyof a plant, comprising expressing within the plant an exogenouspolynucleotide comprising a nucleic acid sequence at least 80% identicalto SEQ ID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706, 716-719,724-741 and 755-763 with the proviso that the nucleic acid sequence isnot as set forth by SEQ ID NO:756 or 762, thereby increasing the oilcontent, yield, growth rate, biomass, vigor, abiotic stress toleranceand/or nitrogen use efficiency of the plant.

The phrase “oil content” as used herein refers to the amount of lipidsin a given plant organ, either the seeds (seed oil content) or thevegetative portion of the plant (vegetative oil content) and istypically expressed as percentage of dry weight (10% humidity of seeds)or wet weight (for vegetative portion).

It should be noted that oil content is affected by intrinsic oilproduction of a tissue (e.g., seed, vegetative portion), as well as themass or size of the oil-producing tissue per plant or per growth period.

In one embodiment, increase in oil content of the plant can be achievedby increasing the size/mass of a plant's tissue(s) which comprise oilper growth period. Thus, increased oil content of a plant can beachieved by increasing the yield, growth rate, biomass and vigor of theplant.

As used herein the phrase “plant yield” refers to the amount (asdetermined by weight or size) or quantity (numbers) of tissue producedper plant or per growing season. Hence increased yield could affect theeconomic benefit one can obtain from the plant in a certain growing areaand/or growing time.

It should be noted that a plant yield can be affected by variousparameters including, but not limited to, plant biomass; plant vigor;growth rate; seed yield; oil yield; content of oil, starch and/orprotein in harvested organs (e.g., seeds or vegetative parts of theplant); number of flowers (florets) per panicle (expressed as a ratio ofnumber of filled seeds over number of primary panicles); harvest index;number of plants grown per area; number and size of harvested organs perplant and per area; number of plants per growing area (density); numberof harvested organs in field; total leaf area; carbon assimilation andcarbon partitioning (the distribution/allocation of carbon within theplant); resistance to shade; number of harvestable organs (e.g. seeds),seeds per pod, weight per seed; and modified architecture [such asincrease stalk diameter, thickness or improvement of physical properties(e.g. elasticity)].

As used herein the phrase “plant biomass” refers to the amount (measuredin grams of air-dry tissue) of a tissue produced from the plant in agrowing season, which could also determine or affect the plant yield orthe yield per growing area. An increase in plant biomass can be in thewhole plant or in parts thereof such as aboveground (harvestable) parts,vegetative biomass, roots and seeds.

As used herein the phrase “growth rate” refers to the increase in plantorgan size per time (can be measured in cm² per day).

As used herein the phrase “plant vigor” refers to the amount (measuredby weight) of tissue produced by the plant in a given time. Henceincreased vigor could determine or affect the plant yield or the yieldper growing time or growing area. In addition, early vigor (seed and/orseedling) result with improved field stand.

As used herein the phrase “seed yield” refers to the number or weight ofthe seeds per plant, seeds per pod, or per growing area or to the weightof a single seed, or to the oil extracted per seed. Hence seed yield canbe affected by seed dimensions (e.g., length, width, perimeter, areaand/or volume), number of (filled) seeds and seed filling rate and byseed oil content. Hence increase seed yield per plant could affect theeconomic benefit one can obtain from the plant in a certain growing areaand/or growing time; and increase seed yield per growing area could beachieved by increasing seed yield per plant, and/or by increasing numberof plants grown on the same given area.

The term “seed” (also referred to as “grain” or “kernel”) as used hereinrefers to a small embryonic plant enclosed in a covering called the seedcoat (usually with some stored food), the product of the ripened ovuleof gymnosperm and angiosperm plants which occurs after fertilization andsome growth within the mother plant.

It should be noted that a plant yield can be determined under stress(e.g., abiotic stress, nitrogen-limiting conditions) or non-stress(normal) conditions.

As used herein, the phrase “non-stress conditions” refers to the growthconditions (e.g., water, temperature, light-dark cycles, humidity, saltconcentration, fertilizer concentration in soil, nutrient supply such asnitrogen, phosphorous and/or potassium), which enable normal metabolism,growth, reproduction and/or viability of a plant at any stage in itslife cycle (from seed to mature plant and back to seed again). It shouldbe noted that while the non-stress conditions may include some mildvariations from the optimal conditions (which vary from one type/speciesof a plant to another), such variations do not cause the plant to ceasegrowing without the capacity to resume growth.

The phrase “abiotic stress” as used herein refers to any adverse effecton metabolism, growth, reproduction and/or viability of a plant.Accordingly, abiotic stress can be induced by suboptimal environmentalgrowth conditions such as, for example, salinity, water deprivation,flooding, freezing, low or high temperature, heavy metal toxicity,anaerobiosis, nutrient deficiency, atmospheric pollution or UVirradiation. The implications of abiotic stress are discussed in theBackground section.

The phrase “abiotic stress tolerance” as used herein refers to theability of a plant to endure an abiotic stress without suffering asubstantial alteration in metabolism, growth, productivity and/orviability.

As used herein the phrase “nitrogen use efficiency (NUE)” refers to themetabolic process(es) which lead to an increase in the plant's yield,biomass, vigor, growth rate and abiotic stress tolerance per nitrogenunit applied. The metabolic process can be the uptake, spread,absorbent, accumulation, relocation (within the plant) and use ofnitrogen absorbed by the plant.

As used herein the phrase “nitrogen-limiting conditions” refers togrowth conditions which include a level (e.g., concentration) ofnitrogen (e.g., ammonium or nitrate) applied which is below the levelneeded for normal plant metabolism, growth, reproduction and/orviability.

As used herein the term “increasing” refers to at least about 2%, atleast about 3%, at least about 4%, at least about 5%, at least about10%, at least about 15%, at least about 20%, at least about 30%, atleast about 40%, at least about 50%, at least about 60%, at least about70%, at least about 80%, increase in oil content, yield, growth rate,biomass, vigor, abiotic stress tolerance and/or nitrogen use efficiencyof a plant as compared to a native plant [i.e., a plant not modifiedwith the biomolecules (polynucleotide or polypeptides) of the invention,e.g., a non-transformed plant of the same species which is grown underthe same growth conditions).

As used herein, the phrase “exogenous polynucleotide” refers to aheterologous nucleic acid sequence which may not be naturally expressedwithin the plant or which overexpression in the plant is desired. Theexogenous polynucleotide may be introduced into the plant in a stable ortransient manner, so as to produce a ribonucleic acid (RNA) moleculeand/or a polypeptide molecule. It should be noted that the exogenouspolynucleotide may comprise a nucleic acid sequence which is identicalor partially homologous to an endogenous nucleic acid sequence of theplant.

According to some embodiments of the invention the exogenouspolynucleotide comprises a nucleic acid sequence which is at least about80%, at least about 81%, at least about 82%, at least about 83%, atleast about 84%, at least about 85%, at least about 86%, at least about87%, at least about 88%, at least about 89%, at least about 90%, atleast about 91%, at least about 92%, at least about 93%, at least about93%, at least about 94%, at least about 95%, at least about 96%, atleast about 97%, at least about 98%, at least about 99%, e.g., 100%identical to the nucleic acid sequence selected from the groupconsisting of SEQ ID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706,716-719, 724-741 and 755-763 with the proviso that the nucleic acidsequence is not as set forth by SEQ ID NO:756 or 762.

Identity (e.g., percent homology) can be determined using any homologycomparison software, including for example, the BlastN software of theNational Center of Biotechnology Information (NCBI) such as by usingdefault parameters.

According to some embodiments of the invention the exogenouspolynucleotide is at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 93%, at least about 94%, at least about95%, at least about 96%, at least about 97%, at least about 98%, atleast about 99%, e.g., 100% identical to the polynucleotide selectedfrom the group consisting of SEQ ID NOs:18, 1-16, 19-50, 101-378,657-672, 674-706, 716-719, 724-741 and 755-763 with the proviso that theexogenous polynucleotide is not as set forth by SEQ ID NO:756 or 762.

According to some embodiments of the invention the exogenouspolynucleotide is set forth by SEQ ID NO:18, 1-16, 19-50, 101-378,657-672, 674-706, 716-719, 724-741, 755, 757-761 or 763.

In exemplary embodiments the exogenous polynucleotide is not thepolynucleotide set forth by SEQ ID NO:807 or 808.

As used herein the term “polynucleotide” refers to a single or doublestranded nucleic acid sequence which is isolated and provided in theform of an RNA sequence, a complementary polynucleotide sequence (cDNA),a genomic polynucleotide sequence and/or a composite polynucleotidesequences (e.g., a combination of the above).

The term “isolated” refers to at least partially separated from thenatural environment e.g., from a plant cell.

As used herein the phrase “complementary polynucleotide sequence” refersto a sequence, which results from reverse transcription of messenger RNAusing a reverse transcriptase or any other RNA dependent DNA polymerase.Such a sequence can be subsequently amplified in vivo or in vitro usinga DNA dependent DNA polymerase.

As used herein the phrase “genomic polynucleotide sequence” refers to asequence derived (isolated) from a chromosome and thus it represents acontiguous portion of a chromosome.

As used herein the phrase “composite polynucleotide sequence” refers toa sequence, which is at least partially complementary and at leastpartially genomic. A composite sequence can include some exonalsequences required to encode the polypeptide of the present invention,as well as some intronic sequences interposing therebetween. Theintronic sequences can be of any source, including of other genes, andtypically will include conserved splicing signal sequences. Suchintronic sequences may further include cis acting expression regulatoryelements.

According to some embodiments of the invention, the exogenouspolynucleotide of the invention encodes a polypeptide having an aminoacid sequence at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 94%, at least about 95%, at least about96%, at least about 97%, at least about 98%, at least about 99%, or moresay 100% homologous to the amino acid sequence selected from the groupconsisting of SEQ ID NOs:68, 51-66, 69-100, 379-656, 707-715, 720-723,742-754 and 764-772 with the proviso that the amino acid sequence is notas set forth by SEQ ID NO: 765 or 771.

Homology (e.g., percent homology) can be determined using any homologycomparison software, including for example, the BlastP or TBLASTNsoftware of the National Center of Biotechnology Information (NCBI) suchas by using default parameters, when starting from a polypeptidesequence; or the tBLASTX algorithm (available via the NCBI) such as byusing default parameters, which compares the six-frame conceptualtranslation products of a nucleotide query sequence (both strands)against a protein sequence database.

Homologous sequences include both orthologous and paralogous sequences.The term “paralogous” relates to gene-duplications within the genome ofa species leading to paralogous genes. The term “orthologous” relates tohomologous genes in different organisms due to ancestral relationship.

One option to identify orthologues in monocot plant species is byperforming a reciprocal blast search. This may be done by a first blastinvolving blasting the sequence-of-interest against any sequencedatabase, such as the publicly available NCBI database which may befound at: ncbi (dot) nlm (dot) nih (dot) gov. If orthologues in ricewere sought, the sequence-of-interest would be blasted against, forexample, the 28,469 full-length cDNA clones from Oryza sativa cv.Nipponbare available at NCBI. The blast results may be filtered. Thefull-length sequences of either the filtered results or the non-filteredresults are then blasted back (second blast) against the sequences ofthe organism from which the sequence-of-interest is derived. The resultsof the first and second blasts are then compared. An orthologue isidentified when the sequence resulting in the highest score (best hit)in the first blast identifies in the second blast the query sequence(the original sequence-of-interest) as the best hit. Using the samerational a paralogue (homolog to a gene in the same organism) is found.In case of large sequence families, the ClustalW program may be used[ebi (dot) ac (dot) uk/Tools/clustalw2/index (dot) html], followed by aneighbor-joining tree (en (dot) wikipedia (dot)org/wiki/Neighbor-joining) which helps visualizing the clustering.

In an exemplary embodiment the exogenous polynucleotide does not encodea polypeptide having the amino acid sequence selected from the groupconsisting of SEQ ID NOs: 809-852.

According to some embodiments of the invention, the exogenouspolynucleotide encodes a polypeptide consisting of the amino acidsequence set forth by SEQ ID NO: 68, 51-66, 69-100, 379-656, 707-715,720-723, 742-754, 764, 766-770 or 772.

Nucleic acid sequences encoding the polypeptides of the presentinvention may be optimized for expression. Non-limiting examples ofoptimized nucleic acid sequences are provided in SEQ ID NOs:663(BDL-113), 675 (BDL-129), 676 (BDL-130), 680 (BDL-134), 683 (BDL-137),684 (BDL-139) and 685 (BDL-141) which encode optimized polypeptidecomprising the amino acid sequences set forth by SEQ ID NOs:57, 69, 712,74, 77, 78 and 79. Examples of such sequence modifications include, butare not limited to, an altered G/C content to more closely approach thattypically found in the plant species of interest, and the removal ofcodons atypically found in the plant species commonly referred to ascodon optimization.

The phrase “codon optimization” refers to the selection of appropriateDNA nucleotides for use within a structural gene or fragment thereofthat approaches codon usage within the plant of interest. Therefore, anoptimized gene or nucleic acid sequence refers to a gene in which thenucleotide sequence of a native or naturally occurring gene has beenmodified in order to utilize statistically-preferred orstatistically-favored codons within the plant. The nucleotide sequencetypically is examined at the DNA level and the coding region optimizedfor expression in the plant species determined using any suitableprocedure, for example as described in Sardana et al. (1996, Plant CellReports 15:677-681). In this method, the standard deviation of codonusage, a measure of codon usage bias, may be calculated by first findingthe squared proportional deviation of usage of each codon of the nativegene relative to that of highly expressed plant genes, followed by acalculation of the average squared deviation. The formula used is: 1SDCU=n=1 N[(Xn−Yn)/Yn]2/N, where Xn refers to the frequency of usage ofcodon n in highly expressed plant genes, where Yn to the frequency ofusage of codon n in the gene of interest and N refers to the totalnumber of codons in the gene of interest. A Table of codon usage fromhighly expressed genes of dicotyledonous plants is compiled using thedata of Murray et al. (1989, Nuc Acids Res. 17:477-498).

One method of optimizing the nucleic acid sequence in accordance withthe preferred codon usage for a particular plant cell type is based onthe direct use, without performing any extra statistical calculations,of codon optimization Tables such as those provided on-line at the CodonUsage Database through the NIAS (National Institute of AgrobiologicalSciences) DNA bank in Japan (kazusa (dot) or (dot) jp/codon/). The CodonUsage Database contains codon usage tables for a number of differentspecies, with each codon usage Table having been statisticallydetermined based on the data present in Genbank.

By using the above Tables to determine the most preferred or mostfavored codons for each amino acid in a particular species (for example,rice), a naturally-occurring nucleotide sequence encoding a protein ofinterest can be codon optimized for that particular plant species. Thisis effected by replacing codons that may have a low statisticalincidence in the particular species genome with corresponding codons, inregard to an amino acid, that are statistically more favored. However,one or more less-favored codons may be selected to delete existingrestriction sites, to create new ones at potentially useful junctions(5′ and 3′ ends to add signal peptide or termination cassettes, internalsites that might be used to cut and splice segments together to producea correct full-length sequence), or to eliminate nucleotide sequencesthat may negatively effect mRNA stability or expression.

The naturally-occurring encoding nucleotide sequence may already, inadvance of any modification, contain a number of codons that correspondto a statistically-favored codon in a particular plant species.Therefore, codon optimization of the native nucleotide sequence maycomprise determining which codons, within the native nucleotidesequence, are not statistically-favored with regards to a particularplant, and modifying these codons in accordance with a codon usage tableof the particular plant to produce a codon optimized derivative. Amodified nucleotide sequence may be fully or partially optimized forplant codon usage provided that the protein encoded by the modifiednucleotide sequence is produced at a level higher than the proteinencoded by the corresponding naturally occurring or native gene.Construction of synthetic genes by altering the codon usage is describedin for example PCT Patent Application 93/07278.

Thus, the invention encompasses nucleic acid sequences describedhereinabove; fragments thereof, sequences hybridizable therewith,sequences homologous thereto, sequences encoding similar polypeptideswith different codon usage, altered sequences characterized bymutations, such as deletion, insertion or substitution of one or morenucleotides, either naturally occurring or man induced, either randomlyor in a targeted fashion.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 93%, at least about 94%, at least about95%, at least about 96%, at least about 97%, at least about 98%, atleast about 99%, e.g., 100% identical to the polynucleotide selectedfrom the group consisting of SEQ ID NOs:18, 1-16, 19-50, 101-378,657-672, 674-706, 716-719, 724-741 and 755-763 with the proviso that thenucleic acid sequence is not as set forth by SEQ ID NO:756 or 762.

According to some embodiments of the invention the isolatedpolynucleotide comprising the nucleic acid sequence selected from thegroup consisting of SEQ ID NOs:18, 1-16, 19-50, 101-378, 657-672,674-706, 716-719, 724-741, 755, 757-761 and 763.

According to some embodiments of the invention the isolatedpolynucleotide is set forth by SEQ ID NO:18, 1-16, 19-50, 101-378,657-672, 674-706, 716-719, 724-741, 755, 757-761 or 763.

In exemplary embodiments the isolated polynucleotide is not thepolynucleotide set forth by SEQ ID NO:807 or 808.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence encoding a polypeptide which comprises an amino acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%, or more say 100% homologous to the amino acid sequence selectedfrom the group consisting of SEQ ID NOs:68, 51-66, 69-100, 379-656,707-715, 720-723, 742-754 and 764-772 with the proviso that the aminoacid sequence is not as set forth by SEQ ID NO: 765 or 771.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence encoding a polypeptide which comprises the amino acidsequence selected from the group consisting of SEQ ID NOs:68, 51-66,69-100, 379-656, 707-715, 720-723, 742-754, 764, 766-770 and 772.

The invention provides an isolated polypeptide comprising an amino acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%, or more say 100% homologous to an amino acid sequence selected fromthe group consisting of SEQ ID NOs:68, 51-66, 69-100, 379-656, 707-715,720-723, 742-754 and 764-772 with the proviso that the amino acidsequence is not as set forth by SEQ ID NO: 765 or 771.

In an exemplary embodiment the polypeptide is not the polypeptide setforth by SEQ ID NO: 809-851 or 852.

According to some embodiments of the invention, the polypeptidecomprising an amino acid sequence selected from the group consisting ofSEQ ID SEQ ID NOs:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754,764, 766-770 and 772.

According to some embodiments of the invention, the polypeptide is setforth by SEQ ID NO: 68, 51-66, 69-100, 379-656, 707-715, 720-723,742-754, 764, 766-770 or 772.

The invention also encompasses fragments of the above describedpolypeptides and polypeptides having mutations, such as deletions,insertions or substitutions of one or more amino acids, either naturallyoccurring or man induced, either randomly or in a targeted fashion.

The term “plant” as used herein encompasses whole plants, ancestors andprogeny of the plants and plant parts, including seeds, shoots, stems,roots (including tubers), and plant cells, tissues and organs. The plantmay be in any form including suspension cultures, embryos, meristematicregions, callus tissue, leaves, gametophytes, sporophytes, pollen, andmicrospores. Plants that are particularly useful in the methods of theinvention include all plants which belong to the superfamilyViridiplantae, in particular monocotyledonous and dicotyledonous plantsincluding a fodder or forage legume, ornamental plant, food crop, tree,or shrub selected from the list comprising Acacia spp., Acer spp.,Actinidia spp., Aesculus spp., Agathis australis, Albizia amara,Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, Asteliafragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassicaspp., Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadabafarinosa, Calliandra spp, Camellia sinensis, Canna indica, Capsicumspp., Cassia spp., Centroema pubescens, Chacoomeles spp., Cinnamomumcassia, Coffea arabica, Colophospermum mopane, Coronillia varia,Cotoneaster serotina, Crataegus spp., Cucumis spp., Cupressus spp.,Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogonspp., Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davalliadivaricata, Desmodium spp., Dicksonia squarosa, Dibeteropogonamplectens, Dioclea spp, Dolichos spp., Dorvcnium rectum, Echinochloapyramidalis, Ehraffia spp., Eleusine coracana, Eragrestis spp.,Erythrina spp., Eucalypfus spp., Euclea schimperi, Eulalia vi/losa,Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingia spp,Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycinejavanica, Gliricidia spp, Gossypium hirsutum, Grevillea spp., Guibourtiacoleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus,Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffheliadissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia,Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex,Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihotesculenta, Medicago saliva, Metasequoia glyptostroboides, Musasapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryzaspp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petuniaspp., Phaseolus spp., Phoenix canariensis, Phormium cookianum, Photiniaspp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara,Pogonarthria fleckii, Pogonaffhria squarrosa, Populus spp., Prosopiscineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis,Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhusnatalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia, Rosaspp., Rubus spp., Salix spp., Schyzachyrium sanguineum, Sciadopitysvefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghumbicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides,Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themedatriandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vacciniumspp., Vicia spp., Vitis vinifera, Watsonia pyramidata, Zantedeschiaaethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brusselssprouts, cabbage, canola, carrot, cauliflower, celery, collard greens,flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean,straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize,wheat, barely, rye, oat, peanut, pea, lentil and alfalfa, cotton,rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, atree, an ornamental plant, a perennial grass and a forage crop.Alternatively algae and other non-Viridiplantae can be used for themethods of the present invention.

According to some embodiments of the invention, the plant used by themethod of the invention is a crop plant such as rice, maize, wheat,barley, peanut, potato, sesame, olive tree, palm oil, banana, soybean,sunflower, canola, sugarcane, alfalfa, millet, leguminosae (bean, pea),flax, lupinus, rapeseed, tobacco, poplar and cotton.

Expressing the exogenous polynucleotide of the invention within theplant can be effected by transforming one or more cells of the plantwith the exogenous polynucleotide, followed by generating a mature plantfrom the transformed cells and cultivating the mature plant underconditions suitable for expressing the exogenous polynucleotide withinthe mature plant.

According to some embodiments of the invention, the transformation iseffected by introducing to the plant cell a nucleic acid construct whichincludes the exogenous polynucleotide of some embodiments of theinvention and at least one promoter for directing transcription of theexogenous polynucleotide in a host cell (a plant cell). Further detailsof suitable transformation approaches are provided hereinbelow.

According to some embodiments of the invention, there is provided anucleic acid construct comprising the isolated polynucleotide of theinvention, and a promoter for directing transcription of the nucleicacid sequence in a host cell.

As used herein, the term “promoter” refers to a region of DNA which liesupstream of the transcriptional initiation site of a gene to which RNApolymerase binds to initiate transcription of RNA. The promoter controlswhere (e.g., which portion of a plant) and/or when (e.g., at which stageor condition in the lifetime of an organism) the gene is expressed.

Any suitable promoter sequence can be used by the nucleic acid constructof the present invention. Preferably the promoter is a constitutivepromoter, a tissue-specific, or an abiotic stress-inducible promoter.

Suitable constitutive promoters include, for example, CaMV 35S promoter(SEQ ID NO:777; Odell et al., Nature 313:810-812, 1985); ArabidopsisAt6669 promoter (SEQ ID NO:775; see PCT Publication No. WO04081173A2);maize Ubi 1 (Christensen et al., Plant Sol. Biol. 18:675-689, 1992);rice actin (McElroy et al., Plant Cell 2:163-171, 1990); pEMU (Last etal., Theor. Appl. Genet. 81:581-588, 1991); CaMV 19S (Nilsson et al.,Physiol. Plant 100:456-462, 1997); GOS2 (de Pater et al, Plant JNovember; 2(6):837-44, 1992); ubiquitin (Christensen et al, Plant Mol.Biol. 18: 675-689, 1992); Rice cyclophilin (Bucholz et al, Plant MolBiol. 25(5):837-43, 1994); Maize H3 histone (Lepetit et al, Mol. Gen.Genet. 231: 276-285, 1992); Actin 2 (An et al, Plant J. 10(1); 107-121,1996) and Synthetic Super MAS (Ni et al., The Plant Journal 7: 661-76,1995). Other constitutive promoters include those in U.S. Pat. Nos.5,659,026, 5,608,149; 5.608,144; 5,604,121; 5.569,597: 5.466,785;5,399,680; 5,268,463; and 5,608,142.

Suitable tissue-specific promoters include, but not limited to,leaf-specific promoters [such as described, for example, by Yamamoto etal., Plant J. 12:255-265, 1997; Kwon et al., Plant Physiol. 105:357-67,1994; Yamamoto et al., Plant Cell Physiol. 35:773-778, 1994; Gotor etal., Plant J. 3:509-18, 1993; Orozco et al., Plant Mol. Biol.23:1129-1138, 1993; and Matsuoka et al., Proc. Natl. Acad. Sci. USA90:9586-9590, 1993], seed-preferred promoters [e.g., from seed specificgenes (Simon, et al., Plant Mol. Biol. 5. 191, 1985; Scofield, et al.,J. Biol. Chem. 262: 12202, 1987; Baszczynski, et al., Plant Mol. Biol.14: 633, 1990), Brazil Nut albumin (Pearson et al., Plant Mol. Biol. 18:235-245, 1992), legumin (Ellis, et al. Plant Mol. Biol. 10: 203-214,1988), Glutelin (rice) (Takaiwa, et al., Mol. Gen. Genet. 208: 15-22,1986; Takaiwa, et al., FEBS Letts. 221: 43-47, 1987), Zein (Matzke et alPlant Mol Biol, 143). 323-32 1990), napA (Stalberg, et al, Planta 199:515-519, 1996), Wheat SPA (Albanietal, Plant Cell, 9: 171-184, 1997),sunflower oleosin (Cummins, et al., Plant Mol. Biol. 19: 873-876,1992)], endosperm specific promoters [e.g., wheat LMW and HMW,glutenin-1 (Mol Gen Genet 216:81-90, 1989; NAR 17:461-2), wheat a, b andg gliadins (EMBO3:1409-15, 1984), Barley ltrl promoter, barley B1, C, Dhordein (Theor Appl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; MolGen Genet 250:750-60, 1996), Barley DOF (Mena et al, The Plant Journal,116(1): 53-62, 1998), Biz2 (EP99106056.7), Synthetic promoter(Vicente-Carbajosa et al., Plant J. 13: 629-640, 1998), rice prolaminNRP33, rice-globulin Glb-1 (Wu et al, Plant Cell Physiology 39(8)885-889, 1998), rice alpha-globulin REB/OHP-1 (Nakase et al. Plant Mol.Biol. 33: 513-S22, 1997), rice ADP-glucose PP (Trans Res 6:157-68,1997), maize ESR gene family (Plant J 12:235-46, 1997), sorgumgamma-kafirin (PMB 32:1029-35, 1996)], embryo specific promoters [e.g.,rice OSH1 (Sato et al, Proc. Nati. Acad. Sci. USA, 93: 8117-8122), KNOX(Postma-Haarsma et al, Plant Mol. Biol. 39:257-71, 1999), rice oleosin(Wu et at, J. Biochem., 123:386, 1998)], and flower-specific promoters[e.g., AtPRP4, chalene synthase (chsA) (Van der Meer, et al., Plant Mol.Biol. 15, 95-109, 1990), LAT52 (Twell et al Mol. Gen Genet. 217:240-245;1989), apetala-3].

Suitable abiotic stress-inducible promoters include, but not limited to,salt-inducible promoters such as RD29A (Yamaguchi-Shinozalei et al.,Mol. Gen. Genet. 236:331-340, 1993); drought-inducible promoters such asmaize rab17 gene promoter (Pla et. al., Plant Mol. Biol. 21:259-266,1993), maize rab28 gene promoter (Busk et. al., Plant J. 11:1285-1295,1997) and maize Ivr2 gene promoter (Pelleschi et. al., Plant Mol. Biol.39:373-380, 1999); heat-inducible promoters such as heat tomatohsp80-promoter from tomato (U.S. Pat. No. 5,187,267).

As mentioned above, and further described in Example 6 of the Examplessection which follows, the present inventors have uncovered novelpromoter sequences (regulatory nucleic acid sequences) which can be usedto express a polynucleotide-of-interest in a plant.

Thus, according to an aspect of some embodiments of the invention, thereis provided a nucleic acid construct comprising a nucleic acid sequenceselected from the group consisting of SEQ ID NOs:779-792 and aheterologous polynucleotide sequence, wherein the nucleic acid sequenceis capable of regulating expression of the heterologous polynucleotidein a host cell.

According to some embodiments of the invention the heterologouspolynucleotide is operably linked to the regulatory nucleic acidsequence selected from the group consisting of SEQ ID NOs:779-792.

According to some embodiments of the invention, the regulatory nucleicacid sequence of the invention range in length from about 500nucleotides to about 4000 nucleotides and include one or more sequenceregions which are capable of recognizing and binding RNA polymerase IIand other proteins (trans-acting transcription factors) involved intranscription.

A coding nucleic acid sequence is “operably linked” to a regulatorysequence if the regulatory sequence is capable of exerting a regulatoryeffect on the coding sequence linked thereto. According to someembodiments of the invention, the regulatory sequence is positioned1-500 bp upstream of the ATG codon of the coding nucleic acid sequence,although it will be appreciated that regulatory sequences can also exerttheir effect when positioned elsewhere with respect to the codingnucleic acid sequence (e.g., within an intron).

As is clearly illustrated in the Examples section which follows, thenovel regulatory nucleic acid sequences of the invention are capable ofregulating expression of a coding nucleic acid sequence (e.g., areporter gene such as GUS, luciferase) operably linked thereto (seeExample 6 of the Examples section which follows).

According to some embodiments of the invention, the regulatory nucleicacid sequences of the invention regulate the expression of theheterologous polynucleotide in a tissue specific manner.

According to some embodiments of the invention, the regulatory nucleicacid sequences of the invention regulate the expression of theheterologous polynucleotide in a developmental stage—specific manner.

According to some embodiments of the invention, the regulatory nucleicacid sequences of the invention are modified to create variations in themolecule sequences such as to enhance their promoting activities, usingmethods known in the art, such as PCR-based DNA modification, orstandard DNA mutagenesis techniques, or by chemically synthesizing themodified polynucleotides.

Accordingly, the regulatory nucleic acid sequences set forth in SEQ IDNOs:779-792 may be truncated or deleted and still retain the capacity ofdirecting the transcription of an operably linked heterologous DNAsequence. The minimal length of a promoter region can be determined bysystematically removing sequences from the 5′ and 3′-ends of theisolated polynucleotide by standard techniques known in the art,including but not limited to removal of restriction enzyme fragments ordigestion with nucleases. Consequently, any sequence fragments,portions, or regions of the disclosed promoter polynucleotide sequencesof the invention can be used as regulatory sequences. It will beappreciated that modified sequences (mutated, truncated and the like)can acquire different transcriptional properties such as the directionof different pattern of gene expression as compared to the unmodifiedelement.

Optionally, the sequences set forth in SEQ ID NOs:779-792 may bemodified, for example for expression in a range of plant systems. Inanother approach, novel hybrid promoters can be designed or engineeredby a number of methods. Many promoters contain upstream sequences whichactivate, enhance or define the strength and/or specificity of thepromoter, such as described, for example, by Atchison [Ann. Rev. CellBiol. 4:127 (1988)]. T-DNA genes, for example contain “TATA” boxesdefining the site of transcription initiation and other upstreamelements located upstream of the transcription initiation site modulatetranscription levels [Gelvin In: Transgenic Plants (Kung, S.-D. and Us,R., Eds, San Diego: Academic Press, pp. 49-87, (1988)]. Another chimericpromoter combined a trimer of the octopine synthase (OCS) activator tothe mannopine synthase (mas) activator plus promoter and reported anincrease in expression of a reporter gene [Min Ni et al. The PlantJournal 7:661 (1995)]. The upstream regulatory sequences of the promoterpolynucleotide sequences of the invention can be used for theconstruction of such chimeric or hybrid promoters. Methods forconstruction of variant promoters include, but are not limited to,combining control elements of different promoters or duplicatingportions or regions of a promoter (see for example, U.S. Pat. Nos.5,110,732 and 5,097,025). Those of skill in the art are familiar withthe specific conditions and procedures for the construction,manipulation and isolation of macromolecules (e.g., DNA molecules,plasmids, etc.), generation of recombinant organisms and the screeningand isolation of genes, [see for example Sambrook et al., MolecularCloning: A Laboratory Manual, Cold Spring Harbor Press, (1989); Mailgaet al., Methods in Plant Molecular Biology, Cold Spring Harbor Press,(1995); Birren et al., Genome Analysis: volume 1, Analyzing DNA, (1997);volume 2, Detecting Genes, (1998); volume 3, Cloning Systems, (1999);and volume 4, Mapping Genomes, (1999), Cold Spring Harbor, N.Y].

According to some embodiments of the invention the heterologouspolynucleotide which is regulated by the regulatory nucleic acidsequence of the invention (e.g., SEQ ID NO:779-791 or 792) comprises anucleic acid sequence at least about 80%, at least about 81%, at leastabout 82%, at least about 83%, at least about 84%, at least about 85%,at least about 86%, at least about 87%, at least about 88%, at leastabout 89%, at least about 90%, at least about 91%, at least about 92%,at least about 93%, at least about 93%, at least about 94%, at leastabout 95%, at least about 96%, at least about 97%, at least about 98%,at least about 99%, e.g., 100% identical to the nucleic acid sequenceselected from the group consisting of SEQ ID NOs:18, 1-16, 19-50,101-378, 657-672, 674-706, 716-719, 724-741 and 755-763 with the provisothat the nucleic acid sequence is not as set forth by SEQ ID NO:756 or762.

According to some embodiments of the invention the heterologouspolynucleotide encodes an amino acid sequence at least about 80%, atleast about 81%, at least about 82%, at least about 83%, at least about84%, at least about 85%, at least about 86%, at least about 87%, atleast about 88%, at least about 89%, at least about 90%, at least about91%, at least about 92%, at least about 93%, at least about 93%, atleast about 94%, at least about 95%, at least about 96%, at least about97%, at least about 98%, at least about 99%, or more say 100% homologousto SEQ ID NO:68, 51-66, 69-100, 379-656, 707-715, 720-723, 742-754,764-771 or 772, with the proviso that the amino acid sequence is not asset forth by SEQ ID NO: 765 or 771.

According to some embodiments the heterologous polynucleotide does notencode the amino acid sequence set forth by SEQ ID NO: 809-851 or 852.

According to some embodiments the heterologous polynucleotide does notcomprise the nucleic acid sequence set forth by SEQ ID NO:807 or 808.

Thus, according to some embodiments of the invention, the method ofincreasing oil content, yield, growth rate, biomass, vigor, abioticstress tolerance and/or nitrogen use efficiency of a plant is effectedby expressing within the plant a nucleic acid construct of the inventionwhich comprises a nucleic acid sequence selected from the groupconsisting of SEQ ID NOs:779-792 and a heterologous polynucleotidesequence which comprises a nucleic acid sequence at least about 80%, atleast about 81%, at least about 82%, at least about 83%, at least about84%, at least about 85%, at least about 86%, at least about 87%, atleast about 88%, at least about 89%, at least about 90%, at least about91%, at least about 92%, at least about 93%, at least about 93%, atleast about 94%, at least about 95%, at least about 96%, at least about97%, at least about 98%, at least about 99%, e.g., 100% identical to SEQID NOs:18, 1-16, 19-50, 101-378, 657-672, 674-706, 716-719, 724-741 and755-763 with the proviso that the nucleic acid sequence is not as setforth by SEQ ID NO:756 or 762, wherein the nucleic acid sequence iscapable of regulating expression of the heterologous polynucleotide in ahost cell.

According to some embodiments of the invention, the method of increasingoil content, yield, growth rate, biomass, vigor, abiotic stresstolerance and/or nitrogen use efficiency of a plant is effected byexpressing within the plant a nucleic acid construct which comprises anucleic acid sequence selected from the group consisting of SEQ IDNOs:779-792 and a heterologous polynucleotide sequence which encodes anamino acid sequence at least about 80%, at least about 81%, at leastabout 82%, at least about 83%, at least about 84%, at least about 85%,at least about 86%, at least about 87%, at least about 88%, at leastabout 89%, at least about 90%, at least about 91%, at least about 92%,at least about 93%, at least about 93%, at least about 94%, at leastabout 95%, at least about 96%, at least about 97%, at least about 98%,at least about 99%, or more say 100% homologous to SEQ ID NO:68, 51-66,69-100, 379-656, 707-715, 720-723, 742-754, 764-771 or 772 with theproviso that the amino acid sequence is not as set forth by SEQ ID NO:765 or 771, wherein the nucleic acid sequence is capable of regulatingexpression of the heterologous polynucleotide in a host cell.

The nucleic acid construct of some embodiments of the invention canfurther include an appropriate selectable marker and/or an origin ofreplication. According to some embodiments of the invention, the nucleicacid construct utilized is a shuttle vector, which can propagate both inE. coli (wherein the construct comprises an appropriate selectablemarker and origin of replication) and be compatible with propagation incells. The construct according to the present invention can be, forexample, a plasmid, a bacemid, a phagemid, a cosmid, a phage, a virus oran artificial chromosome.

The nucleic acid construct of some embodiments of the invention can beutilized to stably or transiently transform plant cells. In stabletransformation, the exogenous polynucleotide is integrated into theplant genome and as such it represents a stable and inherited trait. Intransient transformation, the exogenous polynucleotide is expressed bythe cell transformed but it is not integrated into the genome and assuch it represents a transient trait.

There are various methods of introducing foreign genes into bothmonocotyledonous and dicotyledonous plants (Potrykus, I. Annu. Rev.Plant. Physiol., Plant. Mol. Biol. (1991) 42:205-225; Shimamoto et al.,Nature (1989) 338:274-276).

The principle methods of causing stable integration of exogenous DNAinto plant genomic DNA include two main approaches:

(i) Agrobacterium-mediated gene transfer: Klee et al. (1987) Annu. Rev.Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and SomaticCell Genetics of Plants, Vol. 6, Molecular Biology of Plant NuclearGenes, eds. Schell, J., and Vasil, L. K., Academic Publishers, SanDiego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds.Kung, S. and Arntzen, C. J., Butterworth Publishers, Boston, Mass.(1989) p. 93-112.

(ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and SomaticCell Genetics of Plants, Vol. 6, Molecular Biology of Plant NuclearGenes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego,Calif. (1989) p. 52-68; including methods for direct uptake of DNA intoprotoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNAuptake induced by brief electric shock of plant cells: Zhang et al.Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986)319:791-793. DNA injection into plant cells or tissues by particlebombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al.Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990)79:206-209; by the use of micropipette systems: Neuhaus et al., Theor.Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant.(1990) 79:213-217; glass fibers or silicon carbide whiskertransformation of cell cultures, embryos or callus tissue, U.S. Pat. No.5,464,765 or by the direct incubation of DNA with germinating pollen,DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman,G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p.197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719.

The Agrobacterium system includes the use of plasmid vectors thatcontain defined DNA segments that integrate into the plant genomic DNA.Methods of inoculation of the plant tissue vary depending upon the plantspecies and the Agrobacterium delivery system. A widely used approach isthe leaf disc procedure which can be performed with any tissue explantthat provides a good source for initiation of whole plantdifferentiation. See, e.g., Horsch et al. in Plant Molecular BiologyManual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. Asupplementary approach employs the Agrobacterium delivery system incombination with vacuum infiltration. The Agrobacterium system isespecially viable in the creation of transgenic dicotyledonous plants.

There are various methods of direct DNA transfer into plant cells. Inelectroporation, the protoplasts are briefly exposed to a strongelectric field. In microinjection, the DNA is mechanically injecteddirectly into the cells using very small micropipettes. In microparticlebombardment, the DNA is adsorbed on microprojectiles such as magnesiumsulfate crystals or tungsten particles, and the microprojectiles arephysically accelerated into cells or plant tissues.

Following stable transformation plant propagation is exercised. The mostcommon method of plant propagation is by seed. Regeneration by seedpropagation, however, has the deficiency that due to heterozygositythere is a lack of uniformity in the crop, since seeds are produced byplants according to the genetic variances governed by Mendelian rules.Basically, each seed is genetically different and each will grow withits own specific traits. Therefore, it is preferred that the transformedplant be produced such that the regenerated plant has the identicaltraits and characteristics of the parent transgenic plant. Therefore, itis preferred that the transformed plant be regenerated bymicropropagation which provides a rapid, consistent reproduction of thetransformed plants.

Micropropagation is a process of growing new generation plants from asingle piece of tissue that has been excised from a selected parentplant or cultivar. This process permits the mass reproduction of plantshaving the preferred tissue expressing the fusion protein. The newgeneration plants which are produced are genetically identical to, andhave all of the characteristics of, the original plant. Micropropagationallows mass production of quality plant material in a short period oftime and offers a rapid multiplication of selected cultivars in thepreservation of the characteristics of the original transgenic ortransformed plant. The advantages of cloning plants are the speed ofplant multiplication and the quality and uniformity of plants produced.

Micropropagation is a multi-stage procedure that requires alteration ofculture medium or growth conditions between stages. Thus, themicropropagation process involves four basic stages: Stage one, initialtissue culturing; stage two, tissue culture multiplication; stage three,differentiation and plant formation; and stage four, greenhouseculturing and hardening. During stage one, initial tissue culturing, thetissue culture is established and certified contaminant-free. Duringstage two, the initial tissue culture is multiplied until a sufficientnumber of tissue samples are produced to meet production goals. Duringstage three, the tissue samples grown in stage two are divided and growninto individual plantlets. At stage four, the transformed plantlets aretransferred to a greenhouse for hardening where the plants' tolerance tolight is gradually increased so that it can be grown in the naturalenvironment.

According to some embodiments of the invention, the transgenic plantsare generated by transient transformation of leaf cells, meristematiccells or the whole plant.

Transient transformation can be effected by any of the direct DNAtransfer methods described above or by viral infection using modifiedplant viruses.

Viruses that have been shown to be useful for the transformation ofplant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus(BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation ofplants using plant viruses is described in U.S. Pat. No. 4,855,237 (beangolden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese PublishedApplication No. 63-14693 (TMV), EPA 194,809 (BV), EPA 278,667 (BV); andGluzman, Y. et al., Communications in Molecular Biology: Viral Vectors,Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirusparticles for use in expressing foreign DNA in many hosts, includingplants are described in WO 87/06261.

According to some embodiments of the invention, the virus used fortransient transformations is avirulent and thus is incapable of causingsevere symptoms such as reduced growth rate, mosaic, ring spots, leafroll, yellowing, streaking, pox formation, tumor formation and pitting.A suitable avirulent virus may be a naturally occurring avirulent virusor an artificially attenuated virus. Virus attenuation may be effectedby using methods well known in the art including, but not limited to,sub-lethal heating, chemical treatment or by directed mutagenesistechniques such as described, for example, by Kurihara and Watanabe(Molecular Plant Pathology 4:259-269, 2003), Gal-on et al. (1992),Atreya et al. (1992) and Huet et al. (1994).

Suitable virus strains can be obtained from available sources such as,for example, the American Type Culture Collection (ATCC) or by isolationfrom infected plants. Isolation of viruses from infected plant tissuescan be effected by techniques well known in the art such as described,for example by Foster and Tatlor, Eds. “Plant Virology Protocols: FromVirus Isolation to Transgenic Resistance (Methods in Molecular Biology(Humana Pr), Vol 81)”, Humana Press, 1998. Briefly, tissues of aninfected plant believed to contain a high concentration of a suitablevirus, preferably young leaves and flower petals, are ground in a buffersolution (e.g., phosphate buffer solution) to produce a virus infectedsap which can be used in subsequent inoculations.

Construction of plant RNA viruses for the introduction and expression ofnon-viral exogenous polynucleotide sequences in plants is demonstratedby the above references as well as by Dawson, W. O. et al., Virology(1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French etal. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters (1990)269:73-76; and U.S. Pat. No. 5,316,931.

When the virus is a DNA virus, suitable modifications can be made to thevirus itself. Alternatively, the virus can first be cloned into abacterial plasmid for ease of constructing the desired viral vector withthe foreign DNA. The virus can then be excised from the plasmid. If thevirus is a DNA virus, a bacterial origin of replication can be attachedto the viral DNA, which is then replicated by the bacteria.Transcription and translation of this DNA will produce the coat proteinwhich will encapsidate the viral DNA. If the virus is an RNA virus, thevirus is generally cloned as a cDNA and inserted into a plasmid. Theplasmid is then used to make all of the constructions. The RNA virus isthen produced by transcribing the viral sequence of the plasmid andtranslation of the viral genes to produce the coat protein(s) whichencapsidate the viral RNA.

In one embodiment, a plant viral polynucleotide is provided in which thenative coat protein coding sequence has been deleted from a viralpolynucleotide, a non-native plant viral coat protein coding sequenceand a non-native promoter, preferably the subgenomic promoter of thenon-native coat protein coding sequence, capable of expression in theplant host, packaging of the recombinant plant viral polynucleotide, andensuring a systemic infection of the host by the recombinant plant viralpolynucleotide, has been inserted. Alternatively, the coat protein genemay be inactivated by insertion of the non-native polynucleotidesequence within it, such that a protein is produced. The recombinantplant viral polynucleotide may contain one or more additional non-nativesubgenomic promoters. Each non-native subgenomic promoter is capable oftranscribing or expressing adjacent genes or polynucleotide sequences inthe plant host and incapable of recombination with each other and withnative subgenomic promoters. Non-native (foreign) polynucleotidesequences may be inserted adjacent the native plant viral subgenomicpromoter or the native and a non-native plant viral subgenomic promotersif more than one polynucleotide sequence is included. The non-nativepolynucleotide sequences are transcribed or expressed in the host plantunder control of the subgenomic promoter to produce the desiredproducts.

In a second embodiment, a recombinant plant viral polynucleotide isprovided as in the first embodiment except that the native coat proteincoding sequence is placed adjacent one of the non-native coat proteinsubgenomic promoters instead of a non-native coat protein codingsequence.

In a third embodiment, a recombinant plant viral polynucleotide isprovided in which the native coat protein gene is adjacent itssubgenomic promoter and one or more non-native subgenomic promoters havebeen inserted into the viral polynucleotide. The inserted non-nativesubgenomic promoters are capable of transcribing or expressing adjacentgenes in a plant host and are incapable of recombination with each otherand with native subgenomic promoters. Non-native polynucleotidesequences may be inserted adjacent the non-native subgenomic plant viralpromoters such that the sequences are transcribed or expressed in thehost plant under control of the subgenomic promoters to produce thedesired product.

In a fourth embodiment, a recombinant plant viral polynucleotide isprovided as in the third embodiment except that the native coat proteincoding sequence is replaced by a non-native coat protein codingsequence.

The viral vectors are encapsidated by the coat proteins encoded by therecombinant plant viral polynucleotide to produce a recombinant plantvirus. The recombinant plant viral polynucleotide or recombinant plantvirus is used to infect appropriate host plants. The recombinant plantviral polynucleotide is capable of replication in the host, systemicspread in the host, and transcription or expression of foreign gene(s)(exogenous polynucleotide) in the host to produce the desired protein.

Techniques for inoculation of viruses to plants may be found in Fosterand Taylor, eds. “Plant Virology Protocols: From Virus Isolation toTransgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol81)”, Humana Press, 1998; Maramorosh and Koprowski, eds. “Methods inVirology” 7 vols, Academic Press, New York 1967-1984; Hill, S. A.“Methods in Plant Virology”, Blackwell, Oxford, 1984; Walkey, D. G. A.“Applied Plant Virology”, Wiley, New York, 1985; and Kado and Agrawa,eds. “Principles and Techniques in Plant Virology”, VanNostrand-Reinhold, New York.

In addition to the above, the polynucleotide of the present inventioncan also be introduced into a chloroplast genome thereby enablingchloroplast expression.

A technique for introducing exogenous polynucleotide sequences to thegenome of the chloroplasts is known. This technique involves thefollowing procedures. First, plant cells are chemically treated so as toreduce the number of chloroplasts per cell to about one. Then, theexogenous polynucleotide is introduced via particle bombardment into thecells with the aim of introducing at least one exogenous polynucleotidemolecule into the chloroplasts. The exogenous polynucleotides selectedsuch that it is integratable into the chloroplast's genome viahomologous recombination which is readily effected by enzymes inherentto the chloroplast. To this end, the exogenous polynucleotide includes,in addition to a gene of interest, at least one polynucleotide stretchwhich is derived from the chloroplast's genome. In addition, theexogenous polynucleotide includes a selectable marker, which serves bysequential selection procedures to ascertain that all or substantiallyall of the copies of the chloroplast genomes following such selectionwill include the exogenous polynucleotide. Further details relating tothis technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507which are incorporated herein by reference. A polypeptide can thus beproduced by the protein expression system of the chloroplast and becomeintegrated into the chloroplast's inner membrane.

Since processes which increase oil content, yield, growth rate, biomass,vigor, abiotic stress tolerance and/or nitrogen use efficiency of aplant can involve multiple genes acting additively or in synergy (see,for example, in Quesda et al., Plant Physiol. 130:951-063, 2002), thepresent invention also envisages expressing a plurality of exogenouspolynucleotides in a single host plant to thereby achieve superioreffect on oil content, yield, growth rate, biomass, vigor, abioticstress tolerance and/or nitrogen use efficiency.

Expressing a plurality of exogenous polynucleotides in a single hostplant can be effected by co-introducing multiple nucleic acidconstructs, each including a different exogenous polynucleotide, into asingle plant cell. The transformed cell can than be regenerated into amature plant using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in asingle host plant can be effected by co-introducing into a singleplant-cell a single nucleic-acid construct including a plurality ofdifferent exogenous polynucleotides. Such a construct can be designedwith a single promoter sequence which can transcribe a polycistronicmessenger RNA including all the different exogenous polynucleotidesequences. To enable co-translation of the different polypeptidesencoded by the polycistronic messenger RNA, the polynucleotide sequencescan be inter-linked via an internal ribosome entry site (IRES) sequencewhich facilitates translation of polynucleotide sequences positioneddownstream of the IRES sequence. In this case, a transcribedpolycistronic RNA molecule encoding the different polypeptides describedabove will be translated from both the capped 5′ end and the twointernal IRES sequences of the polycistronic RNA molecule to therebyproduce in the cell all different polypeptides. Alternatively, theconstruct can include several promoter sequences each linked to adifferent exogenous polynucleotide sequence.

The plant cell transformed with the construct including a plurality ofdifferent exogenous polynucleotides, can be regenerated into a matureplant, using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in asingle host plant can be effected by introducing different nucleic acidconstructs, including different exogenous polynucleotides, into aplurality of plants. The regenerated transformed plants can then becross-bred and resultant progeny selected for superior abiotic stresstolerance, growth, biomass, yield, vigor and/or nitrogen use efficiencytraits, using conventional plant breeding techniques.

As mentioned above, and further described in Example 9 of the Examplessection which follows, the present inventors have uncovered thatdownregulating the expression level of the BDL127 gene product (e.g.,the polynucleotides set forth by SEQ ID NO:17 or 673; or the polypeptideset forth by SEQ ID NO:67) and/or of homologous thereof can be used toincrease yield (e.g., seed yield), oil content, biomass, growth rate,vigor, ABST and/or NUE in a plant.

In some cases, overexpression of the exogenous polynucleotide within theplant can result in silencing of the endogenous polynucleotide (which ishomologous of the exogenous polynucleotide), probably through RNAinterference or co-suppression mechanisms. To test the effect ofdownregulation of the polynucleotide(s) of the invention on the desiredplant trait (e.g., plant yield, oil content, biomass, vigor, ABST orNUE), various downregulation methods and agents can be used.

Downregulation (gene silencing) of the transcription or translationproduct of an endogenous gene such as BDL127 can be achieved by methodswhich are well known in the art e.g., co-suppression, antisensesuppression, RNA interference and ribozyme polynucleotide molecules,changing the promoter structure, removal or creation transcriptionfactor binding sites or expression under different promoters. Guidelinesfor effecting same are provided infra.

Co-suppression (sense suppression)—Inhibition of the endogenous gene canbe achieved by co-suppression, using an RNA molecule (or an expressionvector encoding same) which is in the sense orientation with respect tothe transcription direction of the endogenous gene. The polynucleotideused for co-suppression may correspond to all or part of the sequenceencoding the endogenous polypeptide and/or to all or part of the 5′and/or 3′ untranslated region of the endogenous transcript; it may alsobe an unpolyadenylated RNA; an RNA which lacks a 5′ cap structure; or anRNA which contains an unsplicable intron. In some embodiments, thepolynucleotide used for co-suppression is designed to eliminate thestart codon of the endogenous polynucleotide so that no protein productwill be translated. However, as with antisense suppression, thesuppressive efficiency is enhanced as specificity of hybridization isincreased, e.g., as the introduced sequence is lengthened, and/or as thesequence similarity between the introduced sequence and the endogenousgene is increased. For further details see U.S. Pat. Appl. Nos.20050172364 and No. 5,231,020 which are fully incorporated herein byreference.

According to some embodiments of the invention, the exogenouspolynucleotide comprises an untranslatable nucleic acid sequence, e.g.,a sequence comprising one or more pre-mature stop codons, or nonsensemutations, such as described in U.S. Pat. No. 5,583,021.

According to some embodiments of the invention, downregulation of theendogenous gene is performed using an amplicon expression vector whichcomprises a plant virus-derived sequence that contains all or part ofthe target gene but generally not all of the genes of the native virus.The viral sequences present in the transcription product of theexpression vector allow the transcription product to direct its ownreplication. The transcripts produced by the amplicon may be eithersense or antisense relative to the target sequence [see for example,Angell and Baulcombe, (1997) EMBO J. 16:3675-3684; Angell and Baulcombe,(1999) Plant J. 20:357-362, and U.S. Pat. No. 6,646,805, each of whichis herein incorporated by reference].

According to some embodiments of the invention, the exogenouspolynucleotide comprises an untranslatable nucleic acid sequence, e.g.,a sequence comprising one or more pre-mature stop codons, or nonsensemutations, such as described in U.S. Pat. No. 5,583,021.

Antisense suppression—Antisense suppression can be performed using anantisense polynucleotide or an expression vector which is designed toexpress an RNA molecule complementary to all or part of the messengerRNA (mRNA) encoding the endogenous polypeptide and/or to all or part ofthe 5′ and/or 3′ untranslated region of the endogenous gene. Overexpression of the antisense RNA molecule can result in reducedexpression of the native (endogenous) gene. The antisense polynucleotidemay be fully complementary to the target sequence (i.e., 100% identicalto the complement of the target sequence) or partially complementary tothe target sequence (i.e., less than 100% identical, e.g., less than90%, less than 80% identical to the complement of the target sequence).Antisense suppression may be used to inhibit the expression of multipleproteins in the same plant (see e.g., U.S. Pat. No. 5,942,657). Inaddition, portions of the antisense nucleotides may be used to disruptthe expression of the target gene. Generally, sequences of at leastabout 50 nucleotides, at least about 100 nucleotides, at least about 200nucleotides, at least about 300, at least about 400, at least about 450,at least about 500, at least about 550, or greater may be used. Methodsof using antisense suppression to inhibit the expression of endogenousgenes in plants are described, for example, in Liu, et al., (2002) PlantPhysiol. 129:1732-1743 and U.S. Pat. Nos. 5,759,829 and 5,942,657, eachof which is herein incorporated by reference. Efficiency of antisensesuppression may be increased by including a poly-dT region in theexpression cassette at a position 3′ to the antisense sequence and 5′ ofthe polyadenylation signal [See, U.S. Patent Publication No.20020048814, herein incorporated by reference].

RNA interference—RNA interference can be achieved using apolynucleotide, which can anneal to itself and form a double strandedRNA having a stem-loop structure (also called hairpin structure), orusing two polynucleotides, which form a double stranded RNA.

For hairpin RNA (hpRNA) interference, the expression vector is designedto express an RNA molecule that hybridizes to itself to form a hairpinstructure that comprises a single-stranded loop region and a base-pairedstem.

In some embodiments of the invention, the base-paired stem region of thehpRNA molecule determines the specificity of the RNA interference. Inthis configuration, the sense sequence of the base-paired stem regionmay correspond to all or part of the endogenous mRNA to bedownregulated, or to a portion of a promoter sequence controllingexpression of the endogenous gene to be inhibited; and the antisensesequence of the base-paired stem region is fully or partiallycomplementary to the sense sequence. Such hpRNA molecules are highlyefficient at inhibiting the expression of endogenous genes, in a mannerwhich is inherited by subsequent generations of plants [See, e.g.,Chuang and Meyerowitz, (2000) Proc. Natl. Acad. Sci. USA 97:4985-4990;Stoutjesdijk, et al., (2002) Plant Physiol. 129:1723-1731; andWaterhouse and Helliwell, (2003) Nat. Rev. Genet. 4:29-38; Chuang andMeyerowitz, (2000) Proc. Natl. Acad. Sci. USA 97:4985-4990; Pandolfiniet al., BMC Biotechnology 3:7; Panstruga, et al., (2003) Mol. Biol. Rep.30:135-140; and U.S. Patent Publication No. 2003/0175965; each of whichis incorporated by reference].

According to some embodiments of the invention, the sense sequence ofthe base-paired stem is from about 10 nucleotides to about 2,500nucleotides in length, e.g., from about 10 nucleotides to about 500nucleotides, e.g., from about 15 nucleotides to about 300 nucleotides,e.g., from about 20 nucleotides to about 100 nucleotides, e.g., or fromabout 25 nucleotides to about 100 nucleotides.

According to some embodiments of the invention, the antisense sequenceof the base-paired stem may have a length that is shorter, the same as,or longer than the length of the corresponding sense sequence.

According to some embodiments of the invention, the loop portion of thehpRNA can be from about 10 nucleotides to about 500 nucleotides inlength, for example from about 15 nucleotides to about 100 nucleotides,from about 20 nucleotides to about 300 nucleotides or from about 25nucleotides to about 400 nucleotides in length.

According to some embodiments of the invention, the loop portion of thehpRNA can include an intron (ihpRNA), which is capable of being splicedin the host cell. The use of an intron minimizes the size of the loop inthe hairpin RNA molecule following splicing and thus increasesefficiency of the interference [See, for example, Smith, et al., (2000)Nature 407:319-320; Wesley, et al., (2001) Plant J. 27:581-590; Wang andWaterhouse, (2001) Curr. Opin. Plant Biol. 5:146-150; Helliwell andWaterhouse, (2003) Methods 30:289-295; Brummell, et al. (2003) Plant J.33:793-800; and U.S. Patent Publication No. 2003/0180945; WO 98/53083;WO 99/32619; WO 98/36083; WO 99/53050; US 20040214330; US 20030180945;U.S. Pat. No. 5,034,323; U.S. Pat. No. 6,452,067; U.S. Pat. No.6,777,588; U.S. Pat. No. 6,573,099 and U.S. Pat. No. 6,326,527; each ofwhich is herein incorporated by reference].

In some embodiments of the invention, the loop region of the hairpin RNAdetermines the specificity of the RNA interference to its targetendogenous RNA. In this configuration, the loop sequence corresponds toall or part of the endogenous messenger RNA of the target gene. See, forexample, WO 02/00904; Mette, et al., (2000) EMBO J 19:5194-5201; Matzke,et al., (2001) Curr. Opin. Genet. Devel. 11:221-227; Scheid, et al.,(2002) Proc. Natl. Acad. Sci., USA 99:13659-13662; Aufsaftz, et al.,(2002) Proc. Nat'l. Acad. Sci. 99(4):16499-16506; Sijen, et al., Curr.Biol. (2001) 11:436-440), each of which is incorporated herein byreference.

For double-stranded RNA (dsRNA) interference, the sense and antisenseRNA molecules can be expressed in the same cell from a single expressionvector (which comprises sequences of both strands) or from twoexpression vectors (each comprising the sequence of one of the strands).Methods for using dsRNA interference to inhibit the expression ofendogenous plant genes are described in Waterhouse, et al., (1998) Proc.Natl. Acad. Sci. USA 95:13959-13964; and WO 99/49029, WO 99/53050, WO99/61631, and WO 00/49035; each of which is herein incorporated byreference.

According to some embodiments of the invention, RNA interference iseffected using an expression vector designed to express an RNA moleculethat is modeled on an endogenous micro RNAs (miRNA) gene. Micro RNAs(miRNAs) are regulatory agents consisting of about 22 ribonucleotidesand highly efficient at inhibiting the expression of endogenous genes[Javier, et al., (2003) Nature 425:257-263]. The miRNA gene encodes anRNA that forms a hairpin structure containing a 22-nucleotide sequencethat is complementary to the endogenous target gene.

Ribozyme—Catalytic RNA molecules, ribozymes, are designed to cleaveparticular mRNA transcripts, thus preventing expression of their encodedpolypeptides. Ribozymes cleave mRNA at site-specific recognitionsequences. For example, “hammerhead ribozymes” (see, for example, U.S.Pat. No. 5,254,678) cleave mRNAs at locations dictated by flankingregions that form complementary base pairs with the target mRNA. Thesole requirement is that the target RNA contains a 5′-UG-3′ nucleotidesequence. Hammerhead ribozyme sequences can be embedded in a stable RNAsuch as a transfer RNA (tRNA) to increase cleavage efficiency in vivo[Perriman et al. (1995) Proc. Natl. Acad. Sci. USA, 92(13):6175-6179; deFeyter and Gaudron Methods in Molecular Biology, Vol. 74, Chapter 43,“Expressing Ribozymes in Plants”, Edited by Turner, P. C, Humana PressInc., Totowa, N.J.; U.S. Pat. No. 6,423,885]. RNA endoribonucleases suchas that found in Tetrahymena thermophila are also useful ribozymes (U.S.Pat. No. 4,987,071).

Plant lines transformed with any of the downregulating moleculesdescribed hereinabove are screened to identify those that show thegreatest inhibition of the endogenous polynucleotide orpolypeptide-of-interest, and thereby the increase of the desired planttrait (e.g., yield, oil content, growth rate, biomass, vigor, NUE and/orABST).

Thus, the invention encompasses plants exogenously expressing thepolynucleotide(s), the nucleic acid constructs and/or polypeptide(s) ofthe invention. Once expressed within the plant cell or the entire plant,the level of the polypeptide encoded by the exogenous polynucleotide canbe determined by methods well known in the art such as, activity assays,Western blots using antibodies capable of specifically binding thepolypeptide, Enzyme-Linked ImmunoSorbent Assay (ELISA),radio-immuno-assays (RIA), immunohistochemistry, immunocytochemistry,immunofluorescence and the like.

The level of an RNA molecule-of interest in the plant [e.g., the RNAtranscribed from the exogenous polynucleotide of the invention or theendogenous RNA which is targeted by the downregulating molecule of theinvention] can be determined using methods well known in the art such asNorthern blot analysis, reverse transcription polymerase chain reaction(RT-PCR) analysis (including quantitative, semi-quantitative orreal-time RT-PCR) and RNA-in situ hybridization.

The endogenous homolog of the exogenous polynucleotide or polypeptide ofthe invention, or a fragment of the endogenous homolog (e.g. introns oruntranslated regions) in the plant can be used as a marker for markerassisted selection (MAS), in which a marker is used for indirectselection of a genetic determinant or determinants of a trait ofinterest (e.g., biomass, growth rate, oil content, yield, abiotic stresstolerance and/or nitrogen use efficiency). These genes (DNA or RNAsequence) may contain or be linked to polymorphic sites or geneticmarkers on the genome such as restriction fragment length polymorphism(RFLP), microsatellites and single nucleotide polymorphism (SNP), DNAfingerprinting (DFP), amplified fragment length polymorphism (AFLP),expression level polymorphism, polymorphism of the encoded polypeptideand any other polymorphism at the DNA or RNA sequence.

Examples of marker assisted selections include, but are not limited to,selection for a morphological trait (e.g., a gene that affects form,coloration, male sterility or resistance such as the presence or absenceof awn, leaf sheath coloration, height, grain color, aroma of rice);selection for a biochemical trait (e.g., a gene that encodes a proteinthat can be extracted and observed; for example, isozymes and storageproteins); selection for a biological trait (e.g., pathogen races orinsect biotypes based on host pathogen or host parasite interaction canbe used as a marker since the genetic constitution of an organism canaffect its susceptibility to pathogens or parasites).

The polynucleotides and polypeptides described hereinabove can be usedin a wide range of economical plants, in a safe and cost effectivemanner.

Plant lines exogenously expressing the polynucleotide or the polypeptideof the invention are screened to identify those that show the greatestincrease of the desired plant trait.

The effect of the transgene (the exogenous polynucleotide encoding thepolypeptide of the invention) or the downregulating molecule (e.g.,RNA-interference molecule) on abiotic stress tolerance can be determinedusing known methods.

Abiotic stress tolerance—Transformed (i.e., expressing the transgene)and non-transformed (wild type) plants are exposed to an abiotic stresscondition, such as water deprivation, suboptimal temperature (lowtemperature, high temperature), nutrient deficiency, nutrient excess, asalt stress condition, osmotic stress, heavy metal toxicity,anaerobiosis, atmospheric pollution and UV irradiation.

Salinity tolerance assay—Transgenic plants with tolerance to high saltconcentrations are expected to exhibit better germination, seedlingvigor or growth in high salt. Salt stress can be effected in many wayssuch as, for example, by irrigating the plants with a hyperosmoticsolution, by cultivating the plants hydroponically in a hyperosmoticgrowth solution (e.g., Hoagland solution), or by culturing the plants ina hyperosmotic growth medium [e.g., 50% Murashige-Skoog medium (MSmedium)]. Since different plants vary considerably in their tolerance tosalinity, the salt concentration in the irrigation water, growthsolution, or growth medium can be adjusted according to the specificcharacteristics of the specific plant cultivar or variety, so as toinflict a mild or moderate effect on the physiology and/or morphology ofthe plants (for guidelines as to appropriate concentration see,Bernstein and Kafkafi, Root Growth Under Salinity Stress In: PlantRoots, The Hidden Half 3rd ed. Waisel Y, Eshel A and Kafkafi U.(editors) Marcel Dekker Inc., New York, 2002, and reference therein).

For example, a salinity tolerance test can be performed by irrigatingplants at different developmental stages with increasing concentrationsof sodium chloride (for example 50 mM, 100 mM, 200 mM, 400 mM NaCl)applied from the bottom and from above to ensure even dispersal of salt.Following exposure to the stress condition the plants are frequentlymonitored until substantial physiological and/or morphological effectsappear in wild type plants. Thus, the external phenotypic appearance,degree of wilting and overall success to reach maturity and yieldprogeny are compared between control and transgenic plants. Quantitativeparameters of tolerance measured include, but are not limited to, theaverage wet and dry weight, the weight of the seeds yielded, the averageseed size and the number of seeds produced per plant. Transformed plantsnot exhibiting substantial physiological and/or morphological effects,or exhibiting higher biomass than wild-type plants, are identified asabiotic stress tolerant plants.

Osmotic tolerance test—Osmotic stress assays (including sodium chlorideand mannitol assays) are conducted to determine if an osmotic stressphenotype was sodium chloride-specific or if it was a general osmoticstress related phenotype. Plants which are tolerant to osmotic stressmay have more tolerance to drought and/or freezing. For salt and osmoticstress germination experiments, the medium is supplemented for examplewith 50 mM, 100 mM, 200 mM NaCl or 100 mM, 200 mM NaCl, 400 mM mannitol.

Drought tolerance assay/osmoticum assay—Tolerance to drought isperformed to identify the genes conferring better plant survival afteracute water deprivation. To analyze whether the transgenic plants aremore tolerant to drought, an osmotic stress produced by the presence ofsorbitol or polyethylene glycol (PEG 8000) in the medium is performed.Control and transgenic plants are germinated and grown in plant-agarplates for 10 days, after which they are transferred to platescontaining 1.5% PEG8000 or 500 mM of sorbitol. Plants are grown forabout additional 10 days. The treatment causes growth retardation, thenboth control and transgenic plants are compared, by measuring plantweight (fresh and dry), yield, and by growth rates.

Conversely, soil-based drought screens are performed with plantsoverexpressing the polynucleotides detailed above. Seeds from controlArabidopsis plants, or other transgenic plants overexpressing thepolypeptide of the invention are germinated and transferred to pots.Drought stress is obtained after irrigation is ceased accompanied byplacing the pots on absorbent paper to enhance the soil-drying rate.Transgenic and control plants are compared to each other when themajority of the control plants develop severe wilting. Plants arere-watered after obtaining a significant fraction of the control plantsdisplaying a severe wilting. Plants are ranked comparing to controls foreach of two criteria: tolerance to the drought conditions and recovery(survival) following re-watering.

Cold stress tolerance—To analyze cold stress, mature (25 day old) plantsare transferred to 4° C. chambers for 1 or 2 weeks, with constitutivelight. Later on plants are moved back to greenhouse. Two weeks laterdamages from chilling period, resulting in growth retardation and otherphenotypes, are compared between both control and transgenic plants, bymeasuring plant weight (wet and dry), and by comparing growth ratesmeasured as time to flowering, plant size, yield, and the like.

Heat stress tolerance—Heat stress tolerance is achieved by exposing theplants to temperatures above 34° C. for a certain period. Planttolerance is examined after transferring the plants back to 22° C. forrecovery and evaluation after 5 days relative to internal controls(non-transgenic plants) or plants not exposed to neither cold or heatstress.

Germination tests—Germination tests compare the percentage of seeds fromtransgenic plants that could complete the germination process to thepercentage of seeds from control plants that are treated in the samemanner. Normal conditions are considered for example, incubations at 22°C. under 22-hour light 2-hour dark daily cycles. Evaluation ofgermination and seedling vigor is conducted between 4 and 14 days afterplanting. The basal media is 50% MS medium (Murashige and Skoog, 1962Plant Physiology 15, 473-497).

Germination is checked also at unfavorable conditions such as cold(incubating at temperatures lower than 10° C. instead of 22° C.) orusing seed inhibition solutions that contain high concentrations of anosmolyte such as sorbitol (at concentrations of 50 mM, 100 mM, 200 mM,300 mM, 500 mM, and up to 1000 mM) or applying increasing concentrationsof salt (of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM NaCl).

The effect of the transgene (the exogenous polynucleotide encoding thepolypeptide of the invention) or the downregulating molecule (e.g.,RNA-interference molecule) on nitrogen use efficiency can be determinedusing known methods.

Nitrogen use efficiency assay using plantlets—Briefly, transgenic plantswhich are grown for 7-10 days in 0.5×MS [Murashige-Skoog] supplementedwith a selection agent are transferred to two nitrogen fertilizationconditions: MS media in which the combined nitrogen concentration(NH₄NO₃ and KNO₃) was 0.75 mM (nitrogen deficiency). Usually, 20randomly selected plants from each event of a gene are transferred tothe media. Plants are allowed to grow for additional 10 days. At the endof the 10 days plants are removed from the plate and immediately weighed(fresh weight) and then dried for 24 and re-weight (dry weight for laterstatistical analysis). Transgenic plants are compared to control plantsgrown in parallel under the same conditions. Mock-transgenic plants usedas control can be those transgenic plants expressing the uidA reportergene (GUS) under the same promoter or transgenic plants harboring onlythe same promoter but lacking any reporter gene.

Grain protein concentration—Grain protein content (g grain protein m⁻²)is estimated as the product of the mass of grain N (g grain N m⁻²)multiplied by the N/protein conversion ratio of k-5.13 (Mosse 1990,supra). The grain protein concentration is estimated as the ratio ofgrain protein content per unit mass of the grain (g grain protein kg⁻¹grain).

The effect of the transgene (the exogenous polynucleotide encoding thepolypeptide of the invention) or the downregulating molecule (e.g.,RNA-interference molecule) on plant's vigor, growth rate, biomass, yieldand/or oil content can be determined using known methods.

Plant vigor—The plant vigor can be calculated by the increase in growthparameters such as leaf area, fiber length, rosette diameter, plantfresh weight and the like per time.

Growth rate—The growth rate can be measured using digital analysis ofgrowing plants. For example, images of plants growing in greenhouse onplot basis can be captured every 3 days and the rosette area can becalculated by digital analysis. Rosette area growth is calculated usingthe difference of rosette area between days of sampling divided by thedifference in days between samples.

Evaluation of growth rate can be done by measuring plant biomassproduced, rosette area, leaf size or root length per time (can bemeasured in cm² per day of leaf area).

Relative growth area can be calculated using Formula I.Relative growth area rate=(ΔArea/Δt)*(1/Areat0)  Formula I

Δt is the current analyzed image day subtracted from the initial day(t−t0).

Thus, the relative growth area rate is in units of 1/day and lengthgrowth rate is in units of 1/day.

Seed yield—Evaluation of the seed yield per plant can be done bymeasuring the amount (weight or size) or quantity (i.e., number) of dryseeds produced and harvested from 8-16 plants and divided by the numberof plants.

For example, the total seeds from 8-16 plants can be collected, weightedusing e.g., an analytical balance and the total weight can be divided bythe number of plants.

Seed yield per growing area can be calculated in the same manner whiletaking into account the growing area given to a single plant. Increaseseed yield per growing area could be achieved by increasing seed yieldper plant, and/or by increasing number of plants capable of growing in agiven area.

In addition, seed yield can be determined via the weight of 1000 seeds.The weight of 1000 seeds can be determined as follows: seeds arescattered on a glass tray and a picture is taken. Each sample isweighted and then using the digital analysis, the number of seeds ineach sample is calculated.

The 1000 seeds weight can be calculated using formula II:1000 Seed Weight=number of seed in sample/sample weight×1000  Formula II

The Harvest Index can be calculated using Formula IIIHarvest Index=Average seed yield per plant/Average dry weight  FormulaIII

Fiber length—Fiber length can be measured using fibrograph. Thefibrograph system was used to compute length in terms of “Upper HalfMean” length. The upper half mean (UHM) is the average length of longerhalf of the fiber distribution. The fibrograph measures length in spanlengths at a given percentage point (cottoninc (dot)com/ClassificationofCotton/?Pg=4#Length).

Oil content—The oil content of a plant can be determined by extractionof the oil from the seed or the vegetative portion of the plant.Briefly, lipids (oil) can be removed from the plant (e.g., seed) bygrinding the plant tissue in the presence of specific solvents (e.g.,hexane or petroleum ether) and extracting the oil in a continuousextractor. Indirect oil content analysis can be carried out usingvarious known methods such as Nuclear Magnetic Resonance (NMR)Spectroscopy, which measures the resonance energy absorbed by hydrogenatoms in the liquid state of the sample [See for example, Conway T F.and Earle F R., 1963, Journal of the American Oil Chemists' Society;Springer Berlin/Heidelberg, ISSN: 0003-021X (Print) 1558-9331 (Online)];the Near Infrared (NI) Spectroscopy, which utilizes the absorption ofnear infrared energy (1100-2500 nm) by the sample; and a methoddescribed in WO/2001/023884, which is based on extracting oil a solvent,evaporating the solvent in a gas stream which forms oil particles, anddirecting a light into the gas stream and oil particles which forms adetectable reflected light.

Thus, the present invention is of high agricultural value for promotingthe yield of commercially desired crops (e.g., biomass of vegetativeorgan such as poplar wood, or reproductive organ such as number of seedsor seed biomass).

Any of the transgenic plants described hereinabove or parts thereof maybe processed to produce a feed, meal, protein or oil preparation, suchas for ruminant animals.

The transgenic plants described hereinabove, which exhibit increased oilcontent can be used to produce plant oil (by extracting the oil from theplant).

The plant oil (including the seed oil and/or the vegetative portion oil)produced according to the method of the invention may be combined with avariety of other ingredients. The specific ingredients included in aproduct are determined according to the intended use. Exemplary productsinclude animal feed, raw material for chemical modification,biodegradable plastic, blended food product, edible oil, biofuel,cooking oil, lubricant, biodiesel, snack food, cosmetics, andfermentation process raw material. Exemplary products to be incorporatedto the plant oil include animal feeds, human food products such asextruded snack foods, breads, as a food binding agent, aquaculturefeeds, fermentable mixtures, food supplements, sport drinks, nutritionalfood bars, multi-vitamin supplements, diet drinks, and cereal foods.According to some embodiments of the invention, the oil comprises a seedoil.

According to some embodiments of the invention, the oil comprises avegetative portion oil.

According to some embodiments of the invention, the plant cell forms apart of a plant.

As used herein the term “about” refers to ±10%.

The terms “comprises”, “comprising”, “includes”, “including”, “having”and their conjugates mean “including but not limited to”.

The term “consisting of means “including and limited to”.

The term “consisting essentially of” means that the composition, methodor structure may include additional ingredients, steps and/or parts, butonly if the additional ingredients, steps and/or parts do not materiallyalter the basic and novel characteristics of the claimed composition,method or structure.

As used herein, the singular form “a”, “an” and “the” include pluralreferences unless the context clearly dictates otherwise. For example,the term “a compound” or “at least one compound” may include a pluralityof compounds, including mixtures thereof.

Throughout this application, various embodiments of this invention maybe presented in a range format. It should be understood that thedescription in range format is merely for convenience and brevity andshould not be construed as an inflexible limitation on the scope of theinvention. Accordingly, the description of a range should be consideredto have specifically disclosed all the possible sub-ranges as well asindividual numerical values within that range. For example, descriptionof a range such as from 1 to 6 should be considered to have specificallydisclosed sub-ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numberswithin that range, for example, 1, 2, 3, 4, 5, and 6. This appliesregardless of the breadth of the range.

Whenever a numerical range is indicated herein, it is meant to includeany cited numeral (fractional or integral) within the indicated range.The phrases “ranging/ranges between” a first indicate number and asecond indicate number and “ranging/ranges from” a first indicate number“to” a second indicate number are used herein interchangeably and aremeant to include the first and second indicated numbers and all thefractional and integral numerals therebetween.

As used herein the term “method” refers to manners, means, techniquesand procedures for accomplishing a given task including, but not limitedto, those manners, means, techniques and procedures either known to, orreadily developed from known manners, means, techniques and proceduresby practitioners of the chemical, pharmacological, biological,biochemical and medical arts.

It is appreciated that certain features of the invention, which are, forclarity, described in the context of separate embodiments, may also beprovided in combination in a single embodiment. Conversely, variousfeatures of the invention, which are, for brevity, described in thecontext of a single embodiment, may also be provided separately or inany suitable subcombination or as suitable in any other describedembodiment of the invention. Certain features described in the contextof various embodiments are not to be considered essential features ofthose embodiments, unless the embodiment is inoperative without thoseelements.

Various embodiments and aspects of the present invention as delineatedhereinabove and as claimed in the claims section below find experimentalsupport in the following examples.

Unless otherwise defined, all technical and/or scientific terms usedherein have the same meaning as commonly understood by one of ordinaryskill in the art to which the invention pertains. Although methods andmaterials similar or equivalent to those described herein can be used inthe practice or testing of embodiments of the invention, exemplarymethods and/or materials are described below. In case of conflict, thepatent specification, including definitions, will control. In addition,the materials, methods, and examples are illustrative only and are notintended to be necessarily limiting.

EXAMPLES

Reference is now made to the following examples, which together with theabove descriptions illustrate some embodiments of the invention in a nonlimiting fashion.

Generally, the nomenclature used herein and the laboratory proceduresutilized in the present invention include molecular, biochemical,microbiological and recombinant DNA techniques. Such techniques arethoroughly explained in the literature. See, for example, “MolecularCloning: A laboratory Manual” Sambrook et al., (1989); “CurrentProtocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed.(1994); Ausubel et al., “Current Protocols in Molecular Biology”, JohnWiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide toMolecular Cloning”, John Wiley & Sons, New York (1988); Watson et al.,“Recombinant DNA”, Scientific American Books, New York; Birren et al.(eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, ColdSpring Harbor Laboratory Press, New York (1998); methodologies as setforth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis,J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-IIIColigan J. E., ed. (1994); Stites et al. (eds), “Basic and ClinicalImmunology” (8th Edition), Appleton & Lange, Norwalk, Conn. (1994);Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W.H. Freeman and Co., New York (1980); available immunoassays areextensively described in the patent and scientific literature, see, forexample, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578;3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533;3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521;“Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic AcidHybridization” Hames, B. D., and Higgins S. J., eds. (1985);“Transcription and Translation” Hames, B. D., and Higgins S. J., Eds.(1984); “Animal Cell Culture” Freshney, R. I., ed. (1986); “ImmobilizedCells and Enzymes” IRL Press, (1986); “A Practical Guide to MolecularCloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317,Academic Press; “PCR Protocols: A Guide To Methods And Applications”,Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategiesfor Protein Purification and Characterization—A Laboratory CourseManual” CSHL Press (1996); all of which are incorporated by reference asif fully set forth herein. Other general references are providedthroughout this document. The procedures therein are believed to be wellknown in the art and are provided for the convenience of the reader. Allthe information contained therein is incorporated herein by reference.

Example 1 Gene Identification and Gene Role Prediction UsingBioinformatics Tools

The present inventors have identified polynucleotides which can increaseplant yield, seed yield, oil yield, oil content, biomass, growth rate,abiotic stress tolerance, nitrogen use efficiency and/or vigor of aplant, as follows.

The nucleotide sequence datasets used here were from publicly availabledatabases or from sequences obtained using the Solexa technology (e.g.Barley and Sorghum). Sequence data from 100 different plant species wasintroduced into a single, comprehensive database. Other information ongene expression, protein annotation, enzymes and pathways were alsoincorporated. Major databases used include:

Genomes

Arabidopsis genome [TAIR genome version 6 (arabidopsis (dot) org/)];

Rice genome [IRGSP build 4.0 (rgp (dot) dna (dot) affrc (dot) go (dot)jp/IRGSP/)];

Poplar [Populus trichocarpa release 1.1 from JGI (assembly release v1.0)(genome (dot) jgi-psf (dot) org/)];

Brachypodium [JGI 4× assembly, brachpodium (dot) org)];

Soybean [DOE-JGI SCP, version Glyma0 (phytozome (dot) net/)];

Grape [French-Italian Public Consortium for Grapevine GenomeCharacterization grapevine genome (genoscope (dot) cns (dot) fr/)];

Castorbean [TIGR/J Craig Venter Institute 4× assembly [jcvi (dot)org/r_communis];

Sorghum [DOE-JGI SCP, version Sbi1 [phytozome (dot) net/)];

Partially assembled genome of Maize [maizesequence (dot) org/];

Expressed EST and mRNA Sequences were Extracted from the FollowingDatabases:

GenBank versions 154, 157, 160, 161, 164, 165, 166 and 168 (ncbi (dot)nlm (dot) nih (dot) gov/dbEST/);

RefSeq (ncbi (dot) nlm (dot) nih (dot) gov/RefSeq/);

TAIR (arabidopsis (dot) org/);

Protein and Pathway Databases

Uniprot [uniprot (dot) org/].

AraCyc [arabidopsis (dot) org/biocyc/index (dot) jsp].

ENZYME [expasy (dot) org/enzyme/].

Microarray Datasets were Downloaded from:

GEO (ncbi (dot) nlm (dot) nih (dot) gov/geo/)

TAIR (arabidopsis (dot) org/).

Proprietary Evogene's microarray data (See WO2008/122980 to Evogene andExample 3 below.

QTL and SNPs Information

Gramene [gramene (dot) org/qtl/].

Panzea [panzea (dot) org/index (dot) html].

Database Assembly—was performed to build a wide, rich, reliableannotated and easy to analyze database comprised of publicly availablegenomic mRNA, ESTs DNA sequences, data from various crops as well asgene expression, protein annotation and pathway data QTLs, and otherrelevant information.

Database assembly is comprised of a toolbox of gene refining,structuring, annotation and analysis tools enabling to construct atailored database for each gene discovery project. Gene refining andstructuring tools enable to reliably detect splice variants andantisense transcripts, generating understanding of various potentialphenotypic outcomes of a single gene. The capabilities of the “LEADS”platform of Compugen LTD for analyzing human genome have been confirmedand accepted by the scientific community [see e.g., “WidespreadAntisense Transcription”, Yelin, et al. (2003) Nature Biotechnology 21,379-85; “Splicing of Alu Sequences”, Lev-Maor, et al. (2003) Science 300(5623), 1288-91; “Computational analysis of alternative splicing usingEST tissue information”, Xie H et al. Genomics 2002], and have beenproven most efficient in plant genomics as well.

EST clustering and gene assembly—For gene clustering and assembly oforganisms with available genome sequence data (arabidopsis, rice,castorbean, grape, brachypodium, poplar, soybean, sorghum) the genomicLEADS version (GANG) was employed. This tool allows most accurateclustering of ESTs and mRNA sequences on genome, and predicts genestructure as well as alternative splicing events and anti-sensetranscription.

For organisms with no available full genome sequence data, “expressedLEADS” clustering software was applied.

Gene annotation—Predicted genes and proteins were annotated as follows:Blast search [blast (dot) ncbi (dot) nlm (dot) nih (dot) gov/Blast (dot)cgi] against all plant UniProt [uniprot (dot) org/] sequences wasperformed. Open reading frames of each putative transcript were analyzedand longest ORF with higher number of homologues was selected aspredicted protein of the transcript. The predicted proteins wereanalyzed by InterPro [ebi (dot) ac (dot) uk/interpro/].

Blast against proteins from AraCyc and ENZYME databases was used to mapthe predicted transcripts to AraCyc pathways.

Predicted proteins from different species were compared using blastalgorithm [ncbi (dot) nlm (dot) nih (dot) gov/Blast (dot) cgi] tovalidate the accuracy of the predicted protein sequence, and forefficient detection of orthologs.

Gene expression profiling—Several data sources were exploited for geneexpression profiling which combined microarray data and digitalexpression profile (see below). According to gene expression profile, acorrelation analysis was performed to identify genes which areco-regulated under different developmental stages and environmentalconditions and which are associated with different phenotypes.

Publicly available microarray datasets were downloaded from TAIR andNCBI GEO sites, renormalized, and integrated into the database.Expression profiling is one of the most important resource data foridentifying genes important for yield.

A digital expression profile summary was compiled for each clusteraccording to all keywords included in the sequence records comprisingthe cluster. Digital expression, also known as electronic Northern Blot,is a tool that displays virtual expression profile based on the ESTsequences forming the gene cluster. The tool provides the expressionprofile of a cluster in terms of plant anatomy (e.g., the tissue/organin which the gene is expressed), developmental stage (the developmentalstages at which a gene can be found) and profile of treatment (providesthe physiological conditions under which a gene is expressed such asdrought, cold, pathogen infection, etc). Given a random distribution ofESTs in the different clusters, the digital expression provides aprobability value that describes the probability of a cluster having atotal of N ESTs to contain X ESTs from a certain collection oflibraries. For the probability calculations, the following is taken intoconsideration: a) the number of ESTs in the cluster, b) the number ofESTs of the implicated and related libraries, c) the overall number ofESTs available representing the species. Thereby clusters with lowprobability values are highly enriched with ESTs from the group oflibraries of interest indicating a specialized expression.

Recently, the accuracy of this system was demonstrated by Portnoy etal., 2009 (Analysis Of The Melon Fruit Transcriptome Based On 454Pyrosequencing) in: Plant & Animal Genomes XVII Conference, San Diego,Calif. Transcriptomic analysis, based on relative EST abundance in datawas performed by 454 pyrosequencing of cDNA representing mRNA of themelon fruit. Fourteen double strand cDNA samples obtained from twogenotypes, two fruit tissues (flesh and rind) and four developmentalstages were sequenced. GS FLX pyrosequencing (Roche/454 Life Sciences)of non-normalized and purified cDNA samples yielded 1,150,657 expressedsequence tags that assembled into 67,477 unigenes (32,357 singletons and35,120 contigs). Analysis of the data obtained against the CucurbitGenomics Database [icugi (dot) org/] confirmed the accuracy of thesequencing and assembly. Expression patterns of selected genes fittedwell their qRT-PCR data.

The concepts of orthology and paralogy have recently been applied tofunctional characterizations and classifications on the scale ofwhole-genome comparisons. Orthologs and paralogs constitute two majortypes of homologs: The first evolved from a common ancestor byspecialization, and the latter are related by duplication events. It isassumed that paralogs arising from ancient duplication events are likelyto have diverged in function while true orthologs are more likely toretain identical function over evolutionary time.

To identify putative orthologs of the genes affecting plant yield, oilyield, oil content, seed yield, growth rate, vigor, biomass, abioticstress tolerance and/or nitrogen use efficiency, all sequences werealigned using the BLAST (Basic Local Alignment Search Tool). Sequencessufficiently similar were tentatively grouped. These putative orthologswere further organized under a Phylogram—a branching diagram (tree)assumed to be a representation of the evolutionary relationships amongthe biological taxa. Putative ortholog groups were analyzed as to theiragreement with the phylogram and in cases of disagreements theseortholog groups were broken accordingly. Expression data was analyzedand the EST libraries were classified using a fixed vocabulary of customterms such as developmental stages (e.g., genes showing similarexpression profile through development with up regulation at specificstage, such as at the seed filling stage) and/or plant organ (e.g.,genes showing similar expression profile across their organs with upregulation at specific organs such as seed). The annotations from allthe ESTs clustered to a gene were analyzed statistically by comparingtheir frequency in the cluster versus their abundance in the database,allowing the construction of a numeric and graphic expression profile ofthat gene, which is termed “digital expression”. The rationale of usingthese two complementary methods with methods of phenotypic associationstudies of QTLs, SNPs and phenotype expression correlation is based onthe assumption that true orthologs are likely to retain identicalfunction over evolutionary time. These methods provide different sets ofindications on function similarities between two homologous genes,similarities in the sequence level—identical amino acids in the proteindomains and similarity in expression profiles.

Overall, 50 genes were identified to have a major impact on plant yield,seed yield, oil yield, oil content, biomass, growth rate, vigor, abioticstress tolerance (ABST) and/or nitrogen use efficiency (NUE) whenexpressed in a plant. The identified genes, their curated polynucleotideand polypeptide sequences, as well as their updated sequences accordingto Genbank database are provided in Table 1, hereinbelow.

TABLE 1 Identified polynucleotides which affect plant yield, seed yield,oil yield, oil content, biomass, growth rate, vigor, abiotic stresstolerance and/or nitrogen use efficiency of a plant PolynucleotidePolypeptide Gene Name Cluster Name Organism SEQ ID NO: SEQ ID NO: BDL100rice|gb157.2|BI812936 rice 1 51 BDL100 1 723 BDL106canola|gb161|DY020650 canola 2 52 BDL106 724 52 BDL108canola|gb161|CD818601 canola 3 53 BDL108 725 742 BDL108 726 743 BDL110canola|gb161|CD814521 canola 4 54 BDL110 727 744 BDL110 728 745 BDL111canola|gb161|CN829852 canola 5 55 BDL111 729 746 BDL111 730 747 BDL112arabidopsis|gb165|AT3G23510 arabidopsis 6 56 BDL113arabidopsis|gb165|AT2G45310 arabidopsis 7 57 BDL114arabidopsis|gb165|AT5G27820 arabidopsis 8 58 BDL115arabidopsis|gb165|AT4G11090 arabidopsis 9 59 BDL116arabidopsis|gb165|AT4G24175 arabidopsis 10 60 BDL119arabidopsis|gb165|AT3G47965 arabidopsis 11 61 BDL119 731 748 BDL120arabidopsis|gb165|AT3G03230 arabidopsis 12 62 BDL120 732 749 BDL122arabidopsis|gb165|AT3G49000 arabidopsis 13 63 BDL123arabidopsis|gb165|AT2G21860 arabidopsis 14 64 BDL124arabidopsis|gb165|AT5G51590 arabidopsis 15 65 BDL124 733 750 BDL125arabidopsis|gb165|AT3G16180 arabidopsis 16 66 BDL125 734 751 BDL127arabidopsis|gb165|AT3G44940 arabidopsis 17 67 BDL128arabidopsis|gb165|AT1G60770 arabidopsis 18 68 BDL129arabidopsis|gb165|AT4G08690 arabidopsis 19 69 BDL129 735 69 BDL130arabidopsis|gb165|AT3G03870 arabidopsis 20 70 BDL130 736 752 BDL131arabidopsis|gb165|AT4G27450 arabidopsis 21 71 BDL131 737 71 BDL132arabidopsis|gb165|AT4G23730 arabidopsis 22 72 BDL133arabidopsis|gb165|AT3G06150 arabidopsis 23 73 BDL134arabidopsis|gb165|AT3G28420 arabidopsis 24 74 BDL134 738 753 BDL135arabidopsis|gb165|AT3G18600 arabidopsis 25 75 BDL136arabidopsis|gb165|AT3G22990 arabidopsis 26 76 BDL137arabidopsis|gb165|AT5G14530 arabidopsis 27 77 BDL139arabidopsis|gb165|AT1G29800 arabidopsis 28 78 BDL141arabidopsis|gb165|AT1G29980 arabidopsis 29 79 BDL142arabidopsis|gb165|AT2G39110 arabidopsis 30 80 BDL142 30 754 BDL143arabidopsis|gb165|AT1G62810 arabidopsis 31 81 BDL143 739 81 BDL144arabidopsis|gb165|AT3G14890 arabidopsis 32 82 BDL145arabidopsis|gb165|AT1G24470 arabidopsis 33 83 BDL145 740 83 BDL146arabidopsis|gb165|AT3G09310 arabidopsis 34 84 BDL146 741 84 BDL148arabidopsis|gb165|AT4G35785 arabidopsis 35 85 BDL42arabidopsis|gb165|AT5G13170 arabidopsis 36 86 BDL46arabidopsis|gb165|AT2G13690 arabidopsis 37 87 BDL46 719 87 BDL51arabidopsis|gb165|AT5G64260 arabidopsis 38 88 BDL52tomato|gb164|BG127438 tomato 39 89 BDL52 716 89 BDL54arabidopsis|gb165|AT2G41090 arabidopsis 40 90 BDL56arabidopsis|gb165|AT2G32990 arabidopsis 41 91 BDL59arabidopsis|gb165|AT5G07110 arabidopsis 42 92 BDL59 717 92 BDL60arabidopsis|gb165|AT2G45200 arabidopsis 43 93 BDL65arabidopsis|gb165|AT4G20360 arabidopsis 44 94 BDL67arabidopsis|gb165|AT1G73600 arabidopsis 45 95 BDL68arabidopsis|gb165|AT2G17280 arabidopsis 46 96 BDL78arabidopsis|gb165|AT3G26520 arabidopsis 47 97 BDL78 718 720 BDL82arabidopsis|gb165|AT1G21790 arabidopsis 48 98 BDL89rice|gb157.2|AA749665 rice 49 99 BDL89 49 721 BDL95rice|gb157.2|AU062876 rice 50 100 BDL95 50 722 Table 1: Provided are theidentified genes, their annotation, organism and polynucleotide andpolypeptide sequence identifiers. Note that SEQ ID NOs: 716-719 and724-741 are variants of the identified polynucleotides and SEQ ID NOs:720-723 and 742-754 are variants of the identified polypeptides.

Example 2 Identification of Homologous Sequences that Increase SeedYield, Oil Yield, Growth Rate, Oil Content, Biomass, Vigor, ABST and/orNUE of a Plant

The search and identification of homologous genes involves the screeningof sequence information available, for example, in public databases suchas the DNA Database of Japan (DDBJ), Genbank, and the European MolecularBiology Laboratory Nucleic Acid Sequence Database (EMBL) or versionsthereof or the MIPS database. A number of different search algorithmshave been developed, including but not limited to the suite of programsreferred to as BLAST programs. There are five implementations of BLAST,three designed for nucleotide sequence queries (BLASTN, BLASTX, andTBLASTX) and two designed for protein sequence queries (BLASTP andTBLASTN) (Coulson, Trends in Biotechnology: 76-80, 1994; Birren et al.,Genome Analysis, I: 543, 1997). Such methods involve alignment andcomparison of sequences. The BLAST algorithm calculates percent sequenceidentity and performs a statistical analysis of the similarity betweenthe two sequences. The software for performing BLAST analysis ispublicly available through the National Centre for BiotechnologyInformation. Other such software or algorithms are GAP, BESTFIT, FASTAand TFASTA. GAP uses the algorithm of Needleman and Wunsch (J. Mol.Biol. 48: 443-453, 1970) to find the alignment of two complete sequencesthat maximizes the number of matches and minimizes the number of gaps.

The homologous genes may belong to the same gene family. The analysis ofa gene family may be carried out using sequence similarity analysis. Toperform this analysis one may use standard programs for multiplealignments e.g. Clustal W. A neighbour-joining tree of the proteinshomologous to the genes in this invention may be used to provide anoverview of structural and ancestral relationships. Sequence identitymay be calculated using an alignment program as described above. It isexpected that other plants will carry a similar functional gene(ortholog) or a family of similar genes and those genes will provide thesame preferred phenotype as the genes presented here. Advantageously,these family members may be useful in the methods of the invention.Example of other plants are included here but not limited to, barley(Hordeum vulgare), Arabidopsis (Arabidopsis thaliana), maize (Zea mays),cotton (Gossypium spp.), Oilseed rape (Brassica napus), Rice (Oryzasativa), Sugar cane (Saccharum officinarum), Sorghum (Sorghum bicolor),Soybean (Glycine max), Sunflower (Helianthus annuus), Tomato(Lycopersicon esculentum), Wheat (Triticum aestivum).

The above-mentioned analyses for sequence homology can be carried out ona full-length sequence, but may also be based on a comparison of certainregions such as conserved domains. The identification of such domains,would also be well within the realm of the person skilled in the art andwould involve, for example, a computer readable format of the nucleicacids of the present invention, the use of alignment software programsand the use of publicly available information on protein domains,conserved motifs and boxes. This information is available in the PRODOM(biochem (dot) ucl (dot) ac (dot) uk/bsm/dbbrowser/protocol/prodomqry(dot) html), PIR (pir (dot) Georgetown (dot) edu/) or Pfam (sanger (dot)ac (dot) uk/Software/Pfam/) database. Sequence analysis programsdesigned for motif searching may be used for identification offragments, regions and conserved domains as mentioned above. Preferredcomputer programs include, but are not limited to, MEME, SIGNALSCAN, andGENESCAN.

A person skilled in the art may use the homologous sequences providedherein to find similar sequences in other species and other organisms.Homologues of a protein encompass, peptides, oligopeptides,polypeptides, proteins and enzymes having amino acid substitutions,deletions and/or insertions relative to the unmodified protein inquestion and having similar biological and functional activity as theunmodified protein from which they are derived. To produce suchhomologues, amino acids of the protein may be replaced by other aminoacids having similar properties (conservative changes, such as similarhydrophobicity, hydrophilicity, antigenicity, propensity to form orbreak a-helical structures or 3-sheet structures). Conservativesubstitution tables are well known in the art (see for example Creighton(1984) Proteins. W.H. Freeman and Company). Homologues of a nucleic acidencompass nucleic acids having nucleotide substitutions, deletionsand/or insertions relative to the unmodified nucleic acid in questionand having similar biological and functional activity as the unmodifiednucleic acid from which they are derived.

Genes identified in publicly available sequence databases as sharinghigh sequence homology to the arabidopsis genes identified herein aresummarized in Table 2 below. Those genes are expected to increase plantyield, seed yield, oil yield, oil content, growth rate, biomass, vigor,ABST and/or NUE of a plant have been identified from the databases usingBLAST software (BLASTP and TBLASTN) and are provided in Table 2,hereinbelow.

TABLE 2 Homologous polynucleotides and polypeptides Polynuc. Polypept.Homology SEQ ID SEQ ID to SEQ ID % NO: Cluster name Organism NO: NO:identity Algorithm 101 brachypodium|gb169|BE415618 brachypodium 379 5188.81 blastp 102 maize|gb169.2|AI600710 maize 380 51 90.11 blastp 103maize|gb169.2|AI615263 maize 381 51 91.09 blastp 104sorghum|gb161.crp|AI987481 sorghum 382 51 81.43 blastp 105sugarcane|gb157.3|CA081111 sugarcane 383 51 86.03 blastp 106switchgrass|gb167|FE601953 switchgrass 384 51 90.99 blastp 107wheat|gb164|BE490052 wheat 385 51 89.72 blastp 108 b_rapa|gb162|EX108797b_rapa 386 52 80 tblastn 109 b_rapa|gb162|EX138742 b_rapa 387 52 92.86blastp 110 canola|gb161|CD821478 canola 388 52 80 tblastn 111radish|gb164|EV569061 radish 389 52 83.1 tblastn 112antirrhinum|gb166|AJ558675 antirrhinum 390 53 80.77 blastp 113apple|gb157.3|CN580529 apple 391 53 80.22 blastp 114arabidopsis|gb165|AT5G15750 arabidopsis 392 53 88.46 blastp 115b_rapa|gb162|CX270798 b_rapa 393 53 93.41 blastp 116b_rapa|gb162|DY009670 b_rapa 394 53 99.45 blastp 117 bean|gb167|CA897445bean 395 53 84.07 blastp 118 cacao|gb167|CU485233 cacao 396 53 84.07blastp 119 canola|gb161|EE456125 canola 397 53 91.21 blastp 120centaurea|gb166|EH737065 centaurea 398 53 80.22 blastp 121citrus|gb166|CB304606 citrus 399 53 82.97 blastp 122cotton|gb164|BQ409188 cotton 400 53 86.26 blastp 123cowpea|gb166|FF400239 cowpea 401 53 82.97 blastp 124cynara|gb167|GE592319 cynara 402 53 81.32 blastp 125dandelion|gb161|DY824742 dandelion 403 53 81.87 tblastn 126grape|gb160|CA813426 grape 404 53 84.07 blastp 127ipomoea|gb157.2|CJ749317 ipomoea 405 53 83.06 blastp 128kiwi|gb166|FG473358 kiwi 406 53 82.42 blastp 129lettuce|gb157.2|DW152666 lettuce 407 53 80.77 blastp 130melon|gb165|EB714755 melon 408 53 80.22 tblastn 131radish|gb164|EV526240 radish 409 53 95.63 blastp 132radish|gb164|EV537766 radish 410 53 95.63 blastp 133soybean|gb168|BE202985 soybean 411 53 83.52 blastp 134soybean|gb168|BM139685 soybean 412 53 83.52 blastp 135sunflower|gb162|CD851096 sunflower 413 53 82.97 blastp 136thellungiella|gb167|BY810244 thellungiella 414 53 91.21 tblastn 137triphysaria|gb164|BM356672 triphysaria 415 53 81.87 blastp 138arabidopsis|gb165|AT1G21760 arabidopsis 416 54 92.38 blastp 139b_oleracea|gb161|AM059122 b_oleracea 417 54 99.39 blastp 140b_rapa|gb162|DN965363 b_rapa 418 54 86.06 blastp 141cotton|gb164|CO083170 cotton 419 54 80.06 tblastn 142peanut|gb167|ES716655 peanut 420 54 80.06 blastp 143radish|gb164|EV546747 radish 421 54 99.39 blastp 144soybean|gb168|AI967832 soybean 422 54 80.66 blastp 145soybean|gb168|AW395758 soybean 423 54 80.97 blastp 146arabidopsis|gb165|AT3G23530 arabidopsis 424 56 96.89 blastp 147arabidopsis|gb165|AT1G02000 arabidopsis 425 57 82.31 blastp 148arabidopsis|gb165|AT2G45315 arabidopsis 426 57 86.5 tblastn 149arabidopsis|gb165|AT4G00110 arabidopsis 427 57 83.07 blastp 150b_rapa|gb162|CV544806 b_rapa 428 57 82.42 blastp 151canola|gb161|CD830303 canola 429 57 82.65 blastp 152citrus|gb166|CK740093 citrus 430 57 83.98 blastp 153soybean|gb168|AW704756 soybean 431 57 80.78 blastp 154soybean|gb168|CB540306 soybean 432 57 81.69 blastp 155tomato|gb164|BG126144 tomato 433 57 81.01 tblastn 156b_rapa|gb162|CV432750 b_rapa 434 58 95.61 blastp 157b_rapa|gb162|ES933357 b_rapa 435 58 93.86 blastp 158canola|gb161|CN730767 canola 436 58 94.74 blastp 159canola|gb161|CX193104 canola 437 58 92.98 blastp 160canola|gb161|EE472289 canola 438 58 94.74 blastp 161canola|gb161|EV217368 canola 439 58 94.74 blastp 162cassava|gb164|DV446328 cassava 440 58 82.61 blastp 163citrus|gb166|CX667844 citrus 441 58 81.58 blastp 164papaya|gb165|EX265359 papaya 442 58 81.58 blastp 165radish|gb164|EW733783 radish 443 58 95.61 blastp 166radish|gb164|FD535333 radish 444 58 94.74 blastp 167canola|gb161|CD820476 canola 445 59 83.1 blastp 168arabidopsis|gb165|AT1G31258 arabidopsis 446 61 97.73 blastp 169arabidopsis|gb165|AT3G03240 arabidopsis 447 62 81.44 blastp 170canola|gb161|CD818131 canola 448 62 81.68 blastp 171radish|gb164|EX894603 radish 449 66 89.23 blastp 172canola|gb161|CD814119 canola 450 68 86.35 blastp 173canola|gb161|CD816209 canola 451 68 85.19 blastp 174b_rapa|gb162|ES934568 b_rapa 452 69 87.04 tblastn 175radish|gb164|EV538975 radish 453 69 84.39 blastp 176apple|gb157.3|AU223507 apple 454 71 81.18 blastp 177apple|gb157.3|CN579496 apple 455 71 82.35 blastp 178b_oleracea|gb161|AM385211 b_oleracea 456 71 97.2 blastp 179b_oleracea|gb161|AM386119 b_oleracea 457 71 83.6 blastp 180b_rapa|gb162|CX273145 b_rapa 458 71 95.6 blastp 181b_rapa|gb162|EX022604 b_rapa 459 71 96 blastp 182 bean|gb167|FE686571bean 460 71 80.31 blastp 183 cacao|gb167|EH057755 cacao 461 71 83.07blastp 184 canola|gb161|CD834729 canola 462 71 95.6 blastp 185canola|gb161|CX192269 canola 463 71 97.2 blastp 186 canola|gb161|H74607canola 464 71 95.6 blastp 187 cassava|gb164|DV443366 cassava 465 7182.35 blastp 188 citrus|gb166|BQ625207 citrus 466 71 81.57 blastp 189cotton|gb164|AI054775 cotton 467 71 82.75 blastp 190cowpea|gb166|FC457888 cowpea 468 71 80.71 blastp 191cynara|gb167|GE589236 cynara 469 71 80.4 tblastn 192dandelion|gb161|DY816357 dandelion 470 71 81.64 blastp 193grape|gb160|BM436371 grape 471 71 80.39 blastp 194lettuce|gb157.2|DW046017 lettuce 472 71 81.57 blastp 195lettuce|gb157.2|DW076019 lettuce 473 71 81.96 blastp 196lettuce|gb157.2|DW110290 lettuce 474 71 81.57 blastp 197lettuce|gb157.2|DW153964 lettuce 475 71 82.35 blastp 198lotus|gb157.2|CN825209 lotus 476 71 81.89 blastp 199melon|gb165|AM715906 melon 477 71 81.89 blastp 200 papaya|gb165|EX227683papaya 478 71 83.46 blastp 201 peach|gb157.2|BU039450 peach 479 71 81.18blastp 202 peanut|gb167|CX127963 peanut 480 71 80.48 tblastn 203poplar|gb157.2|BI068247 poplar 481 71 80.78 blastp 204prunus|gb167|BU039450 prunus 482 71 81.18 blastp 205radish|gb164|EV567811 radish 483 71 92.8 tblastn 206radish|gb164|EV568452 radish 484 71 96.8 blastp 207radish|gb164|EV572670 radish 485 71 82.4 blastp 208safflower|gb162|EL387585 safflower 486 71 80.31 blastp 209soybean|gb168|AF272360 soybean 487 71 83.07 blastp 210soybean|gb168|AL370910 soybean 488 71 82.68 blastp 211soybean|gb168|BE661209 soybean 489 71 80 tblastn 212strawberry|gb164|CO817556 strawberry 490 71 81.18 blastp 213sunflower|gb162|DY915187 sunflower 491 71 82 tblastn 214sunflower|gb162|DY939330 sunflower 492 71 82 tblastn 215walnuts|gb166|CB303475 walnuts 493 71 82.28 blastp 216walnuts|gb166|CB303477 walnuts 494 71 80.8 tblastn 217b_rapa|gb162|EX017049 b_rapa 495 72 89.87 blastp 218canola|gb161|CD831601 canola 496 72 82.28 tblastn 219radish|gb164|EV547036 radish 497 72 89.87 blastp 220canola|gb161|CX189942 canola 498 76 89.59 blastp 221 canola|gb161|H74865canola 499 77 87.26 blastp 222 castorbean|gb160|EG664225 castorbean 50077 80.91 tblastn 223 cotton|gb164|BG442642 cotton 501 77 81.82 tblastn224 grape|gb160|CB916567 grape 502 77 80.24 blastp 225poplar|gb157.2|BU816880 poplar 503 77 80.61 tblastn 226radish|gb164|EV537923 radish 504 77 86.61 blastp 227soybean|gb168|AW776674 soybean 505 77 80.29 blastp 228thellungiella|gb167|DN773780 thellungiella 506 77 94.26 tblastn 229b_juncea|gb164|EVGN00166916743135 b_juncea 507 79 88.75 blastp 230b_rapa|gb162|CX268133 b_rapa 508 79 90.95 blastp 231radish|gb164|EV573167 radish 509 79 89.73 tblastn 232radish|gb164|EW724071 radish 510 83 86.58 blastp 233b_rapa|gb162|EE517848 b_rapa 511 85 80.4 blastp 234canola|gb161|CD824985 canola 512 86 80.54 blastp 235b_oleracea|gb161|AM057266 b_oleracea 513 88 90.82 blastp 236b_rapa|gb162|BG543561 b_rapa 514 88 87.87 blastp 237canola|gb161|CD820232 canola 515 88 88.2 blastp 238canola|gb161|DW999252 canola 516 88 87.87 blastp 239potato|gb157.2|BG351102 potato 517 89 89.86 blastp 240potato|gb157.2|BI435634 potato 518 89 90.65 blastp 241radish|gb164|EY917873 radish 519 92 87.1 blastp 242antirrhinum|gb166|AJ797267 antirrhinum 520 93 80.08 blastp 243apple|gb157.3|CN489681 apple 521 93 88.28 blastp 244apricot|gb157.2|CV051536 apricot 522 93 88.33 blastp 245aquilegia|gb157.3|DT735665 aquilegia 523 93 82.85 blastp 246b_oleracea|gb161|ES939061 b_oleracea 524 93 82.85 blastp 247b_rapa|gb162|EX075010 b_rapa 525 93 92.08 blastp 248basilicum|gb157.3|DY334833 basilicum 526 93 86.67 tblastn 249bean|gb167|CV530444 bean 527 93 84.94 blastp 250 cacao|gb167|CU490565cacao 528 93 89.96 blastp 251 canola|gb161|CD813794 canola 529 93 92.92blastp 252 canola|gb161|CD826913 canola 530 93 94.56 blastp 253canola|gb161|CN737313 canola 531 93 92.92 blastp 254cichorium|gb166|EH687081 cichorium 532 93 82.01 tblastn 255citrus|gb166|CF506133 citrus 533 93 89.54 blastp 256cotton|gb164|AI730510 cotton 534 93 85.77 blastp 257cotton|gb164|BF268340 cotton 535 93 82.57 blastp 258cowpea|gb166|FF384748 cowpea 536 93 85.77 blastp 259dandelion|gb161|DY819170 dandelion 537 93 81.17 tblastn 260eucalyptus|gb166|CT983920 eucalyptus 538 93 87.08 blastp 261grape|gb160|CD798978 grape 539 93 88.28 blastp 262lettuce|gb157.2|DW062039 lettuce 540 93 82.85 blastp 263melon|gb165|AM742165 melon 541 93 83.68 blastp 264poplar|gb157.2|CF234347 poplar 542 93 85.89 blastp 265poplar|gb157.2|CV241881 poplar 543 93 87.65 blastp 266potato|gb157.2|BG595485 potato 544 93 85.89 blastp 267prunus|gb167|AJ631796 prunus 545 93 88.33 blastp 268radish|gb164|EV567266 radish 546 93 92.92 blastp 269radish|gb164|EY912132 radish 547 93 92.08 blastp 270safflower|gb162|EL405854 safflower 548 93 82.01 tblastn 271soybean|gb168|BQ157726 soybean 549 93 87.45 blastp 272soybean|gb168|CD399194 soybean 550 93 86.72 blastp 273spurge|gb161|DV128393 spurge 551 93 88.7 blastp 274sunflower|gb162|DY918314 sunflower 552 93 80.83 tblastn 275thellungiella|gb167|BY820935 thellungiella 553 93 97.07 tblastn 276tobacco|gb162|EB445856 tobacco 554 93 84.71 blastp 277tomato|gb164|BG128536 tomato 555 93 86.31 blastp 278triphysaria|gb164|EY139231 triphysaria 556 93 83.4 blastp 279apple|gb157.3|CN489235 apple 557 94 83.95 blastp 280apple|gb157.3|CN490414 apple 558 94 83.78 blastp 281aquilegia|gb157.3|DR925212 aquilegia 559 94 83.64 blastp 282bean|gb167|CA905538 bean 560 94 83.06 blastp 283 bean|gb167|CB542107bean 561 94 83.27 blastp 284 canola|gb161|CD816386 canola 562 94 87.18blastp 285 canola|gb161|CX187647 canola 563 94 89.98 blastp 286citrus|gb166|BE213456 citrus 564 94 83.44 blastp 287clover|gb162|BB936594 clover 565 94 81.48 blastp 288cotton|gb164|BG440364 cotton 566 94 80.82 blastp 289cowpea|gb166|FC460131 cowpea 567 94 83.12 blastp 290cowpea|gb166|FF541811 cowpea 568 94 83.74 blastp 291grape|gb160|BG273758 grape 569 94 80.29 blastp 292 grape|gb160|BQ792941grape 570 94 81.17 blastp 293 lettuce|gb157.2|DW051431 lettuce 571 9481.82 blastp 294 lettuce|gb157.2|DW112484 lettuce 572 94 81.82 blastp295 medicago|gb157.2|AW688581 medicago 573 94 81.54 tblastn 296poplar|gb157.2|BI072710 poplar 574 94 82.51 blastp 297poplar|gb157.2|BU824581 poplar 575 94 82.79 tblastn 298potato|gb157.2|AW907286 potato 576 94 81.54 tblastn 299potato|gb157.2|BE921734 potato 577 94 82.3 blastp 300radish|gb164|EX747018 radish 578 94 80.88 blastp 301soybean|gb168|AL387670 soybean 579 94 82.4 blastp 302soybean|gb168|BG239139 soybean 580 94 82.3 blastp 303soybean|gb168|BG839432 soybean 581 94 83.37 blastp 304sunflower|gb162|CD847955 sunflower 582 94 82.1 blastp 305sunflower|gb162|CX944572 sunflower 583 94 81.57 blastp 306tobacco|gb162|GEXTOBTEFTUX1 tobacco 584 94 80.75 blastp 307tomato|gb164|BG124614 tomato 585 94 80.53 blastp 308tomato|gb164|BG125985 tomato 586 94 81.99 blastp 309canola|gb161|CD827895 canola 587 95 92.49 blastp 310canola|gb161|CX188753 canola 588 95 92.69 blastp 311 b_rapa|gb162|L35822b_rapa 589 96 88.19 blastp 312 canola|gb161|CD813792 canola 590 96 88.93blastp 313 radish|gb164|EV526073 radish 591 96 88.19 blastp 314radish|gb164|EW729491 radish 592 96 88.19 blastp 315arabidopsis|gb165|AT2G36830 arabidopsis 593 97 85.38 blastp 316b_juncea|gb164|EVGN00082509070705 b_juncea 594 97 90.51 blastp 317b_juncea|gb164|EVGN00089315240635 b_juncea 595 97 92.09 blastp 318b_juncea|gb164|EVGN00116217230337 b_juncea 596 97 90.51 blastp 319b_juncea|gb164|EVGN00256308610946 b_juncea 597 97 92.09 tblastn 320b_juncea|gb164|EVGN00465908341698 b_juncea 598 97 84.58 blastp 321b_juncea|gb164|EVGN01252008670772 b_juncea 599 97 83.79 blastp 322b_oleracea|gb161|AM385528 b_oleracea 600 97 91.3 blastp 323b_oleracea|gb161|BOU92651 b_oleracea 601 97 84.19 blastp 324b_rapa|gb162|BG545002 b_rapa 602 97 84.58 blastp 325b_rapa|gb162|BQ791222 b_rapa 603 97 86.17 blastp 326 b_rapa|gb162|L37468b_rapa 604 97 91.3 blastp 327 canola|gb161|AF118381 canola 605 97 84.19blastp 328 canola|gb161|CD815565 canola 606 97 91.3 blastp 329canola|gb161|CD824493 canola 607 97 84.58 blastp 330canola|gb161|CD841035 canola 608 97 91.3 blastp 331canola|gb161|CN729037 canola 609 97 84.58 blastp 332canola|gb161|CX187880 canola 610 97 90.91 blastp 333 radish|gb164|D84669radish 611 97 90.91 blastp 334 radish|gb164|EV549107 radish 612 97 91.3blastp 335 radish|gb164|EV569856 radish 613 97 84.58 blastp 336radish|gb164|EX748154 radish 614 97 83.79 blastp 337thellungiella|gb167|EE683447 thellungiella 615 97 91.7 blastp 338canola|gb161|H74506 canola 616 98 88.54 blastp 339 radish|gb164|EW716884radish 617 98 88.19 blastp 340 barley|gb157.3|AL450752 barley 618 9982.46 blastp 341 brachypodium|gb169|BE399053 brachypodium 619 99 85.96blastp 342 cenchrus|gb166|EB653861 cenchrus 620 99 86.84 blastp 343fescue|gb161|DT683655 fescue 621 99 80.7 blastp 344leymus|gb166|CD809085 leymus 622 99 84.21 blastp 345lovegrass|gb167|DN480336 lovegrass 623 99 86.84 blastp 346lovegrass|gb167|DN480721 lovegrass 624 99 83.33 tblastn 347lovegrass|gb167|EH184046 lovegrass 625 99 85.96 blastp 348maize|gb169.2|AI586696 maize 626 99 84.21 blastp 349maize|gb169.2|AI619158 maize 627 99 85.22 blastp 350maize|gb169.2|AI619355 maize 628 99 84.21 blastp 351maize|gb169.2|AI783324 maize 629 99 85.96 blastp 352maize|gb169.2|DQ244850 maize 630 99 84.21 blastp 353maize|gb169.2|FK957562 maize 631 99 83.33 tblastn 354maize|gb169.2|X86553 maize 632 99 85.96 blastp 355 millet|gb161|CD725537millet 633 99 85.96 blastp 356 pseudoroegneria|gb167|FF359011pseudoroegneria 634 99 84.21 blastp 357 rice|gb157.3|C26798 rice 635 9983.33 blastp 358 sorghum|gb161.crp|AI621929 sorghum 636 99 87.72 blastp359 sorghum|gb161.crp|AI666179 sorghum 637 99 85.96 blastp 360sorghum|gb161.crp|CD222293 sorghum 638 99 81.58 blastp 361sugarcane|gb157.3|BQ478960 sugarcane 639 99 86.84 blastp 362sugarcane|gb157.3|BQ537227 sugarcane 640 99 85.96 blastp 363sugarcane|gb157.3|CA074008 sugarcane 641 99 85.96 blastp 364sugarcane|gb157.3|CA112912 sugarcane 642 99 81.74 blastp 365switchgrass|gb167|DN150202 switchgrass 643 99 86.84 blastp 366switchgrass|gb167|FE615220 switchgrass 644 99 86.84 blastp 367switchgrass|gb167|FL725679 switchgrass 645 99 85.96 blastp 368switchgrass|gb167|FL732781 switchgrass 646 99 86.84 blastp 369switchgrass|gb167|FL846775 switchgrass 647 99 85.34 blastp 370switchgrass|gb167|FL899116 switchgrass 648 99 85.96 blastp 371switchgrass|gb167|FL979422 switchgrass 649 99 85.96 blastp 372wheat|gb164|BE398366 wheat 650 99 84.21 blastp 373 wheat|gb164|BE399053wheat 651 99 84.21 blastp 374 barley|gb157.3|BF623452 barley 652 10085.25 tblastn 375 maize|gb169.2|AW267619 maize 653 100 80.58 tblastn 376pseudoroegneria|gb167|FF352828 pseudoroegneria 654 100 84.89 blastp 377sorghum|gb161.crp|AW438249 sorghum 655 100 86.43 blastp 378wheat|gb164|BQ905354 wheat 656 100 85.97 tblastn 755amborella|gb166|CD482678 amborella 764 97 81.03 blastp 756castorbean|gb160|AJ605571 castorbean 765 97 80.24 blastp 757cotton|gb164|AI055329 cotton 766 97 83.79 blastp 758cotton|gb164|AI731742 cotton 767 97 83.79 blastp 759liriodendron|gb166|CK744430 liriodendron 768 97 81.42 blastp 760papaya|gb165|EX246150 papaya 769 97 81.82 blastp 761periwinkle|gb164|EG554262 periwinkle 770 97 80.24 blastp 762spurge|gb161|AW990927 spurge 771 97 80.63 blastp 763tobacco|gb162|CV018899 tobacco 772 97 80.24 blastp 773canola|gb161|DW999739 canola 774 747 82.78 blastp Table 2: Provided arepolynucleotides and polypeptides which are homologous to the identifiedpolynucleotides or polypeptides of Table 1. Note that the followingpolypeptide sequences are 100% identical: SEQ ID NO: 201 is identical toSEQ ID NO: 204; SEQ ID NO: 409 is identical to SEQ ID NO: 410; SEQ IDNO: 411 is identical to SEQ ID NO: 412; SEQ ID NO: 456 is identical toSEQ ID NO: 463; SEQ ID NO: 458 is identical to SEQ ID NO: 462; SEQ IDNO: 472 is identical to SEQ ID NO: 474; SEQ ID NO: 479 is identical toSEQ ID NO: 482; SEQ ID NO: 514 is identical to SEQ ID NO: 516; SEQ IDNO: 522 is identical to SEQ ID NO: 545; SEQ ID NO: 600 is identical toSEQ ID NO: 604, 606 and 608; SEQ ID NO: 602 is identical to SEQ ID NO:607 and 609; SEQ ID NO: 610 is identical to SEQ ID NO: 611; SEQ ID NO:620 is identical to SEQ ID NO: 643; SEQ ID NO: 622 is identical to SEQID NO: 630, 634, 650 and 65; SEQ ID NO: 629 is identical to SEQ ID NO:632; SEQ ID NO: 637 is identical # to SEQ ID NO: 640 and 641; SEQ ID NO:644 is identical to SEQ ID NO: 646; SEQ ID NO: 648 is identical to SEQID NO: 649; SEQ ID NO: 650 is identical to SEQ ID NO: 651; SEQ ID NO:624 is identical to SEQ ID NO: 625.

Example 3 Production of Arabidopsis Transcription and High ThroughputCorrelation Analysis Using 44K Arabidopsis Full Genome OligonucleotideMicro-Array

To produce a high throughput correlation analysis, the present inventorsutilized an Arabidopsis thaliana oligonucleotide micro-array, producedby Agilent Technologies [chem. (dot) agilent (dot) com/Scripts/PDS (dot)asp?1Page=50879]. The array oligonucleotide represents about 40,000 A.thaliana genes and transcripts designed based on data from the TIGR ATH1v.5 database and Arabidopsis MPSS (University of Delaware) databases. Todefine correlations between the levels of RNA expression and yieldcomponents or vigor related parameters, various plant characteristics of15 different Arabidopsis ecotypes were analyzed. Among them, nineecotypes encompassing the observed variance were selected for RNAexpression analysis. The correlation between the RNA levels and thecharacterized parameters was analyzed using Pearson correlation test[davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

RNA extraction—Five tissues at different developmental stages includingroot, leaf, flower at anthesis, seed at 5 days after flowering (DAF) andseed at 12 DAF, representing different plant characteristics, weresampled and RNA was extracted using TRIZOL® Reagent (Life Technologies)from Invitrogen [invitrogen (dot) com/content (dot) cfm?pageid=469]. Forconvenience, each micro-array expression information tissue type hasreceived a Set ID as summarized in Table 3 below.

TABLE 3 Arabidopsis transcriptom experimental sets Expression Set Set IDRoot A Leaf B Flower C Seed 5 DAF D Seed 12 DAF E Table 3. Provided arethe Arabidopsis transcriptom experimental sets (A-E). DAF = days afterflowering.

Approximately 30-50 mg of tissue was taken from samples. The weighedtissues were ground using pestle and mortar in liquid nitrogen andresuspended in 500 ill of TRIzol Reagent. To the homogenized lysate, 100μl of chloroform was added followed by precipitation using isopropanoland two washes with 75% ethanol. The RNA was eluted in 30 μl ofRNase-free water. RNA samples were cleaned up using Qiagen's RNeasyminikit clean-up protocol as per the manufacturer's protocol.

Yield component and vigor related parameters assessment—eight out of thenine Arabidopsis ecotypes were used in each of 5 repetitive blocks(named A, B, C, D and E), each containing 20 plants per plot were grownat control conditions greenhouse 22° C., 20:20:20 (weight ratios) N:P:K[nitrogen (N), phosphorus (P) and potassium (K)] fertilizer was added.During this time data was collected documented and analyzed. Additionaldata was collected through the seedling stage of plants grown at tissueculture in vertical grown transparent agar plates. Most of chosenparameters were analyzed by digital imaging.

Digital imaging in Tissue culture—A laboratory image acquisition system,which consists of a digital reflex camera (Canon EOS 300D) attached witha 55 mm focal length lens (Canon EF-S series), mounted on a reproductiondevice (Kaiser RS), which included 4 light units (4×150 Watts lightbulb) and located in a darkroom, was used for capturing images ofplantlets sawn in square agar plates.

Digital imaging in Greenhouse—The image capturing process was repeatedevery 3-4 days starting at day 7 till day 30. The same camera attachedwith a 24 mm focal length lens (Canon EF series), placed in a custommade iron mount, was used for capturing images of larger plants sawn inwhite tubs in an environmental controlled greenhouse. The white tubswere square shape with measurements of 36×26.2 cm and 7.5 cm deep.During the capture process, the tubs were placed beneath the iron mount,while avoiding direct sun light and casting of shadows. This process wasrepeated every 3-4 days for up to 30 days.

An image analysis system was used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.37, Java based image processing program, which was developed at theU.S National Institutes of Health and is freely available on theinternet at rsbweb (dot) nih (dot) gov/. Images were captured inresolution of 6 Mega Pixels (3072×2048 pixels) and stored in a lowcompression JPEG (Joint Photographic Experts Group standard) format.Next, analyzed data was saved to text files and processed using the JMPstatistical analysis software (SAS institute).

Leaf analysis—Using the digital analysis leaves data was calculated,including leaf number, area, perimeter, length and width. On day 30, 3-4representative plants were chosen from each plot of blocks A, B and C.The plants were dissected, each leaf was separated and was introducedbetween two glass trays, a photo of each plant was taken and the variousparameters (such as leaf total area, laminar length etc.) werecalculated from the images (FIGS. 1 a-d). The blade circularity wascalculated as laminar width divided by laminar length.

Root analysis—During 17 days, the different ecotypes were grown intransparent agar plates. The plates were photographed every 2 daysstarting at day 7 in the photography room and the roots development wasdocumented (FIGS. 2 a-b). The growth rate was calculated according tothe formula I as described above [Relative growth arearate=(ΔArea/Δt)*(1/Area t0)].

Vegetative growth rate analysis—The growth rate was calculated bydividing the area added (Δ Area) by the number of days for each interval(Δt). The analysis was ended with the appearance of overlapping plants.The growth rate was calculated according to Formula IV.Growth rate=ΔArea/Δt.  Formula IV:

For comparison between ecotypes the calculated rate was normalized usingplant developmental stage as represented by the number of true leaves.In cases where plants with 8 leaves had been sampled twice (for exampleat day 10 and day 13), only the largest sample was chosen and added tothe Anova comparison.

Seeds in siliques analysis—On day 70, 15-17 siliques were collected fromeach plot in blocks D and E. The chosen siliques were light brown colorbut still intact. The siliques were opened in the photography room andthe seeds were scatter on a glass tray, a high resolution digitalpicture was taken for each plot. Using the images the number of seedsper silique was determined.

Seeds average weight—At the end of the experiment all seeds from plotsof blocks A-C were collected. An average weight of 0.02 grams wasmeasured from each sample, the seeds were scattered on a glass tray anda picture was taken. Using the digital analysis, the number of seeds ineach sample was calculated.

Oil percentage in seeds—At the end of the experiment all seeds fromplots of blocks A-C were collected. Columbia seeds from 3 plots weremixed grounded and then mounted onto the extraction chamber. 210 ml ofn-Hexane (Cat No. 080951 Biolab Ltd.) were used as the solvent. Theextraction was performed for 30 hours at medium heat 50° C. Once theextraction has ended the n-Hexane was evaporated using the evaporator at35° C. and vacuum conditions. The process was repeated twice. Theinformation gained from the Soxhlet extractor (Soxhlet, F. Diegewichtsanalytische Bestimmung des Milchfettes, Polytechnisches J.(Dingier's) 1879, 232, 461) was used to create a calibration curve forthe Low Resonance NMR. The content of oil of all seed samples wasdetermined using the Low Resonance NMR (MARAN Ultra—Oxford Instrument)and its MultiQuant software package.

Silique length analysis—On day 50 from sowing, 30 siliques fromdifferent plants in each plot were sampled in block A. The chosensiliques were green-yellow in color and were collected from the bottomparts of a grown plant's stem. A digital photograph was taken todetermine silique's length.

Dry weight and seed yield—On day 80 from sowing, the plants from blocksA-C were harvested and left to dry at 30° C. in a drying chamber. Thebiomass and seed weight of each plot was separated, measured and dividedby the number of plants. Dry weight=total weight of the vegetativeportion above ground (excluding roots) after drying at 30° C. in adrying chamber; Seed yield per plant=total seed weight per plant (gr).

Oil yield—The oil yield was calculated using Formula V.Seed Oil yield=Seed yield per plant (gr)*Oil % in seed  Formula V

Harvest Index—The harvest index was calculated using Formula III asdescribed above [Harvest Index=Average seed yield per plant/Average dryweight].

Experimental Results

Nine different Arabidopsis ecotypes were grown and characterized for 18parameters (named as vectors). Data parameters are summarized in Table4, below.

TABLE 4 Arabidopsis correlated parameters (vectors) Correlated parameterwith Correlation ID Root length day 13 (cm) 1 Root length day 7 (cm) 2Relative root growth (cm/day) day 13 3 Fresh weight per plant (gr) atbolting stage 4 Dry matter per plant (gr) 5 Vegetative growth rate(cm²/day) till 8 true leaves 6 Blade circularity 7 Lamina width (cm) 8Lamina length (cm) 9 Total leaf area per plant (cm) 10 1000 Seed weight(gr) 11 Oil % per seed 12 Seeds per silique 13 Silique length (cm) 14Seed yield per plant (gr) 15 Oil yield per plant (mg) 16 Harvest Index17 Leaf width/length 18 Table 4. Provided are the Arabidopsis correlatedparameters (correlation ID Nos. 1-18).

The characterized values are summarized in Tables 5 and 6 below.

TABLE 5 Arabidopsis ecotypes measured parameters Total Seed Oil Dry leafyield yield 1000 matter area per per Oil % Seed per per Seeds Siliqueplant plant per weight plant Harvest plant per length Ecotype (gr) (mg)seed (gr) (gr) Index (cm) silique (cm) An-1 0.34 118.63 34.42 0.02030.64 0.53 46.86 45.44 1.06 Col-0 0.44 138.73 31.19 0.0230 1.27 0.35109.89 53.47 1.26 Ct-1 0.59 224.06 38.05 0.0252 1.05 0.56 58.36 58.471.31 Cvi 0.42 116.26 27.76 0.0344 1.28 0.33 56.80 35.27 1.47 (N8580)Gr-6 0.61 218.27 35.49 0.0202 1.69 0.37 114.66 48.56 1.24 Kondara 0.43142.11 32.91 0.0263 1.34 0.32 110.82 37.00 1.09 Ler-1 0.36 114.15 31.560.0205 0.81 0.45 88.49 39.38 1.18 Mt-0 0.62 190.06 30.79 0.0226 1.210.51 121.79 40.53 1.18 Shakdara 0.55 187.62 34.02 0.0235 1.35 0.41 93.0425.53 1.00 Table 5. Provided are the parameters measured in Arabidopsisecotypes: Seed yield per plant (cm); oil yield per plant (mg); oil % perseed; 1000 seed weight (gr); dry matter per plant (gr); harvest index;Total leaf area per plant (cm); seeds per silique; Silique length (cm).

TABLE 6 Arabidopsis ecotypes, additional measured parameters FreshRelat. Root Root weight Leaf root length length per Lam. Lam. width/Blade Ecotype Veg. GR growth day 7 day 13 plant Leng. width lengthcircularity An-1 0.313 0.631 0.937 4.419 1.510 2.767 1.385 0.353 0.509Col-0 0.378 0.664 1.759 8.530 3.607 3.544 1.697 0.288 0.481 Ct-1 0.4841.176 0.701 5.621 1.935 3.274 1.460 0.316 0.450 Cvi 0.474 1.089 0.7284.834 2.082 3.785 1.374 0.258 0.370 (N8580) Gr-6 0.425 0.907 0.991 5.9573.556 3.690 1.828 0.356 0.501 Kondara 0.645 0.774 1.163 6.372 4.3384.597 1.650 0.273 0.376 Ler-1 0.430 0.606 1.284 5.649 3.467 3.877 1.5100.305 0.394 Mt-0 0.384 0.701 1.414 7.060 3.479 3.717 1.817 0.335 0.491Shakdara 0.471 0.782 1.251 7.041 3.710 4.149 1.668 0.307 0.409 Table 6.Provided are the parameters measured in Arabidopsis ecotypes: Veg. GR =vegetative growth rate (cm²/day) until 8 true leaves; Relat. Root growth= relative root growth (cm/day); Root length day 7 (cm); Root length day13 (cm); fresh weight per plant (gr) at bolting stage; Lam. Leng. =Lamima length (cm); Lam. Width = Lamina width (cm); Leaf width/length;Blade circularity.

Tables 7-9, below, provide the selected genes, the characterizedparameters (which are used as x axis for correlation) and the correlatedtissue transcription along with the correlation value (R, calculatedusing Pearson correlation).

TABLE 7 Arabidopsis selected genes and their correlation with yield(seed yield, oil yield, oil content), biomass, growth rate and/or vigorcomponents among different transcriptom sets Gene Cluster Exp. Corr.Exp. Corr. Exp. Corr. Exp. Corr. Name Name Set Vec. R Set Vec. R SetVec. R Set Vec. R BDL112 AT3G23510 B 7 0.84 D 17 0.90 D 8 −0.88 D 10−0.94 BDL113 AT2G45310 B 8 0.85 D 13 0.91 D 14 0.91 B 10 0.83 BDL114AT5G27820 B 16 0.92 D 6 0.88 B 15 0.94 BDL115 AT4G11090 C 8 0.85 A 80.81 C 10 0.90 B 10 0.92 BDL116 AT4G24175 B 16 0.92 B 15 0.91 BDL119AT3G47965 D 16 0.93 D 15 0.93 BDL120 AT3G03230 D 16 0.95 D 3 0.89 D 150.96 BDL122 AT3G49000 D 17 0.96 D 8 −0.92 D 10 −0.96 E 6 0.83 BDL123AT2G21860 A 16 0.82 D 13 0.92 D 14 0.94 A 15 0.86 BDL124 AT5G51590 D 120.93 C 16 0.93 A 1 −0.85 D 2 −0.91 BDL125 AT3G16180 D 16 −0.91 D 15−0.93 BDL128 AT1G60770 D 5 −0.86 D 17 0.82 D 8 −0.91 E 14 0.88 BDL130AT3G03870 C 9 −0.82 D 13 0.85 D 14 0.94 C 6 −0.85 BDL131 AT4G27450 A 10.92 A 2 0.91 BDL132 AT4G23730 D 13 0.90 C 14 0.94 D 14 0.91 C 3 0.81BDL133 AT3G06150 D 13 0.86 C 14 0.90 D 14 0.92 BDL134 AT3G28420 D 130.92 D 14 0.91 E 3 0.81 E 11 0.80 BDL135 AT3G18600 D 12 0.96 D 16 0.87 D3 0.93 D 2 −0.81 BDL136 AT3G22990 D 12 0.90 C 16 0.83 D 16 0.81 D 3 0.89BDL137 AT5G14530 B 12 0.83 D 12 0.83 B 16 0.83 D 16 0.81 BDL139AT1G29800 C 13 0.87 B 13 0.85 BDL141 AT1G29980 D 18 0.90 C 16 0.81 B 160.81 A 3 0.83 BDL142 AT2G39110 C 16 0.82 D 16 0.83 B 3 0.86 D 3 0.90BDL143 AT1G62810 A 13 0.94 D 13 0.82 BDL144 AT3G14890 C 16 0.81 BDL145AT1G24470 C 13 0.89 B 13 0.82 D 14 0.80 BDL146 AT3G09310 C 14 0.85 C 30.81 B 3 0.92 A 3 0.91 BDL148 AT4G35785 D 17 0.91 D 10 −0.93 D 4 −0.89 B3 0.82 BDL42 AT5G13170 B 11 0.88 BDL51 AT5G64260 E 9 −0.81 A 8 −0.82 D13 −0.83 B 14 0.82 BDL54 AT2G41090 A 6 0.82 B 3 −0.86 BDL60 AT2G45200 C7 −0.88 BDL65 AT4G20360 B 7 −0.81 B 18 −0.88 B 12 −0.82 D 11 0.89 BDL78AT3G26520 D 13 0.84 BDL149 AT5G15750 B 14 0.83 BDL149 AT5G15750 A 140.95 B 3 0.89 Table 7. Provided are Arabidopsis selected genes and theircorrelation with yield (seed yield, oil yield, oil content), biomass,growth rate and/or vigor components among different transcriptom sets.Corr. Vec. = correlation vector; Exp. Set = experimental set.

TABLE 8 Arabidopsis selected genes and their correlation with yield(seed yield, oil yield, oil content), biomass, growth rate and/or vigorcomponents among different transcriptom sets Gene Cluster Exp. Corr.Exp. Corr. Exp. Corr. Exp. Corr. Name Name Set Vec. R Set Vec. R SetVec. R Set Vec. R BDL112 AT3G23510 D 4 −0.84 BDL113 AT2G45310 BDL114AT5G27820 BDL115 AT4G11090 A 10 0.82 D 10 0.80 C 4 0.93 B 4 0.96 BDL116AT4G24175 BDL119 AT3G47965 BDL120 AT3G03230 BDL122 AT3G49000 D 4 −0.84 A11 0.88 BDL123 AT2G21860 BDL124 AT5G51590 C 15 0.91 BDL125 AT3G16180BDL128 AT1G60770 D 10 −0.84 A 1 −0.94 D 1 −0.86 A 2 −0.83 BDL130AT3G03870 BDL131 AT4G27450 BDL132 AT4G23730 BDL133 AT3G06150 BDL134AT3G28420 BDL135 AT3G18600 BDL136 AT3G22990 BDL137 AT5G14530 C 3 0.89 D3 0.89 BDL139 AT1G29800 BDL141 AT1G29980 C 15 0.84 B 15 0.85 BDL142AT2G39110 BDL143 AT1G62810 BDL144 AT3G14890 BDL145 AT1G24470 BDL146AT3G09310 A 2 −0.81 C 11 0.83 D 11 0.81 BDL148 AT4G35785 E 1 0.88 BDL42AT5G13170 BDL51 AT5G64260 D 14 −0.88 E 6 −0.82 B 11 0.87 BDL54 AT2G41090BDL60 AT2G45200 BDL65 AT4G20360 BDL78 AT3G26520 BDL149 AT5G15750 BDL149AT5G15750 D 4 −0.84 Table 8. Provided are Arabidopsis selected genes andtheir correlation with yield (seed yield, oil yield, oil content),biomass, growth rate and/or vigor components among differenttranscriptom sets. Corr. Vec. = correlation vector; Exp. Set =experimental set.

TABLE 9 Arabidopsis selected genes and their correlation with yield(seed yield, oil yield, oil content), biomass, growth rate and/or vigorcomponents among different transcriptom sets Gene Name Cluster Name Exp.Set Corr. Vec. R BDL112 AT3G23510 BDL113 AT2G45310 BDL114 AT5G27820BDL115 AT4G11090 A 4 0.86 BDL116 AT4G24175 BDL119 AT3G47965 BDL120AT3G03230 BDL122 AT3G49000 BDL123 AT2G21860 BDL124 AT5G51590 BDL125AT3G16180 BDL128 AT1G60770 BDL130 AT3G03870 BDL131 AT4G27450 BDL132AT4G23730 BDL133 AT3G06150 BDL134 AT3G28420 BDL135 AT3G18600 BDL136AT3G22990 BDL137 AT5G14530 BDL139 AT1G29800 BDL141 AT1G29980 BDL142AT2G39110 BDL143 AT1G62810 BDL144 AT3G14890 BDL145 AT1G24470 BDL146AT3G09310 BDL148 AT4G35785 BDL42 AT5G13170 BDL51 AT5G64260 BDL54AT2G41090 BDL60 AT2G45200 BDL65 AT4G20360 BDL78 AT3G26520 BDL149AT5G15750 BDL149 AT5G15750 Table 9. Provided are Arabidopsis selectedgenes and their correlation with yield (seed yield, oil yield, oilcontent), biomass, growth rate and/or vigor components among differenttranscriptom sets. Corr. Vec. = correlation vector; Exp. Set =experimental set.

Tables 10 and 11, below, provide data about the homologous of selectedgenes, the characterized parameters (which are used as x axis forcorrelation) and the correlated tissue transcription along with thecorrelation value (R, calculated using Pearson correlation).

TABLE 10 Homologous of Arabidopsis selected genes and their correlationwith yield (seed yield, oil yield, oil content), biomass, growth rateand/or vigor components among different transcriptom sets Gene ClusterExp. Corr. Exp. Corr. Exp. Corr. Exp. Corr. Name Name Set Vec. R SetVec. R Set Vec. R Set Vec. R BDL110_H0 AT1G21760 D 17 0.97 D 8 −0.81 B16 0.82 D 10 −0.91 BDL113_H0 AT1G02000 B 13 −0.81 BDL113_H1 AT2G45315 E1 0.92 BDL113_H2 AT4G00110 B 18 −0.84 E 14 0.87 E 11 0.87 BDL120_H0AT3G03240 D 16 0.89 C 4 −0.84 D 15 0.88 BDL78_H0 AT2G36830 B 12 −0.92 C2 −0.87 Table 10. Provided are homologous of Arabidopsis selected genesand their correlation with yield (seed yield, oil yield, oil content),biomass, growth rate and/or vigor components among differenttranscriptom sets. Corr. Vec. = correlation vector; Exp. Set =experimental set.

TABLE 11 Homologous of Arabidopsis selected genes and their correlationwith yield (seed yield, oil yield, oil content), biomass, growth rateand/or vigor components among different transcriptom sets Gene ClusterExp. Corr. Exp. Corr. Exp. Corr. Exp. Corr. Name Name Set Vec. R SetVec. R Set Vec. R Set Vec. R BDL110_H0 AT1G21760 D 4 −0.85 B 3 0.81 A 30.92 B 15 0.87 BDL113_H0 AT1G02000 BDL113_H1 AT2G45315 BDL113_H2AT4G00110 BDL120_H0 AT3G03240 BDL78_H0 AT2G36830 Table 11. Provided arehomologous of Arabidopsis selected genes and their correlation withyield (seed yield, oil yield, oil content), biomass, growth rate and/orvigor components among different transcriptom sets. Corr. Vec. =correlation vector; Exp. Set = experimental set.

Example 4 Gene Cloning and Generation of Binary Vectors for PlantExpression

To validate their role in improving oil content, plant yield, seedyield, biomass, growth rate, ABST, NUE and/or vigor, selected genes wereover-expressed in plants, as follows.

Cloning Strategy

Genes listed in Example 1 hereinabove were cloned into binary vectorsfor the generation of transgenic plants. For cloning, the full-lengthopen reading frame (ORF) was first identified. In case of ORF-ESTclusters and in some cases already published mRNA sequences wereanalyzed to identify the entire open reading frame by comparing theresults of several translation algorithms to known proteins from otherplant species. To clone the full-length cDNAs, reverse transcription(RT) followed by polymerase chain reaction (PCR; RT-PCR) was performedon total RNA extracted from leaves, flowers, siliques or other planttissues, growing under normal conditions. Total RNA was extracted asdescribed in Example 2 above. Production of cDNA and PCR amplificationwas performed using standard protocols described elsewhere (Sambrook J.,E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A LaboratoryManual., 2nd Ed. Cold Spring Harbor Laboratory Press, New York.) whichare well known to those skilled in the art. PCR products were purifiedusing PCR purification kit (Qiagen). In case where the entire codingsequence was not found, RACE kit from Ambion (RACE=_R_apid_A_ccess to_c_DNA_E_nds) was used to access the full cDNA transcript of the genefrom the RNA samples described above. The RACE procedure was performedfor the genes BDL-108 (SEQ ID NO:726), BDL-110 (SEQ ID NO:728) andBDL-111 (SEQ ID NO:730) using the primers sequences listed in Table 12,below. RACE products were cloned into high copy vector followed bysequencing. The information from the RACE procedure was used for cloningof the full length ORF of the corresponding genes.

TABLE 12RACE primers used for sequencing of the identified genes of the inventionHigh copy plasmid  used for cloning Gene NamePrimers used for amplification of RACE products BDL108_RaceFwd: BDL108_Outer_Race (SEQ ID NO: 853): Topo TAGCTATACAACATGGGAGTTATACC BDL108_RaceFwd: BDL108_Inner_Race (SEQ ID NO: 854): CTATCGACAGTGCTGGTACABDL108_Race Rev: 3′ Race Outer Primer (SEQ ID NO: 855):GCGAGCACAGAATTAATACGACT BDL108_Race Rev: 3′Race Inner Primer (SEQ ID NO: 856): CGCGGATCCGAATTAATACGACTCACTATAGGBDL110_Race Fwd: BDL110_Outer_Race (SEQ ID NO: 857): Topo TATGCAGTCTAAATACGATGGATCA BDL110_RaceFwd: BDL110_Inner_Race (SEQ ID NO: 858): GAGTAGGAACACTTACATTCGABDL110_Race Rev: 3′ Race Outer Primer (SEQ ID NO: 859):GCGAGCACAGAATTAATACGACT BDL110_Race Rev: 3′Race Inner Primer(SEQ ID NO: 860): CGCGGATCCGAATTAATACGACTCACTATAGGBDL111_Race Fwd: BDL111_Outer_Race (SEQ ID NO: 861): Topo TATCTCAAGAAGCTCTTCGTGGA BDL111_RaceFwd: BDL111_Inner_Race (SEQ ID NO: 862): GAGGAAGAATCTGAGCCGATBDL111_Race Rev: 3′ Race Outer Primer (SEQ ID NO: 863):GCGAGCACAGAATTAATACGACT BDL111_Race Rev: 3′Race Inner Primer (SEQ ID NO: 864): CGCGGATCCGAATTAATACGACTCACTATAGGTable 12. Provided are the PCR primers used for RACE sequencing. Fwd =forward primer; Rev = reverse primer;

In case genomic DNA was cloned, as in the case of BDL119 gene, the genewas amplified by direct PCR on genomic DNA extracted from leaf tissueusing the DNEASY® (QIAGEN GmbH) kit (Qiagen Cat. No. 69104).

Usually, 2 sets of primers were synthesized for the amplification ofeach gene from a cDNA or a genomic sequence; an external set of primersand an internal set (nested PCR primers). When needed (e.g., when thefirst PCR reaction did not result in a satisfactory product forsequencing), an additional primer (or two) of the nested PCR primerswere used. Table 13 below provides primers used for cloning of selectedgenes.

TABLE 13 The PCR primers used for cloning the genes of the inventionRestriction Enzymes used Gene Name for cloningPrimers used for amplification BDL42 SalI, XbaIFwd Nested: BDL42_NF_SalI (SEQ ID NO: 865)AATGTCGACAGAAAATGGGAGTCATGATCAAFwd External: BDL42_EF_SalI (SEQ ID NO: 866)AATGTCGACTGCTATAGAAAATGGGAGTCATGRev Nested: BDL42_NR_XbaI (SEQ ID NO: 867)TATCTAGATCATCAAACGGTTTCAGGACGAGRev External: BDL42_ER_XbaI (SEQ ID NO: 868)TATCTAGATGACACTTCAAACGGTTTCAG BDL46 SalI, SmaI Fwd Nested: BDL46_NF_S alI (SEQ ID NO: 869) ACGGTCGACACTTGATGACAATGGGCGACRev Nested: BDL46_NR_SmaI (SEQ ID NO: 870)TCCCGGGTTATTACCTACAAGTAGATGATTCTACACC BDL51 XbaI, SacIFwd: BDL51_F_XbaI (SEQ ID NO: 871) AATCTAGATCTCAATGGCTTCTAATTACCGRev Nested: BDL51_NR_SacI (SEQ ID NO: 872) AGAGCTCGTGTCTTACTCACATCCCTTGGRev External: BDL51_ER_SacI (SEQ ID NO: 873)TGAGCTCTGCCACGTGTCTTACTCACATC BDL52 SalI, XbaIFwd: BDL52_F_SalI (SEQ ID NO: 874) AATGTCGACCTATAATGGCTGGAATGTGTTGRev Nested: BDL52_NR_XbaI (SEQ ID NO: 875)TATCTAGATTACCCATACTGTTATAGATTTTTTCTCRev External: BDL52_ER_XbaI (SEQ ID NO: 876)TATCTAGACATCAACAAAGGCAGCTAAATC BDL54 SalI, XbaIFwd Nested: BDL54_NF_SalI (SEQ ID NO: 877)AATGTCGACAAACAATGGCGAATAAGTTCACRev Nested: BDL54_NR_XbaI (SEQ ID NO: 878)TATCTAGATCATCAAGAAAACAACGCTTCG BDL56 SalI, XbaIFwd Nested: BDL56_NF_SalI (SEQ ID NO: 879)AGCGTCGACCAAATATGACTGTGATGAATCACCFwd External: BDL56_EF_SalI (SEQ ID NO: 880)ATAGTCGACAAAGAGATCTTCACAAATATGACTGRev Nested: BDL56_NR_XbaI (SEQ ID NO: 881)TATCTAGACTACTATCTCTTATAAGTTGCAACCAAGRev External: BDL56_ER_XbaI (SEQ ID NO: 882)TATCTAGAATAGAAATGGCAAAATGGGTG BDL59 SalI, XbaIFwd Nested: BDL59_NF_SalI (SEQ ID NO: 883) AATGTCGACCTGCAATGGCTTCTCCTCTTFwd External: BDL59_EF_SalI (SEQ ID NO: 884)TAAGTCGACGATCTCTCTCTGCACTCTCTGACRev Nested: BDL59_NR_XbaI (SEQ ID NO: 885)TATCTAGATCAATCTCAGACTCGAACGCGTGRev External: BDL59_ER_XbaI (SEQ ID NO: 886)TATCTAGACTTCAACAATCTCAGACTCGAAC BDL60 SalI, SacIFwd Nested: BDL60_NF_SalI (SEQ ID NO: 887) AGAGCTCAGGAAAATGACAGAATCGAGTCFwd External: BDL60_EF_SalI (SEQ ID NO: 888)AATGTCGACGGAGAGGTTACTGATCTGAATTGRev Nested: BDL60_NR_SacI (SEQ ID NO: 889) AGAGCTCAGGAAAATGACAGAATCGAGTCRev External: BDL60_ER_SacI (SEQ ID NO: 890)TGAGCTCAGCTTAGGTGTATGAACATTCTG BDL65 SalI, SmaIFwd Nested: BDL65_NF_SalI (SEQ ID NO: 891) AATGTCGACTCCAATTCCATCTTCCCATGFwd External: BDL65_EF_SalI (SEQ ID NO: 892)AATGTCGACCTCTCCTCTGCTCTCCAATTCRev Nested: BDL65_NR_SmaI (SEQ ID NO: 893) TCCCGGGTCATCATTCGAGGATCGTCCCARev External: BDL65_ER_SmaI (SEQ ID NO: 894)TCCCGGGCTTATAATCATTCGAGGATCGT BDL67 SalI, SmaIFwd Nested: BDL67_NF_SalI (SEQ ID NO: 895) AATGTCGACGGATAATGGCTTCGTATGGCRev Nested: BDL67_NR_SmaI (SEQ ID NO: 896)TCCCGGGTTATCAGTTTCTCTTGGCGATGA BDL68 SalI, XbaIFwd Nested: BDL68_NF_SalI (SEQ ID NO: 897) TAGGTCGACTAGCCATGGACAACGAAGGRev Nested: BDL68_NR_XbaI (SEQ ID NO: 898)TATCTAGATTATTAGCCACTAGGATTATCAAGTC BDL78 XbaI, SacIFwd: BDL78_F_XbaI (SEQ ID NO: 899) AATCTAGATCCGATCATGCCGACCAGRev Nested: BDL78_NR_SacI-new (SEQ ID NO: 900)TGAGCTCTTATCAGTAATCGGTGGTAGGCARev External: BDL78_ER_SacI (SEQ ID NO: 901)TGAGCTCCAGATTAACAACGTTGAATTTGAC BDL82 SalI, XbaIFwd Nested: BDL82_NF_SalI (SEQ ID NO: 902)AAGGTCGACCGAGAGAGACAGAGAGGTTTCGFwd External: BDL82_EF_SalI (SEQ ID NO: 903)ATAGTCGACCGAAGTTTGAGCTAAGAATCCRev Nested: BDL82_NR_XbaI (SEQ ID NO: 904)TATCTAGATTATTATTCTCCATGGTCGTGAAGRev External: BDL82_ER_XbaI (SEQ ID NO: 905)TATCTAGATAGCTATTATTCTCCATGGTCG BDL89 SalI, XbaIFwd Nested: BDL89_NF_SalI (SEQ ID NO: 906)AATGTCGACCCAGGATGAAGTTCATTTCTGFwd External: BDL89_EF_SalI (SEQ ID NO: 907)AATGTCGACTCTCTCCATCTCCCATCCAG Rev Nested: BDL89_NR_XbaI (SEQ ID NO: 908)TATCTAGATCATCGCATCACTCAGTCAAACAAACRev External: BDL89_ER_XbaI (SEQ ID NO: 909)TATCTAGAGATGATAGAAGAGGTGACCGC BDL95Short SalI, SacIFwd: BDL95_Short_F (SEQ ID NO: 910) AATGTCGACGGCGAATGGCTGGATTTCRev Nested: BDL95_NR_SacI (SEQ ID NO: 911)TGAGCTCTTATCAGTCCTGATGTGTCTGCTG BDL100 SalI, SacIFwd Nested: BDL100_NF_SalI (SEQ ID NO: 912)AATGTCGACAACAATGGAGAGCGAGATGGCGFwd External: BDL100_EF_SalI (SEQ ID NO: 913)AATGTCGACGAGGAGGAACAAACAACTCATCRev Nested: BDL100_NR_SacI (SEQ ID NO: 914)TGAGCTCTCATCATTGAATCATCGGATCACCRev External: BDL100_ER_sacI (SEQ ID NO: 915)TGAGCTCGCAGGTCATTGAATCATCGG BDL106 XbaI, SacIFwd Nested: BDL106_NF_XbaI (SEQ ID NO: 916) ATTCTAGAAAACCATGACCGTCGTCTCFwd External: BDL106_EF_XbaI (SEQ ID NO: 917)CTTCTAGAGGTCTCTTCTCAGATACTCATTCACRev Nested: BDL106_NR_SacI (SEQ ID NO: 918)TGAGCTCTTATTAGAATCTGCAGAAAGCTAGRev External: BDL106_ER_SacI (SEQ ID NO: 919)TGAGCTCAGATGTCAAAGAGGGCTTACTC BDL108 SalI, XbaIFwd Nested: BDL108_NF_SalI (SEQ ID NO: 920)AATGTCGACCAGTGATGAGGAAGCTCAAGAFwd External: BDL108_EF_SalI (SEQ ID NO: 921)ATAGTCGACCGTTGTTTGCACCACCTTG Rev Nested: BDL108_NR_XbaI (SEQ ID NO: 922)TATCTAGATTATTAAGCAAGCATGTCGTAGTCARev External: BDL108_ER_XbaI (SEQ ID NO: 923)TCTCTAGATTAGATCTTTTAAGCAAGCATGTCG BDL110 SalI, XbaIFwd Nested: BDL110_NF_SalI (SEQ ID NO: 924)ACGGTCGACTCCACATGACTTCAGATGCTCFwd External: BDL110_EF_SalI (SEQ ID NO: 925)ACTGTCGACGAACATCACCCAATTCTCTAGCRev Nested: BDL110_NR_xbaI (SEQ ID NO: 926)TATCTAGACTACTAGCCGGTGACAAAGTAATCRev External: BDL110_ER_XbaI (SEQ ID NO: 927)TATCTAGACTAATCGTTGGTTGATGTGTCACTCTAG BDL111 EcoRVFwd Nested: BDL111_NF_EcoRV(SEQ ID NO: 928)TAGATATCAAAAGATGCAAGTTGTTTCTCCFwd External: BDL111_EF_EcoRV (SEQ ID NO: 929)TAGATATCCTGTGTGTTTGTATTTATTTGGATCRev Nested: BDL111_NR_EcoRV(SEQ ID NO: 930)TAGATATCTCATGATGATCAGTAAGGATGAACATTCRev External: BDL111_ER_EcoRV (SEQ ID NO: 931)TAGATATCTCAGCAAGAAGGTGATGATCAGTAAGG BDL112 SalI, XbaIFwd Nested: BDL112_NF_SI (SEQ ID NO: 932)TTGGTCGACCGTAGACACGTATTTTGAAGGGFwd External: BDL112_EF_SI (SEQ ID NO: 933)TATGTCGACTTAATGGTAGACCGTAGACACGRev Nested: BDL112_NR_Xb (SEQ ID NO: 934)CAATCTAGATTAATGCTCTCAAGAGACACAATAAGCRev External: BDL112_ER_Xb (SEQ ID NO: 935)CTTCTAGATTAGGTCATCAAATATTGTATAGATCG BDL113_GA SacI, XbaISynthetic product BDL114 SalI, XbaI Fwd: BDL114_NF_SI(SEQ ID NO: 936)TTTGTCGACTCAGCTTCAGATGGTGATTCC Rev: BDL114_NR_Xb (SEQ ID NO: 937)TTTCTAGATCATCAGAGCAACTTGACACCAGC BDL115 SmaI, SacIFwd Nested: BDL115_NF_SmaI (SEQ ID NO: 938)ACCCGGGAGAAGATGAAGCTAAAATGGGAAFwd External: BDL115_EF_SmaI (SEQ ID NO: 939)ACCCGGGGTATATCTCTCAGCGCGAGG Rev Nested: BDL115_NR_SacI (SEQ ID NO: 940)TGAGCTCTTATTATTTACCGGTTCGACCATTRev External: BDL115_ER_SacI (SEQ ID NO: 941)TGAGCTCTTAGCCATTGACTACATACAAGCAA BDL116 EcoRVFwd Nested: BDL116_NF_EcRV (SEQID NO: 942 TAGATATCACCTTGGAACGATTTTGCCFwd External: BDL116_EF_EcRV (SEQ ID NO: 943)GAGATATCAAAGCTCTGACCTTGGAACG Rev Nested: BDL116_NR_EcRV (SEQ ID NO: 944)CAGATATCTTATCATAAGTACAAATCAGTCTGCTCACRev External: BDL116_ER_EcRV(SEQ ID NO: 945)TAGATATCTCACATTCATAAGTACAAATCAGTCTGC BDL119 EcoRVFwd: BDL119_NF_EcRV (SEQ ID NO: 946) TTGATATCAGTTTCTCCGTCGACGATACCRev: BDL119_NR_EcRV (SEQ ID NO: 947) AAGATATCGGTCAAGTACATAAGCTAATAGATGBDL120 SacI, SalI Fwd: BDL120_F_SalI (SEQ ID NO: 948)AATGTCGACAACAATGGTGCTTCTACTTGTGATTG Rev: BDL120_R_SacI (SEQ ID NO: 949)TGAGCTCTCACTTCCACTAGTCACTACAAGCG BDL122 SalI, XbaIFwd: BDL122_NF_SI (SEQ ID NO: 950) CTGGTCGACACAGTATTGAGAGACTTCCTGGTGRev: BDL122_NR_Xba (SEQ ID NO: 951) GCTTCTAGACAATGTGAACTAAATCGACC BDL123SacI Fwd: BDL123_F_Sac (SEQ ID NO: 952) AGAGCTCGTTTTCTTCGCCATGGCRev: BDL123_R_Sac (SEQ ID NO: 953) TGAGCTCTTAAACAGTGACTACCACAGTGCABDL124 EcoRV Fwd Nested: BDL124_NF_EcRV (SEQ ID NO: 954)TCGATATCGGAATCAGAATCTTTTCAGATGGFwd External: BDL124_EF_EcRV (SEQ ID NO: 955)CTGATATCGAGTTTCTCTTCCTTAATTGTCGRev Nested: BDL124_NR_EcRV (SEQ ID NO: 956) TTGATATCATCATCAGCTTGGAACCTCGRev External: BDL124_ER_EcRV (SEQ ID NO: 957)TAGATATCTCTTTCCATCGATCATCAGC BDL125 SalI, XbaIFwd: BDL125_NF_SI (SEQ ID NO: 958) CTAGTCGACTAACAACAATGGAGAACCCTCRev: BDL125_NR_Xb (SEQ ID NO: 959) ACTCTAGATTAATGATCAACCAATTGGTCTTAGBDL127_GA SacI, XbaI Synthetic product BDL128 XbaIFwd: BDL128_NF_XbaI (SEQ ID NO: 960) TATCTAGAAGAAAATGGCGATGCGACRev: BDL128_NR_XbaI (SEQ ID NO: 961) TATCTAGATCATCACACATCCTGAGATACTTCATCBDL129_GA SacI, XbaI Synthetic product BDL130_GA SacI, XbaISynthetic product BDL131 SalI, XbaI Fwd: BDL131_NF_SalI (SEQ ID NO: 962)AATGTCGACAGAGAAATGTTGGCTATCTTCC Rev: BDL131_NR_XbaI (SEQ ID NO: 963)TATCTAGATCATCAGAGAGACCAATTGGCTTC BDL132 SalI, XbaIFwd Nested: BDL132_NF_SalI (SEQ ID NO: 964)AATGTCGACTTTGAATGGAACCATCATCTGFwd External: BDL132_EF_SalI (SEQ ID NO: 965)TTAGTCGACCTGAATCTGTTTTTGAATGGAACRev Nested: BDL132_NR_XbaI (SEQ ID NO: 966)TATCTAGATTATTAGGTGGAAAGAACAAGCGRev External: BDL132_ER_XbaI (SEQ ID NO: 967)TATCTAGATCAACAAGACAAGATAATGAAAGACACAG BDL133 EcoRVFwd: BDL133_NF_EcoRV(SEQ ID NO: 968) TAGATATCTTAAAATGCCGGAGAAAGGRev: BDL133_NR_EcoRV (SEQ ID NO: 969)ATGATATCCTACTATCTTACACACAATGCATTCAG BDL134_GA SacI, XbaISynthetic product BDL135 SalI, XbaI Fwd: BDL135_NF_SalI (SEQ ID NO: 970)ATAGTCGACGAAACATGGTTGAATCGGAC Rev: BDL135_NR_XbaI (SEQ ID NO: 971)TATCTAGATTAGACACTTTATGCCTCCTTTGTAG BDL136 SalI, SacIFwd: BDL136_NF_SI (SEQ ID NO: 972) AGCGTCGACTTAGAGAGAGATGCAGAAACGGRev: BDL136_NR_Sc (SEQ ID NO: 973) CGAGCTCCTAATCTAGAGAAGACTTTTACATGCCBDL137_GA SacI, XbaI Synthetic product BDL139_GA SacI, XbaISynthetic product BDL141_GA SacI, XbaI Synthetic product BDL142SalI, XbaI Fwd Nested: BDL142_NF_SalI (SEQ ID NO: 974)AATGTCGACCATCCTCATGAATAATTCTACATCFwd External: BDL142_EF_SalI (SEQ ID NO: 975)ACTGTCGACGCATTCCATTCATCCTCATGARev Nested: BDL142_NR_Xba (SEQ ID NO: 976)ATTCTAGAGTGTGATTATCAGTTTGTTCTCTCRev External: BDL142_ER_XbaI (SEQ ID NO: 977)ATTCTAGAGAAACGACAAGTGATTATAATGG BDL143 SalI, BamHIFwd: BDL143_F_SalI (SEQ ID NO: 978) ACTGTCGACAACATGTTGTTTAACTGGACTAAGRev Nested: BDL143_NR_BamHI (SEQ ID NO: 979)ATGGATCCTTACAGAACCGGTCAAGATGAAGRev External: BDL143_ER_BamHI (SEQ ID NO: 980)ATGGATCCCAATAACTCGAACACGAACAAC BDL144 EcoRVFwd: BDL144_F_EcoRV (SEQ ID NO: 981) TAGATATCAACAATGATTACAGTAGCCCCCTTCRev Nested: BDL144_NR_EcoRV (SEQ ID NO: 982)ATGATATCCTAACAAGCACAAGACTGATACAGCRev External: BDL144_ER_EcoRV (SEQ ID NO: 983)ATGATATCCAAAAGCTAGCTACTAGTTTCATCAC BDL145 SalI, XbaIFwd: BDL145_F_Sal (SEQ ID NO: 984) ATAGTCGACGAAAGAAAGAGAAAGCAGAACATGRev: BDL145_NR_XbaI (SEQ ID NO: 985) ATTCTAGATGGAGGAGCAAATACAAACTTGBDL146 SalI, XbaI Fwd: BDL146_F_SalI (SEQ ID NO: 986)AATGTCGACGAAACTTGGTTTTGAGCTTAACRev Nested: BDL146_NR_XbaI (SEQ ID NO: 987)ATTCTAGATCATCCCATTGCTTTCTCTAGTATTAGRev External: BDL146_ER_XbaI (SEQ ID NO: 988)ATTCTAGATTAAATGTATCGCTCCAAAAGAC BDL148 SalI, SacIFwd Nested: BDL148_NF_SalI (SEQ ID NO: 989)ACTGTCGACCTAATTCTCTCCGTCTCGATCGFwd External: BDL148_EF_SalI (SEQ ID NO: 990)ACTGTCGACGACTGATTTTACGCTTTATTGCTCRev Nested: BDL148_NR_NEW_SacI (SEQ ID NO: 991)GTGAGCTCTTAAACAGGTCATCTCGAGCCACRev External: BDL148_ER_NEW_SacI (SEQ ID NO: 992)GAGAGCTCCGTTGCCTGACAGAATCTTTG Table 13. Provided are the PCR primersused for cloning the genes described in Table 12 above. Fwd = forwardprimer; Rev = reverse primer; Nested = nested primer for PCR (internalprimer); External = external primer for PCR.

Sequencing of the amplified PCR products was performed, using ABI 377sequencer (Amersham Biosciences Inc). To facilitate cloning of thecDNAs/genomic sequences, a 8-12 bp extension was added to the 5′ of eachprimer. The primer extension includes an endonuclease restriction site.The restriction sites were selected using two parameters: (a). The sitedid not exist in the cDNA sequence; and (b). The restriction sites inthe forward and reverse primers were designed such that the digestedcDNA is inserted in the sense formation into the binary vector utilizedfor transformation.

PCR products were digested with the restriction endonucleases (NewEngland BioLabs Inc) according to the sites design in the primers (Table13, above) and cloned into binary vectors according to Table 14, below.RACE products were sequenced as described hereinbelow for BDL108, BDL110 and BDL111.

TABLE 14 Restriction enzyme sites used to clone the identified genesinto binary vector Restriction enzymes Restriction enzymes used forcloning into used for cloning into Restriction enzymes Binary binaryvector- binary vector- used for digesting Gene name vector FORWARDREVERSE the binary vector BDL42 pBXYN SaIl EcoRI SalI, EcoRI BDL46 pBXYNSalI SmaI SalI, Ecl136 BDL51 pBXYN SaIl EcoRI SalI, EcoRI BDL52 pBXYNSaIl EcoRI SalI, EcoRI BDL54 pBXYN SaIl EcoRI SalI, EcoRI BDL56 pBXYNSaIl EcoRI SalI, EcoRI BDL59 pBXYN SaIl EcoRI SalI, EcoRI BDL60 pBXYNSaIl EcoRI SalI, EcoRI BDL65 pBXYN SalI SmaI SalI, Ecl136 BDL67 pBXYNSalI SmaI SalI, Ecl136 BDL68 pBXYN SalI SmaI SalI, Ecl136 BDL78 pBXYNSaIl, EcoRI SalI, EcoRI BDL82 pBXYN SaIl EcoRI SaIl, EcoRI BDL89 pBXYNSaIl EcoRI SaIl, EcoRI BDL95 pBXYN SaIl EcoRI SaIl, EcoRI BDL100 pBXYNSalI SacI SalI, SacI BDL106 pBXYN SaIl EcoRI SaIl, EcoRI BDL108 pBXYNSaIl EcoRI SaIl, EcoRI BDL110 pBXYN SaIl EcoRI SaIl, EcoRI BDL111 pBXYNEcoRV EcoRV SmaI, Ecl136 BDL112 pBXYN SaIl EcoRI SaIl, EcoRI BDL114pBXYN SaIl EcoRI SaIl, EcoRI BDL115 pBXYN SmaI SacI SmaI, SacI BDL116pBXYN EcoRV EcoRV SmaI, Ecl136 BDL119 pBXYN EcoRV EcoRV SmaI, Ecl136BDL120 pBXYN SalI SacI SalI, SacI BDL122 pBXYN SaIl EcoRI SalI, EcoRIBDL123 pBXYN SaIl EcoRI SalI, EcoRI BDL124 pBXYN EcoRV EcoRV SmaI,Ecl136 BDL125 pBXYN SaIl EcoRI SaIl, EcoRI BDL128 pBXYN SaIl EcoRI SaIl,EcoRI BDL131 pBXYN SaIl EcoRI SaIl, EcoRI BDL132 pBXYN SaIl EcoRI SaIl,EcoRI BDL133 pBXYN EcoRV EcoRV SmaI, Ecl 136 II BDL135 pBXYN SaIl EcoRISaIl, EcoRI BDL136 pBXYN SaIl EcoRI SaIl, EcoRI BDL142 pBXYN SaIl EcoRISaIl, EcoRI BDL143 pBXYN BamHI Sal I SalI, Ecl 136 II BDL144 pBXYN EcoRVEcoRV SmaI, Ecl 136 II BDL145 pBXYN SaIl EcoRI SaIl, EcoRI BDL146 pBXYNSaIl EcoRI SaIl, EcoRI BDL148 pBXYN SaIl EcoRI SaIl, EcoRI BDL113_GApBXYN SacI Xba I SacI, Xba I BDL127_GA pBXYN SacI Xba I SacI, Xba IBDL129_GA pBXYN SacI Xba I SacI, Xba I BDL130_GA pBXYN SacI Xba I SacI,Xba I BDL134_GA pBXYN SacI Xba I SacI, Xba I BDL137_GA pBXYN SacI Xba ISacI, Xba I BDL139_GA pBXYN SacI Xba I SacI, Xba I BDL141_GA pBXYN SacIXba I SacI, Xba I Table 14.

Each digested PCR product was inserted into a high copy vectorpBlue-script KS plasmid vector [pBlue-script KS plasmid vector,stratagene (dot) com/manuals/212205 (dot) pdf] or into plasmidsoriginating from these vectors. In cases where the pGXN high copy vector(originated from pBlue-script KS) was used, the PCR product was insertedupstream to the NOS terminator (SEQ ID NO:776) originated from pBI 101.3binary vector (GenBank Accession No. U12640, nucleotides 4356 to 4693,SEQ ID NO:776) and downstream to the 35S promoter. In other cases(pKSJ_(—)6669a), the At6669 promoter (SEQ ID NO:775) was already clonedinto the pBlue-script KS so the gene was introduced downstream of thepromoter (Table 15 below). In all cases, after confirmation of thesequence of the cloned genes, the cloned cDNA accompanied or not withthe NOS terminator was introduced into the pGI binary vector [pBXYNcontaining the 35S CaMV promoter] according to Table 14, hereinabove,via digestion with an appropriate restriction endonucleases. In any casethe insert was followed by single copy of the NOS terminator (SEQ IDNO:776).

TABLE 15 Genes cloned from cDNA libraries or genomic DNA in a High copynumber plasmid High copy Amplified from Polynucleotide Polypeptide GeneName plasmid Organism Origin SEQ ID NO: SEQ ID NO: BDL42 pGXNArabidopsis mRNA 692 86 BDL46 pKS Arabidopsis mRNA 693 87 BDL51 pGXNArabidopsis mRNA 694 88 BDL52 pGXN Tomato mRNA 695 713 BDL54 pGXNArabidopsis mRNA 696 90 BDL56 pGXN Arabidopsis mRNA 697 91 BDL59 pGXNArabidopsis mRNA 698 92 BDL60 pGXN Arabidopsis mRNA 699 93 BDL65 pKSArabidopsis mRNA 700 94 BDL67 pKS Arabidopsis mRNA 701 714 BDL68 pKSArabidopsis mRNA 702 96 BDL78 pGXN Arabidopsis mRNA 703 97 BDL82 pGXNArabidopsis mRNA 704 98 BDL89 pGXN Rice mRNA 705 99 BDL95 pGXN Rice mRNA706 715 BDL100 pGXN Rice mRNA 657 707 BDL106 pGXN Canola mRNA 658 52BDL108 pGXN Canola mRNA 659 708 BDL110 pGXN Canola mRNA 660 709 BDL111pKSJ_6669a Canola mRNA 661 710 BDL112 pGXN Arabidopsis mRNA 662 56BDL114 pGXN Arabidopsis mRNA 664 58 BDL115 pKSJ Arabidopsis mRNA 665 59BDL116 pKSJ_6669a Arabidopsis mRNA 666 60 BDL119 pKSJ_6669a ArabidopsisGenomic 667 711 DNA BDL120 pGXN Arabidopsis mRNA 668 62 BDL122 pGXNArabidopsis mRNA 669 63 BDL123 pGXN Arabidopsis mRNA 670 64 BDL124pKSJ_6669a Arabidopsis mRNA 671 65 BDL125 pGXN Arabidopsis mRNA 672 66BDL128 pGXN Arabidopsis mRNA 674 68 BDL131 pGXN Arabidopsis mRNA 677 71BDL132 pGXN Arabidopsis mRNA 678 72 BDL133 pKSJ_6669a Arabidopsis mRNA679 73 BDL135 pGXN Arabidopsis mRNA 681 75 BDL136 pGXN Arabidopsis mRNA682 76 BDL142 pGXN Arabidopsis mRNA 686 80 BDL143 pKSJ Arabidopsis mRNA687 81 BDL144 pKSJ_6669a Arabidopsis mRNA 688 82 BDL145 pGXN ArabidopsismRNA 689 83 BDL146 pGXN Arabidopsis mRNA 690 84 BDL148 pGXN ArabidopsismRNA 691 85 BDL113_GA pGA4 Synthetic GeneArt 663 57 BDL127_GA pCR4Blunt-Synthetic GeneArt 673 67 TOPO BDL129_GA pGA4 Synthetic GeneArt 675 69BDL130_GA pGA14 Synthetic GeneArt 676 712 BDL134_GA pGA4 SyntheticGeneArt 680 74 BDL137_GA pGA18 Synthetic GeneArt 683 77 BDL139_GA pGA15Synthetic GeneArt 684 78 BDL141_GA pGA4 Synthetic GeneArt 685 79 Table15: Cloned and synthetic genes are provided along with the sequenceidentifiers of their polynucleotides and polypeptides. Also provided arethe source organism, tissue and the cloning vectors.

The digested products and the linearized plasmid vector were ligatedusing T4 DNA ligase enzyme (Roche, Switzerland). The plasmid pPI wasconstructed by inserting a synthetic poly-(A) signal sequence,originating from pGL3 basic plasmid vector (Promega, Acc No U47295; by4658-4811) into the HindIII restriction site of the binary vectorpBI101.3 (Clontech, Acc. No. U12640). pGI (FIG. 3) is similar to pPI,but the original gene in the backbone, the GUS gene, was replaced by theGUS-Intron gene followed by the NOS terminator (SEQ ID NO:776)(Vancanneyt. G, et al MGG 220, 245-50, 1990). pGI was used to clone thepolynucleotide sequences, initially under the control of 35S promoter[Odell, J T, et al. Nature 313, 810-812 (28 Feb. 1985); SEQ ID NO:777.

Selected DNA sequences were synthesized by a commercial supplierGeneArt, GmbH [geneart (dot) com/)]. Synthetic DNA is designed insilico. Suitable restriction enzymes sites were added to the clonedsequences at the 5′ end and at the 3′ end to enabled later cloning intothe pBXYN binary downstream of the CaMV 35S promoter (SEQ ID NO: 777).

Optimization of genes for expression in dicotyledonous plants—Tooptimize the coding sequence (in silico design), codon-usage Tablescalculated from plant transcriptions were used [example of such Tablescan be found in the Codon Usage Database available online at kazusa(dot) or (dot) jp/codon/]. The optimized coding sequences were designedin a way that no changes are introduced in the encoded amino acidsequence (of selected polypeptides from Table 1, Example 1) while usingcodons preferred for expression in dicotyledonous plants mainlyArabidopsis, Tomato, Canola and Soya while avoiding rare codons forArabidopsis; and monocotyledonous plants such as maize. Such optimizedsequences promote better translation rate and therefore higher proteinexpression levels. The genes for which codon optimized synthetic(artificial) sequences were prepared are: BDL-113 (SEQ ID NO:663polynucleotide, SEQ ID NO:57 polypeptide), BDL-127 (SEQ ID NO:673polynucleotide, SEQ ID NO:67 polypeptide), BDL-129 (SEQ ID NO:675polynucleotide, SEQ ID NO:69 polypeptide), BDL-130 (SEQ ID NO:676polynucleotide, SEQ ID NO:712 polypeptide), BDL-134 (SEQ ID NO:680polynucleotide, SEQ ID NO:74 polypeptide), BDL-137 (SEQ ID NO:683polynucleotide, SEQ ID NO:77 polypeptide), BDL-139 (SEQ ID NO:684polynucleotide, SEQ ID NO:78 polypeptide), BDL-141 (SEQ ID NO:685polynucleotide, SEQ ID NO:79 polypeptide).

Several polynucleotide sequences of the selected genes were cloneddownstream of the CaMV 35S promoter (SEQ ID NO:777), the ArabidopsisAt6669 promoter (SEQ ID NO:775) or the Napin seed specific promoter (SEQID NO:778).

The Napin (SEQ ID NO:778) promoter, which originates from Brassicanapus, is characterized by a seed specific promoter activity [Stuitje A.R. et. al. Plant Biotechnology Journal 1 (4): 301-309]. The Napinpromoter was amplified by direct PCR on genomic DNA extracted from leaftissue [using the DNAeasy kit (Qiagen Cat. No. 69104)] using thefollowing PCR primers: Napin F HindIII5′-ATAAGCTTATTGATTCCTTTAAAGACTTATGTT (SEQ ID NO:993) and Napin R SalI5′-TCGTCGACGGGTGTATGTTTTTAATCTTGTTT (SEQ ID NO:994). An example of agene cloned downstream of the Napin promoter sequence is BDL65 (SEQ IDNO:700).

For 9 genes, namely BDL52, BDL67, BDL95, BDL100, BDL108, BDL110, BDL111,BDL119 and BDL130, the protein translation of the amplified cDNAsequence did not match the initial bioinformatics prediction of theprotein sequences. The polypeptide sequences encoded by the cloned andtheir sequence identifiers are as follows: BDL52 (SEQ ID NO:713), BDL67(SEQ ID NO:714), BDL95 (SEQ ID NO:715), BDL100 (SEQ ID NO:707), BDL108(SEQ ID NO:708), BDL110 (SEQ ID NO:709), BDL111 (SEQ ID NO:710), BDL119(SEQ ID NO:711) and BDL130 (SEQ ID NO:712). Note that the BDL119 gene ispredicted to be a non-coding RNA (e.g., a regulatory RNA). The BDL119polynucleotide was cloned from a genomic DNA and BDL119 cDNA is providedin SEQ ID NO:667.

Example 5 Producing Transgenic Arabidopsis Plants Expressing theIdentified Polynucleotides of the Invention

Materials and Experimental Methods

Plant transformation—The Arabidopsis thaliana var Columbia (T₀ plants)were transformed according to the Floral Dip procedure [Clough S J, BentA F. (1998) Floral dip: a simplified method for Agrobacterium-mediatedtransformation of Arabidopsis thaliana. Plant J. 16(6): 735-43; andDesfeux C, Clough S J, Bent A F. (2000) Female reproductive tissues arethe primary targets of Agrobacterium-mediated transformation by theArabidopsis floral-dip method. Plant Physiol. 123(3): 895-904] withminor modifications. Briefly, Arabidopsis thaliana Columbia (Col0) T₀plants were sown in 250 ml pots filled with wet peat-based growth mix.The pots were covered with aluminum foil and a plastic dome, kept at 4°C. for 3-4 days, then uncovered and incubated in a growth chamber at18-24° C. under 16/8 hours light/dark cycles. The T_(o) plants wereready for transformation six days before anthesis.

Single colonies of Agrobacterium carrying the binary vectors harboringthe seed oil genes were cultured in LB medium supplemented withkanamycin (50 mg/L) and gentamycin (50 mg/L). The cultures wereincubated at 28° C. for 48 hours under vigorous shaking and centrifugedat 4000 rpm for 5 minutes. The pellets comprising Agrobacterium cellswere resuspended in a transformation medium which containedhalf-strength (2.15 g/L) Murashige-Skoog (Duchefa); 0.044 μM benzylaminopurine (Sigma); 112 μg/L B5 Gambourg vitamins (Sigma); 5% sucrose; and0.2 ml/L Silwet L-77 (OSI Specialists, CT) in double-distilled water, atpH of 5.7.

Transformation of T₀ plants was performed by inverting each plant intoan Agrobacterium suspension such that the above ground plant tissue wassubmerged for 3-5 seconds. Each inoculated T₀ plant was immediatelyplaced in a plastic tray, then covered with clear plastic dome tomaintain humidity and was kept in the dark at room temperature for 18hours to facilitate infection and transformation. Transformed(transgenic) plants were then uncovered and transferred to a greenhousefor recovery and maturation. The transgenic T₀ plants were grown in thegreenhouse for 3-5 weeks until siliques were brown and dry, then seedswere harvested from plants and kept at room temperature until sowing.

For generating T₁ and T₂ transgenic plants harboring the genes, seedscollected from transgenic T₀ plants were surface-sterilized by soakingin 70% ethanol for 1 minute, followed by soaking in 5% sodiumhypochlorite and 0.05% triton for 5 minutes. The surface-sterilizedseeds were thoroughly washed in sterile distilled water then placed onculture plates containing half-strength Murashig-Skoog (Duchefa); 2%sucrose; 0.8% plant agar; 50 mM kanamycin; and 200 mM carbenicylin(Duchefa). The culture plates were incubated at 4° C. for 48 hours thentransferred to a growth room at 25° C. for an additional week ofincubation. Vital T₁ Arabidopsis plants were transferred to a freshculture plates for another week of incubation. Following incubation theT₁ plants were removed from culture plates and planted in growth mixcontained in 250 ml pots. The transgenic plants were allowed to grow ina greenhouse to maturity. Seeds harvested from T₁ plants were culturedand grown to maturity as T₂ plants under the same conditions as used forculturing and growing the T₁ plants.

Example 6 Identification of Novel Promoters

Constitutive promoters allow continuous expression of genes regulatedthereby throughout the plant. A widely used example for a constitutivepromoter is the CaMV35S promoter from cauliflower mosaic virus (SEQ IDNO:777).

One of the important requirements for an engineered plant is to activatethe gene-of-interest in the right tissue or organ, and/or at theappropriate time (e.g., a certain developmental stage, under certainenvironmental conditions). For example, in order to influence a uniquetissue such as seed, the gene-of-interest may be induced for expression(activated) at a certain developmental stage such as pre embryofertilization, post fertilization, early or late embryogenesis. Forexample, to improve yield and/or oil content of a plant, the expressionof the gene-of-interest may be regulated by a promoter having anexpression pattern appropriate for seed development. Thus, thecombination of a target gene with specific promoters such asdevelopmental specific promoter (such as seed, carpel, stem, seedling)may increase the desired effect of the gene (e.g., improve yield and/oroil content) and may avoid undesired influence of the gene on otherbiological processes in other tissues, for example, cell structure,plant architecture.

The present inventors have isolated and validated novel developmentalspecific promoters from different stages of plant development and/orplant tissues, having different levels of gene expression. The followingdescription summarizes the process of selection and cloning of the novelArabidopsis promoters.

Cloning and analysis of promoters—The novel Arabidopsis promoters of theinvention were selected based on the expression profile of the nativegenes positioned downstream (3′) to the promoter sequences (see Table16, below).

TABLE 16 Expression profile based on microarray analysis Gene 3′ to thepromoter AT1G30860 AT2G31160 AT2G39640 AT3G21380 AT3G24510 AT3G61040AT4G15975 Description expressed Protein of Glycosyl similar to Encodes acytochrome Zinc of gene protein unknown hydrolase jacalin lectindefensin- P450 finger, product function family family protein likefamily C3HC4 (DUF640) 17 [Arabidopsis (DEFL) protein type proteinthaliana] family (RING (TAIR: At1g52040.1) protein finger) Specificity/Seed Stem Seed Seed Carpel Seed Seedling normalized expression levelcarpels 28.89 6.89 35.03 50.33 462.3 23.75 16.29 cauline 58.82 26.0618.62 8.48 4 40.36 4 cotyledons 27.56 35.91 30.1 4 4.59 36.86 39.32flower 21.59 97.92 35.48 32.46 23.73 26.94 18.92 hypocotyl 48.59 782.830.62 31.39 6.32 27.57 31.21 inflorescence 23.93 643.83 24.23 39.18 432.95 4 leaf 9.61 41.3 25 7.73 5.95 42.8 23.3 pedicels 4 12.22 22.7813.35 4.12 39.68 20.16 petals 4 16 41.7 14 4.48 36.9 17.2 petiole 4174.5 15.23 20.1 4 55.57 28.04 pollen 56.49 16.3 36.44 19.85 4 16.3120.17 root 20 91.6 39 38.2 6.91 35.8 19.3 rosette 8.19 37.84 26.28 17.494.78 33.78 27.05 seed 1172.9 52.49 30.41 1702.9 10.89 1186.5 24.34seedling 16.78 65.72 30.6 19.9 6.92 37.95 81.98 sepals 77.8 19.3 30 9.094 53.9 13.2 shoot 19.22 631.3 26.5 34.29 4.84 26.34 21.36 siliques 61.3660.56 132.9 16.83 698.8 39.27 22.16 stamen 66.15 17.04 32.04 62.88 420.98 21.67 stem 24.18 449.5 18.17 15.99 6.77 38.93 5.62 Table 16.Provided are the results of a microarray expression profile of genes(GenBank Accession NOs.) positioned 3′ of the identified promoters.Shown are the tissue specifcity of the promoters and the normalizedexpression levels of each gene in the specific tissue.

Table 17, hereinbelow, provides the sequence identifiers of the novelpromoters of the invention, along with the sequence identifiers of thegenes and the polypeptides encoded thereby positioned downstream of thenovel promoters of the invention.

TABLE 17 Identification of novel promoters The polynucleotides Thepolypeptides (GenBank (GenBank Accession Nos. and Accession Nos. and SEQID NO:) Promoter SEQ ID NO:) positioned encoded by the polynucleotidesdesignation downstream of the identified positioned downstream of thePromoter (SEQ ID NO:) promoters identified promoters length (bp) PrBDL40L AT1G30860 (SEQ ID AT1G30860_P1 SEQ ID NO: 800 2970 (SEQ ID NO: 793)NO: 779) PrBDL40 S AT1G30860 (SEQ ID AT1G30860_P1 SEQ ID NO: 800 2238(SEQ ID NO: 793) NO: 780) PrBDL34 L AT2G31160 (SEQ ID AT2G31160_P1 SEQID NO: 801 3097 (SEQ ID NO: 794) NO: 781) PrBDL34 S AT2G31160 (SEQ IDAT2G31160_P1 SEQ ID NO: 801 3000 (SEQ ID NO: 794) NO: 782) PrBDL36 LAT2G39640 (SEQ ID AT2G39640_P1 SEQ ID NO: 802 2889 (SEQ ID NO: 795) NO:783) PrBDL36 S AT2G39640 (SEQ ID AT2G39640_P1 SEQ ID NO: 802 831 (SEQ IDNO: 795) NO: 784) PrBDL38 L AT3G21380 (SEQ ID AT3G21380_P1 SEQ ID NO:803 3000 (SEQ ID NO: 796) NO: 785) PrBDL38 S AT3G21380 (SEQ IDAT3G21380_P1 SEQ ID NO: 803 880 (SEQ ID NO: 796) NO: 786) PrBDL37 LAT3G24510 (SEQ ID AT3G24510_P1 SEQ ID NO: 804 3000 (SEQ ID NO: 797) NO:787) PrBDL37 S AT3G24510 (SEQ ID AT3G24510_P1 SEQ ID NO: 804 1423 (SEQID NO: 797) NO: 788) PrBDL39 L AT3G61040 (SEQ ID AT3G61040_P1 SEQ ID NO:805 3000 (SEQ ID NO: 798) NO: 789) PrBDL39 S AT3G61040 (SEQ IDAT3G61040_P1 SEQ ID NO: 805 1159 (SEQ ID NO: 798) NO: 790) PrBDL35 LAT4G15975 (SEQ ID AT4G15975_P1 SEQ ID NO: 806 2881 (SEQ ID NO: 799) NO:791) PrBDL35 S AT4G15975 (SEQ ID AT4G15975_P1 SEQ ID NO: 806 942 (SEQ IDNO: 799) NO: 792) Table 17. Provided are the identified promoters, theirlength and sequence identifiers along with the genes found downstream tothe promoters.

Construction of promoter::GUS fusion nucleic acid construct for analysisof expression pattern of the identified promoters—For cloning of each ofthe promoter sequences two sets of primers that span the predictedpromoter sequence were designed. The short sequence of the promoter wasamplified using a 3′ primer sequence selected near the start codon ofthe coding sequence of the downstream gene (which is located downstreamto the promoter sequences) and a 5′ primer sequence selected from thesequence that is downstream to the adjacent upstream gene. The longsequence of the promoter was using a 3′ primer sequence selected fromthe start of the untranslated region (5′UTR) of the gene downstream ofthe promoter and a 5′ primer sequence located 3 kb upstream of the 3′primer (See Table 18, below). Each promoter sequences wastranslationally fused to the GUS coding sequence (a reporter gene).

All sequences were amplified by PCR. The PCR products were purifiedusing MINELUTE® (QIAGEN GmbH) PCR purification kit (Qiagen) andsequencing of the amplified PCR products was performed, using ABI 377sequencer (Applied Biosystems). To facilitate cloning of the promotersequences, a 8-12 bp extension was added to the 5′ of each primer. Theprimer extension includes an endonuclease restriction site. Therestriction sites are selected using two parameters: a.) The site doesnot exist in the promoter sequence. b.) The restriction sites in theforward and reverse primers are designed so the digested genomic DNA isinserted in the sense formation into the binary vector utilized fortransformation. For instance, the pGI plasmid vector was constructed byinserting a synthetic poly-(A) signal sequence, originating from pGL3basic plasmid vector (Promega, Acc. No. U47295; by 4658-4811) into theHindIII restriction site of the binary vector pBI101.3 (Clontech, Acc.No. U12640) and GUS-Intron gene (Vancanneyt. G, et al MGG 220, 245-50,1990). Another plasmid vector used for cloning was the pMBLArt (Gleave AP. Plant Mol Biol. 1992 December; 20(6): 1203-7).

The digested PCR products were first subcloned into pBlue-script KS[(originated from the pBlue-script KS plasmid vector stratagene (dot)com/manuals/212205 (dot) pdf)] followed by cloning into pGI binaryvector with the GUS-Intron gene (Vancanneyt. G, et al MGG 220, 245-50,1990) and the NOS terminator originated from pBI 101.3 binary vector(GenBank Accession No. U12640; GI:529333 nucleotides 4356 to 4693, SEQID NO:776). Some of the PCR products were first sublcloned pBlue-scriptKS [(originated from the pBlue-script KS plasmid vector stratagene (dot)com/manuals/212205 (dot) pdf)] with the GUS-Intron gene (Vancanneyt. G,et al MGG 220, 245-50, 1990) and the NOS terminator originated from pBI101.3 binary vector followed by cloning the entire cassette into thebinary vector pMBLArt (according to Table 19). The digested PCR productand the linearized plasmid vector were ligated using T4 DNA ligaseenzyme (Roche, Switzerland). The primers used for cloning are providedin Table 18.

TABLE 18 Primers used for the cloning of the novel promoters RestrictionEnzymes used Primers used for amplification/ Promoter Name for cloningSEQ ID NO: PrBDL34_L PstI, SalI Fwd: PrBDL34_EF_PstI-ATCTGCAGGAAATTGGAAAAGGGTTTAAC/ SEQ ID NO: 995 Rev: PrBDL34_ER_SalI-ATTGTCGACGATTAGTTCTTGATTCTTGATCTTTC/ SEQ ID NO: 996 PrBDL35 LHindIII, SalI Fwd: PrBDL_35_F_HindIII-ATAAAGCTTCATTGACTTGAGATTCAGTTCATG/ SEQ ID NO: 997 Rev: PrBDL_35_R_SalI-ATTGTCGACAGAGAAGTGAATGAAGATTTTAGG/ SEQ ID NO: 98 PrBDL36 L SalI, XbaIFwd: PrBDL_36_F_SalI- AATGTCGACCGAATCAATACGTAACTTTCAATC/ SEQ ID NO: 999Rev: PrBDL_36_R_XbaI- TATCTAGATGCTTTGTTTTGTTTTGTTTTG/ SEQ ID NO: 1000PrBDL37_S HindIII, SalI Fwd: PrBDL37_Short_F_HindIII-ACTAAGCTTGACTTGATACTAACGAGGAAATG/ SEQ ID NO: 001Rev: PrBDL37_Short_R_SalI- TGTGTCGACTTTCAAATTTTTAGAATGGGAG/SEQ ID NO: 1002 PrBDL38_S PstI, SalI Fwd: PrBDL38_Short_Fl_PstI-AACTGCAGAGCTCACGAGTGTGTTTTTGG/ SEQ ID NO: 1003Rev: PrBDL38_Short_R_SalI- ATTGTCGACTGTATCTGATCATATCTTACCGG/SEQ ID NO: 1004 PrBDL39 L HIndIII, SmaI Fwd: PrBDL_39_F_HindIII-ATTAAGCTTCCTGCAACAATGATTTATTATG/ SEQ ID NO: 1005 Rev: PrBDL_39_R_SmaI-TCCCGGGCTAATATTATGCACGCTTCGTC/ SEQ ID NO: 1006 PrBDL40 L HindIII, SalIFwd: PrBDL_40_F_HindIII- TATAAGCTTCATCTCGGACTTGATATCGTC/ SEQ ID NO: 1007Rev: PrBDL_40_R_SalI-1- ATTGTCGACGAATCGAACAAACGAACATAAA/ SEQ ID NO: 1008Table 18.

Table 19, hereinbelow, provides the cloning vectors used to clone eachof the identified promoters.

TABLE 19 Promoters cloned into different binary vectors Promoter ClonedIn pGI Cloned In pMBLArt PrBDL40 L V PrBDL34 L V PrBDL36 L V PrBDL38 S VPrBDL37 S V PrBDL39 L V PrBDL35 L V Table 19: Provided are the promoterdesignations (sequence identifiers are given in Table 17, above) and thevectors used for their cloning. “V” indicates that the promoter wascloned in the noted vector.

Constructs were transformed into Arabidopsis plants as described inExample 5 above and expression analysis based on the monitoring theexpression level of the GUS gene (GUS staining) was performedessentially as described in Jefferson R A. et. al. 1987 EMBO J 6 (13),3901-3907; and Meissner et. al. 2000 Plant Journal 22 (3), 265-274.

The level of GUS staining was determined according to the intensity ofthe blue color. Table 20, below, provides the coloring level of GUSstaining.

TABLE 20 Coloring level no color 0 medium − 2 medium dark 3 medium + 4darkest 5 Table 20: The index of blue color intensity.

Table 21, hereinbelow, describes the expression pattern of the clonedpromoters.

TABLE 21 Expression pattern of developmental stage promoters PromoterSEQ ID Small Large Promoter NO: Event flowers flowers Leaves StalkSiliques pMBL_GI 777 5681.1 4-5 2 3 2-5 2-5 pMBL_GI 777 5681.2 4-5 2 2-32-5 3-5 pMBL_GI 777 5681.3 4-5 2 3 2-5 2-5 pMBL_GI 777 5681.4 4-5 2 33-5 3-5 pMBL_GI 777 5681.5 4-5 2 3 2-5 2-5 pM_PrBDL40_YN 779 6552.1 0 01 1-3 1 pM_PrBDL40_YN 779 6552.2 0 0 1 0-2 0 pM_PrBDL40_YN 779 6552.3 33 3 3 3 pM_PrBDL40_YN 779 6552.4 0 5 1 5 0 pM_PrBDL40_YN 779 6554.1 0 00 0-1 0 pM_PrBDL40_YN 779 6554.2 1 4 0-5 3 0 pM_PrBDL40_YN 779 6554.3 04 0-5 5 0 pM_PrBDL40_YN 779 6554.4 0 1-2 0-2 0 0 pM_PrBDL39_YN 7896501.1 0 0 0 0 0 pM_PrBDL39_YN 789 6501.2 1 0 0 0 0 pM_PrBDL39_YN 7896501.3 0 0 0 0 0 pM_PrBDL39_YN 789 6501.4 0 0 0 0 0 pM_PrBDL39_YN 7896501.5 0 0 0 0 0 pM_PrBDL39_YN 789 6502.1 0 0 0 0 0 pM_PrBDL39_YN 7896502.2 0 0 0 0 0 pM_PrBDL39_YN 789 6502.3 0 0 0 0 0 pM_PrBDL39_YN 7896502.4 0 0 0 0 0 pM_PrBDL39_YN 789 6502.5 0 0 0 0 0 pM_PrBDL35_YN 7916512.1 0 0 0 0 0 pM_PrBDL35_YN 791 6512.2 0 0 0 0 0 pM_PrBDL35_YN 7916512.3 0 0 0 0 3 pM_PrBDL35_YN 791 6512.4 0 0 0 0 0 pM_PrBDL35_YN 7916512.5 0 0 0 0 0 pM_PrBDL35_YN 791 6511.1 0 0 0 0 0 pM_PrBDL35_YN 7916511.2 0 0 0 0 3-5 pM_PrBDL35_YN 791 6511.3 0 0 0 0 1-2 pM_PrBDL35_YN791 6511.4 0 0 0 0 0 pM_PrBDL35_YN 791 6511.5 0 0 0 0 0 WT 0 0 0 0 0Table 21. “pM” or “pMBL” refer to the binary vector pMBLArt whichincludes the CaMV35S promoter (SEQ ID NO: 777). “Y” or “GI” refer to GUSintron. “N” refers to NOS terminator. pMBL_GI serves as a positivecontrol. PrBDL40 = SEQ ID NO: 779 (L); PrBDL39 = SEQ ID NO: 789 (L));PrBDL35 = SEQ ID NO: 791 (L)

These results demonstrate that the novel promoters of the invention arecapable of directing expression of a heterologous polynucleotide in ahost cell in a tissue specific and/or developmental stage-specificmanner.

Example 7 Improved Transgenic Plant Performance

To analyze the effect of expression of the isolated polynucleotides inplants, plants were grown in pots with an adequate amount of nutrientsand water. The plants were analyzed for their overall size, growth rate,time to inflorescence emergence (bolting) and flowering, seed yield,weight of 1,000 seeds, dry matter and harvest index [(HI) seed yield/drymatter]. Transgenic plants performance was compared to control plantsgrown in parallel under the same conditions. Mock-transgenic plants withan empty vector or expressing the uidA reporter gene (GUS-Intron) underthe same promoter were used as control.

Parameters were measured as described in Example 3 above.

Statistical analyses—Plant growth rate, plant area, time to bolt, timeto flower, weight of 1,000 seeds, seed yield, oil yield, dry matter, andharvest index area data were analyzed using t-test. To identifyoutperforming genes and constructs, results from mix of transformationevents or independent events were analyzed. For gene versus controlanalysis t-test was applied, using significance of p<0.1. The JMPstatistics software package was used (Version 5.2.1, SAS Institute Inc.,Cary, N.C., USA).

Experimental Results

Plants expressing the polynucleotides of the invention were assayed fora number of commercially desired traits. Table 22 provides theparameters measured in a tissue culture assay (results are presented inTable 23).

TABLE 22 Parameter symbol used in result Table 23 Parameter name 1 LeafArea time point 1 2 Leaf Area time point 2 3 Leaf Area time point 3 4Roots Length time point 1 5 Roots Length time point 2 6 Roots Lengthtime point 3 7 Roots Coverage time point 1 8 Roots Coverage time point 29 Roots Coverage time point 3 10 RGR of Leaf Area time point 2 11 RGR ofLeaf Area time point 3 12 RGR of Roots Coverage time point 2 13 RGR ofRoots Coverage time point 3 14 RGR of Roots Length time point 2 15 RGRof Roots Length time point 3 16 Fresh Weight 17 Dry Weight Table 22. RGR= relative growth rate.

Analysis of plants in tissue culture assay—Table 23, hereinbelow,depicts analyses of seed yield in plants overexpressing thepolynucleotides of the invention under the regulation of theconstitutive 35S (SEQ ID NO:777) or At6669 (SEQ ID NO:775) promoters. Incases where a certain event appears more than once, the event was testedin several independent experiments.

TABLE 23 Results obtained in a tissue culture assay Gene Ev. Par. 1 2 34 5 6 7 8 9 10 11 12 13 14 15 16 17 BDL100 7872.2 P 0.10 0.07 0.34 0.17BDL100 7872.2 Av 1.15 1.77 1.24 1.40 BDL100 7872.3 P 0.22 0.19 0.01BDL100 7872.3 Av 1.10 1.46 1.63 BDL100 7873.2 P 0.16 0.75 0.02 0.51 0.01BDL100 7873.2 Av 1.12 1.13 1.94 1.17 1.75 BDL100 7873.3 P 0.24 0.09 0.01BDL100 7873.3 Av 1.16 1.47 1.76 BDL100 7873.4 P 0.20 0.06 0.16 0.01 0.06BDL100 7873.4 Av 1.12 1.25 1.22 1.17 1.12 BDL108 8122.1 P 0.20 0.24 0.500.11 BDL108 8122.1 Av 1.38 1.35 1.32 1.58 BDL108 8122.2 P 0.09 0.09 0.12BDL108 8122.2 Av 1.83 1.47 1.27 BDL108 8123.5 P 0.77 0.32 BDL108 8123.5Av 1.13 1.31 BDL108 8123.6 P BDL108 8123.6 Av BDL110 8092.1 P 0.05 0.410.13 0.42 0.31 0.31 BDL110 8092.1 Av 1.23 1.13 1.39 1.16 1.15 1.33BDL110 8092.2 P 0.35 0.22 0.13 0.18 0.04 BDL110 8092.2 Av 1.22 3.01 1.252.69 1.33 BDL110 8092.5 P 0.00 0.01 0.00 0.01 0.18 0.16 0.11 0.33 0.01BDL110 8092.5 Av 1.86 1.30 1.53 1.40 2.04 1.58 2.58 1.11 2.24 BDL1108095.2 P 0.00 0.05 0.22 0.00 0.03 0.10 BDL110 8095.2 Av 1.53 1.47 1.231.62 1.40 1.56 BDL110 8722.3 P 0.07 0.01 0.22 0.05 0.06 0.16 BDL1108722.3 Av 1.36 2.31 1.14 2.47 1.28 1.47 BDL114 7741.3 P 0.07 0.66 0.010.01 0.02 0.15 BDL114 7741.3 Av 1.51 1.17 1.84 1.51 1.75 1.37 BDL1147741.6 P 0.24 0.34 0.64 0.00 0.25 BDL114 7741.6 Av 1.14 1.22 1.13 1.401.23 BDL114 7742.1 P 0.36 0.26 0.19 0.62 0.14 0.06 0.51 BDL114 7742.1 Av1.12 1.15 1.32 1.14 1.29 1.49 1.16 BDL114 7742.3 P 0.17 0.69 0.08 0.090.08 0.01 BDL114 7742.3 Av 1.28 1.24 2.68 2.00 2.89 1.80 BDL114 7742.5 P0.24 0.37 0.31 0.06 0.59 0.06 BDL114 7742.5 Av 1.22 1.40 1.47 2.31 1.272.05 BDL116 7481.2 P 0.32 0.06 0.16 0.04 BDL116 7481.2 Av 1.34 1.59 1.271.14 BDL116 7481.7 P 0.03 0.21 0.01 0.07 BDL116 7481.7 Av 1.85 1.44 2.111.59 BDL116 7481.8 P 0.30 0.13 0.24 0.59 0.30 0.42 0.27 0.36 0.62 0.17BDL116 7481.8 Av 1.25 1.40 1.26 1.15 1.34 1.26 1.16 1.17 1.12 1.20BDL116 7482.2 P 0.46 0.62 0.22 0.35 0.02 0.17 BDL116 7482.2 Av 1.11 1.161.40 1.56 1.44 1.58 BDL116 7485.1 P 0.21 0.26 0.20 0.03 0.10 BDL1167485.1 Av 1.27 1.78 1.47 2.06 1.44 BDL120 7891.3 P 0.12 0.07 0.01 0.01BDL120 7891.3 Av 1.35 1.41 1.63 1.52 BDL120 7892.4 P 0.28 0.42 0.04 0.150.02 0.03 BDL120 7892.4 Av 1.15 1.12 2.33 1.44 2.49 1.70 BDL120 7892.6 P0.03 0.77 0.02 BDL120 7892.6 Av 1.63 1.12 1.44 BDL120 7893.2 P 0.36 0.170.57 0.06 0.28 BDL120 7893.2 Av 1.27 1.96 1.14 1.86 1.26 BDL120 7893.5 P0.30 0.45 BDL120 7893.5 Av 1.73 1.49 BDL123 8082.1 P 0.05 0.54 0.07 0.170.11 BDL123 8082.1 Av 1.37 1.19 1.38 1.58 1.59 BDL123 8082.3 P 0.27 0.120.33 0.01 0.43 BDL123 8082.3 Av 1.64 1.75 1.41 1.85 1.18 BDL123 8082.6 P0.03 0.07 0.07 0.01 BDL123 8082.6 Av 1.67 1.52 2.05 1.62 BDL123 8083.2 P0.07 0.35 0.45 0.31 0.07 BDL123 8083.2 Av 1.25 1.62 1.50 1.67 1.86BDL123 8083.3 P 0.20 0.72 0.11 0.34 0.18 BDL123 8083.3 Av 1.34 1.12 1.571.28 1.51 BDL125 7491.1 P 0.30 0.36 0.26 BDL125 7491.1 Av 1.16 1.46 1.38BDL125 7491.5 P 0.16 0.03 0.43 0.01 BDL125 7491.5 Av 1.24 2.32 1.28 2.15BDL125 7492.5 P 0.29 0.03 0.22 0.00 0.04 BDL125 7492.5 Av 1.22 2.17 1.542.83 1.61 BDL125 7494.1 P 0.04 0.07 0.05 0.14 0.30 0.08 0.23 BDL1257494.1 Av 1.21 1.20 1.41 1.43 1.81 2.60 1.45 BDL125 7495.5 P 0.36 0.510.05 0.09 0.05 0.00 BDL125 7495.5 Av 1.12 1.10 3.54 1.45 3.05 1.82BDL128 7711.3 P 0.37 BDL128 7711.3 Av 1.35 BDL128 8361.5 P 0.13 0.080.10 0.09 BDL128 8361.5 Av 1.74 1.43 1.91 1.66 BDL128 8362.2 P 0.32 0.180.35 BDL128 8362.2 Av 1.17 1.31 1.36 BDL128 8363.2 P 0.04 0.30 0.05 0.150.00 BDL128 8363.2 Av 1.24 1.37 1.46 1.83 1.46 BDL128 8365.2 P 0.02 0.010.11 BDL128 8365.2 Av 1.70 3.04 2.64 BDL129 7691.2 P 0.13 0.40 0.35 0.540.63 0.00 0.18 0.03 0.52 0.00 0.09 BDL129 7691.2 Av 1.40 1.30 1.34 1.271.14 2.99 1.77 2.10 1.17 1.83 1.73 BDL129 7692.2 P 0.10 0.16 0.01 0.000.03 0.00 0.03 0.09 BDL129 7692.2 Av 1.16 1.29 3.43 1.85 1.82 1.71 1.851.42 BDL129 7692.5 P 0.03 0.09 0.08 BDL129 7692.5 Av 1.37 1.71 1.61BDL129 7693.1 P 0.51 0.00 0.27 0.00 0.27 BDL129 7693.1 Av 1.37 2.05 1.581.82 1.52 BDL129 7693.4 P 0.07 0.09 0.05 0.13 BDL129 7693.4 Av 2.43 1.831.69 1.60 BDL130 7661.7 P 0.06 0.01 0.00 0.00 0.02 0.12 0.04 0.01 0.010.27 BDL130 7661.7 Av 1.22 1.14 1.32 1.45 3.08 1.79 2.15 1.58 1.88 1.23BDL130 7663.1 P 0.03 0.25 0.00 0.00 0.00 0.67 BDL130 7663.1 Av 1.24 1.201.40 1.41 1.53 1.10 BDL130 7663.3 P 0.06 0.42 0.01 0.62 0.01 BDL1307663.3 Av 1.60 1.29 2.93 1.14 1.96 BDL130 7663.6 P 0.12 0.03 0.08 0.040.00 BDL130 7663.6 Av 1.21 2.28 1.75 1.98 1.79 BDL130 7664.5 P 0.33 0.030.00 0.10 0.07 0.26 0.00 0.02 0.00 0.00 0.51 BDL130 7664.5 Av 1.11 1.351.35 1.16 1.19 1.11 1.31 1.33 1.37 1.42 1.10 BDL130 8572.4 P 0.01 0.070.07 0.23 0.05 0.00 0.10 0.00 0.00 0.01 BDL130 8572.4 Av 1.47 1.18 1.291.20 2.51 2.92 1.72 2.16 2.33 2.68 BDL130 8573.5 P 0.35 0.08 0.32 0.150.29 0.28 0.09 0.09 0.04 0.18 BDL130 8573.5 Av 1.12 1.21 1.13 1.28 1.221.30 1.91 1.33 1.36 1.24 BDL130 8574.2 P 0.39 0.46 0.23 0.28 0.05 0.060.16 0.10 BDL130 8574.2 Av 1.17 1.15 1.28 1.44 1.84 1.64 1.37 1.60BDL130 8574.4 P 0.03 0.05 0.01 0.37 0.13 0.05 0.17 0.03 0.02 0.14 0.610.55 0.05 0.05 BDL130 8574.4 Av 1.46 1.50 1.65 1.40 1.35 1.32 1.98 2.002.05 1.25 1.12 1.14 2.26 2.30 BDL130 8575.1 P 0.00 0.00 0.00 0.11 0.080.00 0.03 0.02 0.13 0.03 0.13 0.00 0.31 BDL130 8575.1 Av 1.44 1.51 1.561.21 1.28 1.50 1.94 1.88 1.32 1.35 1.20 2.07 1.59 BDL131 8631.1 P 0.020.40 0.06 0.05 0.00 BDL131 8631.1 Av 1.36 1.23 1.77 1.82 1.65 BDL1318632.2 P 0.01 0.01 0.01 0.24 0.15 0.25 0.03 0.03 0.20 0.00 0.03 0.05BDL131 8632.2 Av 1.61 1.64 1.66 1.20 1.19 1.30 1.60 2.05 1.58 1.65 3.332.82 BDL131 8633.2 P 0.14 0.39 0.48 0.05 0.14 0.11 0.08 BDL131 8633.2 Av1.12 1.21 1.22 2.07 1.20 1.38 1.74 BDL131 8634.2 P 0.00 0.13 0.08 0.130.22 0.03 0.00 0.00 0.01 0.00 0.00 BDL131 8634.2 Av 1.34 1.23 1.35 1.271.23 2.70 2.39 2.04 1.54 2.04 2.49 BDL131 8635.4 P 0.00 0.15 0.23 0.080.12 0.18 0.12 0.09 0.11 0.49 0.30 0.27 0.51 0.05 0.03 BDL131 8635.4 Av1.24 1.41 1.70 1.14 1.26 1.28 1.35 1.77 1.79 1.20 1.34 1.41 1.17 1.682.07 BDL133 8542.2 P 0.45 0.47 0.52 BDL133 8542.2 Av 1.10 1.11 1.20BDL133 8542.3 P 0.04 0.02 0.02 0.00 0.02 0.01 0.05 0.21 0.01 0.16 0.010.03 0.10 0.00 BDL133 8542.3 Av 1.41 1.72 1.30 1.47 1.84 2.02 1.48 1.531.95 1.24 1.45 1.42 1.69 1.85 BDL133 8543.4 P 0.16 0.10 0.17 0.59 0.170.17 0.01 0.41 0.00 0.09 0.15 BDL133 8543.4 Av 1.21 1.36 1.43 1.18 1.181.39 1.91 1.25 1.58 2.03 1.73 BDL133 8544.3 P 0.22 0.26 0.17 0.14 0.040.51 0.23 0.07 0.30 0.26 0.31 0.35 0.29 0.38 0.17 BDL133 8544.3 Av 1.321.23 1.30 1.16 1.20 1.24 1.39 1.51 1.15 1.21 1.28 1.18 1.17 1.21 1.46BDL133 8545.3 P 0.07 0.01 0.00 0.51 0.24 0.18 0.07 0.51 0.05 0.04 0.040.17 0.00 0.00 BDL133 8545.3 Av 1.51 1.68 1.63 1.10 1.18 1.44 1.81 1.322.76 1.55 2.12 1.26 2.51 3.55 BDL134 7671.2 P 0.69 0.61 0.21 0.07 BDL1347671.2 Av 1.12 1.17 1.30 1.42 BDL134 7672.5 P 0.33 0.63 0.24 0.08 0.200.04 BDL134 7672.5 Av 1.16 1.11 1.27 1.47 1.56 1.74 BDL134 7673.1 P 0.400.12 0.00 0.43 BDL134 7673.1 Av 1.13 1.29 1.74 1.24 BDL134 7673.2 P 0.440.00 0.03 BDL134 7673.2 Av 1.33 2.67 2.32 BDL135 7723.9 P 0.02 0.22 0.040.00 0.08 BDL135 7723.9 Av 1.87 1.23 2.11 1.62 1.19 BDL135 8782.2 P 0.330.39 0.44 0.10 0.00 0.29 BDL135 8782.2 Av 1.30 1.81 1.43 1.73 1.75 1.20BDL135 8783.1 P 0.00 0.00 0.00 0.00 0.00 0.09 0.00 0.23 BDL135 8783.1 Av2.70 2.40 1.85 3.94 2.48 1.80 1.68 1.20 BDL135 8783.2 P 0.32 0.48 BDL1358783.2 Av 1.74 1.18 BDL135 8785.5 P 0.02 0.07 0.14 0.10 0.01 0.02 0.030.03 0.05 0.20 0.32 BDL135 8785.5 Av 1.16 1.28 1.33 1.67 1.96 1.76 1.662.53 2.26 1.17 1.20 BDL137 7701.2 P 0.25 0.07 0.22 0.09 0.10 BDL1377701.2 Av 1.14 1.48 1.61 1.91 1.68 BDL137 7701.5 P 0.33 0.02 0.21 0.03BDL137 7701.5 Av 2.23 1.36 1.92 1.44 BDL137 7702.1 P 0.30 0.32 0.27 0.050.40 0.08 BDL137 7702.1 Av 1.21 1.44 1.49 1.96 1.41 1.87 BDL137 7703.3 P0.10 0.16 0.12 0.51 0.31 0.03 0.13 0.03 0.08 BDL137 7703.3 Av 1.13 1.151.15 1.12 1.20 1.95 1.17 2.59 1.26 BDL137 7703.7 P 0.38 0.19 0.34 0.150.05 BDL137 7703.7 Av 1.24 2.04 2.78 2.12 1.86 BDL139 8581.5 P 0.38 0.230.30 0.10 0.01 0.15 0.09 0.05 0.02 BDL139 8581.5 Av 1.14 1.16 1.20 1.581.54 1.35 1.37 1.57 1.68 BDL139 8581.5 P 0.57 BDL139 8581.5 Av 1.21BDL139 8581.6 P 0.01 0.01 0.07 0.28 0.19 0.56 0.36 0.12 0.31 0.00 0.00BDL139 8581.6 Av 1.90 2.16 1.46 1.10 1.45 1.17 1.22 2.54 1.35 2.79 4.10BDL139 8581.6 P 0.32 0.43 0.10 0.01 BDL139 8581.6 Av 1.11 1.14 1.64 1.85BDL139 8583.1 P 0.24 0.09 0.12 0.00 0.00 0.03 0.00 0.22 BDL139 8583.1 Av1.13 1.18 1.27 2.25 2.43 1.50 2.22 1.36 BDL139 8583.1 P 0.20 0.59 0.360.27 BDL139 8583.1 Av 1.24 1.12 1.10 1.26 BDL139 8584.1 P 0.31 0.59 0.230.06 0.00 0.09 0.01 0.00 BDL139 8584.1 Av 1.18 1.12 1.30 1.87 1.59 1.371.70 1.77 BDL139 8584.1 P 0.46 0.04 0.02 0.00 0.14 BDL139 8584.1 Av 1.351.66 1.57 1.58 1.32 BDL139 8585.2 P 0.00 0.01 0.02 0.35 0.05 0.05 0.030.05 0.11 BDL139 8585.2 Av 1.61 1.52 1.49 1.27 3.34 2.30 2.18 1.60 2.38BDL139 8585.2 P 0.32 0.23 0.16 0.13 0.17 0.20 0.03 BDL139 8585.2 Av 1.141.22 1.42 1.33 1.29 2.06 1.83 BDL141 8641.3 P 0.02 0.06 0.01 0.09 0.100.16 0.11 0.00 0.00 0.01 BDL141 8641.3 Av 1.82 1.82 1.78 1.51 1.42 2.171.93 2.54 1.95 3.77 BDL141 8641.3 P 0.49 0.10 0.45 0.10 0.49 0.52 0.100.10 BDL141 8641.3 Av 1.10 1.43 1.10 1.69 1.13 1.12 1.51 1.83 BDL1418641.4 P 0.50 0.01 0.14 0.02 0.11 0.17 BDL141 8641.4 Av 1.11 2.36 3.231.98 1.74 1.90 BDL141 8641.4 P 0.10 0.12 0.21 0.05 0.42 0.10 0.08 0.05BDL141 8641.4 Av 1.18 1.66 1.35 1.98 1.13 1.19 1.27 1.64 BDL141 8642.3 P0.01 0.05 0.01 0.13 0.18 0.01 0.03 0.03 0.02 0.00 BDL141 8642.3 Av 1.491.32 1.45 1.33 1.23 1.98 1.68 1.43 1.35 2.64 BDL141 8642.3 P 0.46 0.02BDL141 8642.3 Av 1.26 1.66 BDL141 8642.6 P 0.24 0.15 0.22 0.24 0.15 0.08BDL141 8642.6 Av 1.25 1.45 1.24 1.20 1.19 1.83 BDL141 8642.6 P 0.31BDL141 8642.6 Av 1.30 BDL141 8643.3 P 0.00 0.07 0.08 0.63 0.22 0.06 0.110.01 0.19 0.00 BDL141 8643.3 Av 1.49 1.31 1.33 1.15 1.51 1.47 1.68 1.491.27 3.80 BDL141 8643.3 P 0.24 0.43 0.14 BDL141 8643.3 Av 1.38 1.21 1.38BDL142 8283.2 P BDL142 8283.2 Av BDL142 8284.1 P 0.60 0.18 0.11 0.11BDL142 8284.1 Av 1.10 1.17 1.47 1.36 BDL142 8285.1 P 0.07 0.22 0.06 0.030.08 0.00 0.01 0.19 0.42 BDL142 8285.1 Av 1.51 1.22 1.35 1.36 2.43 2.181.75 2.62 1.22 BDL142 8285.3 P 0.18 0.13 0.65 0.06 0.19 BDL142 8285.3 Av1.22 1.65 1.13 1.89 1.24 BDL142 8285.5 P 0.48 0.38 0.15 0.42 0.33 0.60BDL142 8285.5 Av 1.11 1.11 1.29 1.15 1.22 1.11 BDL143 8411.1 P 0.22 0.310.02 0.02 BDL143 8411.1 Av 1.51 1.22 2.08 1.50 BDL143 8412.2 P 0.28 0.08BDL143 8412.2 Av 1.59 2.30 BDL143 8413.3 P 0.04 0.49 0.14 0.43 0.23BDL143 8413.3 Av 1.49 1.22 1.39 1.31 1.30 BDL143 8414.4 P 0.69 0.17 0.590.01 BDL143 8414.4 Av 1.27 1.42 1.25 1.84 BDL143 8414.5 P 0.17 0.31 0.420.12 BDL143 8414.5 Av 1.28 1.21 1.10 1.40 BDL144 8381.3 P 0.53 0.03 0.11BDL144 8381.3 Av 1.15 1.85 1.39 BDL144 8382.2 P 0.22 0.24 0.40 0.45 0.360.16 0.32 0.08 0.01 0.20 BDL144 8382.2 Av 1.36 1.61 1.34 1.37 1.31 2.951.30 1.73 1.55 1.20 BDL144 8384.4 P 0.04 0.33 0.01 0.01 BDL144 8384.4 Av1.42 2.46 2.51 1.97 BDL144 8385.1 P 0.13 0.06 0.02 0.27 0.03 BDL1448385.1 Av 1.59 1.51 2.26 1.36 1.60 BDL145 8233.2 P 0.08 0.11 0.00 BDL1458233.2 Av 1.39 1.61 1.42 BDL145 8233.3 P 0.57 0.24 0.21 0.04 0.00 BDL1458233.3 Av 1.12 1.71 1.43 2.15 1.51 BDL145 8235.3 P 0.40 0.31 0.01 BDL1458235.3 Av 1.18 1.34 1.55 BDL145 8731.3 P 0.81 0.41 0.47 0.12 0.21 BDL1458731.3 Av 1.12 1.45 1.36 1.60 1.52 BDL145 8734.2 P 0.09 0.32 0.02 0.00BDL145 8734.2 Av 2.95 1.16 2.57 1.38 BDL146 8241.1 P 0.11 0.03 0.07 0.670.06 0.01 0.08 0.00 0.08 0.64 BDL146 8241.1 Av 1.18 1.28 1.20 1.12 1.851.52 1.50 1.52 1.37 1.11 BDL146 8241.3 P 0.06 0.17 0.25 0.01 0.00 BDL1468241.3 Av 1.45 1.23 1.18 4.78 3.57 BDL146 8244.4 P 0.00 0.01 0.04 0.030.53 0.09 0.06 0.02 BDL146 8244.4 Av 1.76 1.74 1.66 1.45 1.45 1.43 1.521.78 BDL146 8244.7 P 0.12 0.31 0.00 0.05 0.01 0.02 BDL146 8244.7 Av 1.571.15 2.44 2.61 2.07 2.32 BDL146 8245.5 P 0.02 0.01 0.01 0.22 0.40 0.000.13 BDL146 8245.5 Av 1.23 1.43 1.32 1.26 1.12 1.33 1.32 BDL42 7771.3 P0.20 0.01 0.29 0.06 0.41 0.04 0.17 0.01 0.17 0.18 0.02 0.04 0.09 BDL427771.3 Av 1.14 1.50 1.15 1.25 1.27 1.67 1.34 1.55 2.06 1.37 1.80 1.301.36 BDL42 7771.5 P 0.43 0.05 0.12 0.00 BDL42 7771.5 Av 1.51 1.15 3.361.69 BDL42 7772.6 P 0.00 0.00 0.01 0.10 BDL42 7772.6 Av 1.85 3.31 1.431.34 BDL42 7774.1 P 0.01 0.01 0.02 BDL42 7774.1 Av 1.57 4.16 2.03 BDL427774.5 P 0.55 0.63 0.02 0.20 0.24 0.06 0.44 BDL42 7774.5 Av 1.11 1.141.56 2.36 1.66 2.21 1.23 BDL51 8021.1 P 0.25 0.01 0.01 BDL51 8021.1 Av1.14 1.92 1.50 BDL51 8022.4 P 0.06 0.02 0.01 BDL51 8022.4 Av 1.15 1.801.59 BDL51 8022.5 P 0.24 0.00 0.06 0.00 BDL51 8022.5 Av 1.11 1.27 2.051.84 BDL51 8022.7 P 0.08 0.00 0.18 0.25 0.00 0.02 0.03 0.34 0.16 BDL518022.7 Av 1.22 1.80 1.18 1.14 3.36 1.71 1.53 1.30 1.34 BDL51 8024.4 P0.02 0.46 0.04 BDL51 8024.4 Av 1.20 1.25 1.38 BDL51 8024.7 P 0.02 0.050.02 BDL51 8024.7 Av 1.34 1.45 1.39 BDL52 7861.1 P 0.05 0.44 0.08 0.100.12 0.02 0.04 0.38 BDL52 7861.1 Av 1.26 1.20 1.73 3.22 1.90 2.82 1.371.19 BDL52 7863.2 P 0.00 0.55 0.52 0.16 0.29 0.08 BDL52 7863.2 Av 1.421.14 1.17 1.31 1.32 1.47 BDL52 7864.2 P 0.10 0.26 0.57 0.22 BDL52 7864.2Av 1.22 3.45 1.10 2.55 BDL52 7864.3 P 0.00 0.27 0.01 0.41 0.46 BDL527864.3 Av 1.28 1.11 1.76 1.16 1.12 BDL52 7864.5 P 0.27 0.47 0.10 BDL527864.5 Av 1.19 1.10 1.12 BDL59 7792.1 P BDL59 7792.1 Av BDL59 7792.2 P0.20 BDL59 7792.2 Av 1.27 BDL59 7792.3 P BDL59 7792.3 Av BDL59 7793.3 P0.01 0.00 0.04 BDL59 7793.3 Av 1.76 1.65 1.45 BDL59 7794.1 P 0.12 BDL597794.1 Av 1.15 BDL65 7824.1 P 0.08 0.11 BDL65 7824.1 Av 1.79 1.37 BDL657825.2 P 0.18 0.48 BDL65 7825.2 Av 1.12 1.16 BDL65 8761.1 P 0.16 0.070.04 0.66 BDL65 8761.1 Av 2.09 1.51 1.39 1.11 BDL65 8762.3 P 0.10 0.150.70 BDL65 8762.3 Av 1.14 1.27 1.15 BDL65 8764.1 P 0.08 0.03 0.39 0.000.39 BDL65 8764.1 Av 1.42 1.81 1.10 1.98 1.46 BDL67 7901.5 P 0.18 0.16BDL67 7901.5 Av 1.71 1.58 BDL67 7902.3 P 0.07 0.10 0.00 BDL67 7902.3 Av1.20 1.22 1.49 BDL67 7902.7 P 0.06 0.03 0.50 0.08 0.35 0.27 BDL67 7902.7Av 1.42 5.05 1.16 4.84 1.19 1.23 BDL67 7903.3 P 0.00 0.19 BDL67 7903.3Av 1.40 1.21 BDL67 7903.5 P 0.01 0.01 0.29 0.02 BDL67 7903.5 Av 1.261.34 1.35 2.25 BDL68 7761.3 P 0.11 0.24 BDL68 7761.3 Av 1.16 1.13 BDL687761.5 P 0.02 0.01 0.68 BDL68 7761.5 Av 1.79 1.42 1.16 BDL68 7761.9 PBDL68 7761.9 Av BDL68 7764.1 P 0.49 BDL68 7764.1 Av 1.16 BDL68 7765.2 P0.11 0.10 0.03 0.26 0.25 0.22 BDL68 7765.2 Av 1.23 1.51 1.84 1.40 1.431.37 BDL78 7911.11 P 0.47 0.27 0.07 0.18 0.01 0.24 0.04 0.01 BDL787911.11 Av 1.10 1.16 1.16 1.19 1.58 1.16 1.24 1.36 BDL78 7911.8 P 0.530.04 0.05 0.22 0.05 0.08 0.09 BDL78 7911.8 Av 1.12 1.31 2.55 1.36 1.871.47 1.63 BDL78 7912.6 P 0.00 0.03 0.44 0.29 0.22 0.04 0.00 0.00 0.53BDL78 7912.6 Av 1.16 1.25 1.13 1.23 2.15 1.85 1.30 1.82 1.16 BDL787913.6 P 0.61 0.27 0.20 0.20 0.47 BDL78 7913.6 Av 1.16 1.83 1.50 1.351.16 BDL78 7913.8 P 0.34 0.43 0.01 0.11 0.02 0.39 BDL78 7913.8 Av 1.141.13 1.57 1.28 1.39 4.27 BDL78 7913.9 P 0.00 0.05 0.08 0.22 BDL78 7913.9Av 2.56 1.75 1.56 1.40 BDL82 7801.1 P 0.05 0.00 BDL82 7801.1 Av 1.971.57 BDL82 7801.2 P 0.11 0.21 0.83 0.33 BDL82 7801.2 Av 1.13 1.14 1.121.33 BDL82 7801.3 P 0.00 0.20 0.01 BDL82 7801.3 Av 1.32 1.78 1.69 BDL827801.3 P 0.09 0.00 0.02 BDL82 7801.3 Av 2.47 5.03 2.49 BDL82 7802.2 P0.00 0.02 0.00 BDL82 7802.2 Av 1.62 3.16 2.56 BDL82 7802.2 P 0.58 BDL827802.2 Av 1.11 BDL82 7802.3 P 0.22 0.07 0.02 BDL82 7802.3 Av 1.33 1.671.46 BDL82 7803.4 P 0.11 0.28 0.03 BDL82 7803.4 Av 1.51 1.28 1.36 BDL827803.8 P 0.11 0.12 0.03 0.41 0.40 BDL82 7803.8 Av 1.55 1.90 2.13 1.371.15 BDL82 7803.9 P 0.00 0.06 0.33 0.35 0.37 0.41 0.01 BDL82 7803.9 Av1.27 1.27 1.15 1.33 1.34 1.16 1.17 BDL82 7803.9 P 0.31 0.12 0.16 0.050.43 BDL82 7803.9 Av 1.12 2.57 1.28 2.36 1.13 BDL82 7808.6 P 0.00 0.020.05 0.04 0.10 0.14 0.17 0.08 0.08 BDL82 7808.6 Av 1.50 1.18 1.22 1.291.42 2.35 1.48 2.32 1.26 BDL89 7812.2 P 0.07 0.00 0.00 0.01 0.00 BDL897812.2 Av 1.16 1.75 1.41 1.50 1.35 BDL89 7812.5 P 0.16 0.04 0.01 BDL897812.5 Av 1.43 3.01 2.30 BDL89 7814.1 P 0.05 0.64 0.07 0.07 BDL89 7814.1Av 1.17 1.13 1.60 1.57 BDL89 7814.4 P 0.19 0.12 0.12 BDL89 7814.4 Av1.40 1.39 1.39 BDL89 7814.5 P 0.11 0.28 0.41 0.02 0.37 0.05 BDL89 7814.5Av 1.30 1.16 2.04 2.01 1.21 1.68 BDL95 7841.2 P 0.03 0.55 0.16 0.70 0.08BDL95 7841.2 Av 1.21 1.23 1.40 1.10 1.37 BDL95 7842.12 P 0.41 0.02 0.100.31 0.17 0.36 0.02 BDL95 7842.12 Av 1.12 1.26 1.26 1.28 1.27 1.17 1.46BDL95 7842.2 P 0.11 0.34 0.00 BDL95 7842.2 Av 1.30 1.19 1.34 BDL957842.8 P 0.18 0.14 0.00 0.33 0.06 BDL95 7842.8 Av 1.22 1.22 1.63 1.191.49 BDL95 7843.4 P 0.02 0.04 0.01 BDL95 7843.4 Av 1.58 2.93 2.20 Table23. “P” = P-value; “Av” = ratio between the averages of event andcontrol. Note that when the average ratio is higher than “1” the effectof exogenous expression of the gene is an increase of the desired trait;“Par” = Parameter according to the parameters listed in Table 22 above;“Ev” = event.

Greenhouse assays—Tables 25, 26 and 27 represent experiments that weredone using greenhouse assays. Table 24 specifies the parameters thatwere measured in the green house assays and which are presented inTables 25, 26 and 27. In cases where a certain event appears more thanonce, the event was tested in several independent experiments.

TABLE 24 Parameter symbol in result Tables 25, 26 and 27 Parameter name1 Rosette Diameter Time point 1 2 Rosette Diameter Time point 2 3Rosette Diameter Time point 3 4 Rosette Diameter Time point 4 5 RosetteArea Time point 1 6 Rosette Area Time point 2 7 Rosette Area Time point3 8 Rosette Area Time point 4 9 Plot Coverage Time point 1 10 PlotCoverage Time point 2 11 Plot Coverage Time point 3 12 Plot CoverageTime point 4 13 Leaf Number Time point 1 14 Leaf Number Time point 2 15Leaf Number Time point 3 16 Leaf Number Time point 4 17 Leaf Blade AreaTime point 1 18 Leaf Blade Area Time point 2 19 Leaf Blade Area Timepoint 3 20 Leaf Blade Area Time point 4 21 Leaf Petiole Area Time point1 22 Leaf Petiole Area Time point 2 23 Leaf Petiole Area Time point 3 24Leaf Petiole Area Time point 4 25 Blade Relative Area Time point 1 26Blade Relative Area Time point 2 27 Blade Relative Area Time point 3 28Blade Relative Area Time point 4 29 Petiole Relative Area Time point 130 Petiole Relative Area Time point 2 31 Petiole Relative Area Timepoint 3 32 Petiole Relative Area Time point 4 33 RGR of Leaf Blade AreaTime point 2 34 RGR of Leaf Blade Area Time point 3 35 RGR of Leaf BladeArea Time point 4 36 RGR of Leaf Number Time point 2 37 RGR of LeafNumber Time point 3 38 RGR of Leaf Number Time point 4 39 RGR of RosetteArea Time point 2 40 RGR of Rosette Area Time point 3 41 RGR of RosetteArea Time point 4 42 RGR of Rosette Diameter Time point 2 43 RGR ofRosette Diameter Time point 3 44 RGR of Rosette Diameter Time point 4 45RGR of Plot Coverage Time point 2 46 RGR of Plot Coverage Time point 347 RGR of Plot Coverage Time point 4 48 Bolting 49 Flowering 50 DryWeight 51 Seed Yield 52 Harvest Index 53 1000 Seeds Weight 54 oilcontent 55 Fresh Weight Table 24.

TABLE 25 Gene Ev. Par. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 BDL957841.5 P BDL95 7841.5 Av. BDL95 7842.12 P BDL95 7842.12 Av. BDL95 7842.2P 0.35 BDL95 7842.2 Av. 1.12 BDL95 7842.8 P BDL95 7842.8 Av. BDL957843.4 P BDL95 7843.4 Av. BDL100 7871.2 P BDL100 7871.2 Av. BDL1007872.2 P BDL100 7872.2 Av. BDL100 7872.3 P 0.35 0.31 0.53 0.33 0.10 0.310.53 0.33 0.10 0.23 0.02 0.23 BDL100 7872.3 Av. 1.11 1.38 1.21 1.19 1.201.38 1.21 1.19 1.20 1.13 1.08 1.38 BDL100 7873.3 P BDL100 7873.3 Av.BDL100 7873.4 P 0.43 0.23 0.57 0.53 0.23 0.57 0.53 0.39 BDL100 7873.4Av. 1.11 1.23 1.12 1.14 1.23 1.12 1.14 1.15 BDL106 7881.1 P 0.24 0.24BDL106 7881.1 Av. 1.13 1.13 BDL106 7881.4 P BDL106 7881.4 Av. BDL1067882.6 P BDL106 7882.6 Av. BDL106 7884.1 P BDL106 7884.1 Av. BDL1067884.9 P BDL106 7884.9 Av. BDL106 7881.1 P BDL106 7881.1 Av. BDL1067881.2 P BDL106 7881.2 Av. BDL106 7882.2 P 0.79 0.79 0.04 BDL106 7882.2Av. 1.13 1.13 1.17 BDL106 7882.4 P BDL106 7882.4 Av. BDL106 7882.5 PBDL106 7882.5 Av. BDL108 8122.2 P BDL108 8122.2 Av. BDL108 8122.3 P 0.020.42 BDL108 8122.3 Av. 1.10 1.11 BDL108 8123.1 P BDL108 8123.1 Av.BDL108 8123.2 P BDL108 8123.2 Av. BDL108 8123.5 P BDL108 8123.5 Av.BDL108 8121.1 P BDL108 8121.1 Av. BDL108 8121.3 P BDL108 8121.3 Av.BDL108 8121.4 P BDL108 8121.4 Av. BDL108 8122.7 P BDL108 8122.7 Av.BDL108 8123.7 P BDL108 8123.7 Av. BDL110 8092.1 P 0.46 0.01 0.56 0.560.46 BDL110 8092.1 Av. 1.13 1.43 1.17 1.17 1.27 BDL110 8092.2 P 0.15BDL110 8092.2 Av. 1.21 BDL110 8092.5 P 0.10 0.01 0.28 0.00 0.30 0.110.53 0.05 0.10 BDL110 8092.5 Av. 1.27 1.52 1.26 1.17 1.11 1.19 1.19 1.111.35 BDL110 8095.2 P 0.01 0.21 0.21 0.05 BDL110 8095.2 Av. 1.44 1.231.23 1.45 BDL111 8102.7 P BDL111 8102.7 Av. BDL111 8103.1 P BDL1118103.1 Av. BDL111 8103.2 P BDL111 8103.2 Av. BDL111 8103.4 P BDL1118103.4 Av. BDL111 8103.5 P 0.04 BDL111 8103.5 Av. 1.04 BDL111 8102.7 PBDL111 8102.7 Av. BDL111 8103.1 P 0.41 0.56 0.39 0.67 0.55 0.67 0.55BDL111 8103.1 Av. 1.11 1.13 1.10 1.12 1.15 1.12 1.15 BDL111 8103.2 PBDL111 8103.2 Av. BDL111 8103.4 P BDL111 8103.4 Av. BDL111 8103.5 PBDL111 8103.5 Av. BDL112 7502.1 P BDL112 7502.1 Av. BDL112 7502.14 PBDL112 7502.14 Av. BDL112 7502.4 P BDL112 7502.4 Av. BDL112 7502.7 PBDL112 7502.7 Av. BDL112 7502.9 P BDL112 7502.9 Av. BDL112 7502.1 P 0.01BDL112 7502.1 Av. 1.36 BDL112 7502.4 P BDL112 7502.4 Av. BDL112 7502.7 PBDL112 7502.7 Av. BDL112 7502.8 P BDL112 7502.8 Av. BDL112 7502.9 PBDL112 7502.9 Av. BDL113 7683.4 P BDL113 7683.4 Av. BDL113 7683.6 PBDL113 7683.6 Av. BDL113 7684.3 P BDL113 7684.3 Av. BDL113 7684.6 PBDL113 7684.6 Av. BDL113 7684.7 P BDL113 7684.7 Av. BDL113 7683.1 PBDL113 7683.1 Av. BDL113 7683.11 P BDL113 7683.11 Av. BDL113 7683.4 PBDL113 7683.4 Av. BDL113 7684.1 P BDL113 7684.1 Av. BDL113 7684.5 PBDL113 7684.5 Av. BDL114 7741.3 P BDL114 7741.3 Av. BDL114 7741.6 P 0.44BDL114 7741.6 Av. 1.10 BDL114 7742.1 P BDL114 7742.1 Av. BDL114 7742.3 PBDL114 7742.3 Av. BDL114 7742.5 P BDL114 7742.5 Av. BDL115 8152.3 P 0.09BDL115 8152.3 Av. 1.13 BDL115 8152.4 P 0.18 0.24 0.06 BDL115 8152.4 Av.1.13 1.12 1.13 BDL115 8154.1 P BDL115 8154.1 Av. BDL115 8155.2 P 0.46BDL115 8155.2 Av. 1.11 BDL115 8155.4 P BDL115 8155.4 Av. BDL115 8152.3 PBDL115 8152.3 Av. BDL115 8152.4 P BDL115 8152.4 Av. BDL115 8154.1 PBDL115 8154.1 Av. BDL115 8155.2 P BDL115 8155.2 Av. BDL115 8155.4 P 0.06BDL115 8155.4 Av. 1.15 BDL116 7481.2 P BDL116 7481.2 Av. BDL116 7481.7 PBDL116 7481.7 Av. BDL116 7481.8 P BDL116 7481.8 Av. BDL116 7482.2 PBDL116 7482.2 Av. BDL116 7485.1 P BDL116 7485.1 Av. BDL119 7732.2 P 0.41BDL119 7732.2 Av. 1.16 BDL119 7733.2 P 0.18 BDL119 7733.2 Av. 1.20BDL119 7734.1 P BDL119 7734.1 Av. BDL119 7734.5 P 0.08 BDL119 7734.5 Av.1.26 BDL119 7734.7 P 0.07 0.00 0.08 0.64 0.39 0.64 0.39 0.53 BDL1197734.7 Av. 1.33 1.57 1.09 1.13 1.19 1.13 1.19 1.27 BDL120 7891.3 PBDL120 7891.3 Av. BDL120 7892.4 P BDL120 7892.4 Av. BDL120 7892.6 PBDL120 7892.6 Av. BDL120 7893.2 P BDL120 7893.2 Av. BDL120 7893.5 P 0.140.52 0.12 0.34 0.43 0.52 0.12 0.34 0.43 0.26 0.00 0.35 BDL120 7893.5 Av.1.18 1.13 1.28 1.19 1.17 1.13 1.28 1.19 1.17 1.14 1.10 1.13 BDL1227513.1 P BDL122 7513.1 Av. BDL122 7513.1 P 0.07 BDL122 7513.1 Av. 1.09BDL122 7513.14 P BDL122 7513.14 Av. BDL122 7513.9 P BDL122 7513.9 Av.BDL122 7514.3 P 0.25 0.51 0.47 0.11 0.27 0.28 0.20 0.07 0.07 0.01 0.33BDL122 7514.3 Av. 1.29 1.18 1.14 1.35 1.36 1.34 1.17 1.17 1.15 1.25 1.21BDL122 7513.1 P 0.02 0.06 0.08 0.03 0.07 0.23 0.23 0.03 0.07 0.23 0.230.02 BDL122 7513.1 Av. 1.18 1.13 1.13 1.25 1.20 1.12 1.12 1.25 1.20 1.121.12 1.17 BDL122 7513.14 P BDL122 7513.14 Av. BDL122 7513.9 P 0.28 0.190.19 BDL122 7513.9 Av. 1.14 1.12 1.12 BDL122 7514.3 P BDL122 7514.3 Av.BDL123 8082.1 P 0.21 0.32 0.20 0.25 0.32 0.20 0.25 0.26 BDL123 8082.1Av. 1.15 1.21 1.17 1.12 1.21 1.17 1.12 1.20 BDL123 8082.3 P 0.00 0.000.02 0.05 0.00 0.02 0.05 0.01 0.00 0.00 BDL123 8082.3 Av. 1.23 1.32 1.251.23 1.32 1.25 1.23 1.26 1.16 1.18 BDL123 8082.6 P 0.16 0.24 0.24 0.20BDL123 8082.6 Av. 1.13 1.11 1.11 1.12 BDL123 8083.2 P BDL123 8083.2 Av.BDL123 8083.3 P 0.00 0.00 0.02 0.00 0.00 0.00 0.00 0.00 0.00 0.03 0.290.01 0.00 BDL123 8083.3 Av. 1.33 1.19 1.18 1.64 1.46 1.44 1.64 1.46 1.441.30 1.10 1.12 1.46 BDL124 8482.1 P BDL124 8482.1 Av. BDL125 7491.1 P0.00 0.08 0.34 0.18 0.08 0.34 0.18 0.00 BDL125 7491.1 Av. 1.24 1.29 1.231.20 1.29 1.23 1.20 1.28 BDL125 7491.5 P 0.58 0.58 0.23 BDL125 7491.5Av. 1.13 1.13 1.12 BDL125 7492.5 P BDL125 7492.5 Av. BDL125 7494.1 P0.00 BDL125 7494.1 Av. 1.12 BDL125 7495.5 P BDL125 7495.5 Av. BDL1287711.3 P 0.26 BDL128 7711.3 Av. 1.14 BDL128 8361.5 P 0.07 0.28 0.28 0.43BDL128 8361.5 Av. 1.10 1.14 1.14 1.12 BDL128 8362.2 P 0.00 0.05 0.160.02 0.23 0.25 0.11 0.06 0.01 BDL128 8362.2 Av. 1.21 1.10 1.12 1.24 1.141.16 1.18 1.08 1.15 BDL128 8363.2 P BDL128 8363.2 Av. BDL128 8365.2 PBDL128 8365.2 Av. BDL129 7691.4 P BDL129 7691.4 Av. BDL129 7691.6 PBDL129 7691.6 Av. BDL129 7692.2 P 0.14 0.00 0.41 BDL129 7692.2 Av. 1.111.12 1.15 BDL129 7692.6 P BDL129 7692.6 Av. BDL129 7693.1 P BDL1297693.1 Av. BDL130 7663.1 P 0.03 0.53 BDL130 7663.1 Av. 1.33 1.15 BDL1307663.3 P BDL130 7663.3 Av. BDL130 7663.6 P 0.00 0.73 0.26 BDL130 7663.6Av. 1.49 1.12 1.37 BDL130 7664.5 P 0.31 0.02 0.52 0.66 0.38 BDL1307664.5 Av. 1.17 1.51 1.28 1.22 1.40 BDL131 7461.2 P 0.00 0.20 0.00 0.130.01 0.05 0.00 0.13 0.01 0.05 0.08 BDL131 7461.2 Av. 1.21 1.13 1.41 1.131.34 1.26 1.41 1.13 1.34 1.26 1.53 BDL131 7461.4 P 0.01 0.01 0.05 0.050.17 0.22 0.24 0.41 0.58 0.47 0.01 0.02 0.06 0.02 BDL131 7461.4 Av. 1.271.42 1.18 1.19 1.18 1.33 1.11 1.12 1.11 1.12 1.12 1.09 1.08 1.38 BDL1317462.2 P 0.00 0.04 0.08 0.05 0.35 0.03 0.11 0.00 0.35 0.03 0.11 0.000.36 0.25 0.33 BDL131 7462.2 Av. 1.37 1.21 1.15 1.11 1.43 1.50 1.39 1.311.43 1.50 1.39 1.31 1.14 1.17 1.40 BDL131 7463.4 P 0.01 0.13 0.15 0.130.15 0.06 0.21 BDL131 7463.4 Av. 1.13 1.13 1.15 1.13 1.15 1.04 1.12BDL131 7464.5 P 0.05 BDL131 7464.5 Av. 1.06 BDL132 7471.1 P 0.00 0.280.06 0.35 0.30 0.11 0.22 0.30 0.11 0.22 0.43 0.09 0.02 BDL132 7471.1 Av.1.27 1.22 1.20 1.12 1.50 1.33 1.23 1.50 1.33 1.23 1.23 1.12 1.14 BDL1327471.1 P 0.07 0.31 0.14 0.56 0.28 0.09 0.07 0.56 0.28 0.09 0.07 0.190.08 BDL132 7471.4 Av. 1.08 1.11 1.12 1.15 1.27 1.21 1.19 1.15 1.27 1.211.19 1.14 1.18 BDL132 7472.4 P BDL132 7472.4 Av. BDL132 7473.1 P 0.020.06 0.03 0.13 0.03 0.13 BDL132 7473.1 Av. 1.12 1.09 1.31 1.15 1.31 1.15BDL132 7474.4 P BDL132 7474.4 Av. BDL132 7471.1 P BDL132 7471.1 Av.BDL132 7471.4 P BDL132 7471.4 Av. BDL132 7472.4 P BDL132 7472.4 Av.BDL132 7473.1 P BDL132 7473.1 Av. BDL132 7475.4 P BDL132 7475.4 Av.BDL133 8161.1 P 0.27 BDL133 8161.1 Av. 1.34 BDL133 8161.2 P 0.07 0.000.03 0.01 0.28 0.05 0.02 0.46 BDL133 8161.2 Av. 1.29 1.52 1.48 1.16 1.131.39 1.58 1.12 BDL133 8161.3 P 0.06 0.32 0.32 BDL133 8161.3 Av. 1.471.13 1.13 BDL133 8161.4 P 0.15 BDL133 8161.4 Av. 1.32 BDL133 8162.1 P0.07 BDL133 8162.1 Av. 1.26 BDL133 8162.3 P 0.03 0.31 BDL133 8162.3 Av.1.34 1.21 BDL133 8162.5 P 0.30 0.00 0.24 0.02 0.01 0.24 0.02 0.01 0.17BDL133 8162.5 Av. 1.15 1.53 1.27 1.17 1.22 1.27 1.17 1.22 1.45 BDL1338163.2 P 0.27 0.59 BDL133 8163.2 Av. 1.33 1.29 BDL134 7671.2 P BDL1347671.2 Av. BDL134 7672.1 P BDL134 7672.1 Av. BDL134 7673.1 P BDL1347673.1 Av. BDL134 7673.2 P 0.21 BDL134 7673.2 Av. 1.19 BDL135 7722.1 P0.03 0.55 BDL135 7722.1 Av. 1.35 1.12 BDL135 7723.1 P 0.01 BDL135 7723.1Av. 1.40 BDL135 7723.3 P 0.27 BDL135 7723.3 Av. 1.17 BDL135 7723.8 P0.11 BDL135 7723.8 Av. 1.31 BDL135 7723.9 P 0.35 0.00 0.36 0.37 0.030.01 0.36 0.37 0.03 0.01 0.24 BDL135 7723.9 Av. 1.18 1.57 1.41 1.17 1.151.17 1.41 1.17 1.15 1.17 1.54 BDL136 7751.4 P 0.36 0.01 0.55 BDL1367751.4 Av. 1.17 1.39 1.12 BDL136 7751.5 P 0.25 BDL136 7751.5 Av. 1.16BDL136 7751.8 P 0.06 0.01 0.33 0.18 0.06 0.17 0.20 0.18 0.06 0.17 0.200.24 0.36 0.06 BDL136 7751.8 Av. 1.31 1.65 1.16 1.25 1.33 1.28 1.33 1.251.33 1.28 1.33 1.24 1.13 1.11 BDL136 7752.6 P 0.01 0.10 BDL136 7752.6Av. 1.44 1.36 BDL137 7701.2 P 0.00 0.30 0.29 0.30 0.29 BDL137 7701.2 Av.1.51 1.16 1.13 1.16 1.13 BDL137 7701.5 P 0.09 BDL137 7701.5 Av. 1.34BDL137 7701.6 P 0.06 0.67 BDL137 7701.6 Av. 1.44 1.21 BDL137 7702.1 PBDL137 7702.1 Av. BDL137 7703.2 P 0.06 BDL137 7703.2 Av. 1.38 BDL1377703.3 P 0.16 BDL137 7703.3 Av. 1.20 BDL137 7703.7 P 0.13 0.46 BDL1377703.7 Av. 1.36 1.27 BDL139 8131.1 P BDL139 8131.1 Av. BDL139 8131.2 PBDL139 8131.2 Av. BDL139 8132.7 P BDL139 8132.7 Av. BDL139 8133.2 PBDL139 8133.2 Av. BDL141 8141.2 P 0.06 0.30 BDL141 8141.2 Av. 1.39 1.21BDL141 8142.2 P BDL141 8142.2 Av. BDL142 8282.1 P 0.18 BDL142 8282.1 Av.1.34 BDL142 8283.1 P 0.11 BDL142 8283.1 Av. 1.24 BDL142 8283.2 P 0.010.47 0.47 0.06 BDL142 8283.2 Av. 1.45 1.18 1.18 1.42 BDL142 8284.1 P0.02 BDL142 8284.1 Av. 1.36 BDL142 8285.3 P 0.13 BDL142 8285.3 Av. 1.23BDL142 8285.5 P 0.49 0.06 0.51 0.57 0.51 0.57 0.24 BDL142 8285.5 Av.1.10 1.53 1.12 1.11 1.12 1.11 1.24 BDL143 8411.1 P 0.33 0.00 0.28 0.030.00 0.06 0.28 0.03 0.00 0.06 0.30 0.08 0.03 BDL143 8411.1 Av. 1.18 1.731.47 1.36 1.28 1.32 1.47 1.36 1.28 1.32 1.51 1.12 1.17 BDL143 8411.5 P0.37 0.04 0.64 BDL143 8411.5 Av. 1.13 1.37 1.12 BDL143 8412.2 P 0.24BDL143 8412.2 Av. 1.20 BDL143 8412.4 P BDL143 8412.4 Av. BDL143 8413.3 P0.21 BDL143 8413.3 Av. 1.18 BDL143 8414.4 P 0.35 BDL143 8414.4 Av. 1.35BDL143 8414.5 P 0.19 BDL143 8414.5 Av. 1.19 BDL144 8384.1 P 0.05 BDL1448384.1 Av. 1.30 BDL144 8384.5 P 0.06 BDL144 8384.5 Av. 1.29 BDL1448385.1 P BDL144 8385.1 Av. BDL145 8233.2 P 0.10 BDL145 8233.2 Av. 1.26BDL145 8233.3 P BDL145 8233.3 Av. BDL145 8235.1 P 0.13 BDL145 8235.1 Av.1.24 BDL145 8235.3 P BDL145 8235.3 Av. BDL145 8235.4 P BDL145 8235.4 Av.BDL146 8241.1 P 0.42 0.13 0.32 0.60 0.32 0.60 0.15 BDL146 8241.1 Av.1.12 1.46 1.18 1.13 1.18 1.13 1.30 BDL146 8241.3 P 0.08 BDL146 8241.3Av. 1.28 BDL146 8243.2 P 0.06 0.55 BDL146 8243.2 Av. 1.37 1.12 BDL1468243.5 P 0.45 BDL146 8243.5 Av. 1.21 BDL146 8244.4 P 0.07 0.33 0.27 0.420.33 0.27 0.42 0.21 BDL146 8244.4 Av. 1.57 1.16 1.26 1.22 1.16 1.26 1.221.11 BDL146 8244.7 P 0.47 BDL146 8244.7 Av. 1.10 BDL146 8245.2 P 0.02BDL146 8245.2 Av. 1.35 BDL146 8245.5 P 0.32 0.00 0.23 0.21 0.47 0.230.21 0.47 0.05 BDL146 8245.5 Av. 1.16 1.51 1.21 1.13 1.11 1.21 1.13 1.111.45 BDL42 7771.1 P BDL42 7771.1 Av. BDL42 7772.1 P BDL42 7772.1 Av.BDL42 7772.7 P BDL42 7772.7 Av. BDL42 7774.1 P BDL42 7774.1 Av. BDL427774.2 P BDL42 7774.2 Av. BDL42 7774.4 P 0.56 BDL42 7774.4 Av. 1.11BDL46 7833.3 P BDL46 7833.3 Av. BDL46 7833.4 P 0.27 0.10 BDL46 7833.4Av. 1.10 1.08 BDL46 7833.5 P BDL46 7833.5 Av. BDL46 7833.6 P BDL467833.6 Av. BDL46 7834.1 P BDL46 7834.1 Av. BDL46 7833.1 P BDL46 7833.1Av. BDL46 7833.3 P BDL46 7833.3 Av. BDL46 7833.4 P BDL46 7833.4 Av.BDL46 7833.5 P BDL46 7833.5 Av. BDL46 7834.4 P 0.19 BDL46 7834.4 Av.1.25 BDL51 7291.1 P BDL51 7291.1 Av. BDL51 8021.1 P BDL51 8021.1 Av.BDL51 8022.4 P BDL51 8022.4 Av. BDL51 8022.5 P BDL51 8022.5 Av. BDL518024.4 P BDL51 8024.4 Av. BDL51 8024.7 P BDL51 8024.7 Av. BDL52 7861.1 P0.02 BDL52 7861.1 Av. 1.08 BDL52 7861.5 P BDL52 7861.5 Av. BDL52 7863.2P 0.17 BDL52 7863.2 Av. 1.12 BDL52 7864.5 P 0.14 0.14 0.14 0.14 BDL527864.5 Av. 1.16 1.12 1.16 1.12 BDL54 7781.1 P BDL54 7781.1 Av. BDL547781.4 P BDL54 7781.4 Av. BDL54 7784.3 P BDL54 7784.3 Av. BDL54 7784.5 PBDL54 7784.5 Av. BDL54 7785.4 P BDL54 7785.4 Av. BDL54 7781.1 P BDL547781.1 Av. BDL54 7781.4 P BDL54 7781.4 Av. BDL54 7784.3 P BDL54 7784.3Av. BDL54 7785.4 P BDL54 7785.4 Av. BDL54 7785.8 P BDL54 7785.8 Av.BDL56 7181.2 P BDL56 7181.2 Av. BDL56 8301.1 P 0.00 0.05 0.04 0.04 0.310.00 0.02 0.02 0.31 0.00 0.02 0.02 0.38 0.07 0.02 0.28 BDL56 8301.1 Av.1.26 1.18 1.21 1.12 1.36 1.33 1.33 1.33 1.36 1.33 1.33 1.33 1.16 1.091.09 1.32 BDL56 8301.3 P BDL56 8301.3 Av. BDL56 8304.1 P 0.14 0.04 0.450.09 0.06 0.45 0.09 0.06 0.58 0.06 0.17 0.39 BDL56 8304.1 Av. 1.19 1.111.28 1.17 1.16 1.28 1.17 1.16 1.14 1.09 1.12 1.23 BDL56 8305.1 P BDL568305.1 Av. BDL56 8301.1 P BDL56 8301.1 Av. BDL56 8301.2 P 0.39 BDL568301.2 Av. 1.24 BDL56 8301.3 P BDL56 8301.3 Av. BDL56 8303.1 P 0.43BDL56 8303.1 Av. 1.11 BDL56 8303.2 P BDL56 8303.2 Av. BDL59 7792.1 P0.00 0.05 0.14 0.23 0.06 0.04 0.23 0.06 0.04 0.08 0.00 0.06 0.09 BDL597792.1 Av. 1.21 1.15 1.10 1.25 1.24 1.24 1.25 1.24 1.24 1.16 1.17 1.131.16 BDL59 7792.2 P 0.05 0.20 BDL59 7792.2 Av. 1.09 1.12 BDL59 7792.3 P0.21 0.16 0.03 0.05 0.57 0.03 0.12 0.10 0.57 0.03 0.12 0.10 0.58 0.070.09 0.00 0.62 BDL59 7792.3 Av. 1.12 1.13 1.17 1.11 1.22 1.23 1.18 1.131.22 1.23 1.18 1.13 1.14 1.09 1.12 1.20 1.13 BDL59 7793.3 P 0.07 0.09BDL59 7793.3 Av. 1.05 1.07 BDL59 7794.1 P BDL59 7794.1 Av. BDL60 8011.4P 0.47 0.40 0.42 0.47 0.40 0.42 0.27 0.00 BDL60 8011.4 Av. 1.18 1.211.19 1.18 1.21 1.19 1.11 1.11 BDL60 8011.7 P BDL60 8011.7 Av. BDL608013.4 P BDL60 8013.4 Av. BDL60 8013.6 P BDL60 8013.6 Av. BDL60 8014.5 PBDL60 8014.5 Av. BDL60 8013.6 P BDL60 8013.6 Av. BDL60 8014.2 P 0.00BDL60 8014.2 Av. 1.20 BDL60 8014.7 P 0.48 BDL60 8014.7 Av. 1.27 BDL608014.8 P BDL60 8014.8 Av. BDL65 7824.1 P BDL65 7824.1 Av. BDL65 7825.2 P0.36 BDL65 7825.2 Av. 1.27 BDL65 8473.2 P BDL65 8473.2 Av. BDL65 8474.1P 0.03 0.50 0.50 0.30 BDL65 8474.1 Av. 1.32 1.14 1.14 1.36 BDL67 7901.5P BDL67 7901.5 Av. BDL67 7902.3 P BDL67 7902.3 Av. BDL67 7902.7 P BDL677902.7 Av. BDL67 7903.3 P BDL67 7903.3 Av. BDL67 7903.5 P BDL67 7903.5Av. BDL68 7761.3 P BDL68 7761.3 Av. BDL68 7761.8 P BDL68 7761.8 Av.BDL68 7761.9 P BDL68 7761.9 Av. BDL68 7763.2 P BDL68 7763.2 Av. BDL687764.1 P BDL68 7764.1 Av. BDL78 7911.11 P 0.03 0.55 BDL78 7911.11 Av.1.34 1.12 BDL78 7911.8 P 0.31 BDL78 7911.8 Av. 1.20 BDL78 7911.9 P BDL787911.9 Av. BDL78 7912.6 P 0.11 BDL78 7912.6 Av. 1.23 BDL78 7913.11 P0.48 BDL78 7913.11 Av. 1.11 BDL78 7913.3 P 0.00 0.47 0.47 0.19 BDL787913.3 Av. 1.46 1.13 1.13 1.33 BDL78 7913.6 P BDL78 7913.6 Av. BDL787913.8 P 0.39 BDL78 7913.8 Av. 1.12 BDL78 7913.9 P 0.01 BDL78 7913.9 Av.1.38 BDL82 7801.1 P BDL82 7801.1 Av. BDL82 7801.3 P BDL82 7801.3 Av.BDL82 7802.2 P BDL82 7802.2 Av. BDL82 7802.3 P BDL82 7802.3 Av. BDL827803.9 P 0.14 0.02 0.01 0.02 0.00 0.13 0.01 0.08 0.00 0.13 0.01 0.080.01 0.02 0.00 0.12 0.00 BDL82 7803.9 Av. 1.29 1.13 1.16 1.14 1.48 1.251.38 1.30 1.48 1.25 1.38 1.30 1.26 1.14 1.11 1.12 1.34 BDL89 7812.2 P0.07 BDL89 7812.2 Av. 1.05 BDL89 7812.5 P BDL89 7812.5 Av. BDL89 7814.1P 0.19 BDL89 7814.1 Av. 1.20 BDL89 7814.4 P BDL89 7814.4 Av. BDL897814.5 P BDL89 7814.5 Av. Table 25. “P” = P-value; “Av” = ratio betweenthe averages of event and control. Note that when the average ratio ishigher than “1” the effect of exogenous expression of the gene is anincrease of the desired trait; “Par” = Parameter according to theparameters listed in Table 24 above; “Ev” = event.

TABLE 26 Gene Ev. Par. 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 3334 BDL95 7841.5 P 0.71 0.78 0.70 0.66 BDL95 7841.5 Av. 1.23 1.18 1.191.31 BDL95 7842.12 P 0.67 0.75 0.19 0.70 0.32 BDL95 7842.12 Av. 1.111.18 2.66 1.28 2.84 BDL95 7842.2 P 0.78 0.47 0.41 BDL95 7842.2 Av. 1.1155.83 28.99 BDL95 7842.8 P 0.56 0.56 0.46 0.35 0.41 0.46 BDL95 7842.8Av. 1.36 1.29 3.76 1.56 1.57 4.32 BDL95 7843.4 P 0.03 0.56 0.54 0.000.53 0.16 0.48 0.54 BDL95 7843.4 Av. 2.00 2.47 1.84 1.84 2.94 1.23 2.182.06 BDL100 7871.2 P 0.72 0.47 BDL100 7871.2 Av. 1.17 1.28 BDL100 7872.2P 0.00 0.82 0.00 0.13 0.75 0.57 0.36 BDL100 7872.2 Av. 1.63 1.13 1.531.18 1.18 1.35 1.11 BDL100 7872.3 P 0.47 0.39 0.76 0.24 0.76 BDL1007872.3 Av. 1.26 1.11 2.13 1.10 2.04 BDL100 7873.3 P 0.24 0.19 0.22 0.150.59 BDL100 7873.3 Av. 1.13 6.24 1.17 5.93 1.16 BDL100 7873.4 P 0.330.54 0.55 0.55 0.57 0.31 BDL100 7873.4 Av. 1.17 1.87 2.80 2.06 2.78 2.02BDL106 7881.1 P 0.03 0.57 0.03 0.60 0.00 BDL106 7881.1 Av. 1.21 1.331.21 1.34 1.32 BDL106 7881.4 P 0.44 0.63 0.35 0.27 0.27 0.21 0.01 0.34BDL106 7881.4 Av. 5.50 1.25 1.74 2.42 1.63 1.81 3.39 1.20 BDL106 7882.6P 0.48 0.31 0.61 0.70 0.27 0.06 BDL106 7882.6 Av. 1.37 1.61 1.35 1.141.98 1.49 BDL106 7884.1 P 0.22 0.46 0.65 0.31 0.47 0.00 0.60 BDL1067884.1 Av. 1.87 2.65 1.11 1.67 3.05 1.34 1.56 BDL106 7884.9 P 0.50 0.410.67 0.22 0.48 0.38 0.57 0.05 0.44 BDL106 7884.9 Av. 2.57 1.15 1.20 1.761.86 1.47 1.56 2.59 1.13 BDL106 7881.1 P 0.59 0.66 0.47 0.58 0.61 0.230.38 BDL106 7881.1 Av. 4.09 1.25 1.56 4.38 1.29 1.32 1.69 BDL106 7881.2P 0.22 0.01 0.30 0.17 0.22 0.58 BDL106 7881.2 Av. 1.16 5.82 1.38 6.351.42 2.85 BDL106 7882.2 P 0.10 0.57 0.46 0.59 0.03 0.65 0.54 0.66 0.15BDL106 7882.2 Av. 1.32 1.75 1.84 1.58 1.05 1.55 1.82 1.64 1.68 BDL1067882.4 P 0.77 0.60 0.67 0.66 0.46 BDL106 7882.4 Av. 1.23 1.49 1.13 1.421.71 BDL106 7882.5 P 0.21 0.75 0.21 0.84 BDL106 7882.5 Av. 1.53 1.191.58 1.19 BDL108 8122.2 P 0.08 0.39 0.17 0.30 0.48 0.45 BDL108 8122.2Av. 2.85 1.94 2.11 3.00 1.38 2.73 BDL108 8122.3 P 0.46 0.00 0.50 0.080.04 0.09 BDL108 8122.3 Av. 2.61 1.46 1.58 1.20 1.50 1.18 BDL108 8123.1P 0.20 0.00 0.22 0.41 BDL108 8123.1 Av. 1.96 1.91 1.21 1.59 BDL1088123.2 P 0.30 0.42 0.20 0.38 0.62 0.53 BDL108 8123.2 Av. 3.17 3.64 2.364.07 1.26 2.81 BDL108 8123.5 P 0.42 0.31 0.27 0.04 0.36 0.35 0.31 0.14BDL108 8123.5 Av. 2.96 1.12 1.42 3.68 1.86 1.38 1.68 4.42 BDL108 8121.1P 0.12 0.63 0.26 0.73 0.10 0.51 0.00 0.18 0.64 BDL108 8121.1 Av. 4.751.33 1.54 1.19 6.16 1.59 2.33 2.15 1.28 BDL108 8121.3 P 0.65 0.35 0.440.68 0.62 0.35 0.50 BDL108 8121.3 Av. 2.11 1.24 1.36 1.88 1.12 1.61 1.34BDL108 8121.4 P 0.44 0.48 0.44 0.13 0.14 0.74 BDL108 8121.4 Av. 2.831.17 3.66 1.46 1.32 1.14 BDL108 8122.7 P 0.67 0.28 0.63 0.84 0.30 0.050.85 BDL108 8122.7 Av. 4.00 1.52 5.66 1.22 1.64 2.58 1.56 BDL108 8123.7P 0.71 0.38 0.06 0.32 0.72 0.23 0.00 0.08 BDL108 8123.7 Av. 2.15 1.541.18 1.32 2.11 1.77 1.48 1.67 BDL110 8092.1 P 0.00 BDL110 8092.1 Av.1.28 BDL110 8092.2 P 0.52 0.24 0.44 0.22 0.37 0.26 0.45 0.27 BDL1108092.2 Av. 1.35 1.76 1.62 1.14 1.48 1.96 1.92 1.22 BDL110 8092.5 P 0.230.01 0.01 0.37 0.09 BDL110 8092.5 Av. 1.16 1.15 1.17 1.28 1.32 BDL1108095.2 P 0.00 BDL110 8095.2 Av. 1.24 BDL111 8102.7 P 0.00 0.25 0.01 0.020.30 0.25 BDL111 8102.7 Av. 1.62 1.11 1.57 1.42 1.12 1.26 BDL111 8103.1P 0.41 0.06 0.08 0.32 0.36 0.29 0.30 0.12 0.32 BDL111 8103.1 Av. 2.151.27 1.11 2.44 1.85 1.76 1.40 3.38 1.37 BDL111 8103.2 P 0.00 0.00 0.13BDL111 8103.2 Av. 1.79 1.66 1.20 BDL111 8103.4 P 0.41 0.12 0.00 0.500.28 0.22 0.00 0.40 BDL111 8103.4 Av. 1.87 1.44 1.52 2.06 1.58 1.72 1.872.83 BDL111 8103.5 P 0.06 0.71 0.34 0.00 0.12 0.35 0.18 0.00 0.73 BDL1118103.5 Av. 1.77 1.11 1.21 4.29 1.66 1.25 1.37 5.17 1.14 BDL111 8102.7 P0.46 0.20 0.63 0.13 0.44 0.28 0.34 0.06 BDL111 8102.7 Av. 2.95 1.35 1.151.51 3.43 1.57 1.37 1.68 BDL111 8103.1 P 0.57 0.65 0.26 0.27 0.24 0.32BDL111 8103.1 Av. 1.12 1.11 1.13 2.19 2.28 1.58 BDL111 8103.2 P 0.960.51 0.20 0.88 0.68 0.34 0.33 BDL111 8103.2 Av. 1.35 1.16 1.53 2.21 1.211.47 2.07 BDL111 8103.4 P 0.90 0.79 0.83 BDL111 8103.4 Av. 1.51 2.471.42 BDL111 8103.5 P 0.88 0.58 0.82 0.53 0.69 BDL111 8103.5 Av. 2.941.19 4.21 1.46 1.37 BDL112 7502.1 P 0.69 0.43 0.44 0.37 0.32 BDL1127502.1 Av. 1.20 2.10 1.46 2.26 1.63 BDL112 7502.14 P 0.52 0.50 0.57 0.500.48 0.47 0.46 BDL112 7502.14 Av. 1.67 3.18 1.34 1.44 3.42 1.55 2.15BDL112 7502.4 P 0.19 0.75 0.11 0.29 0.02 0.44 0.65 0.51 BDL112 7502.4Av. 2.17 1.18 2.04 1.46 1.24 1.48 1.24 1.36 BDL112 7502.7 P 0.19 0.190.07 0.18 0.00 0.84 0.25 BDL112 7502.7 Av. 1.88 1.36 1.70 1.36 1.44 1.131.52 BDL112 7502.9 P 0.30 0.62 0.62 0.26 0.16 0.50 BDL112 7502.9 Av.2.12 1.23 1.22 1.20 2.29 1.30 BDL112 7502.1 P 0.42 0.41 0.41 0.61 BDL1127502.1 Av. 1.29 1.15 1.27 1.40 BDL112 7502.4 P 0.58 0.55 0.48 0.18BDL112 7502.4 Av. 1.19 1.84 2.27 2.78 BDL112 7502.7 P 0.64 0.82 0.610.71 0.72 BDL112 7502.7 Av. 3.88 1.14 4.86 1.19 1.36 BDL112 7502.8 P0.54 0.67 0.57 0.51 0.54 0.60 BDL112 7502.8 Av. 2.50 1.20 1.40 2.33 1.411.52 BDL112 7502.9 P 0.07 0.79 0.07 0.48 0.62 0.56 BDL112 7502.9 Av.4.27 1.11 4.93 1.12 1.23 1.22 BDL113 7683.4 P 0.13 0.00 0.69 0.18 0.350.10 0.58 0.24 BDL113 7683.4 Av. 1.51 1.24 1.63 1.76 1.13 1.53 2.03 1.60BDL113 7683.6 P 0.06 0.10 0.09 0.20 0.01 0.04 0.00 0.02 0.00 BDL1137683.6 Av. 2.09 1.38 1.25 1.80 1.93 2.10 1.67 2.80 1.59 BDL113 7684.3 P0.24 0.56 0.38 0.62 0.05 0.53 0.35 0.52 BDL113 7684.3 Av. 1.45 1.34 1.501.69 1.42 1.61 1.90 2.95 BDL113 7684.6 P 0.12 0.04 0.54 0.17 0.00 0.51BDL113 7684.6 Av. 1.81 1.29 3.37 1.68 1.63 4.60 BDL113 7684.7 P 0.570.90 0.31 0.70 BDL113 7684.7 Av. 1.15 1.19 1.59 1.88 BDL113 7683.1 P0.18 0.41 0.63 0.16 0.23 0.33 0.06 0.11 0.61 BDL113 7683.1 Av. 4.92 1.371.11 1.68 6.15 1.83 1.32 2.01 2.09 BDL113 7683.11 P 0.00 0.00 BDL1137683.11 Av. 3.76 4.28 BDL113 7683.4 P 0.29 0.47 0.94 0.36 0.47 0.78BDL113 7683.4 Av. 1.28 1.32 1.72 1.56 1.62 1.47 BDL113 7684.1 P BDL1137684.1 Av. BDL113 7684.5 P 0.00 0.00 BDL113 7684.5 Av. 3.01 4.03 BDL1147741.3 P 0.41 0.12 0.30 0.40 0.00 0.23 0.53 BDL114 7741.3 Av. 3.87 1.923.64 4.33 2.51 4.94 2.47 BDL114 7741.6 P 0.36 0.50 0.01 0.58 0.49 0.060.43 BDL114 7741.6 Av. 1.37 12.18 2.96 1.16 4.68 2.99 3.15 BDL114 7742.1P 0.36 0.30 0.30 0.50 BDL114 7742.1 Av. 1.56 1.35 1.21 1.29 BDL1147742.3 P 0.39 0.23 0.59 0.29 0.00 0.02 0.10 BDL114 7742.3 Av. 4.22 1.731.24 2.30 2.68 1.73 1.70 BDL114 7742.5 P 0.16 0.25 0.18 0.63 0.00 0.250.13 0.27 0.53 BDL114 7742.5 Av. 3.66 1.85 1.38 1.33 2.39 2.81 2.01 2.231.62 BDL115 8152.3 P 0.05 0.34 BDL115 8152.3 Av. 1.28 1.14 BDL115 8152.4P 0.13 0.71 0.08 0.79 0.08 0.39 BDL115 8152.4 Av. 1.16 1.15 2.16 1.112.20 1.39 BDL115 8154.1 P 0.40 0.36 0.60 BDL115 8154.1 Av. 2.40 2.881.11 BDL115 8155.2 P 0.51 0.26 0.52 0.26 0.54 BDL115 8155.2 Av. 2.702.22 3.09 2.28 2.66 BDL115 8155.4 P 0.36 0.48 0.25 0.47 0.53 BDL1158155.4 Av. 2.31 1.84 1.88 2.66 2.33 BDL115 8152.3 P 0.43 0.35 0.01 0.360.37 0.20 0.24 0.09 BDL115 8152.3 Av. 1.84 1.26 1.22 1.88 1.96 1.41 1.512.04 BDL115 8152.4 P 0.25 0.59 0.25 0.05 0.15 0.41 BDL115 8152.4 Av.2.58 1.13 2.87 1.61 1.42 1.30 BDL115 8154.1 P 0.02 0.27 0.16 0.12 BDL1158154.1 Av. 5.48 1.16 5.83 1.36 BDL115 8155.2 P 0.05 0.24 0.20 0.13 0.800.85 BDL115 8155.2 Av. 3.97 1.36 3.99 1.33 1.13 1.25 BDL115 8155.4 P0.52 0.01 0.43 0.09 0.28 0.02 0.27 0.23 0.02 BDL115 8155.4 Av. 1.11 4.851.20 1.64 1.42 5.32 1.71 1.86 2.24 BDL116 7481.2 P 0.10 0.07 0.54 0.520.49 BDL116 7481.2 Av. 1.59 1.64 1.46 1.16 1.14 BDL116 7481.7 P 0.060.51 0.14 0.23 0.41 0.64 BDL116 7481.7 Av. 1.17 1.40 1.19 1.13 1.52 1.22BDL116 7481.8 P 0.75 0.51 0.32 0.68 0.50 0.18 0.54 BDL116 7481.8 Av.1.15 5.20 1.78 1.17 3.75 2.05 1.87 BDL116 7482.2 P 0.19 0.22 0.03 0.160.35 BDL116 7482.2 Av. 1.14 2.69 1.25 2.82 1.46 BDL116 7485.1 P 0.000.52 0.40 0.02 0.29 0.07 0.37 0.59 BDL116 7485.1 Av. 2.16 1.17 2.56 1.921.62 1.27 3.36 1.26 BDL119 7732.2 P 0.05 0.61 0.00 0.74 0.00 0.39 0.000.62 0.34 BDL119 7732.2 Av. 2.07 1.16 1.63 1.36 1.83 1.47 2.00 1.76 1.30BDL119 7733.2 P 0.42 0.46 0.11 0.15 0.07 0.01 0.73 0.32 0.10 BDL1197733.2 Av. 1.27 1.15 1.19 1.37 1.38 1.39 1.19 1.10 1.13 BDL119 7734.1 P0.23 0.07 0.01 0.01 0.00 BDL119 7734.1 Av. 1.50 1.66 1.42 1.56 1.30BDL119 7734.5 P 0.37 0.26 0.62 0.69 0.00 0.15 0.42 0.41 0.15 BDL1197734.5 Av. 1.12 1.37 1.16 1.16 1.30 1.58 1.39 1.34 1.21 BDL119 7734.7 P0.62 0.60 0.25 0.58 0.54 0.75 0.62 0.56 0.52 BDL119 7734.7 Av. 1.11 1.121.15 1.41 2.92 1.16 1.18 2.29 1.90 BDL120 7891.3 P 0.09 0.01 0.00 0.010.01 0.43 BDL120 7891.3 Av. 3.31 2.89 2.31 1.30 3.58 1.24 BDL120 7892.4P 0.23 0.62 0.12 0.31 0.53 0.48 BDL120 7892.4 Av. 1.53 1.55 1.46 1.361.94 1.14 BDL120 7892.6 P 0.39 0.60 0.59 0.58 0.69 BDL120 7892.6 Av.1.20 2.56 1.13 2.70 1.10 BDL120 7893.2 P 0.38 0.30 0.91 0.43 0.14 0.770.13 BDL120 7893.2 Av. 1.48 4.78 1.11 1.26 5.34 1.42 3.99 BDL120 7893.5P 0.02 0.46 0.60 0.08 0.61 BDL120 7893.5 Av. 1.18 1.10 2.19 1.04 2.28BDL122 7513.1 P 0.74 0.47 0.69 0.46 BDL122 7513.1 Av. 1.20 2.77 1.222.94 BDL122 7513.1 P 0.49 0.80 0.35 0.83 0.35 BDL122 7513.1 Av. 1.561.40 1.57 1.31 1.70 BDL122 7513.14 P 0.17 0.19 0.17 0.53 0.06 0.59BDL122 7513.14 Av. 1.87 1.82 1.82 1.26 2.38 1.27 BDL122 7513.9 P 0.010.46 0.52 0.00 0.44 0.52 0.45 BDL122 7513.9 Av. 1.79 2.20 4.29 1.57 3.194.97 3.28 BDL122 7514.3 P 0.22 0.15 0.58 0.57 0.01 0.04 0.61 0.65 0.55BDL122 7514.3 Av. 1.29 1.23 1.68 2.10 1.12 1.03 2.11 1.63 2.58 BDL1227513.1 P 0.17 0.09 0.01 0.02 0.12 BDL122 7513.1 Av. 1.18 1.11 4.25 4.241.89 BDL122 7513.14 P 0.50 0.88 0.74 0.54 BDL122 7513.14 Av. 1.13 1.321.97 3.21 BDL122 7513.9 P 0.31 0.35 0.86 0.31 0.37 BDL122 7513.9 Av.1.19 4.01 1.34 3.97 2.13 BDL122 7514.3 P 0.32 0.28 0.81 BDL122 7514.3Av. 2.36 2.69 1.22 BDL123 8082.1 P 0.31 0.09 0.06 0.11 0.17 BDL1238082.1 Av. 1.16 1.12 2.47 2.20 1.37 BDL123 8082.3 P 0.14 0.48 0.26 0.050.04 0.40 BDL123 8082.3 Av. 1.11 1.12 2.00 1.13 1.02 1.85 BDL123 8082.6P BDL123 8082.6 Av. BDL123 8083.2 P 0.64 0.53 0.81 0.56 0.55 0.86 0.62BDL123 8083.2 Av. 1.39 3.11 1.43 1.36 3.02 1.27 1.69 BDL123 8083.3 P0.07 0.00 0.39 0.01 0.28 BDL123 8083.3 Av. 1.37 1.32 1.77 1.15 1.75BDL124 8482.1 P 0.38 0.31 0.22 0.00 0.29 0.35 0.00 BDL124 8482.1 Av.2.25 1.48 1.85 1.50 2.12 1.54 1.20 BDL125 7491.1 P 0.21 0.06 0.20 0.15BDL125 7491.1 Av. 1.23 1.18 5.44 4.43 BDL125 7491.5 P 0.51 0.55 BDL1257491.5 Av. 1.67 1.56 BDL125 7492.5 P 0.72 0.66 0.65 BDL125 7492.5 Av.1.11 1.16 1.38 BDL125 7494.1 P 0.46 0.78 0.44 0.43 0.77 0.41 BDL1257494.1 Av. 1.41 1.12 2.37 1.24 1.13 2.53 BDL125 7495.5 P 0.36 0.43 0.020.31 0.23 BDL125 7495.5 Av. 1.22 2.05 1.34 2.25 1.54 BDL128 7711.3 P0.11 0.72 0.46 0.66 0.14 0.73 0.32 0.63 BDL128 7711.3 Av. 1.44 1.14 1.141.90 1.32 1.18 1.18 2.03 BDL128 8361.5 P 0.36 0.45 0.61 0.50 0.59 BDL1288361.5 Av. 1.24 1.80 1.14 2.15 2.09 BDL128 8362.2 P 0.10 0.09 0.10BDL128 8362.2 Av. 1.12 2.14 2.13 BDL128 8363.2 P 0.42 0.54 0.36 0.000.50 0.29 BDL128 8363.2 Av. 1.88 3.77 1.84 1.54 5.39 1.30 BDL128 8365.2P 0.41 0.47 0.42 0.47 0.48 BDL128 8365.2 Av. 1.22 2.55 1.27 3.56 3.78BDL129 7691.4 P 0.39 0.65 0.00 0.16 BDL129 7691.4 Av. 1.53 1.17 1.612.61 BDL129 7691.6 P 0.20 0.64 0.04 0.21 0.52 0.38 0.23 BDL129 7691.6Av. 1.91 1.48 1.92 1.75 1.37 2.87 1.38 BDL129 7692.2 P 0.50 0.51 0.53BDL129 7692.2 Av. 3.65 3.67 3.01 BDL129 7692.6 P 0.23 0.26 0.22 0.08BDL129 7692.6 Av. 1.12 2.87 3.20 1.17 BDL129 7693.1 P 0.35 0.47 0.000.21 0.27 0.45 0.00 0.23 0.52 BDL129 7693.1 Av. 1.50 4.24 1.22 3.15 1.434.30 1.44 4.15 3.58 BDL130 7663.1 P 0.03 0.48 BDL130 7663.1 Av. 1.021.14 BDL130 7663.3 P 0.39 0.39 0.33 0.13 0.23 0.24 0.59 0.06 BDL1307663.3 Av. 4.91 1.51 1.44 2.34 2.51 2.32 1.93 1.37 BDL130 7663.6 P 0.360.48 0.48 0.41 0.40 BDL130 7663.6 Av. 1.30 2.15 1.30 2.24 1.28 BDL1307664.5 P 0.76 0.76 0.09 BDL130 7664.5 Av. 1.17 1.20 1.06 BDL131 7461.2 P0.02 0.01 0.14 0.54 0.06 0.67 0.01 BDL131 7461.2 Av. 1.18 1.33 1.22 1.741.10 1.48 1.28 BDL131 7461.4 P 0.14 0.06 0.24 0.09 0.00 0.00 0.06 BDL1317461.4 Av. 1.12 1.14 1.11 1.08 1.05 1.04 1.02 BDL131 7462.2 P 0.00 0.080.01 0.66 0.30 0.04 0.08 0.70 BDL131 7462.2 Av. 1.38 1.43 1.25 3.42 1.121.07 1.05 2.57 BDL131 7463.4 P 0.19 0.85 0.02 0.93 BDL131 7463.4 Av.1.11 1.25 1.03 1.10 BDL131 7464.5 P 0.01 0.01 0.16 0.21 0.49 0.04 0.38BDL131 7464.5 Av. 1.26 1.19 2.44 1.29 1.17 2.63 1.15 BDL132 7471.1 P0.07 0.09 0.43 0.67 0.71 0.13 BDL132 7471.1 Av. 1.31 1.21 1.17 1.76 1.571.41 BDL132 7471.4 P 0.51 0.07 0.09 0.26 0.27 0.76 0.68 BDL132 7471.4Av. 1.11 1.20 1.13 3.00 2.64 1.19 1.20 BDL132 7472.4 P 0.48 0.94 0.480.96 BDL132 7472.4 Av. 1.33 1.18 1.27 1.12 BDL132 7473.1 P 0.00 0.390.48 0.03 0.51 0.40 BDL132 7473.1 Av. 1.27 1.15 1.70 1.02 2.06 2.66BDL132 7474.4 P 0.20 0.51 0.09 0.17 0.51 0.02 0.81 0.89 0.56 BDL1327474.4 Av. 1.74 1.92 1.20 1.74 2.41 1.29 1.16 1.15 1.87 BDL132 7471.1 P0.01 0.00 0.75 0.32 BDL132 7471.1 Av. 4.76 6.37 1.12 2.49 BDL132 7471.4P 0.08 0.20 0.61 0.11 0.00 0.18 0.16 BDL132 7471.4 Av. 5.36 1.12 1.166.92 1.49 1.50 3.72 BDL132 7472.4 P 0.05 0.04 0.54 BDL132 7472.4 Av.3.38 3.70 1.22 BDL132 7473.1 P 0.83 0.36 0.62 0.38 BDL132 7473.1 Av.1.13 1.27 1.16 1.35 BDL132 7475.4 P 0.13 0.72 0.76 0.11 0.51 BDL1327475.4 Av. 1.64 1.22 1.11 1.73 1.40 BDL133 8161.1 P 0.34 0.26 0.47 0.390.27 0.02 0.30 BDL133 8161.1 Av. 1.55 1.14 1.28 1.41 1.32 1.35 1.18BDL133 8161.2 P 0.04 0.05 0.02 0.31 0.46 0.01 0.16 0.46 0.01 0.35 BDL1338161.2 Av. 1.08 1.17 1.13 1.31 1.20 2.62 1.16 1.18 2.22 1.20 BDL1338161.3 P 0.17 0.00 0.48 0.09 0.71 0.39 0.82 0.36 BDL133 8161.3 Av. 1.111.20 7.63 1.19 1.59 2.03 1.29 2.21 BDL133 8161.4 P 0.02 0.53 0.00 0.000.04 0.01 0.20 BDL133 8161.4 Av. 1.26 1.15 1.46 1.47 1.28 1.60 1.61BDL133 8162.1 P 0.27 0.01 0.54 0.06 0.10 0.00 0.39 0.13 0.34 BDL1338162.1 Av. 1.15 1.41 1.27 1.17 1.37 1.59 1.50 1.16 1.11 BDL133 8162.3 P0.05 0.11 0.48 BDL133 8162.3 Av. 1.02 1.49 1.16 BDL133 8162.5 P 0.080.19 0.00 0.00 0.25 0.02 BDL133 8162.5 Av. 1.12 1.19 3.57 2.84 1.23 1.17BDL133 8163.2 P 0.59 0.74 0.57 0.78 0.65 BDL133 8163.2 Av. 1.53 1.261.69 1.33 1.53 BDL134 7671.2 P 0.00 0.23 0.14 0.00 0.08 0.07 0.49 0.17BDL134 7671.2 Av. 1.93 1.18 1.20 1.94 1.73 1.76 1.38 1.39 BDL134 7672.1P 0.01 0.00 0.00 0.07 0.45 0.14 0.46 BDL134 7672.1 Av. 3.86 2.37 1.491.38 1.31 1.46 1.27 BDL134 7673.1 P 0.46 0.46 0.40 0.77 0.18 0.09 0.060.06 0.17 BDL134 7673.1 Av. 11.84 1.30 1.26 1.10 2.40 2.03 2.04 1.951.14 BDL134 7673.2 P 0.19 0.39 0.26 0.01 0.18 0.11 0.16 0.00 0.62 BDL1347673.2 Av. 1.67 1.26 1.42 2.03 1.66 1.62 1.78 2.56 1.20 BDL135 7722.1 P0.51 0.34 0.66 0.07 0.45 0.43 0.64 0.24 0.10 BDL135 7722.1 Av. 1.13 1.111.85 1.14 1.17 1.11 1.86 1.25 1.11 BDL135 7723.1 P 0.02 0.24 BDL1357723.1 Av. 1.02 1.22 BDL135 7723.3 P 0.43 0.27 0.51 0.06 0.29 0.02BDL135 7723.3 Av. 1.13 5.05 1.20 1.29 6.62 1.34 BDL135 7723.8 P 0.720.61 0.35 0.26 BDL135 7723.8 Av. 1.12 1.13 1.10 1.13 BDL135 7723.9 P0.03 0.12 0.05 0.17 0.04 0.29 BDL135 7723.9 Av. 1.19 1.15 1.12 1.16 1.031.21 BDL136 7751.4 P 0.19 0.57 0.19 0.48 BDL136 7751.4 Av. 1.26 1.181.21 1.19 BDL136 7751.5 P 0.53 0.75 0.53 0.39 0.44 0.44 0.36 0.01 BDL1367751.5 Av. 1.57 1.12 1.40 1.49 1.35 1.41 1.77 1.12 BDL136 7751.8 P 0.030.02 0.05 0.05 0.10 0.39 BDL136 7751.8 Av. 1.20 1.23 1.31 1.08 1.02 1.14BDL136 7752.6 P 0.12 BDL136 7752.6 Av. 1.35 BDL137 7701.2 P 0.21 0.050.50 0.06 0.47 0.40 0.05 BDL137 7701.2 Av. 1.15 1.14 5.92 1.02 1.90 2.371.21 BDL137 7701.5 P 0.61 0.73 0.47 0.68 0.68 BDL137 7701.5 Av. 1.281.41 1.23 1.57 1.12 BDL137 7701.6 P 0.84 0.75 0.73 0.76 0.01 0.14 BDL1377701.6 Av. 1.10 1.42 1.15 1.41 1.30 1.13 BDL137 7702.1 P 0.19 0.14 0.250.02 0.10 0.00 0.15 0.00 BDL137 7702.1 Av. 2.42 1.29 1.38 1.95 1.94 1.492.00 1.42 BDL137 7703.2 P 0.41 0.45 0.59 0.35 0.41 0.46 0.61 0.30 BDL1377703.2 Av. 1.55 1.52 2.47 1.15 1.50 1.59 2.22 1.27 BDL137 7703.3 P 0.320.01 0.18 0.30 0.52 0.19 BDL137 7703.3 Av. 1.19 1.25 1.23 1.42 1.20 1.27BDL137 7703.7 P 0.53 0.55 0.53 0.53 0.41 0.17 BDL137 7703.7 Av. 1.121.45 1.16 1.18 1.42 1.32 BDL139 8131.1 P BDL139 8131.1 Av. BDL139 8131.2P 0.12 0.44 0.10 0.83 0.00 0.27 0.00 0.64 0.38 0.50 BDL139 8131.2 Av.1.70 1.21 1.21 1.19 1.79 1.78 1.71 1.64 1.20 1.18 BDL139 8132.7 P BDL1398132.7 Av. BDL139 8133.2 P BDL139 8133.2 Av. BDL141 8141.2 P 0.30 0.01BDL141 8141.2 Av. 1.12 1.13 BDL141 8142.2 P BDL141 8142.2 Av. BDL1428282.1 P 0.43 0.35 0.52 0.15 0.27 0.38 0.42 0.13 0.01 BDL142 8282.1 Av.1.17 1.21 1.20 1.47 1.31 1.37 1.33 1.49 1.25 BDL142 8283.1 P 0.27 0.260.14 0.03 0.65 0.24 0.33 BDL142 8283.1 Av. 4.32 1.94 2.12 1.30 1.17 2.041.10 BDL142 8283.2 P 0.04 0.01 0.30 BDL142 8283.2 Av. 1.07 1.14 1.42BDL142 8284.1 P 0.68 0.35 0.64 0.85 0.29 0.17 BDL142 8284.1 Av. 1.181.20 1.23 1.11 1.21 1.13 BDL142 8285.3 P 0.21 0.60 0.14 0.45 0.61 0.560.22 0.03 BDL142 8285.3 Av. 2.01 1.50 1.82 1.21 1.28 1.66 1.40 1.16BDL142 8285.5 P 0.52 0.65 0.36 BDL142 8285.5 Av. 1.33 1.26 1.29 BDL1438411.1 P 0.00 0.00 0.11 0.15 0.00 0.02 0.02 0.06 BDL143 8411.1 Av. 1.281.24 1.41 1.17 1.05 1.02 1.03 1.17 BDL143 8411.5 P 0.91 0.17 BDL1438411.5 Av. 1.24 1.11 BDL143 8412.2 P 0.31 0.43 0.09 0.77 0.00 0.24 0.130.69 0.02 BDL143 8412.2 Av. 1.22 1.24 1.49 1.35 1.31 1.51 1.63 1.52 1.34BDL143 8412.4 P 0.23 0.63 0.59 0.03 0.42 0.28 0.78 BDL143 8412.4 Av.1.14 1.21 1.26 1.80 1.90 2.17 1.22 BDL143 8413.3 P 0.09 0.42 0.03 0.430.00 0.27 0.00 0.31 0.44 BDL143 8413.3 Av. 1.69 1.28 1.29 1.40 1.61 1.501.55 1.64 1.20 BDL143 8414.4 P 0.34 0.68 0.73 0.66 0.07 0.67 BDL1438414.4 Av. 1.16 1.72 1.17 1.13 1.25 1.68 BDL143 8414.5 P 0.35 0.60 0.200.26 0.40 0.30 0.29 BDL143 8414.5 Av. 1.23 1.13 1.19 1.28 1.38 1.40 1.13BDL144 8384.1 P 0.09 0.35 0.10 0.37 0.36 BDL144 8384.1 Av. 1.24 1.181.23 1.17 1.35 BDL144 8384.5 P 0.36 0.13 BDL144 8384.5 Av. 1.18 1.24BDL144 8385.1 P 0.00 0.07 0.39 0.73 0.00 0.05 0.00 0.06 0.13 BDL1448385.1 Av. 2.50 1.35 1.25 1.14 2.06 2.23 1.86 1.71 1.50 BDL145 8233.2 P0.25 0.00 0.73 0.08 BDL145 8233.2 Av. 1.47 1.47 1.24 1.15 BDL145 8233.3P 0.37 0.45 0.58 0.08 0.06 0.30 0.48 0.39 0.23 BDL145 8233.3 Av. 9.001.13 1.21 2.55 1.79 1.75 1.51 1.35 1.36 BDL145 8235.1 P 0.36 0.41 0.220.32 0.79 0.32 BDL145 8235.1 Av. 1.25 1.57 1.27 1.73 1.34 1.16 BDL1458235.3 P 0.21 0.16 0.65 0.02 0.14 0.00 0.43 0.41 0.38 BDL145 8235.3 Av.3.39 1.17 1.35 2.32 1.87 1.92 2.33 1.31 1.21 BDL145 8235.4 P BDL1458235.4 Av. BDL146 8241.1 P 0.29 0.42 0.72 0.32 0.30 BDL146 8241.1 Av.1.18 1.14 1.11 1.10 1.37 BDL146 8241.3 P 0.10 0.11 0.31 0.01 0.45 0.010.42 0.04 BDL146 8241.3 Av. 1.19 1.29 1.88 1.26 1.24 1.41 1.96 1.08BDL146 8243.2 P 0.62 0.72 0.59 0.15 0.05 BDL146 8243.2 Av. 1.52 1.121.42 1.18 1.16 BDL146 8243.5 P 0.50 0.59 0.68 0.32 0.56 0.59 0.62 0.70BDL146 8243.5 Av. 1.42 1.20 1.13 1.46 1.47 1.34 1.31 1.11 BDL146 8244.4P 0.29 0.21 0.33 0.77 0.01 0.89 BDL146 8244.4 Av. 1.15 1.17 1.30 1.391.04 1.17 BDL146 8244.7 P 0.07 0.00 0.01 0.29 0.01 0.00 0.00 0.18 0.47BDL146 8244.7 Av. 1.74 1.58 1.42 1.41 1.66 2.03 1.77 1.97 1.26 BDL1468245.2 P 0.69 0.50 0.43 0.35 0.59 0.47 BDL146 8245.2 Av. 1.21 3.35 1.111.12 1.30 3.30 BDL146 8245.5 P 0.02 0.67 0.08 0.71 0.00 BDL146 8245.5Av. 1.13 2.48 1.07 2.05 1.25 BDL42 7771.1 P BDL42 7771.1 Av. BDL427772.1 P BDL42 7772.1 Av. BDL42 7772.7 P BDL42 7772.7 Av. BDL42 7774.1 P0.01 0.15 0.35 0.73 0.00 0.00 0.15 0.01 BDL42 7774.1 Av. 4.17 1.16 1.251.11 2.40 2.04 2.29 2.15 BDL42 7774.2 P BDL42 7774.2 Av. BDL42 7774.4 P0.43 0.31 0.73 0.24 0.06 0.00 0.12 0.46 BDL42 7774.4 Av. 3.46 1.18 1.102.06 1.55 1.85 2.09 1.15 BDL46 7833.3 P 0.02 0.70 0.33 0.47 0.00 0.340.09 0.43 0.31 BDL46 7833.3 Av. 1.53 1.11 1.19 4.71 1.55 1.52 1.51 6.071.24 BDL46 7833.4 P 0.02 0.02 BDL46 7833.4 Av. 1.04 1.17 BDL46 7833.5 P0.49 0.43 BDL46 7833.5 Av. 1.55 1.44 BDL46 7833.6 P 0.12 0.54 0.52 0.330.53 0.50 BDL46 7833.6 Av. 1.24 1.74 3.06 1.18 1.94 3.78 BDL46 7834.1 PBDL46 7834.1 Av. BDL46 7833.1 P 0.81 0.15 0.06 0.37 0.80 0.25 0.00 0.04BDL46 7833.1 Av. 2.17 1.81 1.26 1.29 2.21 2.29 1.66 1.76 BDL46 7833.3 P0.75 0.30 0.00 0.79 BDL46 7833.3 Av. 1.13 1.50 1.41 1.44 BDL46 7833.4 PBDL46 7833.4 Av. BDL46 7833.5 P 0.54 0.25 0.09 BDL46 7833.5 Av. 1.221.77 1.68 BDL46 7834.4 P 0.25 0.00 0.13 0.04 0.41 0.00 0.04 0.00 BDL467834.4 Av. 2.23 3.05 2.50 3.07 1.88 3.45 2.95 3.50 BDL51 7291.1 P 0.170.34 0.24 0.07 0.06 0.22 0.13 0.04 0.10 BDL51 7291.1 Av. 2.46 1.84 1.593.10 2.14 2.30 2.12 4.63 1.11 BDL51 8021.1 P 0.00 0.58 0.03 0.50 BDL518021.1 Av. 2.43 3.13 4.73 7.50 BDL51 8022.4 P 0.02 0.31 0.63 0.02 0.250.16 0.49 BDL51 8022.4 Av. 2.50 1.54 1.30 4.06 3.15 3.24 1.29 BDL518022.5 P 0.28 0.31 0.21 0.15 0.16 0.15 0.12 0.14 BDL51 8022.5 Av. 3.272.22 1.66 2.02 2.40 3.77 4.00 5.31 BDL51 8024.4 P 0.23 0.06 0.32 0.550.10 0.12 0.30 0.51 0.85 BDL51 8024.4 Av. 2.16 1.60 1.55 3.05 1.80 2.192.27 4.90 1.14 BDL51 8024.7 P 0.40 0.45 0.29 0.79 BDL51 8024.7 Av. 1.501.12 1.71 1.12 BDL52 7861.1 P 0.64 0.68 BDL52 7861.1 Av. 1.20 1.15 BDL527861.5 P 0.38 0.03 0.38 0.04 0.01 BDL52 7861.5 Av. 1.28 2.63 1.19 2.581.16 BDL52 7863.2 P BDL52 7863.2 Av. BDL52 7864.5 P 0.16 0.63 0.62 BDL527864.5 Av. 1.12 1.30 1.14 BDL54 7781.1 P 0.51 0.51 0.51 BDL54 7781.1 Av.4.05 3.55 2.68 BDL54 7781.4 P 0.18 0.29 0.72 BDL54 7781.4 Av. 1.67 1.571.15 BDL54 7784.3 P 0.21 0.43 0.17 0.31 0.29 BDL54 7784.3 Av. 2.32 2.782.05 3.64 2.55 BDL54 7784.5 P 0.11 0.40 0.01 0.27 0.35 0.18 BDL54 7784.5Av. 1.38 3.91 1.29 1.23 4.08 1.29 BDL54 7785.4 P 0.00 0.00 0.02 BDL547785.4 Av. 1.70 1.56 1.23 BDL54 7781.1 P 0.08 0.78 0.25 0.59 0.52 0.23BDL54 7781.1 Av. 3.23 1.30 3.98 1.88 1.41 1.72 BDL54 7781.4 P 0.55 0.540.24 0.92 BDL54 7781.4 Av. 3.16 3.57 1.52 1.12 BDL54 7784.3 P 0.52 0.17BDL54 7784.3 Av. 1.21 1.51 BDL54 7785.4 P 0.04 0.53 0.13 0.11 0.34 BDL547785.4 Av. 6.70 1.20 10.24 1.62 3.57 BDL54 7785.8 P 0.14 0.37 0.16 0.330.00 BDL54 7785.8 Av. 6.30 1.51 8.24 1.48 3.47 BDL56 7181.2 P 0.15 0.210.73 0.52 0.14 0.26 0.66 0.50 0.46 BDL56 7181.2 Av. 1.26 1.91 1.41 2.391.44 2.65 1.64 3.57 2.09 BDL56 8301.1 P 0.00 0.05 0.11 0.55 0.32 0.010.03 0.66 BDL56 8301.1 Av. 1.28 1.27 1.22 1.82 1.13 1.04 1.03 1.45 BDL568301.3 P 0.40 0.81 0.35 0.75 BDL56 8301.3 Av. 2.62 1.11 2.83 1.15 BDL568304.1 P 0.38 BDL56 8304.1 Av. 1.11 BDL56 8305.1 P 0.38 0.43 0.07 0.460.79 BDL56 8305.1 Av. 1.19 1.52 1.17 1.58 1.12 BDL56 8301.1 P 0.29 0.00BDL56 8301.1 Av. 1.27 1.63 BDL56 8301.2 P 0.77 BDL56 8301.2 Av. 1.68BDL56 8301.3 P 0.54 0.95 0.91 0.84 0.36 BDL56 8301.3 Av. 1.25 1.36 1.621.11 1.80 BDL56 8303.1 P BDL56 8303.1 Av. BDL56 8303.2 P 0.53 0.01 0.330.00 0.08 0.51 0.12 BDL56 8303.2 Av. 1.11 6.56 1.13 9.42 1.25 1.22 5.45BDL59 7792.1 P 0.39 0.07 0.50 BDL59 7792.1 Av. 1.69 1.08 1.58 BDL597792.2 P 0.69 0.68 BDL59 7792.2 Av. 2.56 2.78 BDL59 7792.3 P 0.16 0.05BDL59 7792.3 Av. 1.17 1.03 BDL59 7793.3 P 0.64 0.83 0.57 0.73 0.36 BDL597793.3 Av. 1.21 1.15 1.26 1.24 1.35 BDL59 7794.1 P 0.27 0.86 0.17 0.810.00 BDL59 7794.1 Av. 2.60 1.13 3.67 1.18 3.34 BDL60 8011.4 P 0.45 0.45BDL60 8011.4 Av. 1.13 1.12 BDL60 8011.7 P 0.00 0.48 0.01 0.44 0.41 0.45BDL60 8011.7 Av. 2.02 2.83 1.98 3.86 1.35 2.63 BDL60 8013.4 P 0.08 0.400.01 0.05 0.27 BDL60 8013.4 Av. 2.96 2.37 2.30 1.57 3.52 BDL60 8013.6 P0.21 0.49 0.00 0.70 0.04 0.47 0.26 BDL60 8013.6 Av. 1.96 6.84 1.85 1.111.20 8.09 1.42 BDL60 8014.5 P 0.36 0.90 0.23 0.62 0.76 0.70 BDL60 8014.5Av. 2.07 1.16 1.84 1.19 1.29 1.76 BDL60 8013.6 P 0.01 0.34 0.32 0.210.00 0.04 0.19 0.09 0.77 0.89 BDL60 8013.6 Av. 6.90 1.59 1.30 2.17 8.761.88 1.59 2.45 1.23 1.17 BDL60 8014.2 P 0.04 0.18 0.01 0.00 0.16 0.00BDL60 8014.2 Av. 1.58 1.50 2.12 2.04 2.11 3.09 BDL60 8014.7 P 0.56 0.430.42 0.45 0.50 BDL60 8014.7 Av. 1.55 1.26 1.23 1.29 2.00 BDL60 8014.8 P0.81 0.12 0.16 0.66 0.17 0.25 0.24 0.41 BDL60 8014.8 Av. 1.12 7.59 1.181.14 9.76 1.41 1.39 4.18 BDL65 7824.1 P 0.57 0.36 0.65 0.41 0.01 0.550.47 0.03 BDL65 7824.1 Av. 1.51 1.24 1.37 1.51 1.72 1.21 2.26 1.36 BDL657825.2 P 0.40 0.49 0.11 0.17 0.21 0.15 BDL65 7825.2 Av. 1.29 1.18 1.761.49 1.45 2.51 BDL65 8473.2 P 0.13 0.20 0.07 0.51 0.01 0.05 0.00 0.420.13 BDL65 8473.2 Av. 3.11 1.16 1.31 2.00 2.17 1.78 1.82 2.80 1.34 BDL658474.1 P 0.13 0.22 0.05 0.33 0.64 BDL65 8474.1 Av. 1.25 1.78 1.28 2.011.12 BDL67 7901.5 P 0.38 0.26 0.15 0.17 0.36 0.18 0.23 0.12 BDL67 7901.5Av. 1.56 1.22 1.29 1.92 1.46 1.34 1.48 2.49 BDL67 7902.3 P 0.46 0.650.47 0.68 0.48 0.49 BDL67 7902.3 Av. 1.12 1.11 2.65 1.11 3.06 2.43 BDL677902.7 P 0.19 0.54 0.20 0.51 0.66 0.55 BDL67 7902.7 Av. 1.49 1.94 1.462.91 1.15 2.69 BDL67 7903.3 P 0.68 0.73 0.71 0.68 BDL67 7903.3 Av. 1.191.99 1.17 2.50 BDL67 7903.5 P 0.12 0.54 0.00 0.00 0.05 0.49 0.04 BDL677903.5 Av. 2.41 2.43 2.03 1.41 1.33 2.85 1.26 BDL68 7761.3 P 0.59 0.740.59 0.66 0.72 BDL68 7761.3 Av. 1.42 1.55 1.32 1.17 1.60 BDL68 7761.8 P0.45 0.21 0.23 0.00 0.29 0.04 0.09 BDL68 7761.8 Av. 7.80 1.77 2.62 2.041.30 2.56 2.02 BDL68 7761.9 P 0.39 0.64 0.96 0.63 BDL68 7761.9 Av. 1.601.67 1.12 1.41 BDL68 7763.2 P 0.24 0.30 0.19 0.14 0.46 BDL68 7763.2 Av.2.08 1.62 1.91 1.99 1.28 BDL68 7764.1 P 0.42 0.54 0.83 0.13 0.46 0.530.45 0.40 BDL68 7764.1 Av. 9.83 2.45 1.14 2.76 3.43 1.26 1.50 2.71 BDL787911.11 P 0.40 0.08 0.56 0.14 0.00 BDL78 7911.11 Av. 1.11 1.16 1.14 1.211.24 BDL78 7911.8 P 0.63 0.15 0.57 0.60 0.36 BDL78 7911.8 Av. 1.79 1.211.22 2.17 1.30 BDL78 7911.9 P 0.20 0.00 0.14 0.00 0.69 0.00 BDL78 7911.9Av. 6.36 2.58 1.94 1.88 1.25 1.48 BDL78 7912.6 P 0.70 0.61 0.63 0.37BDL78 7912.6 Av. 1.21 1.14 1.28 1.16 BDL78 7913.11 P 0.24 0.66 0.05 0.320.24 0.13 0.67 BDL78 7913.11 Av. 1.72 1.13 1.84 1.18 1.50 1.60 1.11BDL78 7913.3 P 0.53 0.44 0.00 BDL78 7913.3 Av. 1.14 1.20 1.31 BDL787913.6 P 0.45 0.25 0.49 0.68 0.18 0.12 0.06 0.24 0.49 0.42 BDL78 7913.6Av. 8.48 1.13 1.22 1.23 2.37 1.90 1.81 1.92 1.16 1.44 BDL78 7913.8 P0.31 0.21 0.68 0.04 0.23 0.10 0.53 0.07 0.48 BDL78 7913.8 Av. 1.36 1.281.29 1.30 1.69 1.58 1.69 1.14 1.11 BDL78 7913.9 P 0.51 0.52 0.20 0.340.49 0.46 0.15 0.07 BDL78 7913.9 Av. 1.37 1.14 1.41 1.48 1.25 1.28 1.501.53 BDL82 7801.1 P 0.20 0.00 0.24 0.34 0.00 0.00 0.12 0.25 BDL82 7801.1Av. 2.67 1.39 1.66 2.12 2.04 1.80 2.22 3.05 BDL82 7801.3 P 0.33 0.830.03 0.03 0.14 BDL82 7801.3 Av. 8.14 1.12 2.90 1.28 2.08 BDL82 7802.2 P0.18 0.44 0.14 0.77 0.01 0.41 0.09 BDL82 7802.2 Av. 2.55 5.01 2.16 1.111.26 6.53 1.35 BDL82 7802.3 P 0.16 0.67 0.00 0.43 0.08 0.48 0.21 0.06BDL82 7802.3 Av. 2.36 1.21 1.20 1.58 2.03 1.70 1.72 2.45 BDL82 7803.9 P0.24 0.01 0.37 0.00 0.00 0.46 BDL82 7803.9 Av. 1.15 1.30 1.18 1.14 1.041.28 BDL89 7812.2 P 0.49 0.67 0.34 BDL89 7812.2 Av. 1.15 1.14 1.11 BDL897812.5 P 0.44 0.55 0.26 0.45 0.46 0.29 0.49 BDL89 7812.5 Av. 3.98 1.467.37 3.87 1.49 7.29 2.55 BDL89 7814.1 P 0.03 0.20 0.29 0.44 0.42 0.490.45 0.43 0.77 BDL89 7814.1 Av. 1.22 1.11 1.17 2.98 1.12 1.12 1.14 2.971.11 BDL89 7814.4 P 0.51 0.42 0.00 0.44 0.51 0.39 0.15 0.10 0.33 BDL897814.4 Av. 3.96 2.15 1.23 1.63 1.94 3.04 1.58 2.16 1.96 BDL89 7814.5 P0.42 0.55 0.43 0.31 0.20 0.42 0.19 0.32 0.13 BDL89 7814.5 Av. 3.45 1.141.67 2.25 1.16 1.45 2.21 1.39 1.12 Table 26.

TABLE 27 Gene Ev. Par. 35 36 37 38 39 40 41 42 43 44 45 BDL95 7841.5 P0.67 0.70 0.74 0.55 0.14 0.74 BDL95 7841.5 Av. 1.64 1.36 1.22 1.11 1.181.22 BDL95 7842.12 P 0.77 0.03 BDL95 7842.12 Av. 1.12 1.19 BDL95 7842.2P 0.71 0.58 BDL95 7842.2 Av. 1.14 1.11 BDL95 7842.8 P 0.28 0.58 0.780.01 0.00 BDL95 7842.8 Av. 1.18 1.14 1.11 1.21 1.29 BDL95 7843.4 P 0.490.49 0.49 BDL95 7843.4 Av. 10.16 5.89 9.51 BDL100 7871.2 P 0.39 0.360.37 0.08 0.21 BDL100 7871.2 Av. 1.13 1.25 1.14 1.25 1.15 BDL100 7872.2P 0.03 0.29 0.46 0.26 0.32 0.09 BDL100 7872.2 Av. 1.40 1.25 1.40 1.201.40 1.20 BDL100 7872.3 P 0.01 BDL100 7872.3 Av. 1.49 BDL100 7873.3 P0.42 0.03 0.02 BDL100 7873.3 Av. 1.18 1.16 1.23 BDL100 7873.4 P 0.500.01 0.43 BDL100 7873.4 Av. 2.93 1.37 2.58 BDL106 7881.1 P 0.01 0.500.00 0.00 BDL106 7881.1 Av. 1.41 1.39 1.40 1.40 BDL106 7881.4 P 0.470.06 0.68 0.68 BDL106 7881.4 Av. 8.12 1.69 1.12 1.12 BDL106 7882.6 P0.63 0.25 0.04 BDL106 7882.6 Av. 1.42 1.19 1.15 BDL106 7884.1 P 0.490.50 0.51 0.16 BDL106 7884.1 Av. 12.26 5.79 4.04 1.36 BDL106 7884.9 P0.54 0.31 0.69 0.47 0.62 0.24 0.69 BDL106 7884.9 Av. 3.90 2.59 1.32 1.131.12 1.19 1.32 BDL106 7881.1 P 0.44 0.00 0.46 0.01 0.23 0.21 0.01 BDL1067881.1 Av. 1.19 1.37 1.49 1.15 1.16 1.21 1.15 BDL106 7881.2 P 0.74 0.290.40 0.62 0.40 BDL106 7881.2 Av. 1.17 1.69 1.16 1.21 1.16 BDL106 7882.2P 0.11 0.73 BDL106 7882.2 Av. 1.30 1.54 BDL106 7882.4 P 0.08 0.24 0.600.09 BDL106 7882.4 Av. 2.00 1.12 1.22 1.25 BDL106 7882.5 P 0.14 0.440.43 0.26 0.06 0.03 BDL106 7882.5 Av. 1.30 1.72 1.21 1.12 1.39 1.16BDL108 8122.2 P 0.05 0.62 0.48 0.58 0.49 0.39 0.48 0.25 BDL108 8122.2Av. 1.15 3.47 4.92 1.26 4.63 1.16 2.58 1.40 BDL108 8122.3 P 0.42 0.530.44 0.31 0.44 0.05 0.44 BDL108 8122.3 Av. 4.07 1.46 1.37 1.13 1.22 1.171.37 BDL108 8123.1 P 0.14 0.78 0.03 0.37 0.40 0.03 BDL108 8123.1 Av.3.19 1.13 1.93 1.25 1.22 1.93 BDL108 8123.2 P 0.59 0.51 0.39 0.71 0.520.50 0.54 0.38 0.71 BDL108 8123.2 Av. 6.61 11.57 2.13 2.17 6.48 1.303.24 1.56 2.17 BDL108 8123.5 P 0.45 0.22 0.47 0.13 0.43 0.47 BDL1088123.5 Av. 4.40 1.30 1.34 1.11 1.30 1.34 BDL108 8121.1 P 0.21 BDL1088121.1 Av. 3.08 BDL108 8121.3 P 0.01 BDL108 8121.3 Av. 1.65 BDL1088121.4 P 0.76 0.06 BDL108 8121.4 Av. 1.23 1.44 BDL108 8122.7 P 0.02BDL108 8122.7 Av. 1.90 BDL108 8123.7 P 0.48 0.34 0.09 0.74 0.00 BDL1088123.7 Av. 1.21 1.15 1.58 1.11 1.21 BDL110 8092.1 P 0.24 BDL110 8092.1Av. 1.18 BDL110 8092.2 P 0.11 0.01 0.01 BDL110 8092.2 Av. 1.75 1.20 1.24BDL110 8092.5 P 0.27 0.04 BDL110 8092.5 Av. 1.17 1.09 BDL110 8095.2 P0.61 BDL110 8095.2 Av. 1.11 BDL111 8102.7 P 0.24 0.54 0.14 0.04 0.020.21 BDL111 8102.7 Av. 1.33 1.24 1.81 1.24 1.30 1.13 BDL111 8103.1 P0.68 0.09 0.04 0.20 0.00 0.07 BDL111 8103.1 Av. 1.37 1.70 1.77 1.54 1.371.15 BDL111 8103.2 P 0.26 0.72 0.76 0.72 BDL111 8103.2 Av. 2.29 1.131.14 1.13 BDL111 8103.4 P 0.52 0.53 0.08 0.47 BDL111 8103.4 Av. 2.011.22 1.81 1.19 BDL111 8103.5 P 0.25 0.40 0.17 0.67 0.64 0.27 0.03 0.67BDL111 8103.5 Av. 1.87 1.19 1.71 1.20 1.12 1.13 1.20 1.20 BDL111 8102.7P 0.31 0.52 0.44 0.27 BDL111 8102.7 Av. 1.32 1.29 1.12 1.23 BDL1118103.1 P 0.45 0.24 0.00 BDL111 8103.1 Av. 1.24 1.12 1.31 BDL111 8103.2 P0.61 0.51 0.33 0.25 BDL111 8103.2 Av. 1.93 1.80 1.25 1.22 BDL111 8103.4P 0.15 0.25 0.40 BDL111 8103.4 Av. 1.66 1.18 1.17 BDL111 8103.5 P 0.150.34 0.10 BDL111 8103.5 Av. 1.80 1.72 1.11 BDL112 7502.1 P 0.07 0.380.09 BDL112 7502.1 Av. 1.30 1.22 1.14 BDL112 7502.14 P 0.50 0.49 0.49BDL112 7502.14 Av. 9.53 4.98 9.33 BDL112 7502.4 P 0.40 0.33 0.02 0.650.48 0.00 0.03 0.65 BDL112 7502.4 Av. 2.81 1.20 1.35 1.37 1.31 1.23 1.191.37 BDL112 7502.7 P 0.26 0.31 0.45 0.00 0.20 BDL112 7502.7 Av. 2.001.76 1.21 1.22 1.15 BDL112 7502.9 P 0.59 0.48 0.28 0.15 BDL112 7502.9Av. 1.14 1.29 1.15 1.11 BDL112 7502.1 P 0.31 0.39 0.03 0.04 0.02 BDL1127502.1 Av. 2.00 1.38 1.11 1.38 1.26 BDL112 7502.4 P 0.01 0.22 0.01 0.020.26 0.43 0.01 BDL112 7502.4 Av. 1.26 1.85 1.15 1.13 1.29 1.19 1.15BDL112 7502.7 P 0.25 0.11 0.20 0.41 0.00 0.25 0.57 0.41 BDL112 7502.7Av. 1.28 1.50 1.20 1.11 1.23 1.30 1.10 1.11 BDL112 7502.8 P 0.38 0.490.58 0.01 0.01 0.07 0.15 0.01 0.01 BDL112 7502.8 Av. 1.21 1.64 1.17 1.141.15 1.16 1.21 1.25 1.14 BDL112 7502.9 P 0.06 0.07 0.11 0.00 BDL1127502.9 Av. 1.22 1.63 1.12 1.34 BDL113 7683.4 P 0.29 0.06 0.28 0.01 0.020.07 0.28 BDL113 7683.4 Av. 2.64 2.49 1.88 1.23 1.11 1.15 1.88 BDL1137683.6 P 0.30 0.24 0.00 0.03 0.33 BDL113 7683.6 Av. 2.33 1.40 1.67 1.471.21 BDL113 7684.3 P 0.68 0.14 0.00 BDL113 7684.3 Av. 1.42 1.21 1.92BDL113 7684.6 P 0.25 0.21 0.70 0.51 0.37 0.70 BDL113 7684.6 Av. 2.211.55 1.20 1.12 1.10 1.20 BDL113 7684.7 P 0.02 0.32 BDL113 7684.7 Av.1.67 1.15 BDL113 7683.1 P 0.05 0.43 0.54 0.41 0.01 BDL113 7683.1 Av.1.39 1.62 1.15 1.20 1.20 BDL113 7683.11 P BDL113 7683.11 Av. BDL1137683.4 P 0.72 0.77 0.69 0.39 0.00 BDL113 7683.4 Av. 1.33 1.21 1.12 1.141.24 BDL113 7684.1 P BDL113 7684.1 Av. BDL113 7684.5 P BDL113 7684.5 Av.BDL114 7741.3 P 0.50 0.00 0.50 0.10 0.49 0.33 BDL114 7741.3 Av. 24.741.73 10.33 1.14 6.56 1.14 BDL114 7741.6 P 0.50 0.49 0.50 BDL114 7741.6Av. 65.41 11.28 9.24 BDL114 7742.1 P 0.50 0.39 0.01 0.20 BDL114 7742.1Av. 1.51 1.22 1.16 1.11 BDL114 7742.3 P 0.48 0.00 0.03 0.15 BDL1147742.3 Av. 6.61 1.94 1.69 1.30 BDL114 7742.5 P 0.22 0.29 0.02 0.45 0.170.02 0.34 BDL114 7742.5 Av. 3.37 1.93 2.22 1.58 1.14 1.57 1.32 BDL1158152.3 P 0.27 BDL115 8152.3 Av. 1.28 BDL115 8152.4 P 0.05 0.00 0.02 0.070.02 BDL115 8152.4 Av. 1.42 1.67 1.58 1.12 1.58 BDL115 8154.1 P 0.150.11 0.40 0.22 BDL115 8154.1 Av. 1.41 1.31 1.32 1.23 BDL115 8155.2 P0.49 0.03 0.50 0.50 BDL115 8155.2 Av. 9.95 1.32 6.80 12.32 BDL115 8155.4P 0.72 0.49 0.00 0.51 0.45 BDL115 8155.4 Av. 2.26 4.22 1.60 3.59 1.83BDL115 8152.3 P 0.51 0.24 0.01 0.12 0.33 BDL115 8152.3 Av. 1.56 1.841.27 1.22 1.13 BDL115 8152.4 P 0.01 0.57 0.01 0.25 0.08 0.08 0.02 BDL1158152.4 Av. 2.40 1.29 2.01 1.20 1.19 1.63 1.25 BDL115 8154.1 P 0.35 0.850.00 0.29 0.01 0.40 0.42 0.29 BDL115 8154.1 Av. 1.78 1.15 2.51 1.19 1.161.47 1.14 1.19 BDL115 8155.2 P 0.11 BDL115 8155.2 Av. 1.60 BDL115 8155.4P 0.05 BDL115 8155.4 Av. 2.30 BDL116 7481.2 P 0.24 0.16 0.18 0.12 0.22BDL116 7481.2 Av. 1.61 1.19 1.16 1.21 1.17 BDL116 7481.7 P 0.48 0.690.26 0.74 0.44 0.63 0.74 BDL116 7481.7 Av. 1.11 1.11 1.44 1.14 1.13 1.111.14 BDL116 7481.8 P 0.49 0.50 0.49 0.33 BDL116 7481.8 Av. 15.78 5.3313.31 1.10 BDL116 7482.2 P 0.45 0.10 0.46 0.02 0.13 0.46 BDL116 7482.2Av. 1.23 1.53 1.34 1.18 1.22 1.34 BDL116 7485.1 P 0.00 0.13 0.36 0.460.08 0.55 0.20 BDL116 7485.1 Av. 2.03 1.35 1.47 1.27 1.20 1.10 1.31BDL119 7732.2 P 0.09 0.03 0.09 0.10 BDL119 7732.2 Av. 2.30 1.61 1.161.44 BDL119 7733.2 P 0.70 0.01 0.23 0.06 0.27 BDL119 7733.2 Av. 1.121.47 1.28 1.21 1.39 BDL119 7734.1 P 0.30 0.02 0.52 0.21 0.15 BDL1197734.1 Av. 1.21 1.50 1.12 1.13 1.36 BDL119 7734.5 P 0.39 BDL119 7734.5Av. 1.16 BDL119 7734.7 P BDL119 7734.7 Av. BDL120 7891.3 P 0.00 0.060.15 0.02 0.35 0.10 0.15 BDL120 7891.3 Av. 5.30 1.42 1.84 1.19 1.30 1.131.84 BDL120 7892.4 P 0.36 0.01 0.03 0.00 0.26 BDL120 7892.4 Av. 1.371.48 1.28 1.37 1.15 BDL120 7892.6 P BDL120 7892.6 Av. BDL120 7893.2 P0.65 0.36 0.86 0.19 0.14 BDL120 7893.2 Av. 1.11 22.46 1.20 11.26 1.13BDL120 7893.5 P 0.39 0.22 0.22 BDL120 7893.5 Av. 1.13 1.19 1.19 BDL1227513.1 P 0.16 BDL122 7513.1 Av. 1.20 BDL122 7513.1 P 0.17 0.48 0.06 0.760.30 0.06 BDL122 7513.1 Av. 1.12 1.90 1.51 1.13 1.26 1.51 BDL122 7513.14P 0.23 0.31 0.00 0.02 BDL122 7513.14 Av. 2.17 1.18 1.27 1.30 BDL1227513.9 P 0.49 0.48 0.45 BDL122 7513.9 Av. 5.96 5.25 3.93 BDL122 7514.3 P0.53 0.53 0.42 BDL122 7514.3 Av. 4.10 4.19 1.34 BDL122 7513.1 P 0.44BDL122 7513.1 Av. 1.17 BDL122 7513.14 P 0.36 0.42 0.04 0.04 BDL1227513.14 Av. 1.28 1.17 1.26 1.26 BDL122 7513.9 P BDL122 7513.9 Av. BDL1227514.3 P 0.34 BDL122 7514.3 Av. 1.73 BDL123 8082.1 P 0.00 BDL123 8082.1Av. 1.30 BDL123 8082.3 P BDL123 8082.3 Av. BDL123 8082.6 P 0.72 0.380.68 0.08 BDL123 8082.6 Av. 1.21 1.45 1.17 1.15 BDL123 8083.2 P 0.500.50 0.50 BDL123 8083.2 Av. 20.40 9.06 17.24 BDL123 8083.3 P BDL1238083.3 Av. BDL124 8482.1 P 0.17 0.39 0.04 0.01 0.09 BDL124 8482.1 Av.1.12 2.21 1.35 1.12 1.18 BDL125 7491.1 P BDL125 7491.1 Av. BDL125 7491.5P 0.37 0.05 BDL125 7491.5 Av. 1.27 1.16 BDL125 7492.5 P 0.43 0.54 0.560.40 0.13 0.56 BDL125 7492.5 Av. 1.72 1.62 1.53 1.12 1.16 1.53 BDL1257494.1 P 0.54 0.03 0.59 0.20 0.59 BDL125 7494.1 Av. 1.65 1.65 1.21 1.101.21 BDL125 7495.5 P 0.48 0.33 0.17 0.43 0.37 0.47 0.43 BDL125 7495.5Av. 1.41 1.28 1.75 1.50 1.14 1.24 1.50 BDL128 7711.3 P 0.55 0.79 0.590.82 0.85 0.53 0.82 BDL128 7711.3 Av. 1.41 1.26 1.73 1.12 1.12 1.13 1.12BDL128 8361.5 P 0.50 0.64 0.53 0.43 0.36 BDL128 8361.5 Av. 4.09 1.613.76 1.14 1.30 BDL128 8362.2 P 0.72 BDL128 8362.2 Av. 1.15 BDL128 8363.2P 0.48 0.60 BDL128 8363.2 Av. 2.28 1.46 BDL128 8365.2 P 0.48 0.49 0.480.46 BDL128 8365.2 Av. 7.01 1.50 5.56 3.18 BDL129 7691.4 P 0.26 0.70BDL129 7691.4 Av. 1.41 1.25 BDL129 7691.6 P 0.25 0.04 0.34 0.16 0.02BDL129 7691.6 Av. 1.88 1.34 1.38 1.34 1.28 BDL129 7692.2 P 0.50 0.090.51 0.51 BDL129 7692.2 Av. 11.03 1.77 6.06 7.47 BDL129 7692.6 P 0.060.68 BDL129 7692.6 Av. 1.31 1.19 BDL129 7693.1 P 0.50 0.50 0.49 BDL1297693.1 Av. 12.70 6.16 3.58 BDL130 7663.1 P 0.35 0.08 0.66 BDL130 7663.1Av. 1.26 1.14 1.15 BDL130 7663.3 P 0.43 0.33 0.27 0.01 0.19 0.03 0.05BDL130 7663.3 Av. 6.12 1.67 1.82 1.41 1.13 1.21 1.43 BDL130 7663.6 P0.11 BDL130 7663.6 Av. 1.24 BDL130 7664.5 P BDL130 7664.5 Av. BDL1317461.2 P 0.38 0.00 0.00 BDL131 7461.2 Av. 1.16 1.30 1.16 BDL131 7461.4 PBDL131 7461.4 Av. BDL131 7462.2 P BDL131 7462.2 Av. BDL131 7463.4 P 0.410.16 0.01 BDL131 7463.4 Av. 1.26 1.21 1.24 BDL131 7464.5 P 0.57 0.340.06 0.26 BDL132 7464.5 Av. 1.13 1.57 1.14 1.25 BDL132 7471.1 P 0.000.43 0.05 0.05 BDL132 7471.1 Av. 1.79 1.10 1.74 1.74 BDL132 7471.4 P0.72 0.72 BDL132 7471.4 Av. 1.21 1.21 BDL132 7472.4 P 0.45 0.27 0.030.45 0.45 BDL132 7472.4 Av. 1.89 1.15 1.31 1.30 1.30 BDL132 7473.1 P0.47 0.46 0.50 BDL132 7473.1 Av. 4.65 4.79 69.02 BDL132 7474.4 P 0.930.50 0.68 0.51 0.49 BDL132 7474.4 Av. 1.24 8.05 1.24 5.70 10.13 BDL1327471.1 P 0.50 0.23 BDL132 7471.1 Av. 1.28 1.24 BDL132 7471.4 P 0.45 0.100.10 BDL132 7471.4 Av. 1.84 1.16 1.16 BDL132 7472.4 P 0.49 0.10 BDL1327472.4 Av. 1.87 1.09 BDL132 7473.1 P 0.63 0.29 0.61 0.28 0.03 BDL1327473.1 Av. 1.20 1.38 1.36 1.23 1.21 BDL132 7475.4 P 0.37 0.70 0.51BDL132 7475.4 Av. 1.75 1.11 1.16 BDL133 8161.1 P 0.02 0.56 0.74 BDL1338161.1 Av. 2.12 1.17 1.43 BDL133 8161.2 P 0.34 BDL133 8161.2 Av. 1.16BDL133 8161.3 P 0.32 0.48 0.66 0.73 0.63 0.60 0.30 0.73 BDL133 8161.3Av. 1.12 15.21 1.24 1.31 1.13 1.76 1.22 1.31 BDL133 8161.4 P 0.16 0.360.12 0.24 0.00 BDL133 8161.4 Av. 1.13 1.48 1.24 1.13 1.43 BDL133 8162.1P 0.07 BDL133 8162.1 Av. 1.39 BDL133 8162.3 P 0.19 BDL133 8162.3 Av.1.13 BDL133 8162.5 P 0.02 BDL133 8162.5 Av. 1.20 BDL133 8163.2 P 0.51BDL133 8163.2 Av. 1.38 BDL134 7671.2 P 0.40 0.02 0.26 0.57 0.11 BDL1347671.2 Av. 3.72 1.79 1.15 3.46 1.22 BDL134 7672.1 P 0.26 0.44 0.08 0.490.00 BDL134 7672.1 Av. 4.40 1.24 1.26 1.14 1.29 BDL134 7673.1 P 0.460.16 0.07 0.07 0.01 BDL134 7673.1 Av. 13.42 1.44 1.30 1.14 1.37 BDL1347673.2 P 0.02 0.16 0.15 0.32 BDL134 7673.2 Av. 1.63 1.28 1.18 1.12BDL135 7722.1 P 0.47 0.29 BDL135 7722.1 Av. 1.18 1.16 BDL135 7723.1 P0.56 0.62 0.03 0.57 0.54 0.62 BDL135 7723.1 Av. 1.14 2.29 1.18 3.83 1.222.29 BDL135 7723.3 P BDL135 7723.3 Av. BDL135 7723.8 P 0.74 0.25 0.56BDL135 7723.8 Av. 1.15 1.12 1.13 BDL135 7723.9 P 0.71 BDL135 7723.9 Av.1.14 BDL136 7751.4 P 0.05 0.13 BDL136 7751.4 Av. 1.51 1.12 BDL136 7751.5P 0.38 0.39 0.42 0.24 0.40 0.42 BDL136 7751.5 Av. 1.51 1.34 1.11 1.111.11 1.12 BDL136 7751.8 P 0.12 BDL136 7751.8 Av. 1.10 BDL136 7752.6 P0.40 0.16 BDL136 7752.6 Av. 1.16 1.16 BDL137 7701.2 P 0.50 0.52 0.750.13 0.73 0.41 0.41 0.75 BDL137 7701.2 Av. 14.76 1.24 1.41 1.25 1.421.11 1.14 1.41 BDL137 7701.5 P 0.53 0.64 0.05 BDL137 7701.5 Av. 1.501.25 1.14 BDL137 7701.6 P 0.76 0.55 BDL137 7701.6 Av. 1.13 1.11 BDL1377702.1 P 0.05 0.37 0.03 0.17 0.25 0.16 BDL137 7702.1 Av. 2.52 1.20 1.511.25 1.13 1.43 BDL137 7703.2 P 0.18 0.10 0.07 BDL137 7703.2 Av. 1.241.43 1.19 BDL137 7703.3 P 0.34 0.21 0.67 0.22 0.00 BDL137 7703.3 Av.1.27 1.35 1.11 1.15 1.29 BDL137 7703.7 P 0.15 0.02 0.10 BDL137 7703.7Av. 1.32 1.21 1.12 BDL139 8131.1 P BDL139 8131.1 Av. BDL139 8131.2 P0.25 0.01 0.63 0.56 0.05 0.26 BDL139 8131.2 Av. 1.19 1.83 1.16 1.13 1.231.16 BDL139 8132.7 P BDL139 8132.7 Av. BDL139 8133.2 P BDL139 8133.2 Av.BDL141 8141.2 P 0.15 BDL141 8141.2 Av. 1.16 BDL141 8142.2 P BDL1418142.2 Av. BDL142 8282.1 P 0.03 0.43 0.62 0.20 0.03 0.10 BDL142 8282.1Av. 1.16 1.25 1.25 1.14 1.20 1.12 BDL142 8283.1 P 0.41 0.33 0.52 0.110.59 0.16 BDL142 8283.1 Av. 1.23 8.56 1.27 1.16 2.47 1.28 BDL142 8283.2P 0.27 0.05 0.30 0.48 BDL142 8283.2 Av. 1.14 1.42 1.18 1.15 BDL1428284.1 P 0.83 0.57 0.41 0.38 BDL142 8284.1 Av. 1.11 1.14 1.16 1.32BDL142 8285.3 P 0.03 0.34 0.65 0.41 0.44 0.57 0.01 0.18 BDL142 8285.3Av. 1.17 2.67 1.20 1.22 1.16 2.90 1.31 1.21 BDL142 8285.5 P BDL1428285.5 Av. BDL143 8411.1 P 0.40 0.37 BDL143 8411.1 Av. 1.21 1.24 BDL1438411.5 P 0.54 0.54 BDL143 8411.5 Av. 1.13 1.10 BDL143 8412.2 P 0.43 0.010.03 0.14 BDL143 8412.2 Av. 1.32 1.54 1.22 1.28 BDL143 8412.4 P 0.010.47 0.56 0.03 BDL143 8412.4 Av. 2.08 1.18 1.17 1.30 BDL143 8413.3 P0.02 0.15 0.18 0.00 BDL143 8413.3 Av. 1.72 1.35 1.11 1.24 BDL143 8414.4P 0.36 0.79 BDL143 8414.4 Av. 1.18 1.15 BDL143 8414.5 P 0.16 BDL1438414.5 Av. 1.17 BDL144 8384.1 P 0.58 0.28 0.18 0.41 BDL144 8384.1 Av.1.33 1.47 1.13 1.18 BDL144 8384.5 P 0.08 0.14 BDL144 8384.5 Av. 1.451.20 BDL144 8385.1 P 0.00 0.76 0.06 0.40 0.05 0.23 BDL144 8385.1 Av.3.33 1.11 1.69 1.24 1.20 1.40 BDL145 8233.2 P 0.15 0.05 0.13 0.15 BDL1458233.2 Av. 1.48 1.51 1.23 1.14 BDL145 8233.3 P 0.39 0.02 0.58 0.56 0.050.00 BDL145 8233.3 Av. 14.58 1.62 1.15 1.12 1.28 1.28 BDL145 8235.1 P0.30 0.15 0.21 BDL145 8235.1 Av. 1.19 1.53 1.20 BDL145 8235.3 P 0.190.02 0.29 0.39 0.11 BDL145 8235.3 Av. 5.48 1.65 1.20 1.31 1.27 BDL1458235.4 P BDL145 8235.4 Av. BDL146 8241.1 P 0.17 BDL146 8241.1 Av. 1.32BDL146 8241.3 P 0.31 0.60 0.00 0.00 BDL146 8241.3 Av. 1.18 1.24 1.171.35 BDL146 8243.2 P 0.20 BDL146 8243.2 Av. 1.14 BDL146 8243.5 P 0.730.33 0.44 0.03 0.00 BDL146 8243.5 Av. 1.22 1.43 1.42 1.23 1.39 BDL1468244.4 P 0.43 0.19 0.58 0.08 0.67 0.43 0.58 BDL146 8244.4 Av. 1.15 1.262.83 1.16 1.68 1.21 2.83 BDL146 8244.7 P 0.00 0.61 0.23 0.15 0.07 BDL1468244.7 Av. 1.96 1.18 1.39 1.14 1.33 BDL146 8245.2 P 0.10 0.03 0.02BDL146 8245.2 Av. 1.34 1.18 1.50 BDL146 8245.5 P BDL146 8245.5 Av. BDL427771.1 P BDL42 7771.1 Av. BDL42 7772.1 P BDL42 7772.1 Av. BDL42 7772.7 PBDL42 7772.7 Av. BDL42 7774.1 P 0.07 0.58 0.00 0.06 0.24 0.12 BDL427774.1 Av. 5.75 1.14 1.59 1.11 1.21 1.19 BDL42 7774.2 P BDL42 7774.2 Av.BDL42 7774.4 P 0.47 0.01 BDL42 7774.4 Av. 4.18 1.81 BDL46 7833.3 P 0.020.32 0.45 0.19 BDL46 7833.3 Av. 1.37 1.44 1.68 1.36 BDL46 7833.4 P 0.56BDL46 7833.4 Av. 1.19 BDL46 7833.5 P 0.47 0.45 0.51 0.54 0.54 BDL467833.5 Av. 2.25 1.14 1.32 1.31 1.31 BDL46 7833.6 P 0.14 0.30 0.61 BDL467833.6 Av. 1.21 1.24 1.19 BDL46 7834.1 P 0.75 0.46 BDL46 7834.1 Av. 1.131.15 BDL46 7833.1 P 0.20 0.43 0.09 0.02 0.21 0.39 BDL46 7833.1 Av. 2.161.47 1.70 1.18 1.32 1.20 BDL46 7833.3 P 0.81 BDL46 7833.3 Av. 1.20 BDL467833.4 P 0.33 BDL46 7833.4 Av. 1.73 BDL46 7833.5 P 0.65 0.17 0.32 BDL467833.5 Av. 1.14 1.77 1.24 BDL46 7834.4 P 0.02 0.27 0.02 0.02 0.29 0.00BDL46 7834.4 Av. 1.22 1.96 2.26 1.29 1.13 1.33 BDL51 7291.1 P 0.19 0.780.00 0.31 0.81 0.49 0.04 0.31 BDL51 7291.1 Av. 3.97 1.19 1.77 1.82 1.211.32 1.23 1.82 BDL51 8021.1 P 0.02 0.93 0.50 0.13 0.93 BDL51 8021.1 Av.3.28 1.18 26.01 1.15 1.18 BDL51 8022.4 P 0.04 0.03 0.34 0.77 0.40 BDL518022.4 Av. 2.98 2.43 1.54 1.17 1.15 BDL51 8022.5 P 0.35 0.44 0.44 0.130.03 0.50 BDL51 8022.5 Av. 1.19 4.24 2.39 3.03 1.43 1.25 BDL51 8024.4 P0.39 0.00 0.28 0.66 0.53 0.30 0.55 0.28 0.66 BDL51 8024.4 Av. 3.38 1.691.63 1.32 1.30 1.15 1.30 1.26 1.32 BDL51 8024.7 P 0.33 0.37 0.41 BDL518024.7 Av. 1.29 1.39 1.21 BDL52 7861.1 P 0.72 0.57 0.61 0.61 BDL527861.1 Av. 1.25 1.24 1.18 1.18 BDL52 7861.5 P 0.11 0.68 0.01 BDL527861.5 Av. 1.31 1.14 1.27 BDL52 7863.2 P 0.82 BDL52 7863.2 Av. 1.21BDL52 7864.5 P 0.61 0.76 0.71 0.40 0.76 BDL52 7864.5 Av. 1.25 1.17 1.101.26 1.17 BDL54 7781.1 P 0.50 0.73 0.50 0.47 BDL54 7781.1 Av. 13.03 1.166.59 3.90 BDL54 7781.4 P 0.06 0.09 0.78 0.27 0.55 BDL54 7781.4 Av. 1.281.25 1.13 1.19 1.22 BDL54 7784.3 P 0.87 0.49 0.16 0.48 0.05 0.49 0.06BDL54 7784.3 Av. 1.49 9.56 1.68 5.77 1.15 14.83 1.26 BDL54 7784.5 P 0.440.47 0.07 BDL54 7784.5 Av. 1.49 1.19 1.27 BDL54 7785.4 P 0.16 0.06 0.360.00 BDL54 7785.4 Av. 1.52 1.40 1.22 1.30 BDL54 7781.1 P 0.55 0.19 0.080.23 0.10 BDL54 7781.1 Av. 1.50 1.33 3.05 1.13 1.27 BDL54 7781.4 P 0.040.25 0.01 0.52 0.00 BDL54 7781.4 Av. 1.87 1.36 1.85 1.18 1.37 BDL547784.3 P 0.49 0.47 0.43 0.02 0.03 BDL54 7784.3 Av. 1.54 1.37 1.81 1.141.30 BDL54 7785.4 P 0.40 0.67 BDL54 7785.4 Av. 1.57 1.13 BDL54 7785.8 P0.41 0.07 0.10 BDL54 7785.8 Av. 1.39 1.21 1.28 BDL56 7181.2 P 0.51 0.510.50 0.43 BDL56 7181.2 Av. 3.81 1.79 3.28 3.75 BDL56 8301.1 P BDL568301.1 Av. BDL56 8301.3 P 0.42 0.64 0.34 BDL56 8301.3 Av. 1.10 1.15 1.11BDL56 8304.1 P 0.40 BDL56 8304.1 Av. 1.12 BDL56 8305.1 P 0.19 0.52 0.760.71 0.76 BDL56 8305.1 Av. 1.17 1.26 1.11 1.13 1.11 BDL56 8301.1 P 0.640.70 0.70 0.65 0.41 0.40 BDL56 8301.1 Av. 1.21 1.29 1.11 1.33 1.15 1.19BDL56 8301.2 P 0.44 0.13 0.01 0.01 0.12 BDL56 8301.2 Av. 2.00 1.67 1.171.30 1.32 BDL56 8301.3 P 0.23 0.23 0.63 0.61 0.38 0.61 BDL56 8301.3 Av.1.11 1.70 1.46 1.18 1.36 1.18 BDL56 8303.1 P 0.53 BDL56 8303.1 Av. 1.39BDL56 8303.2 P 0.29 0.39 0.16 0.16 BDL56 8303.2 Av. 1.32 1.78 1.12 1.12BDL56 7792.1 P BDL59 7792.1 Av. BDL59 7792.2 P 0.70 0.20 BDL59 7792.2Av. 1.19 1.16 BDL59 7792.3 P 0.31 BDL59 7792.3 Av. 1.43 BDL59 7793.3 P0.66 0.04 0.57 0.56 0.57 BDL59 7793.3 Av. 1.48 1.43 1.40 1.11 1.40 BDL597794.1 P 0.13 0.01 0.36 BDL59 7794.1 Av. 7.90 6.46 4.80 BDL60 8011.4 PBDL60 8011.4 Av. BDL60 8011.7 P 0.49 0.00 0.48 0.47 0.06 BDL60 8011.7Av. 10.85 1.51 5.59 2.44 1.28 BDL60 8013.4 P 0.97 0.39 0.50 0.38 0.490.35 BDL60 8013.4 Av. 1.10 1.66 27.18 1.13 16.70 1.20 BDL60 8013.6 P0.35 0.74 0.62 0.14 0.25 BDL60 8013.6 Av. 2.43 1.34 1.18 1.10 1.12 BDL608014.5 P 0.48 0.10 0.61 0.72 0.37 0.61 BDL60 8014.5 Av. 3.21 1.73 1.431.20 1.12 1.43 BDL60 8013.6 P 0.78 0.59 0.02 0.24 0.35 BDL60 8013.6 Av.1.15 1.37 1.12 1.22 1.15 BDL60 8014.2 P 0.02 0.01 0.02 0.01 BDL60 8014.2Av. 2.03 2.05 1.35 1.19 BDL60 8014.7 P 0.40 0.17 0.72 0.07 0.01 0.04BDL60 8014.7 Av. 1.23 1.32 1.20 1.22 1.33 1.17 BDL60 8014.8 P 0.72 0.010.46 0.14 0.61 0.29 0.46 BDL60 8014.8 Av. 1.53 1.95 1.30 1.10 1.19 1.181.30 BDL65 7824.1 P 0.73 0.69 0.27 BDL65 7824.1 Av. 1.40 1.11 1.13 BDL657825.2 P 0.20 0.56 0.03 BDL65 7825.2 Av. 1.25 1.33 1.33 BDL65 8473.2 P0.10 0.37 0.00 0.01 0.01 0.45 0.50 0.05 BDL65 8473.2 Av. 4.55 1.17 1.691.27 1.22 3.42 1.42 1.33 BDL65 8474.1 P 0.58 BDL65 8474.1 Av. 1.11 BDL657901.5 P 0.42 0.46 0.00 0.52 0.38 0.52 BDL67 7901.5 Av. 1.92 1.10 1.701.27 1.13 1.27 BDL67 7902.3 P 0.30 0.48 0.48 0.50 0.50 BDL67 7902.3 Av.1.22 8.10 5.44 20.18 1.19 BDL67 7902.7 P 0.49 0.12 0.50 0.08 0.50 BDL677902.7 Av. 8.01 1.43 6.31 1.13 6.95 BDL67 7903.3 P 0.52 0.33 BDL677903.3 Av. 1.23 1.21 BDL67 7903.5 P 0.26 0.00 0.35 0.47 0.00 0.15 0.47BDL67 7903.5 Av. 1.10 3.19 1.61 1.29 1.26 1.39 1.29 BDL68 7761.3 P 0.260.49 0.50 BDL68 7761.3 Av. 1.25 12.65 24.18 BDL68 7761.8 P 0.48 0.500.01 0.19 0.08 0.36 0.08 BDL68 7761.8 Av. 10.41 1.23 1.49 1.67 1.18 1.421.41 BDL68 7761.9 P 0.25 0.49 0.44 0.59 0.13 0.00 0.49 BDL68 7761.9 Av.1.73 2.09 1.22 1.23 1.69 1.45 2.09 BDL68 7763.2 P 0.40 0.03 0.51 0.020.75 0.05 0.20 0.51 BDL68 7763.2 Av. 3.14 1.55 1.54 1.18 1.20 1.46 1.211.54 BDL68 7764.1 P 0.55 0.50 0.33 0.49 0.03 0.47 0.01 BDL68 7764.1 Av.10.21 13.08 1.50 7.93 1.16 5.30 1.27 BDL78 7911.11 P 0.13 0.52 0.35BDL78 7911.11 Av. 1.12 1.17 1.23 BDL78 7911.8 P 0.51 0.64 0.07 0.22BDL78 7911.8 Av. 1.15 1.26 1.07 1.11 BDL78 7911.9 P 0.22 0.00 0.06 0.380.39 BDL78 7911.9 Av. 7.52 1.82 1.19 1.17 1.16 BDL78 7912.6 P 0.58 0.140.20 0.24 BDL78 7912.6 Av. 1.10 1.31 1.19 1.22 BDL78 7913.11 P 0.20 0.210.14 0.29 0.53 0.36 BDL78 7913.11 Av. 1.18 1.99 1.13 1.11 1.23 1.43BDL78 7913.3 P BDL78 7913.3 Av. BDL78 7913.6 P 0.42 0.04 0.09 0.61 0.01BDL78 7913.6 Av. 11.13 1.45 1.21 1.23 1.22 BDL78 7913.8 P 0.11 0.07 0.04BDL78 7913.8 Av. 1.33 1.16 1.15 BDL78 7913.9 P 0.48 0.63 0.24 0.54 0.01BDL78 7913.9 Av. 1.85 1.26 1.16 5.72 1.22 BDL82 7801.1 P 0.31 0.01 0.230.05 0.37 0.27 0.23 BDL82 7801.1 Av. 3.94 1.88 1.44 1.14 1.45 1.41 1.44BDL82 7801.3 P 0.39 0.47 0.51 0.47 BDL82 7801.3 Av. 13.27 1.36 1.21 1.36BDL82 7802.2 P 0.41 0.46 0.52 0.07 0.45 0.52 BDL82 7802.2 Av. 3.78 1.201.54 1.34 1.17 1.54 BDL82 7802.3 P 0.18 0.02 0.45 0.32 0.12 0.06 BDL827802.3 Av. 2.14 1.41 1.40 1.14 1.16 1.24 BDL82 7803.9 P 0.54 BDL827803.9 Av. 1.18 BDL89 7812.2 P 0.67 0.26 0.42 BDL89 7812.2 Av. 1.12 1.171.13 BDL89 7812.5 P 0.51 0.51 0.18 0.50 BDL89 7812.5 Av. 12.24 5.84 1.1816.66 BDL89 7814.1 P 0.23 0.39 0.58 0.06 BDL89 7814.1 Av. 1.16 1.14 1.161.16 BDL89 7814.4 P 0.47 0.42 0.46 0.05 0.48 0.43 BDL89 7814.4 Av. 9.402.02 5.59 1.17 6.76 1.21 BDL89 7814.5 P 0.47 0.26 0.46 0.52 0.20 0.150.46 BDL89 7814.5 Av. 7.62 1.57 1.98 1.65 1.26 1.14 1.98 Gene Ev. Par.46 47 48 49 50 51 52 53 54 55 BDL95 7841.5 P 0.55 0.03 0.01 BDL95 7841.5Av. 1.11 1.14 1.09 BDL95 7842.12 P 0.12 0.12 0.32 0.05 BDL95 7842.12 Av.1.24 1.11 1.28 1.04 BDL95 7842.2 P 0.07 0.12 0.19 0.20 0.00 BDL95 7842.2Av. 1.07 1.11 1.33 1.14 1.12 BDL95 7842.8 P 0.01 0.20 0.01 BDL95 7842.8Av. 1.21 1.31 1.09 BDL95 7843.4 P 0.49 0.01 BDL95 7843.4 Av. 5.89 1.24BDL100 7871.2 P 0.37 0.09 0.34 0.39 BDL100 7871.2 Av. 1.14 1.12 1.101.16 BDL100 7872.2 P 0.26 0.27 0.12 0.00 BDL100 7872.2 Av. 1.20 1.171.11 1.49 BDL100 7872.3 P 0.56 0.32 BDL100 7872.3 Av. 1.11 1.27 BDL1007873.3 P 0.42 0.03 0.32 0.12 BDL100 7873.3 Av. 1.18 1.16 1.20 1.11BDL100 7873.4 P 0.43 0.06 BDL100 7873.4 Av. 2.58 1.41 BDL106 7881.1 P0.03 BDL106 7881.1 Av. 1.14 BDL106 7881.4 P 0.23 0.16 BDL106 7881.4 Av.1.42 1.23 BDL106 7882.6 P 0.04 0.30 BDL106 7882.6 Av. 1.15 1.10 BDL1067884.1 P 0.50 0.47 0.12 BDL106 7884.1 Av. 5.79 1.22 1.11 BDL106 7884.9 P0.47 0.03 0.00 0.00 BDL106 7884.9 Av. 1.13 1.26 1.09 1.09 BDL106 7881.1P 0.23 BDL106 7881.1 Av. 1.16 BDL106 7881.2 P 0.38 0.37 BDL106 7881.2Av. 1.14 1.16 BDL106 7882.2 P BDL106 7882.2 Av. BDL106 7882.4 P 0.24BDL106 7882.4 Av. 1.12 BDL106 7882.5 P 0.43 0.35 0.50 BDL106 7882.5 Av.1.21 1.14 1.13 BDL108 8122.2 P 0.49 0.39 0.06 0.26 0.08 BDL108 8122.2Av. 4.63 1.16 1.27 1.15 1.09 BDL108 8122.3 P 0.31 0.05 0.25 0.00 BDL1088122.3 Av. 1.13 1.20 1.17 1.11 BDL108 8123.1 P 0.15 BDL108 8123.1 Av.1.13 BDL108 8123.2 P 0.52 0.50 0.00 0.05 0.53 BDL108 8123.2 Av. 6.481.30 1.35 1.15 1.21 BDL108 8123.5 P 0.13 0.09 0.05 BDL108 8123.5 Av.1.11 1.30 1.15 BDL108 8121.1 P BDL108 8121.1 Av. BDL108 8121.3 P BDL1088121.3 Av. BDL108 8121.4 P BDL108 8121.4 Av. BDL108 8122.7 P BDL1088122.7 Av. BDL108 8123.7 P BDL108 8123.7 Av. BDL110 8092.1 P 0.18 0.02BDL110 8092.1 Av. 1.12 1.15 BDL110 8092.2 P 0.01 0.00 0.03 BDL110 8092.2Av. 1.20 1.30 1.21 BDL110 8092.5 P 0.04 0.05 0.00 BDL110 8092.5 Av. 1.091.14 1.12 BDL110 8095.2 P 0.20 0.01 BDL110 8095.2 Av. 1.16 1.10 BDL1118102.7 P 0.04 0.15 0.00 0.37 BDL111 8102.7 Av. 1.24 1.26 1.15 1.28BDL111 8103.1 P 0.20 0.00 0.00 BDL111 8103.1 Av. 1.54 1.20 1.16 BDL1118103.2 P 0.03 0.30 0.00 BDL111 8103.2 Av. 1.34 1.14 1.37 BDL111 8103.4 P0.00 0.12 0.12 0.00 BDL111 8103.4 Av. 1.19 1.11 1.15 1.08 BDL111 8103.5P 0.64 0.27 0.00 0.01 BDL111 8103.5 Av. 1.12 1.13 1.16 1.09 BDL1118102.7 P 0.44 BDL111 8102.7 Av. 1.12 BDL111 8103.1 P 0.24 0.18 BDL1118103.1 Av. 1.12 1.17 BDL111 8103.2 P BDL111 8103.2 Av. BDL111 8103.4 PBDL111 8103.4 Av. BDL111 8103.5 P 0.10 BDL111 8103.5 Av. 1.11 BDL1127502.1 P 0.07 0.31 0.07 BDL112 7502.1 Av. 1.30 1.13 1.22 BDL112 7502.14P 0.49 0.56 0.12 BDL112 7502.14 Av. 4.98 1.11 1.16 BDL112 7502.4 P 0.480.00 0.00 0.03 BDL112 7502.4 Av. 1.31 1.16 1.09 1.07 BDL112 7502.7 P0.45 0.03 BDL112 7502.7 Av. 1.21 1.08 BDL112 7502.9 P 0.48 0.00 0.08BDL112 7502.9 Av. 1.29 1.21 1.10 BDL112 7502.1 P 0.03 BDL112 7502.1 Av.1.11 BDL112 7502.4 P 0.02 0.08 BDL112 7502.4 Av. 1.13 1.10 BDL112 7502.7P 0.00 0.34 0.05 BDL112 7502.7 Av. 1.23 1.12 1.23 BDL112 7502.8 P 0.01BDL112 7502.8 Av. 1.15 BDL112 7502.9 P 0.11 0.28 0.09 BDL112 7502.9 Av.1.12 1.25 1.41 BDL113 7683.4 P 0.01 0.39 0.23 BDL113 7683.4 Av. 1.231.28 1.14 BDL113 7683.6 P 0.03 0.39 0.26 BDL113 7683.6 Av. 1.47 1.291.12 BDL113 7684.3 P 0.34 0.17 BDL113 7684.3 Av. 1.30 1.14 BDL113 7684.6P 0.51 0.00 0.01 BDL113 7684.6 Av. 1.12 1.17 1.09 BDL113 7684.7 P 0.220.00 BDL113 7684.7 Av. 1.19 1.15 BDL113 7683.1 P 0.54 BDL113 7683.1 Av.1.15 BDL113 7683.11 P BDL113 7683.11 Av. BDL113 7683.4 P 0.69 BDL1137683.4 Av. 1.12 BDL113 7684.1 P BDL113 7684.1 Av. BDL113 7684.5 P BDL1137684.5 Av. BDL114 7741.3 P 0.50 0.10 0.26 0.10 BDL114 7741.3 Av. 10.331.14 1.27 1.15 BDL114 7741.6 P 0.49 0.40 0.72 BDL114 7741.6 Av. 11.281.23 1.12 BDL114 7742.1 P 0.39 0.16 BDL114 7742.1 Av. 1.22 1.13 BDL1147742.3 P 0.03 0.20 0.15 BDL114 7742.3 Av. 1.69 1.41 1.16 BDL114 7742.5 P0.45 0.17 0.06 0.07 BDL114 7742.5 Av. 1.58 1.14 1.44 1.21 BDL115 8152.3P 0.11 0.24 0.03 0.08 BDL115 8152.3 Av. 1.26 1.14 1.25 1.13 BDL1158152.4 P 0.07 0.15 BDL115 8152.4 Av. 1.12 1.13 BDL115 8154.1 P 0.40 0.050.00 0.31 BDL115 8154.1 Av. 1.32 1.31 1.14 1.36 BDL115 8155.2 P 0.500.00 0.12 0.50 BDL115 8155.2 Av. 6.80 1.24 1.11 1.13 BDL115 8155.4 P0.51 0.12 0.12 BDL115 8155.4 Av. 3.59 1.27 1.11 BDL115 8152.3 P 0.01BDL115 8152.3 Av. 1.27 BDL115 8152.4 P 0.25 0.08 BDL115 8152.4 Av. 1.201.19 BDL115 8154.1 P 0.01 0.17 BDL115 8154.1 Av. 1.16 1.43 BDL115 8155.2P BDL115 8155.2 Av. BDL115 8155.4 P BDL115 8155.4 Av. BDL116 7481.2 P0.12 0.02 0.00 BDL116 7481.2 Av. 1.21 1.32 1.17 BDL116 7481.7 P 0.440.00 0.00 0.27 BDL116 7481.7 Av. 1.13 1.24 1.13 1.10 BDL116 7481.8 P0.50 0.56 0.36 0.04 0.60 BDL116 7481.8 Av. 5.33 1.12 1.11 1.22 1.33BDL116 7482.2 P 0.02 0.00 0.00 BDL116 7482.2 Av. 1.18 1.20 1.04 BDL1167485.1 P 0.46 0.08 0.12 0.24 BDL116 7485.1 Av. 1.27 1.20 1.27 1.24BDL119 7732.2 P 0.09 0.02 0.00 0.12 BDL119 7732.2 Av. 1.16 1.10 1.111.13 BDL119 7733.2 P 0.06 BDL119 7733.2 Av. 1.21 BDL119 7734.1 P 0.520.21 0.02 0.01 BDL119 7734.1 Av. 1.12 1.13 1.11 1.10 BDL119 7734.5 P0.14 BDL119 7734.5 Av. 1.13 BDL119 7734.7 P BDL119 7734.7 Av. BDL1207891.3 P 0.02 0.00 0.12 0.51 BDL120 7891.3 Av. 1.19 1.34 1.11 1.22BDL120 7892.4 P 0.03 0.23 0.37 BDL120 7892.4 Av. 1.28 1.15 1.11 BDL1207892.6 P 0.15 0.00 0.07 BDL120 7892.6 Av. 1.13 1.34 1.11 BDL120 7893.2 P0.19 0.14 0.07 0.21 0.06 BDL120 7893.2 Av. 11.26 1.13 1.29 1.11 1.20BDL120 7893.5 P 0.03 0.25 BDL120 7893.5 Av. 1.08 1.17 BDL122 7513.1 P0.29 0.13 BDL122 7513.1 Av. 1.15 1.13 BDL122 7513.1 P 0.18 BDL122 7513.1Av. 1.22 BDL122 7513.14 P 0.31 0.14 0.01 0.10 0.00 BDL122 7513.14 Av.1.18 1.25 1.37 1.13 1.08 BDL122 7513.9 P 0.48 0.21 0.01 BDL122 7513.9Av. 5.25 1.14 1.10 BDL122 7514.3 P 0.53 0.51 BDL122 7514.3 Av. 4.19 1.20BDL122 7513.1 P 0.33 0.47 BDL122 7513.1 Av. 1.16 1.14 BDL122 7513.14 PBDL122 7513.14 Av. BDL122 7513.9 P BDL122 7513.9 Av. BDL122 7514.3 PBDL122 7514.3 Av. BDL123 8082.1 P 0.00 0.49 BDL123 8082.1 Av. 1.30 1.21BDL123 8082.3 P 0.03 BDL123 8082.3 Av. 1.24 BDL123 8082.6 P 0.68 0.13BDL123 8082.6 Av. 1.17 1.21 BDL123 8083.2 P 0.50 0.28 0.26 0.07 BDL1238083.2 Av. 9.06 1.38 1.15 1.32 BDL123 8083.3 P 0.07 BDL123 8083.3 Av.1.03 BDL124 8482.1 P 0.01 0.13 0.22 BDL124 8482.1 Av. 1.12 1.30 1.29BDL125 7491.1 P 0.04 BDL125 7491.1 Av. 1.23 BDL125 7491.5 P 0.05 BDL1257491.5 Av. 1.16 BDL125 7492.5 P 0.02 0.00 0.40 0.06 BDL125 7492.5 Av.1.21 1.13 1.23 1.04 BDL125 7494.1 P 0.20 0.00 0.00 BDL125 7494.1 Av.1.10 1.17 1.09 BDL125 7495.5 P 0.06 0.00 0.28 BDL125 7495.5 Av. 1.141.09 1.20 BDL128 7711.3 P 0.85 0.53 0.18 0.00 0.24 BDL128 7711.3 Av.1.12 1.13 1.29 1.15 1.20 BDL128 8361.5 P 0.53 0.43 0.26 0.30 0.05 BDL1288361.5 Av. 3.76 1.14 1.16 1.12 1.04 BDL128 8362.2 P 0.26 0.02 0.01 0.310.04 BDL128 8362.2 Av. 1.12 1.08 1.36 1.23 1.11 BDL128 8363.2 P 0.160.41 0.06 BDL128 8363.2 Av. 1.14 1.24 1.11 BDL128 8365.2 P 0.48 0.060.00 0.01 BDL128 8365.2 Av. 5.56 1.43 1.17 1.42 BDL129 7691.4 P 0.050.00 0.04 BDL129 7691.4 Av. 1.17 1.15 1.08 BDL129 7691.6 P 0.16 0.330.12 BDL129 7691.6 Av. 1.34 1.26 1.16 BDL129 7692.2 P 0.51 0.42 BDL1297692.2 Av. 6.06 1.15 BDL129 7692.6 P BDL129 7692.6 Av. BDL129 7693.1 P0.50 0.00 0.17 BDL129 7693.1 Av. 6.16 1.21 1.14 BDL130 7663.1 P 0.080.33 0.03 BDL130 7663.1 Av. 1.14 1.16 1.13 BDL130 7663.3 P 0.01 0.190.03 0.13 BDL130 7663.3 Av. 1.41 1.13 1.35 1.28 BDL130 7663.6 P BDL1307663.6 Av. BDL130 7664.5 P BDL130 7664.5 Av. BDL131 7461.2 P 0.00 0.17BDL131 7461.2 Av. 1.30 1.25 BDL131 7461.4 P 0.16 BDL131 7461.4 Av. 1.17BDL131 7462.2 P BDL131 7462.2 Av. BDL131 7463.4 P 0.16 0.00 BDL1317463.4 Av. 1.21 1.09 BDL131 7464.5 P 0.06 0.00 0.03 0.11 BDL132 7464.5Av. 1.14 1.27 1.15 1.16 BDL132 7471.1 P BDL132 7471.1 Av. BDL132 7471.4P 0.60 BDL132 7471.4 Av. 1.27 BDL132 7472.4 P 0.02 BDL132 7472.4 Av.1.17 BDL132 7473.1 P 0.46 BDL132 7473.1 Av. 4.79 BDL132 7474.4 P 0.510.06 0.04 BDL132 7474.4 Av. 5.70 1.28 1.15 BDL132 7471.1 P BDL132 7471.1Av. BDL132 7471.4 P 0.41 BDL132 7471.4 Av. 1.25 BDL132 7472.4 P 0.100.57 0.81 BDL132 7472.4 Av. 1.09 1.34 1.14 BDL132 7473.1 P 0.28 0.21BDL132 7473.1 Av. 1.23 1.11 BDL132 7475.4 P BDL132 7475.4 Av. BDL1338161.1 P BDL133 8161.1 Av. BDL133 8161.2 P BDL133 8161.2 Av. BDL1338161.3 P 0.63 0.32 0.02 0.15 BDL133 8161.3 Av. 1.13 1.11 1.21 1.16BDL133 8161.4 P 0.24 0.05 0.04 0.43 BDL133 8161.4 Av. 1.13 1.08 1.121.17 BDL133 8162.1 P 0.42 0.01 BDL133 8162.1 Av. 1.10 1.13 BDL133 8162.3P 0.03 0.04 0.58 BDL133 8162.3 Av. 1.09 1.07 1.34 BDL133 8162.5 P 0.020.10 0.06 BDL133 8162.5 Av. 1.20 1.16 1.25 BDL133 8163.2 P 0.51 0.27BDL133 8163.2 Av. 1.13 1.27 BDL134 7671.2 P 0.26 0.11 0.01 BDL134 7671.2Av. 1.15 1.21 1.17 BDL134 7672.1 P 0.08 0.05 0.05 0.50 0.00 BDL1347672.1 Av. 1.26 1.08 1.10 1.12 1.25 BDL134 7673.1 P 0.07 0.02 0.02BDL134 7673.1 Av. 1.14 1.23 1.13 BDL134 7673.2 P 0.15 0.00 0.00 BDL1347673.2 Av. 1.18 1.16 1.10 BDL135 7722.1 P 0.29 BDL135 7722.1 Av. 1.16BDL135 7723.1 P 0.03 BDL135 7723.1 Av. 1.18 BDL135 7723.3 P 0.21 0.40BDL135 7723.3 Av. 1.22 1.15 BDL135 7723.8 P 0.25 0.01 BDL135 7723.8 Av.1.12 1.10 BDL135 7723.9 P 0.55 0.47 0.09 BDL135 7723.9 Av. 1.20 1.151.03 BDL136 7751.4 P 0.13 0.01 BDL136 7751.4 Av. 1.12 1.18 BDL136 7751.5P 0.24 0.02 0.01 0.33 BDL136 7751.5 Av. 1.11 1.10 1.08 1.10 BDL1367751.8 P 0.00 BDL136 7751.8 Av. 1.24 BDL136 7752.6 P 0.06 0.21 BDL1367752.6 Av. 1.20 1.11 BDL137 7701.2 P 0.13 0.27 BDL137 7701.2 Av. 1.251.10 BDL137 7701.5 P 0.01 0.10 0.62 BDL137 7701.5 Av. 1.22 1.15 1.13BDL137 7701.6 P 0.55 0.77 BDL137 7701.6 Av. 1.11 1.11 BDL137 7702.1 P0.17 0.25 0.07 0.15 BDL137 7702.1 Av. 1.25 1.13 1.24 1.19 BDL137 7703.2P 0.37 BDL137 7703.2 Av. 1.15 BDL137 7703.3 P 0.67 0.22 0.00 0.14 0.48BDL137 7703.3 Av. 1.11 1.15 1.27 1.16 1.17 BDL137 7703.7 P 0.02 0.060.18 BDL137 7703.7 Av. 1.21 1.23 1.13 BDL139 8131.1 P BDL139 8131.1 Av.BDL139 8131.2 P 0.56 0.08 0.02 0.10 BDL139 8131.2 Av. 1.13 1.15 1.151.19 BDL139 8132.7 P BDL139 8132.7 Av. BDL139 8133.2 P BDL139 8133.2 Av.BDL141 8141.2 P 0.15 0.05 BDL141 8141.2 Av. 1.11 1.12 BDL141 8142.2 PBDL141 8142.2 Av. BDL142 8282.1 P 0.20 0.01 0.00 0.68 BDL142 8282.1 Av.1.14 1.24 1.19 1.11 BDL142 8283.1 P 0.11 BDL142 8283.1 Av. 1.16 BDL1428283.2 P 0.30 0.32 0.43 BDL142 8283.2 Av. 1.18 1.12 1.20 BDL142 8284.1 P0.41 BDL142 8284.1 Av. 1.16 BDL142 8285.3 P 0.41 0.44 0.00 0.00 0.63BDL142 8285.3 Av. 1.22 1.16 1.27 1.16 1.12 BDL142 8285.5 P 0.49 BDL1428285.5 Av. 1.13 BDL143 8411.1 P BDL143 8411.1 Av. BDL143 8411.5 P 0.540.03 0.38 0.24 BDL143 8411.5 Av. 1.13 1.08 1.10 1.21 BDL143 8412.2 P0.03 0.03 0.00 0.05 BDL143 8412.2 Av. 1.22 1.32 1.24 1.03 BDL143 8412.4P 0.47 0.00 0.00 BDL143 8412.4 Av. 1.18 1.37 1.29 BDL143 8413.3 P 0.180.24 0.05 BDL143 8413.3 Av. 1.11 1.18 1.16 BDL143 8414.4 P 0.16 0.00BDL143 8414.4 Av. 1.11 1.18 BDL143 8414.5 P 0.11 0.01 BDL143 8414.5 Av.1.23 1.19 BDL144 8384.1 P 0.18 0.03 0.00 0.03 BDL144 8384.1 Av. 1.131.29 1.21 1.03 BDL144 8384.5 P 0.14 0.00 0.01 BDL144 8384.5 Av. 1.201.33 1.32 BDL144 8385.1 P 0.40 0.05 0.12 0.04 BDL144 8385.1 Av. 1.241.20 1.44 1.33 BDL145 8233.2 P 0.13 BDL145 8233.2 Av. 1.23 BDL145 8233.3P 0.58 0.56 0.11 0.10 BDL145 8233.3 Av. 1.15 1.12 1.27 1.21 BDL1458235.1 P 0.05 0.17 0.19 BDL145 8235.1 Av. 1.08 1.12 1.10 BDL145 8235.3 P0.29 0.02 0.03 BDL145 8235.3 Av. 1.20 1.21 1.15 BDL145 8235.4 P BDL1458235.4 Av. BDL146 8241.1 P BDL146 8241.1 Av. BDL146 8241.3 P 0.00 0.000.00 0.47 0.02 BDL146 8241.3 Av. 1.17 1.16 1.15 1.11 1.15 BDL146 8243.2P 0.20 0.00 0.02 BDL146 8243.2 Av. 1.14 1.20 1.15 BDL146 8243.5 P 0.030.10 0.01 BDL146 8243.5 Av. 1.23 1.19 1.10 BDL146 8244.4 P 0.08 0.17BDL146 8244.4 Av. 1.16 1.17 BDL146 8244.7 P 0.15 0.04 0.15 BDL146 8244.7Av. 1.14 1.18 1.19 BDL146 8245.2 P 0.03 0.38 BDL146 8245.2 Av. 1.18 1.35BDL146 8245.5 P 0.20 0.22 0.06 BDL146 8245.5 Av. 1.24 1.21 1.44 BDL427771.1 P BDL42 7771.1 Av. BDL42 7772.1 P BDL42 7772.1 Av. BDL42 7772.7 PBDL42 7772.7 Av. BDL42 7774.1 P 0.06 0.00 0.01 BDL42 7774.1 Av. 1.111.31 1.27 BDL42 7774.2 P BDL42 7774.2 Av. BDL42 7774.4 P 0.06 0.21 BDL427774.4 Av. 1.36 1.21 BDL46 7833.3 P 0.19 0.16 0.00 0.54 BDL46 7833.3 Av.1.36 1.30 1.15 1.15 BDL46 7833.4 P 0.00 BDL46 7833.4 Av. 1.40 BDL467833.5 P 0.04 0.07 0.19 BDL46 7833.5 Av. 1.14 1.03 1.15 BDL46 7833.6 P0.61 0.63 0.22 BDL46 7833.6 Av. 1.19 1.16 1.52 BDL46 7834.1 P 0.33 0.000.01 BDL46 7834.1 Av. 1.15 1.09 1.07 BDL46 7833.1 P 0.02 0.12 0.02 BDL467833.1 Av. 1.18 1.12 1.14 BDL46 7833.3 P BDL46 7833.3 Av. BDL46 7833.4 P0.73 0.75 BDL46 7833.4 Av. 1.25 1.25 BDL46 7833.5 P BDL46 7833.5 Av.BDL46 7834.4 P 0.02 BDL46 7834.4 Av. 1.29 BDL51 7291.1 P 0.04 0.01 0.00BDL51 7291.1 Av. 1.22 1.09 1.13 BDL51 8021.1 P 0.50 0.13 0.10 0.11 BDL518021.1 Av. 26.01 1.15 1.69 1.24 BDL51 8022.4 P 0.34 0.18 0.00 BDL518022.4 Av. 1.54 1.41 1.19 BDL51 8022.5 P 0.03 0.11 0.01 0.07 BDL518022.5 Av. 1.43 1.26 1.14 1.05 BDL51 8024.4 P 0.53 0.30 0.49 BDL518024.4 Av. 1.30 1.15 1.14 BDL51 8024.7 P 0.41 0.35 BDL51 8024.7 Av. 1.211.24 BDL52 7861.1 P 0.30 BDL52 7861.1 Av. 1.38 BDL52 7861.5 P 0.01 0.360.10 BDL52 7861.5 Av. 1.27 1.17 1.12 BDL52 7863.2 P 0.07 0.14 0.02 BDL527863.2 Av. 1.03 1.11 1.09 BDL52 7864.5 P 0.71 0.39 BDL52 7864.5 Av. 1.101.35 BDL54 7781.1 P 0.50 0.01 0.00 BDL54 7781.1 Av. 6.59 1.12 1.09 BDL547781.4 P 0.27 0.00 0.50 BDL54 7781.4 Av. 1.19 1.09 1.31 BDL54 7784.3 P0.48 0.05 0.00 0.05 0.22 BDL54 7784.3 Av. 5.77 1.15 1.35 1.15 1.12 BDL547784.5 P 0.47 0.23 0.75 BDL54 7784.5 Av. 1.19 1.15 1.12 BDL54 7785.4 P0.36 0.25 0.15 0.16 BDL54 7785.4 Av. 1.22 1.16 1.12 1.20 BDL54 7781.1 P0.23 BDL54 7781.1 Av. 1.13 BDL54 7781.4 P 0.52 0.15 BDL54 7781.4 Av.1.18 1.19 BDL54 7784.3 P 0.02 BDL54 7784.3 Av. 1.14 BDL54 7785.4 P 0.340.26 BDL54 7785.4 Av. 1.18 1.20 BDL54 7785.8 P 0.32 BDL54 7785.8 Av.1.21 BDL56 7181.2 P 0.50 0.00 0.00 0.70 BDL56 7181.2 Av. 3.28 1.42 1.191.13 BDL56 8301.1 P 0.01 0.05 BDL56 8301.1 Av. 1.05 1.24 BDL56 8301.3 P0.64 BDL56 8301.3 Av. 1.15 BDL56 8304.1 P BDL56 8304.1 Av. BDL56 8305.1P 0.71 0.23 BDL56 8305.1 Av. 1.13 1.20 BDL56 8301.1 P 0.41 BDL56 8301.1Av. 1.15 BDL56 8301.2 P 0.01 0.07 BDL56 8301.2 Av. 1.17 1.10 BDL568301.3 P 0.25 BDL56 8301.3 Av. 1.11 BDL56 8303.1 P BDL56 8303.1 Av.BDL56 8303.2 P 0.57 BDL56 8303.2 Av. 1.11 BDL56 7792.1 P 0.02 BDL597792.1 Av. 1.10 BDL59 7792.2 P BDL59 7792.2 Av. BDL59 7792.3 P 0.04BDL59 7792.3 Av. 1.06 BDL59 7793.3 P 0.56 0.36 0.20 BDL59 7793.3 Av.1.11 1.24 1.14 BDL59 7794.1 P 0.01 0.02 0.00 BDL59 7794.1 Av. 6.46 1.131.09 BDL60 8011.4 P 0.29 0.03 0.12 BDL60 8011.4 Av. 1.13 1.24 1.15 BDL608011.7 P 0.48 0.15 0.04 0.38 0.04 BDL60 8011.7 Av. 5.59 1.37 1.18 1.111.18 BDL60 8013.4 P 0.50 0.38 0.06 0.00 0.60 BDL60 8013.4 Av. 27.18 1.131.56 1.19 1.15 BDL60 8013.6 P 0.62 0.14 0.00 0.08 BDL60 8013.6 Av. 1.181.10 1.53 1.19 BDL60 8014.5 P 0.07 0.08 BDL60 8014.5 Av. 1.38 1.17 BDL608013.6 P 0.02 BDL60 8013.6 Av. 1.12 BDL60 8014.2 P BDL60 8014.2 Av.BDL60 8014.7 P 0.07 BDL60 8014.7 Av. 1.22 BDL60 8014.8 P 0.14 0.25 0.10BDL60 8014.8 Av. 1.10 1.16 1.24 BDL65 7824.1 P 0.13 0.18 BDL65 7824.1Av. 1.44 1.22 BDL65 7825.2 P 0.00 0.05 BDL65 7825.2 Av. 1.27 1.14 BDL658473.2 P 0.01 0.01 0.00 0.00 BDL65 8473.2 Av. 1.27 1.22 1.25 1.18 BDL658474.1 P 0.01 BDL65 8474.1 Av. 1.08 BDL65 7901.5 P 0.00 0.00 0.31 0.02BDL67 7901.5 Av. 1.29 1.15 1.13 1.14 BDL67 7902.3 P 0.48 0.12 0.21 0.040.00 BDL67 7902.3 Av. 5.44 1.36 1.13 1.40 1.11 BDL67 7902.7 P 0.50 0.080.04 0.04 0.44 0.00 BDL67 7902.7 Av. 6.31 1.13 1.42 1.16 1.16 1.11 BDL677903.3 P 0.19 0.19 BDL67 7903.3 Av. 1.18 1.46 BDL67 7903.5 P 0.00 0.000.13 BDL67 7903.5 Av. 1.26 1.34 1.13 BDL68 7761.3 P 0.49 0.34 0.00 BDL687761.3 Av. 12.65 1.21 1.14 BDL68 7761.8 P 0.19 0.08 0.00 0.08 0.27 BDL687761.8 Av. 1.67 1.18 1.36 1.16 1.18 BDL68 7761.9 P 0.44 0.00 0.00 BDL687761.9 Av. 1.22 1.52 1.18 BDL68 7763.2 P 0.02 0.00 0.04 0.29 0.22 BDL687763.2 Av. 1.18 1.32 1.13 1.31 1.13 BDL68 7764.1 P 0.49 0.03 0.09 0.240.33 BDL68 7764.1 Av. 7.93 1.16 1.47 1.17 1.13 BDL78 7911.11 P 0.00 0.12BDL78 7911.11 Av. 1.20 1.14 BDL78 7911.8 P 0.07 0.05 0.08 BDL78 7911.8Av. 1.07 1.12 1.13 BDL78 7911.9 P 0.06 0.07 0.00 0.19 0.37 BDL78 7911.9Av. 1.19 1.37 1.27 1.22 1.12 BDL78 7912.6 P 0.20 0.02 0.37 BDL78 7912.6Av. 1.19 1.09 1.11 BDL78 7913.11 P 0.14 0.29 0.00 0.00 BDL78 7913.11 Av.1.13 1.11 1.19 1.15 BDL78 7913.3 P 0.03 0.04 BDL78 7913.3 Av. 1.14 1.03BDL78 7913.6 P 0.09 0.00 0.01 BDL78 7913.6 Av. 1.21 1.27 1.22 BDL787913.8 P 0.07 0.21 0.00 0.37 0.02 BDL78 7913.8 Av. 1.16 1.25 1.16 1.111.19 BDL78 7913.9 P 0.24 0.16 0.07 BDL78 7913.9 Av. 1.16 1.15 1.10 BDL827801.1 P 0.05 0.07 0.02 0.63 0.03 BDL82 7801.1 Av. 1.14 1.41 1.15 1.131.07 BDL82 7801.3 P 0.13 0.00 BDL82 7801.3 Av. 1.44 1.21 BDL82 7802.2 P0.00 0.16 BDL82 7802.2 Av. 1.17 1.12 BDL82 7802.3 P 0.32 0.13 0.00 BDL827802.3 Av. 1.14 1.30 1.16 BDL82 7803.9 P 0.54 0.28 BDL82 7803.9 Av. 1.181.35 BDL89 7812.2 P BDL89 7812.2 Av. BDL89 7812.5 P 0.51 0.18 0.11 0.180.03 BDL89 7812.5 Av. 5.84 1.18 1.26 1.12 1.04 BDL89 7814.1 P 0.58 0.000.00 BDL89 7814.1 Av. 1.16 1.17 1.04 BDL89 7814.4 P 0.46 0.05 0.36 0.21BDL89 7814.4 Av. 5.59 1.17 1.24 1.13 BDL89 7814.5 P 0.02 0.05 0.00 0.10BDL89 7814.5 Av. 1.38 1.21 1.59 1.15 Table 27.

Example 8 Evaluating Transgenic Arabidopsis Plant Growth Under AbioticStress and Nitrogen Deficiency Conditions in Tissue Culture Assay

Assay 1: plant growth under osmotic stress [poly (ethylene glycol)(PEG)] in tissue culture conditions—One of the consequences of droughtis the induction of osmotic stress in the area surrounding the roots;therefore, in many scientific studies, PEG (e.g., 1.5% PEG8000) is usedto simulate the osmotic stress conditions resembling the high osmolarityfound during drought stress.

Surface sterilized seeds were sown in basal media [50% Murashige-Skoogmedium (MS) supplemented with 0.8% plant agar as solidifying agent] inthe presence of Kanamycin (for selecting only transgenic plants). Aftersowing, plates were transferred for 2-3 days for stratification at 4° C.and then grown at 25° C. under 12-hour light 12-hour dark daily cyclesfor 7 to 10 days. At this time point, seedlings randomly chosen werecarefully transferred to plates containing 1.5% PEG: 0.5 MS media orNormal growth conditions (0.5 MS media). Each plate contained 5seedlings of the same transgenic event, and 3-4 different plates(replicates) for each event. For each polynucleotide of the invention atleast four independent transformation events were analyzed from eachconstruct. Plants expressing the polynucleotides of the invention werecompared to the average measurement of the control plants (empty vectoror GUS reporter gene under the same promoter) used in the sameexperiment.

Assay 2: plant growth at nitrogen deficiency under tissue cultureconditions—The present inventors have found the nitrogen use efficiency(NUE) assay to be relevant for the evaluation of the ABST candidategenes, since nitrogen deficiency encourages root elongation, increase ofroot coverage and allows detecting the potential of the plant togenerate a better root system under drought conditions. In addition,there are indications in the literature that biological mechanisms ofNUE and drought tolerance are linked (Wesley et al., 2002 Journal ofExperiment Botany Vol 53, No. 366, pp. 13-25).

Surface sterilized seeds were sown in basal media [50% Murashige-Skoogmedium (MS) supplemented with 0.8% plant agar as solidifying agent] inthe presence of Kanamycin (for selecting only transgenic plants). Aftersowing, plates were transferred for 2-3 days for stratification at 4° C.and then grown at 25° C. under 12-hour light 12-hour dark daily cyclesfor 7 to 10 days. At this time point, seedlings randomly chosen werecarefully transferred to plates with nitrogen-limiting conditions: 0.5MS media in which the combined nitrogen concentration (NH₄NO₃ and KNO₃)is 0.75 mM (nitrogen deficient conditions). Each plate contains 5seedlings of same event, and 3-4 different plates (replicates) for eachevent. For each polynucleotide of the invention at least fourindependent transformation events were analyzed from each construct.Plants expressing the polynucleotides of the invention were compared tothe average measurement of the control plants (empty vector or GUSreporter under the same promoter) used in the same experiment.

Digital imaging—A laboratory image acquisition system, which consists ofa digital reflex camera (Canon EOS 300D) attached with a 55 mm focallength lens (Canon EF-S series), mounted on a reproduction device(Kaiser RS), which included 4 light units (4×150 Watts light bulb) andlocated in a darkroom, was used for capturing images of plantlets sawnin agar plates.

The image capturing process was repeated every 2-5 days starting at day1 till day 10-15 (see for example the images in FIGS. 2A-B)

An image analysis system was used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.39 (Java based image processing program which was developed at theU.S. National Institutes of Health and freely available on the internetat rsbweb (dot) nih (dot) gov/). Images were captured in resolution of10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG(Joint Photographic Experts Group standard) format. Next, analyzed datawas saved to text files and processed using the JMP statistical analysissoftware (SAS institute).

Seedling analysis—Using the digital analysis seedling data wascalculated, including leaf area, root coverage and root length.

The relative growth rate for the various seedling parameters wascalculated according to the following Formulas.Relative growth rate of leaf area=(Δrosette area/Δt)*(1/rosette areat₁)  Formula VI:

Δ rosette area is the interval between the current rosette area(measured at t₂) and the rosette area measured at the previous day (Areat₁)

Δt is the time interval (t₂−t₁, in days) between the current analyzedimage day (t₂) and the previous day (t₁).

Thus, the relative growth rate of leaf area is in units of 1/day.Relative growth rate of root coverage=(Δroot coverage area/Δt)*(1/rootcoverage area t ₁)  Formula VII

Δ root coverage area is the interval between the current root coveragearea (measured at t₂) and the root coverage area measured at theprevious day (Area t₁)

Δt is the time interval (t₂−t₁, in days) between the current analyzedimage day (t₂) and the previous day (t₁).

Thus, the relative growth rate of root coverage area is in units of1/day.Relative growth rate of root length=(Δroot length/Δt)*(1/root length t₁)  Formula VIII

Δ root length is the interval between the current root length (measuredat t₂) and the root length measured at the previous day (Area t₁)

Δt is the time interval (t₂−t₁, in days) between the current analyzedimage day (t₂) and the previous day (t₁).

Thus, the relative growth rate of root length is in units of 1/day.

At the end of the experiment, plantlets were removed from the media andweighed for the determination of plant fresh weight. Plantlets were thendried for 24 hours at 60° C., and weighed again to measure plant dryweight for later statistical analysis. Growth rate was determined bycomparing the leaf area coverage, root coverage and root length, betweeneach couple of sequential photographs, and results were used to resolvethe effect of the gene introduced on plant vigor, under osmotic stress,as well as under optimal conditions. Similarly, the effect of the geneintroduced on biomass accumulation, under osmotic stress as well asunder optimal conditions, was determined by comparing the plants' freshand dry weight to that of control plants (containing an empty vector orthe GUS reporter gene under the same promoter). From every constructcreated, 3-5 independent transformation events were examined inreplicates.

Statistical analyses—To identify genes conferring significantly improvedtolerance to abiotic stresses or enlarged root architecture, the resultsobtained from the transgenic plants were compared to those obtained fromcontrol plants. To identify outperforming genes and constructs, resultsfrom the independent transformation events tested were analyzedseparately. To evaluate the effect of a gene event over a control thedata was analyzed by Student's t-test and the p value was calculated.Results were considered significant if p≦0.1. The JMP statisticssoftware package was used (Version 5.2.1, SAS Institute Inc., Cary,N.C., USA).

Experimental Results—The polynucleotide sequences of the invention wereassayed for a number of commercially desired traits. Table 28 providesthe parameters measured in a tissue culture assay (results are presentedin Tables 29 and 30). In cases where a certain event appears more thanonce, the event was tested in several independent experiments.

TABLE 28 Parameter symbol used in result Table 29 Parameter name 1 LeafArea time point 1 2 Leaf Area time point 2 3 Leaf Area time point 3 4Roots Length time point 1 5 Roots Length time point 2 6 Roots Lengthtime point 3 7 Roots Coverage time point 1 8 Roots Coverage time point 29 Roots Coverage time point 3 10 RGR of Leaf Area time point 2 11 RGR ofLeaf Area time point 3 12 RGR of Roots Coverage time point 2 13 RGR ofRoots Coverage time point 3 14 RGR of Roots Length time point 2 15 RGRof Roots Length time point 3 16 Fresh Weight 17 Dry Weight Table 28.

TABLE 29 p.n. SEQ ID Gene NO: Ev. Par. 1 2 3 4 5 6 7 8 9 BDL100 6577872.2 P BDL100 657 7872.2 Av BDL100 657 7872.3 P BDL100 657 7872.3 AvBDL100 657 7873.2 P BDL100 657 7873.2 Av BDL100 657 7873.4 P 0.28 0.320.13 0.33 0.21 0.36 0.47 BDL100 657 7873.4 Av 1.16 1.18 1.39 1.17 1.411.19 1.12 BDL51 694 8021.1 P BDL51 694 8021.1 Av BDL51 694 8022.4 PBDL51 694 8022.4 Av BDL51 694 8022.5 P BDL51 694 8022.5 Av BDL51 6948024.4 P BDL51 694 8024.4 Av BDL51 694 8024.7 P BDL51 694 8024.7 AvBDL82 704 7801.3 P 0.23 BDL82 704 7801.3 Av 1.18 BDL82 704 7802.2 PBDL82 704 7802.2 Av BDL82 704 7802.3 P BDL82 704 7802.3 Av BDL82 7047803.8 P BDL82 704 7803.8 Av BDL82 704 7803.9 P BDL82 704 7803.9 AvBDL89 705 7812.2 P BDL89 705 7812.2 Av BDL89 705 7812.5 P BDL89 7057812.5 Av BDL89 705 7814.1 P BDL89 705 7814.1 Av BDL89 705 7814.4 PBDL89 705 7814.4 Av BDL89 705 7814.5 P BDL89 705 7814.5 Av BDL95_Short706 7841.2 P BDL95_Short 706 7841.2 Av BDL95_Short 706 7842.12 PBDL95_Short 706 7842.12 Av BDL95_Short 706 7842.2 P BDL95_Short 7067842.2 Av BDL95_Short 706 7842.8 P BDL95_Short 706 7842.8 Av BDL95_Short706 7843.4 P BDL95_Short 706 7843.4 Av BDL108 659 8122.1 P BDL108 6598122.1 Av BDL108 659 8122.2 P BDL108 659 8122.2 Av BDL108 659 8123.6 PBDL108 659 8123.6 Av BDL108 659 8123.5 P BDL108 659 8123.5 Av BDL59 6987792.1 P BDL59 698 7792.1 Av BDL59 698 7792.2 P BDL59 698 7792.2 AvBDL59 698 7792.3 P BDL59 698 7792.3 Av BDL59 698 7793.3 P BDL59 6987793.3 Av BDL59 698 7794.1 P BDL59 698 7794.1 Av BDL68 702 7761.3 PBDL68 702 7761.3 Av BDL68 702 7761.5 P BDL68 702 7761.5 Av BDL68 7027761.9 P BDL68 702 7761.9 Av BDL68 702 7764.1 P BDL68 702 7764.1 Av p.n.SEQ ID Gene NO: Ev. Par. 10 11 12 13 14 15 16 17 BDL100 657 7872.2 P0.29 0.10 0.53 0.08 0.54 0.03 BDL100 657 7872.2 Av 1.55 1.21 1.66 1.951.49 1.63 BDL100 657 7872.3 P 0.60 0.08 0.12 0.18 BDL100 657 7872.3 Av1.10 1.13 1.71 1.51 BDL100 657 7873.2 P 0.41 0.12 0.01 0.00 BDL100 6577873.2 Av 1.23 1.12 1.61 1.50 BDL100 657 7873.4 P 0.01 0.45 0.48 BDL100657 7873.4 Av 1.28 1.22 1.19 BDL51 694 8021.1 P 0.13 0.02 0.08 BDL51 6948021.1 Av 1.23 2.20 1.47 BDL51 694 8022.4 P 0.00 0.09 0.05 BDL51 6948022.4 Av 1.32 1.97 1.49 BDL51 694 8022.5 P 0.08 0.04 BDL51 694 8022.5Av 1.14 1.49 BDL51 694 8024.4 P 0.36 0.00 0.16 BDL51 694 8024.4 Av 1.412.12 1.55 BDL51 694 8024.7 P 0.34 0.23 BDL51 694 8024.7 Av 1.29 1.28BDL82 704 7801.3 P 0.00 0.04 0.00 BDL82 704 7801.3 Av 1.51 2.15 1.67BDL82 704 7802.2 P 0.01 0.00 0.01 BDL82 704 7802.2 Av 1.24 2.69 1.98BDL82 704 7802.3 P 0.02 0.11 0.03 BDL82 704 7802.3 Av 1.24 1.57 1.42BDL82 704 7803.8 P 0.15 0.16 0.12 0.04 0.02 BDL82 704 7803.8 Av 1.292.22 1.63 1.41 1.33 BDL82 704 7803.9 P BDL82 704 7803.9 Av BDL89 7057812.2 P 0.30 0.05 0.58 0.05 0.38 0.01 BDL89 705 7812.2 Av 1.19 1.501.52 2.31 1.41 1.65 BDL89 705 7812.5 P 0.05 0.01 0.00 BDL89 705 7812.5Av 1.32 3.25 2.10 BDL89 705 7814.1 P 0.60 0.00 0.24 0.01 BDL89 7057814.1 Av 1.13 1.40 2.18 1.70 BDL89 705 7814.4 P 0.07 0.33 0.07 0.07BDL89 705 7814.4 Av 1.35 1.19 1.74 1.52 BDL89 705 7814.5 P 0.08 0.190.33 0.30 0.20 0.36 BDL89 705 7814.5 Av 1.29 1.26 1.79 1.56 1.56 1.30BDL95_Short 706 7841.2 P 0.14 0.66 0.14 0.65 0.04 BDL95_Short 706 7841.2Av 1.22 1.29 1.42 1.17 1.27 BDL95_Short 706 7842.12 P 0.30 0.05 0.040.07 0.20 BDL95_Short 706 7842.12 Av 1.14 1.17 1.36 1.19 1.19BDL95_Short 706 7842.2 P 0.15 0.29 0.08 BDL95_Short 706 7842.2 Av 1.451.27 1.28 BDL95_Short 706 7842.8 P 0.35 0.00 0.07 0.07 BDL95_Short 7067842.8 Av 1.10 1.37 2.82 2.07 BDL95_Short 706 7843.4 P 0.43 0.00 0.010.01 0.41 BDL95_Short 706 7843.4 Av 1.14 1.42 3.00 2.12 1.26 BDL108 6598122.1 P 0.11 0.18 0.45 0.61 0.73 0.51 BDL108 659 8122.1 Av 1.28 1.241.44 1.13 1.16 1.20 BDL108 659 8122.2 P 0.09 0.02 0.00 0.00 0.12 0.010.77 0.38 BDL108 659 8122.2 Av 1.59 1.37 1.82 2.09 1.29 1.71 1.11 1.17BDL108 659 8123.6 P 0.59 0.57 0.40 0.08 BDL108 659 8123.6 Av 1.10 1.331.27 1.53 BDL108 659 8123.5 P 0.04 0.29 0.25 BDL108 659 8123.5 Av 1.141.75 1.36 BDL59 698 7792.1 P 0.26 0.70 BDL59 698 7792.1 Av 1.38 1.11BDL59 698 7792.2 P 0.33 0.31 0.02 0.25 0.14 0.01 BDL59 698 7792.2 Av1.33 1.23 1.63 1.26 1.28 1.40 BDL59 698 7792.3 P 0.02 0.11 0.29 BDL59698 7792.3 Av 1.36 1.43 1.28 BDL59 698 7793.3 P 0.06 0.32 0.30 0.16 0.110.05 BDL59 698 7793.3 Av 1.76 1.19 1.99 1.56 1.65 1.27 BDL59 698 7794.1P 0.18 0.11 0.13 BDL59 698 7794.1 Av 1.15 1.83 1.35 BDL68 702 7761.3 P0.32 0.46 BDL68 702 7761.3 Av 1.15 1.17 BDL68 702 7761.5 P 0.40 0.150.21 0.14 0.20 BDL68 702 7761.5 Av 2.43 1.86 1.58 1.60 1.42 BDL68 7027761.9 P BDL68 702 7761.9 Av BDL68 702 7764.1 P 0.00 0.53 0.22 BDL68 7027764.1 Av 1.44 1.45 1.61 Table 29. Provided are the growth and biomassparameters of transgenic vs. control plants as measured in Tssue Cultureassay under 1.5% PEG. “P” = P-value; “Av” = ratio between the averagesof event and control. Note that when the average ratio is higher than“1” the effect of exogenous expression of the gene is an increase of thedesired trait; “Par” = Parameter according to the parameters listed inTable 28 above; “Ev” = event. “p.n.” = polynucleotide.

TABLE 30 p.n. SEQ ID Gene NO: Ev. Par. 1 2 3 4 5 6 7 8 9 BDL100 6577872.2 P BDL100 657 7872.2 Av BDL100 657 7872.3 P 0.40 BDL100 657 7872.3Av 1.13 BDL100 657 7873.2 P BDL100 657 7873.2 Av BDL100 657 7873.3 P0.60 0.36 0.56 BDL100 657 7873.3 Av 1.15 1.12 1.27 BDL100 657 7873.4 P0.24 0.03 0.03 0.00 0.01 0.33 BDL100 657 7873.4 Av 1.25 1.36 1.39 1.591.96 1.12 BDL51 694 8021.1 P BDL51 694 8021.1 Av BDL51 694 8022.4 P 0.51BDL51 694 8022.4 Av 1.31 BDL51 694 8022.5 P 0.02 0.09 BDL51 694 8022.5Av 1.24 1.30 BDL51 694 8024.4 P BDL51 694 8024.4 Av BDL51 694 8024.7 P0.55 0.04 0.09 0.05 0.24 BDL51 694 8024.7 Av 1.11 1.48 1.09 2.30 1.12BDL82 704 7801.1 P BDL82 704 7801.1 Av BDL82 704 7801.3 P BDL82 7047801.3 Av BDL82 704 7802.2 P BDL82 704 7802.2 Av BDL82 704 7802.3 P 0.52BDL82 704 7802.3 Av 1.14 BDL82 704 7803.8 P BDL82 704 7803.8 Av BDL82704 7803.9 P BDL82 704 7803.9 Av BDL89 705 7812.2 P BDL89 705 7812.2 AvBDL89 705 7812.5 P BDL89 705 7812.5 Av BDL89 705 7814.1 P BDL89 7057814.1 Av BDL89 705 7814.4 P 0.19 0.30 BDL89 705 7814.4 Av 1.19 1.21BDL89 705 7814.5 P 0.42 BDL89 705 7814.5 Av 1.11 BDL95_Short 706 7841.2P BDL95_Short 706 7841.2 Av BDL95_Short 706 7842.12 P 0.28 0.17 0.190.22 BDL95_Short 706 7842.12 Av 1.15 1.13 1.17 1.26 BDL95_Short 7067842.2 P 0.17 BDL95_Short 706 7842.2 Av 1.32 BDL95_Short 706 7842.8 PBDL95_Short 706 7842.8 Av BDL95_Short 706 7843.4 P BDL95_Short 7067843.4 Av BDL108 659 8122.1 P BDL108 659 8122.1 Av BDL108 659 8122.2 P0.08 0.00 0.08 BDL108 659 8122.2 Av 1.10 1.22 1.24 BDL108 659 8123.6 PBDL108 659 8123.6 Av BDL108 659 8123.5 P BDL108 659 8123.5 Av BDL59 6987792.1 P BDL59 698 7792.1 Av BDL59 698 7792.2 P BDL59 698 7792.2 AvBDL59 698 7792.3 P BDL59 698 7792.3 Av BDL59 698 7793.3 P 0.33 BDL59 6987793.3 Av 1.12 BDL59 698 7794.1 P BDL59 698 7794.1 Av BDL68 702 7761.3 P0.22 0.26 BDL68 702 7761.3 Av 1.20 1.15 BDL68 702 7761.5 P BDL68 7027761.5 Av BDL68 702 7761.9 P BDL68 702 7761.9 Av BDL68 702 7764.1 PBDL68 702 7764.1 Av p.n. SEQ ID Gene NO: Ev. Par. 10 11 12 13 14 15 1617 BDL100 657 7872.2 P 0.16 0.11 0.08 0.01 BDL100 657 7872.2 Av 1.361.58 2.18 1.65 BDL100 657 7872.3 P 0.19 0.02 0.03 BDL100 657 7872.3 Av1.13 1.96 1.60 BDL100 657 7873.2 P 0.03 0.00 0.00 BDL100 657 7873.2 Av1.52 2.08 1.72 BDL100 657 7873.3 P 0.52 0.15 0.02 0.01 BDL100 657 7873.3Av 1.12 1.23 1.32 1.23 BDL100 657 7873.4 P 0.21 0.23 0.16 0.02 BDL100657 7873.4 Av 1.18 1.22 1.13 1.42 BDL51 694 8021.1 P 0.06 0.00 0.00BDL51 694 8021.1 Av 1.33 2.61 1.60 BDL51 694 8022.4 P 0.03 0.02 0.14BDL51 694 8022.4 Av 1.17 2.13 1.46 BDL51 694 8022.5 P 0.08 0.00 0.020.06 BDL51 694 8022.5 Av 1.22 1.36 2.42 1.75 BDL51 694 8024.4 P 0.000.35 0.07 BDL51 694 8024.4 Av 1.32 3.10 1.86 BDL51 694 8024.7 P 0.180.38 0.23 BDL51 694 8024.7 Av 1.24 1.19 1.15 BDL82 704 7801.1 P 0.110.02 0.14 BDL82 704 7801.1 Av 1.26 3.23 1.76 BDL82 704 7801.3 P 0.130.00 0.00 BDL82 704 7801.3 Av 1.27 3.07 1.76 BDL82 704 7802.2 P 0.140.01 0.00 BDL82 704 7802.2 Av 1.37 5.55 2.69 BDL82 704 7802.3 P 0.430.41 0.50 BDL82 704 7802.3 Av 1.11 1.17 1.17 BDL82 704 7803.8 P 0.010.00 BDL82 704 7803.8 Av 2.16 1.77 BDL82 704 7803.9 P 0.61 0.12 0.010.53 BDL82 704 7803.9 Av 1.11 1.81 1.49 1.14 BDL89 705 7812.2 P 0.290.00 0.00 0.30 0.18 BDL89 705 7812.2 Av 1.13 1.30 1.38 1.11 1.16 BDL89705 7812.5 P 0.03 0.02 0.01 BDL89 705 7812.5 Av 1.39 5.28 2.83 BDL89 7057814.1 P 0.00 0.01 0.00 BDL89 705 7814.1 Av 1.45 1.84 1.38 BDL89 7057814.4 P 0.03 0.39 0.01 0.17 BDL89 705 7814.4 Av 1.61 1.16 1.49 1.14BDL89 705 7814.5 P 0.29 0.04 0.00 0.03 BDL89 705 7814.5 Av 1.20 1.361.74 1.59 BDL95_Short 706 7841.2 P 0.07 0.00 0.00 BDL95_Short 706 7841.2Av 1.31 2.75 1.81 BDL95_Short 706 7842.12 P 0.44 0.02 0.11 0.43 0.64BDL95_Short 706 7842.12 Av 1.14 1.24 1.16 1.25 1.11 BDL95_Short 7067842.2 P 0.19 0.02 0.00 BDL95_Short 706 7842.2 Av 1.17 2.06 1.61BDL95_Short 706 7842.8 P 0.17 0.03 0.00 0.00 BDL95_Short 706 7842.8 Av1.14 1.18 2.17 1.44 BDL95_Short 706 7843.4 P 0.01 0.01 0.00 BDL95_Short706 7843.4 Av 1.67 4.58 2.59 BDL108 659 8122.1 P 0.32 0.31 BDL108 6598122.1 Av 1.25 1.91 BDL108 659 8122.2 P 0.01 0.27 0.00 0.05 0.00 0.02BDL108 659 8122.2 Av 2.01 1.16 1.81 1.50 1.55 1.39 BDL108 659 8123.6 P0.15 0.02 0.09 0.07 0.07 BDL108 659 8123.6 Av 1.25 1.56 1.57 1.21 1.36BDL108 659 8123.5 P 0.18 0.19 BDL108 659 8123.5 Av 1.62 1.40 BDL59 6987792.1 P 0.11 BDL59 698 7792.1 Av 1.50 BDL59 698 7792.2 P 0.17 0.21 0.160.33 0.50 BDL59 698 7792.2 Av 1.31 1.31 1.62 1.11 1.17 BDL59 698 7792.3P 0.01 0.27 BDL59 698 7792.3 Av 1.73 1.18 BDL59 698 7793.3 P 0.03 0.010.03 0.02 0.04 BDL59 698 7793.3 Av 1.89 1.75 1.87 1.39 1.40 BDL59 6987794.1 P 0.14 0.08 0.29 BDL59 698 7794.1 Av 1.22 1.59 1.18 BDL68 7027761.3 P 0.14 0.17 0.34 BDL68 702 7761.3 Av 1.32 1.38 1.13 BDL68 7027761.5 P 0.30 0.02 0.46 0.02 BDL68 702 7761.5 Av 1.56 1.71 1.22 1.59BDL68 702 7761.9 P 0.33 BDL68 702 7761.9 Av 1.30 BDL68 702 7764.1 P 0.080.15 0.38 BDL68 702 7764.1 Av 1.27 1.77 1.21 Table 30. Provided are thegrowth and biomass parameters of transgenic vs. control plants asmeasured in Tissue Calture assay under 0.75 mM Nitrogen concentration.“P” = P-value; “Av” = ratio between the averages of event and control.Note that when the average ratio is higher than “1” the effect ofexogenous expression of the gene is an increase of the desired trait;“Par” = Parameter according to the parameters listed in Table 28 above;“Ev” = event.

Example 9 Improving Desired Traits of Interest in Transgenic PlantsGrown Under Normal Conditions by Reducing Gene Expression

Transgenic plants exogenously expressing the BDL127 gene (SEQ ID NO:673)were assayed for a number of commercially desired traits under normalconditions.

To analyze the effect of expression of the BDL127 exogenouspolynucleotide in transgenic plants, plants were grown in pots with anadequate amount of nutrients and water. The plants were evaluated usingvarious parameters for their overall size (biomass), structure (plantarchitecture), relative growth rate, time to inflorescence emergence(bolting) and flowering, seed yield, weight of 1,000 seeds, dry matter,oil content and harvest index [(HI) seed yield/dry matter]. Transgenicplants performance was compared to control plants grown in parallelunder the same conditions. Mock-transgenic plants with an empty vectoror expressing the uidA reporter gene (GUS-Intron) under the samepromoter were used as control.

Parameters were measured as described in Example 3 above.

Statistical analyses—All parameters including plant growth rate, plantarea, biomass, plant architecture, time to bolting, time to flowering,weight of 1,000 seeds, seed yield, oil yield, dry matter, and harvestindex area data were analyzed using t-test. To identify outperforminggenes and constructs, results from mix of transformation events orindependent events were analyzed. For gene versus control analysist-test was applied, using significance threshold of p<0.1.

Experimental Results

The polynucleotide sequences of the invention were assayed for a numberof commercially desired traits. Table 22 provides the parametersmeasured in a tissue culture and green house assays (results arepresented in Tables 31 and 32). In cases where a certain event appearsmore than once, the event was tested in several independent experiments.

Analysis of plants in tissue culture assay—Table 31, hereinbelow,depicts analyses of transgenic plants overexpressing the BDL127polynucleotide of the invention under the regulation of the constitutive35S (SEQ ID NO:777) promoter.

TABLE 31 Gene Ev. Par. 1 2 3 4 5 6 7 8 9 BDL127 8172.1 P 8.5E−03 1.5E−032.0E−03 2.4E−04 1.4E−03 1.6E−01 1.3E−03 4.7E−03 1.2E−01 BDL127 8172.1 Av7.4E−01 6.1E−01 6.3E−01 2.8E−01 5.1E−01 6.5E−01 1.7E−01 3.6E−01 5.3E−01BDL127 8172.4 P 4.5E−03 1.9E−05 3.3E−03 7.4E−04 2.2E−05 2.3E−04 2.4E−039.2E−04 4.1E−03 BDL127 8172.4 Av 7.7E−01 6.2E−01 7.0E−01 4.1E−01 3.9E−015.2E−01 2.7E−01 2.1E−01 3.1E−01 BDL127 8172.7 P 1.8E−01 3.8E−04 2.0E−033.4E−02 1.8E−03 1.7E−02 7.9E−02 BDL127 8172.7 Av 8.4E−01 3.5E−01 6.0E−016.7E−01 2.3E−01 5.3E−01 6.0E−01 BDL127 8172.8 P 4.8E−01 3.5E−01 3.1E−032.7E−01 2.2E−01 BDL127 8172.8 Av 8.9E−01 8.5E−01 5.4E−01 8.0E−01 6.0E−01BDL127 8172.9 P 3.3E−03 1.8E−05 3.0E−03 3.1E−04 2.3E−05 4.5E−05 1.5E−036.5E−04 1.2E−03 BDL127 8172.9 Av 7.9E−01 5.2E−01 5.4E−01 3.4E−01 3.4E−013.7E−01 2.1E−01 1.6E−01 1.4E−01 Gene Ev. Par. 10 11 12 13 14 15 16 17BDL127 8172.1 P 2.6E−02 3.8E−01 2.3E−02 BDL127 8172.1 Av 7.5E−01 8.7E−017.6E−01 BDL127 8172.4 P 1.6E−02 4.9E−01 2.0E−02 BDL127 8172.4 Av 7.3E−018.1E−01 7.6E−01 BDL127 8172.7 P 2.9E−01 2.8E−01 BDL127 8172.7 Av 8.9E−017.5E−01 BDL127 8172.8 P 5.4E−01 3.8E−01 3.1E−01 BDL127 8172.8 Av 9.0E−018.3E−01 8.7E−01 BDL127 8172.9 P 1.3E−03 2.0E−01 7.4E−01 6.1E−02 4.9E−02BDL127 8172.9 Av 5.1E−01 6.9E−01 9.0E−01 7.3E−01 6.4E−01 Table 31. “P” =P-value; “Av” = ratio between the averages of event and control. Notethat when the average ratio is less than “1” the effect of exogenousexpression of the gene is a decrease of the desired trait; “Par” =Parameter according to the parameters listed in Table 22 above; “Ev” =event.

Greenhouse assays—Tables 33-36 represent experiments that were doneusing greenhouse assays. Table 32 specifies the parameters that weremeasured in the green house assays and which are presented in Tables33-36. In cases where a certain event appears more than once, the eventwas tested in several independent experiments.

TABLE 32 Parameter symbol used in result Tables 33-36 Parameter name 1Rosette Diameter time point 1 2 Rosette Diameter time point 2 3 RosetteDiameter time point 3 4 Rosette Diameter time point 4 5 Rosette Areatime point 1 6 Rosette Area time point 2 7 Rosette Area time point 3 8Rosette Area time point 4 9 Plot Coverage time point 1 10 Plot Coveragetime point 2 11 Plot Coverage time point 3 12 Plot Coverage time point 413 Leaf Number time point 1 14 Leaf Number time point 2 15 Leaf Numbertime point 3 16 Leaf Number time point 4 17 Leaf Blade Area time point 118 Leaf Blade Area time point 2 19 Leaf Blade Area time point 3 20 LeafBlade Area time point 4 21 Leaf Petiole Area time point 1 22 LeafPetiole Area time point 2 23 Leaf Petiole Area time point 3 24 LeafPetiole Area time point 4 25 Blade Relative Area time point 1 26 BladeRelative Area time point 2 27 Blade Relative Area time point 3 28 BladeRelative Area time point 4 29 Petiole Relative Area time point 1 30Petiole Relative Area time point 2 31 Petiole Relative Area time point 332 Petiole Relative Area time point 4 33 RGR of Leaf Blade Area timepoint 2 34 RGR of Leaf Blade Area time point 3 35 RGR of Leaf Blade Areatime point 4 36 RGR of Leaf Number time point 2 37 RGR of Leaf Numbertime point 3 38 RGR of Leaf Number time point 4 39 RGR of Rosette Areatime point 2 40 RGR of Rosette Area time point 3 41 RGR of Rosette Areatime point 4 42 RGR of Rosette Diameter time point 2 43 RGR of RosetteDiameter time point 3 44 RGR of Rosette Diameter time point 4 45 RGR ofPlot Coverage time point 2 46 RGR of Plot Coverage time point 3 47 RGRof Plot Coverage time point 4 48 Bolting 49 Flowering 50 Dry Weight 51Seed Yield 52 Harvest Index 53 1000 Seeds Weight 54 oil content 55 FreshWeight Table 32.

TABLE 33 Gene Ev Par 1 2 3 4 5 6 7 8 BDL127 8172.10 P BDL127 8172.10 AvBDL127 8172.4 P 1.2E−03 1.7E−03 7.1E−03 1.7E−03 5.9E−03 1.6E−03 BDL1278172.4 Av 7.6E−01 7.7E−01 7.1E−01 2.0E−01 6.7E−01 6.5E−01 BDL127 8172.7P BDL127 8172.7 Av BDL127 8172.8 P 8.0E−04 2.2E−04 1.8E−02 1.2E−021.7E−02 3.3E−02 BDL127 8172.8 Av 7.4E−01 7.0E−01 6.2E−01 5.7E−01 5.2E−015.2E−01 BDL127 8172.9 P 2.1E−02 6.4E−03 8.8E−04 7.1E−04 1.1E−02 4.4E−04BDL127 8172.9 Av 8.0E−01 7.1E−01 7.3E−01 7.3E−01 6.1E−01 5.6E−01 Gene EvPar 9 10 11 12 13 14 15 16 BDL127 8172.10 P BDL127 8172.10 Av BDL1278172.4 P 7.1E−03 1.7E−03 5.9E−03 1.6E−03 BDL127 8172.4 Av 7.1E−012.0E−01 6.7E−01 6.5E−01 BDL127 8172.7 P BDL127 8172.7 Av BDL127 8172.8 P1.8E−02 1.2E−02 1.7E−02 3.3E−02 3.6E−04 BDL127 8172.8 Av 6.2E−01 5.7E−015.2E−01 5.2E−01 7.8E−01 BDL127 8172.9 P 1.1E−02 4.4E−04 4.5E−02 BDL1278172.9 Av 6.1E−01 5.6E−01 8.5E−01 Table 33. “P” = P-value; “Av” = ratiobetween the averages of event and control. Note that when the averageratio is less than “1” the effect of exogenous expression of the gene isa decrease of the desired trait; “Par” = Parameter according to theparameters listed in Table 22 above; “Ev” = event.

TABLE 34 Gene Ev Par 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32BDL127 8172.10 P BDL127 8172.10 Av BDL127 8172.4 P 5.4E−04 2.0E−037.7E−03 BDL127 8172.4 Av 3.3E−01 6.9E−01 6.3E−01 BDL127 8172.7 P 1.8E−023.1E−02 BDL127 8172.7 Av 6.8E−01 8.0E−01 BDL127 8172.8 P 3.3E−03 1.4E−032.1E−02 BDL127 8172.8 Av 6.9E−01 6.2E−01 5.6E−01 BDL127 8172.9 P 3.8E−04BDL127 8172.9 Av 5.7E−01 Table 34. “P” = P-value; “Av” = ratio betweenthe averages of event and control. Note that when the average ratio isless than “1” the effect of exogenous expression of the gene is adecrease of the desired trait; “Par” = Parameter according to theparameters listed in Table 22 above; “Ev” = event.

TABLE 35 Gene Ev Par 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48BDL127 8172.10 P BDL127 8172.10 Av BDL127 8172.4 P 3.0E−07 4.1E−024.1E−02 BDL127 8172.4 Av −1.1E−02 −5.2E−02 −5.2E−02 BDL127 8172.7 PBDL127 8172.7 Av BDL127 8172.8 P BDL127 8172.8 Av BDL127 8172.9 P3.4E−02 2.1E−02 3.4E−02 BDL127 8172.9 Av 8.0E−01 7.4E−01 8.0E−01 Table35. “P” = P-value; “Av” = ratio between the averages of event andcontrol. Note that when the average rato is less than “1” the effect ofexogenous expression of the gene is a decrease of the desired trait;“Par” = Parameter according to the parameters listed in Table 22 above;“Ev” = event.

TABLE 36 Gene Ev Par 49 50 51 52 53 54 55 BDL127 8172.10 P BDL1278172.10 Av BDL127 8172.4 P 2.0E−02 BDL127 8172.4 Av 6.9E−01 BDL1278172.7 P 2.0E−02 BDL127 8172.7 Av 7.3E−01 BDL127 8172.8 P BDL127 8172.8Av BDL127 8172.9 P 3.1E−02 BDL127 8172.9 Av 8.1E−01 Table 36. “P” =P-value; “Av” = ratio between the averages of event and control. Notethat when the average ratio is less than “1” the effect of exogenousexpression of the gene is a decrease of the desired trait; “Par” =Parameter according to the parameters listed in Table 22 above; “Ev” =event.

These results demonstrate that transformation of plants with the BDL127gene results in decreased yield, seed yield, biomass and growth rate andthus agents which downregulate the expression level of the BDL127 genein plants such as co-suppression agents, antisense suppression, RNAinterference, remodeling of the promoter structure and/or Ribozyme canincrease yield, seed yield, biomass and growth rate of a plant.

The genes identified herein improve plant yield in general, and morespecifically oil yield, seed yield, oil content, plant growth rate,plant biomass, root measurements, ABST, NUE and plant vigor. The outputof the bioinformatics method described herein is a set of genes highlypredicted to improve yield (seed yield, oil yield and content, biomass)and/or other agronomic important yields by modifying their expression.Although each gene is predicted to have its own impact, modifying themode of expression of more than one gene is expected to provide anadditive or synergistic effect on the plant yield, plant growth rate,root measurements, ABST, NUE, plant vigor and/or other agronomicimportant yields performance. Altering the expression of each genedescribed here alone or set of genes together increases the overallyield plant growth rate, root measurements, ABST, NUE, plant vigorand/or other agronomic important yields performance.

Although the invention has been described in conjunction with specificembodiments thereof, it is evident that many alternatives, modificationsand variations will be apparent to those skilled in the art.Accordingly, it is intended to embrace all such alternatives,modifications and variations that fall within the spirit and broad scopeof the appended claims.

All publications, patents and patent applications mentioned in thisspecification are herein incorporated in their entirety by referenceinto the specification, to the same extent as if each individualpublication, patent or patent application was specifically andindividually indicated to be incorporated herein by reference. Inaddition, citation or identification of any reference in thisapplication shall not be construed as an admission that such referenceis available as prior art to the present invention. To the extent thatsection headings are used, they should not be construed as necessarilylimiting.

What is claimed is:
 1. A method of increasing oil in a plant,comprising: (a) providing a plant transformed with a nucleic acidconstruct which comprises the nucleic acid sequence set forth in SEQ IDNOs:18 or 674, wherein said nucleic acid sequence encodes thepolypeptide set forth in SEQ ID NO:68; (b) expressing the polypeptide ofSEQ ID NO: 68 in said transformed plant, and (c) extracting the oil fromthe transformed plant, and wherein said transformed plant has increasedoil as compared to a wild-type plant of the same species.
 2. The methodof claim 1, wherein said nucleic acid sequence is operably linked to aheterologous promoter for directing transcription of said nucleic acidsequence in a plant cell of said transformed plant.
 3. The method ofclaim 2, wherein said promoter comprises a nucleic acid sequenceselected from the group consisting of SEQ ID NOs:779, 780, 781, 782,783, 784, 785, 786, 787, 788, 789, 790, 791 and
 792. 4. A method ofincreasing oil content, yield, growth rate, biomass, or tolerancesalinity stress of a plant as compared to a wild type plant of the samespecies which is grown under the same growth conditions, comprising: (a)transforming plants with an exogenous polynucleotide comprising anucleic acid sequence encoding the polypeptide set forth in SEQ ID NO:68; and (b) selecting a transformed plant from step (a) that expressesthe tide of SEQ ID NO: 68 and exhibits increased oil content, increasedyield, increased growth rate, increased biomass, or increased toleranceto salinity stress as compared to a wild type plant of the same specieswhich is grown under the same growth conditions.
 5. The method of claim4, wherein said nucleic acid sequence encoding the polypeptide of SEQ IDNO: 68 set forth in SEQ ID NO: 18 or SEQ ID NO:
 674. 6. The method ofclaim 1, further comprising growing the transformed plant expressingsaid exogenous polynucleotide encoding said polypeptide of SEQ ID NO: 68under the salinity stress condition.
 7. A method of producing a cropplant comprising: (a) transforming parent plants with an exogenouspolynucleotide encoding the polypeptide set forth in SEQ ID NO:68; (b)obtaining a population of transgenic crop plants from step (a); and (c)selecting a transformed crop plant from step (b) which expresses thepolypeptide of SEQ ID NO:68 encoded by said exogenous polynucleotide,and exhibits increased oil content, increased yield, increased growthrate, increased biomass, or increased tolerance to salinity stress ascompared to a wild type plant of the same species which is grown underthe same growth conditions.
 8. The method of claim 7, wherein thetransformed crop plant of step (c) is selected for increased toleranceto salinity stress as compared to a wild type plant of the same specieswhich is grown under the same growth conditions.
 9. The method of claim7, wherein the transformed crop plant of step (c) is selected forincreased oil content as compared to a wild type plant of the samespecies which is grown under the same growth conditions.
 10. The methodof claim 7, wherein the transformed crop plant of step (c) is selectedfor increased biomass as compared to a wild type plant of the samespecies which is grown under the same growth conditions.